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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
TRA-1-81 (Podocalyxin) Monoclonal Antibody (TRA-1-81), eBioscience™
catalog :
14-8883-82
quantity :
100 µg
price :
US 306
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
TRA-1-81
reactivity :
human, rhesus macaque
application :
western blot, immunocytochemistry, immunoprecipitation, flow cytometry
more info or order :
citations: 39
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:100; loading ...; fig s2c
Han Y, Tan L, Zhou T, Yang L, Carrau L, Lacko L, et al. A human iPSC-array-based GWAS identifies a virus susceptibility locus in the NDUFA4 gene and functional variants. Cell Stem Cell. 2022;29:1475-1490.e6 pubmed publisher
  • immunocytochemistry; rhesus macaque; 1:100; fig 2a
Rodriguez Polo I, Mißbach S, Petkov S, Mattern F, Maierhofer A, Grządzielewska I, et al. A piggyBac-based platform for genome editing and clonal rhesus macaque iPSC line derivation. Sci Rep. 2021;11:15439 pubmed publisher
  • flow cytometry; human; 1:100
Gkountela S, Li Z, Chin C, Lee S, Clark A. PRMT5 is required for human embryonic stem cell proliferation but not pluripotency. Stem Cell Rev. 2014;10:230-9 pubmed publisher
Hathy E, Szabó E, Vincze K, Haltrich I, Kiss E, Varga N, et al. Generation of multiple iPSC clones from a male schizophrenia patient carrying de novo mutations in genes KHSRP, LRRC7, and KIR2DL1, and his parents. Stem Cell Res. 2021;51:102140 pubmed publisher
Petkov S, Dressel R, Rodriguez Polo I, Behr R. Controlling the Switch from Neurogenesis to Pluripotency during Marmoset Monkey Somatic Cell Reprogramming with Self-Replicating mRNAs and Small Molecules. Cells. 2020;9: pubmed publisher
Matsumura K, Seiriki K, Okada S, Nagase M, Ayabe S, Yamada I, et al. Pathogenic POGZ mutation causes impaired cortical development and reversible autism-like phenotypes. Nat Commun. 2020;11:859 pubmed publisher
Tang C, Zhou D, Tan R, Zhong X, Xiao X, Qin D, et al. Auxiliary genetic analysis in a Chinese adolescent NPH family by single nucleotide polymorphism screening. Mol Med Rep. 2020;21:1115-1124 pubmed publisher
Chumarina M, Russ K, Azevedo C, Heuer A, Pihl M, Collin A, et al. Cellular alterations identified in pluripotent stem cell-derived midbrain spheroids generated from a female patient with progressive external ophthalmoplegia and parkinsonism who carries a novel variation (p.Q811R) in the POLG1 gene. Acta Neuropathol Commun. 2019;7:208 pubmed publisher
Giesselmann P, Brändl B, Raimondeau E, Bowen R, Rohrandt C, Tandon R, et al. Analysis of short tandem repeat expansions and their methylation state with nanopore sequencing. Nat Biotechnol. 2019;37:1478-1481 pubmed publisher
Wilkinson A, Ryan D, Kucinski I, Wang W, Yang J, Nestorowa S, et al. Expanded potential stem cell media as a tool to study human developmental hematopoiesis in vitro. Exp Hematol. 2019;76:1-12.e5 pubmed publisher
De Los Angeles A, Sakurai M, Wu J. Embryonic Chimeras with Human Pluripotent Stem Cells. Methods Mol Biol. 2019;2005:125-151 pubmed publisher
Wang S, Liu Z, Ye Y, Li B, Liu T, Zhang W, et al. Ectopic hTERT expression facilitates reprograming of fibroblasts derived from patients with Werner syndrome as a WS cellular model. Cell Death Dis. 2018;9:923 pubmed publisher
Weltner J, Balboa D, Katayama S, Bespalov M, Krjutskov K, Jouhilahti E, et al. Human pluripotent reprogramming with CRISPR activators. Nat Commun. 2018;9:2643 pubmed publisher
Thekkeparambil Chandrabose S, Sriram S, Subramanian S, Cheng S, Ong W, Rozen S, et al. Amenable epigenetic traits of dental pulp stem cells underlie high capability of xeno-free episomal reprogramming. Stem Cell Res Ther. 2018;9:68 pubmed publisher
Kondrashov A, Duc Hoang M, Smith J, Bhagwan J, Duncan G, Mosqueira D, et al. Simplified Footprint-Free Cas9/CRISPR Editing of Cardiac-Associated Genes in Human Pluripotent Stem Cells. Stem Cells Dev. 2018;27:391-404 pubmed publisher
Chen X, Janssen J, Liu J, Maggio I, t Jong A, Mikkers H, et al. In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting. Nat Commun. 2017;8:657 pubmed publisher
Sosa E, Kim R, Rojas E, Hosohama L, Hennebold J, Orwig K, et al. An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC90) from embryonic fibroblasts. Stem Cell Res. 2017;21:5-8 pubmed publisher
Suchorska W, Augustyniak E, Richter M, Trzeciak T. Comparison of Four Protocols to Generate Chondrocyte-Like Cells from Human Induced Pluripotent Stem Cells (hiPSCs). Stem Cell Rev. 2017;13:299-308 pubmed publisher
Debowski K, Drummer C, Lentes J, Cors M, Dressel R, Lingner T, et al. The transcriptomes of novel marmoset monkey embryonic stem cell lines reflect distinct genomic features. Sci Rep. 2016;6:29122 pubmed publisher
Pei Y, Peng J, Behl M, Sipes N, Shockley K, Rao M, et al. Comparative neurotoxicity screening in human iPSC-derived neural stem cells, neurons and astrocytes. Brain Res. 2016;1638:57-73 pubmed publisher
Debowski K, Warthemann R, Lentes J, Salinas Riester G, Dressel R, Langenstroth D, et al. Non-viral generation of marmoset monkey iPS cells by a six-factor-in-one-vector approach. PLoS ONE. 2015;10:e0118424 pubmed publisher
Dianat N, Dubois Pot Schneider H, Steichen C, Desterke C, Leclerc P, Raveux A, et al. Generation of functional cholangiocyte-like cells from human pluripotent stem cells and HepaRG cells. Hepatology. 2014;60:700-14 pubmed publisher
Matsumoto Y, Hayashi Y, Schlieve C, Ikeya M, Kim H, Nguyen T, et al. Induced pluripotent stem cells from patients with human fibrodysplasia ossificans progressiva show increased mineralization and cartilage formation. Orphanet J Rare Dis. 2013;8:190 pubmed publisher
Choi W, Jeon H, Chung Y, Lim J, Shin D, Kim J, et al. Isolation and characterization of novel, highly proliferative human CD34/CD73-double-positive testis-derived stem cells for cell therapy. Stem Cells Dev. 2013;22:2158-73 pubmed publisher
Gkountela S, Li Z, Vincent J, Zhang K, Chen A, Pellegrini M, et al. The ontogeny of cKIT+ human primordial germ cells proves to be a resource for human germ line reprogramming, imprint erasure and in vitro differentiation. Nat Cell Biol. 2013;15:113-22 pubmed publisher
Chang C, Bouhassira E. Zinc-finger nuclease-mediated correction of ?-thalassemia in iPS cells. Blood. 2012;120:3906-14 pubmed publisher
Cayo M, Cai J, DeLaForest A, Noto F, Nagaoka M, Clark B, et al. JD induced pluripotent stem cell-derived hepatocytes faithfully recapitulate the pathophysiology of familial hypercholesterolemia. Hepatology. 2012;56:2163-71 pubmed publisher
Eildermann K, Gromoll J, Behr R. Misleading and reliable markers to differentiate between primate testis-derived multipotent stromal cells and spermatogonia in culture. Hum Reprod. 2012;27:1754-67 pubmed publisher
Chang C, Mitra K, Koya M, Velho M, Desprat R, Lenz J, et al. Production of embryonic and fetal-like red blood cells from human induced pluripotent stem cells. PLoS ONE. 2011;6:e25761 pubmed publisher
Dick E, Matsa E, Young L, Darling D, Denning C. Faster generation of hiPSCs by coupling high-titer lentivirus and column-based positive selection. Nat Protoc. 2011;6:701-14 pubmed publisher
Miranda Sayago J, Fernández Arcas N, Benito C, Reyes Engel A, Carrera J, Alonso A. Lifespan of human amniotic fluid-derived multipotent mesenchymal stromal cells. Cytotherapy. 2011;13:572-81 pubmed publisher
Macias M, Grande J, Moreno A, Dominguez I, Bornstein R, Flores A. Isolation and characterization of true mesenchymal stem cells derived from human term decidua capable of multilineage differentiation into all 3 embryonic layers. Am J Obstet Gynecol. 2010;203:495.e9-495.e23 pubmed publisher
Kamata M, Liang M, Liu S, Nagaoka Y, Chen I. Live cell monitoring of hiPSC generation and differentiation using differential expression of endogenous microRNAs. PLoS ONE. 2010;5:e11834 pubmed publisher
Kamata M, Liu S, Liang M, Nagaoka Y, Chen I. Generation of human induced pluripotent stem cells bearing an anti-HIV transgene by a lentiviral vector carrying an internal murine leukemia virus promoter. Hum Gene Ther. 2010;21:1555-67 pubmed publisher
Lee N, Kim J, Cho W, Lee M, Steiner R, Gompers A, et al. miR-302b maintains "stemness" of human embryonal carcinoma cells by post-transcriptional regulation of Cyclin D2 expression. Biochem Biophys Res Commun. 2008;377:434-440 pubmed publisher
Schopperle W, DeWolf W. The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma. Stem Cells. 2007;25:723-30 pubmed
Xu C, Inokuma M, Denham J, Golds K, Kundu P, Gold J, et al. Feeder-free growth of undifferentiated human embryonic stem cells. Nat Biotechnol. 2001;19:971-4 pubmed
Badcock G, Pigott C, Goepel J, Andrews P. The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan. Cancer Res. 1999;59:4715-9 pubmed
Andrews P, Banting G, Damjanov I, Arnaud D, Avner P. Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells. Hybridoma. 1984;3:347-61 pubmed
product information
Product Type :
Antibody
Product Name :
TRA-1-81 (Podocalyxin) Monoclonal Antibody (TRA-1-81), eBioscience™
Catalog # :
14-8883-82
Quantity :
100 µg
Price :
US 306
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Human
Applications :
Flow Cytometry: 0.5 µg/test, Immunocytochemistry: Assay-Dependent, Immunofluorescence: Assay-Dependent, Immunoprecipitation: Assay-Dependent, Western Blot: 5 µg/mL
Species :
Human
Clone :
TRA-1-81
Isotype :
IgM
Storage :
4° C
Description :
TRA-1-81 is a cell surface antigen expressed along with SSEA-3, SSEA-4 and TRA-1-60 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are down-regulated during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.5 µg/test, Immunocytochemistry: Assay-Dependent, Immunofluorescence: Assay-Dependent, Immunoprecipitation: Assay-Dependent, Western Blot: 5 µg/mL
Aliases :
TRA180; tra1-81
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA