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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
TRA-1-60 (Podocalyxin) Monoclonal Antibody (TRA-1-60), eBioscience™
catalog :
14-8863-82
quantity :
100 µg
price :
US 302
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
TRA-1-60
reactivity :
human, rhesus macaque
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
more info or order :
citations: 64
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; rhesus macaque; 1:100; fig 2c
Rodriguez Polo I, Mißbach S, Petkov S, Mattern F, Maierhofer A, Grządzielewska I, et al. A piggyBac-based platform for genome editing and clonal rhesus macaque iPSC line derivation. Sci Rep. 2021;11:15439 pubmed publisher
  • immunocytochemistry; human; 1:50; fig 1
Momcilovic O, Sivapatham R, Oron T, Meyer M, Mooney S, Rao M, et al. Derivation, Characterization, and Neural Differentiation of Integration-Free Induced Pluripotent Stem Cell Lines from Parkinson's Disease Patients Carrying SNCA, LRRK2, PARK2, and GBA Mutations. PLoS ONE. 2016;11:e0154890 pubmed publisher
  • immunocytochemistry; human
Sivapatham R, Zeng X. Generation and Characterization of Patient-Specific Induced Pluripotent Stem Cell for Disease Modeling. Methods Mol Biol. 2016;1353:25-44 pubmed publisher
  • flow cytometry; human
Busskamp V, Lewis N, Guye P, Ng A, Shipman S, Byrne S, et al. Rapid neurogenesis through transcriptional activation in human stem cells. Mol Syst Biol. 2014;10:760 pubmed publisher
  • flow cytometry; human; 1:100
Gkountela S, Li Z, Chin C, Lee S, Clark A. PRMT5 is required for human embryonic stem cell proliferation but not pluripotency. Stem Cell Rev. 2014;10:230-9 pubmed publisher
Catlett T, Onesto M, McCann A, Rempel S, Glass J, Franz D, et al. RHOA signaling defects result in impaired axon guidance in iPSC-derived neurons from patients with tuberous sclerosis complex. Nat Commun. 2021;12:2589 pubmed publisher
Hathy E, Szabó E, Vincze K, Haltrich I, Kiss E, Varga N, et al. Generation of multiple iPSC clones from a male schizophrenia patient carrying de novo mutations in genes KHSRP, LRRC7, and KIR2DL1, and his parents. Stem Cell Res. 2021;51:102140 pubmed publisher
Petkov S, Dressel R, Rodriguez Polo I, Behr R. Controlling the Switch from Neurogenesis to Pluripotency during Marmoset Monkey Somatic Cell Reprogramming with Self-Replicating mRNAs and Small Molecules. Cells. 2020;9: pubmed publisher
Kim M, Hwang S, Yoon J, Jeong Y, Kim E, Hyun S. Optimized Approaches for the Induction of Putative Canine Induced Pluripotent Stem Cells from Old Fibroblasts Using Synthetic RNAs. Animals (Basel). 2020;10: pubmed publisher
Kim K, Wu Y, Yoon J, Adachi K, Wu G, Velychko S, et al. Reprogramming competence of OCT factors is determined by transactivation domains. Sci Adv. 2020;6: pubmed publisher
Saito K, Iioka H, Maruyama S, Sumardika I, Sakaguchi M, Kondo E. PODXL1 promotes metastasis of the pancreatic ductal adenocarcinoma by activating the C5aR/C5a axis from the tumor microenvironment. Neoplasia. 2019;21:1121-1132 pubmed publisher
Nakanishi M, Mitchell R, Benoit Y, Orlando L, Reid J, Shimada K, et al. Human Pluripotency Is Initiated and Preserved by a Unique Subset of Founder Cells. Cell. 2019;177:910-924.e22 pubmed publisher
Mair B, Tomic J, Masud S, Tonge P, Weiss A, Usaj M, et al. Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies. Cell Rep. 2019;27:599-615.e12 pubmed publisher
Ebrahimi A, Sevinç K, Gürhan Sevinç G, Cribbs A, Philpott M, Uyulur F, et al. Bromodomain inhibition of the coactivators CBP/EP300 facilitate cellular reprogramming. Nat Chem Biol. 2019;15:519-528 pubmed publisher
Linker S, Urban L, Clark S, Chhatriwala M, Amatya S, McCarthy D, et al. Combined single-cell profiling of expression and DNA methylation reveals splicing regulation and heterogeneity. Genome Biol. 2019;20:30 pubmed publisher
Li J, Narayanan C, Bian J, Sambo D, Brickler T, Zhang W, et al. A transient DMSO treatment increases the differentiation potential of human pluripotent stem cells through the Rb family. PLoS ONE. 2018;13:e0208110 pubmed publisher
Lo Sardo V, Chubukov P, Ferguson W, Kumar A, Teng E, Duran M, et al. Unveiling the Role of the Most Impactful Cardiovascular Risk Locus through Haplotype Editing. Cell. 2018;175:1796-1810.e20 pubmed publisher
Kutsche L, Gysi D, Fallmann J, Lenk K, Petri R, Swiersy A, et al. Combined Experimental and System-Level Analyses Reveal the Complex Regulatory Network of miR-124 during Human Neurogenesis. Cell Syst. 2018;7:438-452.e8 pubmed publisher
Rosati J, Ferrari D, Altieri F, Tardivo S, Ricciolini C, Fusilli C, et al. Establishment of stable iPS-derived human neural stem cell lines suitable for cell therapies. Cell Death Dis. 2018;9:937 pubmed publisher
Karch C, Hernández D, Wang J, Marsh J, Hewitt A, Hsu S, et al. Human fibroblast and stem cell resource from the Dominantly Inherited Alzheimer Network. Alzheimers Res Ther. 2018;10:69 pubmed publisher
Shaltouki A, Hsieh C, Kim M, Wang X. Alpha-synuclein delays mitophagy and targeting Miro rescues neuron loss in Parkinson's models. Acta Neuropathol. 2018;136:607-620 pubmed publisher
Riesenberg S, Maricic T. Targeting repair pathways with small molecules increases precise genome editing in pluripotent stem cells. Nat Commun. 2018;9:2164 pubmed publisher
Ali M, Khan S, Vasanth S, Ahmed M, Chen R, Na C, et al. Generation and Proteome Profiling of PBMC-Originated, iPSC-Derived Corneal Endothelial Cells. Invest Ophthalmol Vis Sci. 2018;59:2437-2444 pubmed publisher
Pastor W, Liu W, Chen D, Ho J, Kim R, Hunt T, et al. TFAP2C regulates transcription in human naive pluripotency by opening enhancers. Nat Cell Biol. 2018;20:553-564 pubmed publisher
Thekkeparambil Chandrabose S, Sriram S, Subramanian S, Cheng S, Ong W, Rozen S, et al. Amenable epigenetic traits of dental pulp stem cells underlie high capability of xeno-free episomal reprogramming. Stem Cell Res Ther. 2018;9:68 pubmed publisher
Kogut I, McCarthy S, Pavlova M, Astling D, Chen X, Jakimenko A, et al. High-efficiency RNA-based reprogramming of human primary fibroblasts. Nat Commun. 2018;9:745 pubmed publisher
Victor M, Richner M, Olsen H, Lee S, Monteys A, Ma C, et al. Striatal neurons directly converted from Huntington's disease patient fibroblasts recapitulate age-associated disease phenotypes. Nat Neurosci. 2018;21:341-352 pubmed publisher
Melguizo Sanchís D, Xu Y, Taheem D, Yu M, Tilgner K, Bárta T, et al. iPSC modeling of severe aplastic anemia reveals impaired differentiation and telomere shortening in blood progenitors. Cell Death Dis. 2018;9:128 pubmed publisher
Oleksiewicz U, Gładych M, Raman A, Heyn H, Mereu E, Chlebanowska P, et al. TRIM28 and Interacting KRAB-ZNFs Control Self-Renewal of Human Pluripotent Stem Cells through Epigenetic Repression of Pro-differentiation Genes. Stem Cell Reports. 2017;9:2065-2080 pubmed publisher
Ho J, Cattoglio C, McSwiggen D, Tjian R, Fong Y. Regulation of DNA demethylation by the XPC DNA repair complex in somatic and pluripotent stem cells. Genes Dev. 2017;31:830-844 pubmed publisher
Allison R, Edgar J, Pearson G, Rizo T, Newton T, Günther S, et al. Defects in ER-endosome contacts impact lysosome function in hereditary spastic paraplegia. J Cell Biol. 2017;216:1337-1355 pubmed publisher
Saini J, Corneo B, Miller J, Kiehl T, Wang Q, Boles N, et al. Nicotinamide Ameliorates Disease Phenotypes in a Human iPSC Model of Age-Related Macular Degeneration. Cell Stem Cell. 2017;20:635-647.e7 pubmed publisher
Trevisan M, Desole G, Costanzi G, Lavezzo E, Palu G, Barzon L. Reprogramming Methods Do Not Affect Gene Expression Profile of Human Induced Pluripotent Stem Cells. Int J Mol Sci. 2017;18: pubmed publisher
Suchorska W, Augustyniak E, Richter M, Trzeciak T. Comparison of Four Protocols to Generate Chondrocyte-Like Cells from Human Induced Pluripotent Stem Cells (hiPSCs). Stem Cell Rev. 2017;13:299-308 pubmed publisher
Kempf H, Olmer R, Haase A, Franke A, Bolesani E, Schwanke K, et al. Bulk cell density and Wnt/TGFbeta signalling regulate mesendodermal patterning of human pluripotent stem cells. Nat Commun. 2016;7:13602 pubmed publisher
Quintanilla R, Asprer J, Sylakowski K, Lakshmipathy U. Kinetic Measurement and Real Time Visualization of Somatic Reprogramming. J Vis Exp. 2016;: pubmed publisher
Debowski K, Drummer C, Lentes J, Cors M, Dressel R, Lingner T, et al. The transcriptomes of novel marmoset monkey embryonic stem cell lines reflect distinct genomic features. Sci Rep. 2016;6:29122 pubmed publisher
Kita H, Okamoto K, Kushima R, Kawauchi A, Chano T. Dimethyl sulfoxide induces chemotherapeutic resistance in the treatment of testicular embryonal carcinomas. Oncol Lett. 2015;10:661-666 pubmed
Jouni M, Si Tayeb K, Es Salah Lamoureux Z, Latypova X, Champon B, Caillaud A, et al. Toward Personalized Medicine: Using Cardiomyocytes Differentiated From Urine-Derived Pluripotent Stem Cells to Recapitulate Electrophysiological Characteristics of Type 2 Long QT Syndrome. J Am Heart Assoc. 2015;4:e002159 pubmed publisher
Debowski K, Warthemann R, Lentes J, Salinas Riester G, Dressel R, Langenstroth D, et al. Non-viral generation of marmoset monkey iPS cells by a six-factor-in-one-vector approach. PLoS ONE. 2015;10:e0118424 pubmed publisher
Barone A, Säljö K, Benktander J, Blomqvist M, Mansson J, Johansson B, et al. Sialyl-lactotetra, a novel cell surface marker of undifferentiated human pluripotent stem cells. J Biol Chem. 2014;289:18846-59 pubmed publisher
Szkolnicka D, Farnworth S, Lucendo Villarin B, Storck C, Zhou W, Iredale J, et al. Accurate prediction of drug-induced liver injury using stem cell-derived populations. Stem Cells Transl Med. 2014;3:141-8 pubmed publisher
Matsumoto Y, Hayashi Y, Schlieve C, Ikeya M, Kim H, Nguyen T, et al. Induced pluripotent stem cells from patients with human fibrodysplasia ossificans progressiva show increased mineralization and cartilage formation. Orphanet J Rare Dis. 2013;8:190 pubmed publisher
Ackermann M, Lachmann N, Hartung S, Eggenschwiler R, Pfaff N, Happle C, et al. Promoter and lineage independent anti-silencing activity of the A2 ubiquitous chromatin opening element for optimized human pluripotent stem cell-based gene therapy. Biomaterials. 2014;35:1531-42 pubmed publisher
Kuhn L, Liu Y, Boyd N, Dennis J, Jiang X, Xin X, et al. Developmental-like bone regeneration by human embryonic stem cell-derived mesenchymal cells. Tissue Eng Part A. 2014;20:365-77 pubmed publisher
Han H, Irimia M, Ross P, Sung H, Alipanahi B, David L, et al. MBNL proteins repress ES-cell-specific alternative splicing and reprogramming. Nature. 2013;498:241-5 pubmed publisher
Choi W, Jeon H, Chung Y, Lim J, Shin D, Kim J, et al. Isolation and characterization of novel, highly proliferative human CD34/CD73-double-positive testis-derived stem cells for cell therapy. Stem Cells Dev. 2013;22:2158-73 pubmed publisher
Chang C, Bouhassira E. Zinc-finger nuclease-mediated correction of ?-thalassemia in iPS cells. Blood. 2012;120:3906-14 pubmed publisher
Polentes J, Jendelova P, Cailleret M, Braun H, Romanyuk N, Tropel P, et al. Human induced pluripotent stem cells improve stroke outcome and reduce secondary degeneration in the recipient brain. Cell Transplant. 2012;21:2587-602 pubmed publisher
Cayo M, Cai J, DeLaForest A, Noto F, Nagaoka M, Clark B, et al. JD induced pluripotent stem cell-derived hepatocytes faithfully recapitulate the pathophysiology of familial hypercholesterolemia. Hepatology. 2012;56:2163-71 pubmed publisher
Onder T, Kara N, Cherry A, Sinha A, Zhu N, Bernt K, et al. Chromatin-modifying enzymes as modulators of reprogramming. Nature. 2012;483:598-602 pubmed publisher
Chang C, Mitra K, Koya M, Velho M, Desprat R, Lenz J, et al. Production of embryonic and fetal-like red blood cells from human induced pluripotent stem cells. PLoS ONE. 2011;6:e25761 pubmed publisher
Kuo T, Chen C, Baron D, Onder T, Loewer S, Almeida S, et al. Midbody accumulation through evasion of autophagy contributes to cellular reprogramming and tumorigenicity. Nat Cell Biol. 2011;13:1214-23 pubmed publisher
Wada N, Wang B, Lin N, Laslett A, Gronthos S, Bartold P. Induced pluripotent stem cell lines derived from human gingival fibroblasts and periodontal ligament fibroblasts. J Periodontal Res. 2011;46:438-47 pubmed publisher
Miranda Sayago J, Fernández Arcas N, Benito C, Reyes Engel A, Carrera J, Alonso A. Lifespan of human amniotic fluid-derived multipotent mesenchymal stromal cells. Cytotherapy. 2011;13:572-81 pubmed publisher
Loewer S, Cabili M, Guttman M, Loh Y, Thomas K, Park I, et al. Large intergenic non-coding RNA-RoR modulates reprogramming of human induced pluripotent stem cells. Nat Genet. 2010;42:1113-7 pubmed publisher
Macias M, Grande J, Moreno A, Dominguez I, Bornstein R, Flores A. Isolation and characterization of true mesenchymal stem cells derived from human term decidua capable of multilineage differentiation into all 3 embryonic layers. Am J Obstet Gynecol. 2010;203:495.e9-495.e23 pubmed publisher
Kamata M, Liang M, Liu S, Nagaoka Y, Chen I. Live cell monitoring of hiPSC generation and differentiation using differential expression of endogenous microRNAs. PLoS ONE. 2010;5:e11834 pubmed publisher
Kamata M, Liu S, Liang M, Nagaoka Y, Chen I. Generation of human induced pluripotent stem cells bearing an anti-HIV transgene by a lentiviral vector carrying an internal murine leukemia virus promoter. Hum Gene Ther. 2010;21:1555-67 pubmed publisher
Kozubenko N, Turnovcova K, Kapcalova M, Butenko O, Anderova M, Rusnakova V, et al. Analysis of in vitro and in vivo characteristics of human embryonic stem cell-derived neural precursors. Cell Transplant. 2010;19:471-86 pubmed publisher
Chin A, Fong W, Goh L, Philp R, Oh S, Choo A. Identification of proteins from feeder conditioned medium that support human embryonic stem cells. J Biotechnol. 2007;130:320-8 pubmed
Schopperle W, DeWolf W. The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma. Stem Cells. 2007;25:723-30 pubmed
Badcock G, Pigott C, Goepel J, Andrews P. The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan. Cancer Res. 1999;59:4715-9 pubmed
Andrews P, Banting G, Damjanov I, Arnaud D, Avner P. Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells. Hybridoma. 1984;3:347-61 pubmed
product information
Product Type :
Antibody
Product Name :
TRA-1-60 (Podocalyxin) Monoclonal Antibody (TRA-1-60), eBioscience™
Catalog # :
14-8863-82
Quantity :
100 µg
Price :
US 302
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Human
Applications :
Flow Cytometry: 0.5 µg/test, Immunohistochemistry: Assay-Dependent, Immunoprecipitation: Assay-Dependent, Western Blot: Assay-Dependent
Species :
Human
Clone :
TRA-1-60
Isotype :
IgM
Storage :
4° C
Description :
TRA-1-60 is a cell surface antigen, expressed along with SSEA-3, SSEA-4 and TRA-1-81 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are lost during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.5 µg/test, Immunohistochemistry: Assay-Dependent, Immunoprecipitation: Assay-Dependent, Western Blot: Assay-Dependent
Aliases :
TRA160; tra60
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA