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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Granzyme B Monoclonal Antibody (16G6), eBioscience™
catalog :
14-8822-82
quantity :
100 µg
price :
US 332
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
16G6
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
citations: 68
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 2c
Mangan M, Bird C, Kaiserman D, Matthews A, Hitchen C, Steer D, et al. A Novel Serpin Regulatory Mechanism: SerpinB9 IS REVERSIBLY INHIBITED BY VICINAL DISULFIDE BOND FORMATION IN THE REACTIVE CENTER LOOP. J Biol Chem. 2016;291:3626-38 pubmed publisher
  • flow cytometry; mouse
Pearce V, Bouabe H, MacQueen A, Carbonaro V, Okkenhaug K. PI3Kδ Regulates the Magnitude of CD8+ T Cell Responses after Challenge with Listeria monocytogenes. J Immunol. 2015;195:3206-17 pubmed publisher
  • flow cytometry; mouse
Adoro S, McCaughtry T, Erman B, Alag A, Van Laethem F, Park J, et al. Coreceptor gene imprinting governs thymocyte lineage fate. EMBO J. 2012;31:366-77 pubmed publisher
  • flow cytometry; mouse; fig 2, 3
Lin P, Sun L, Thibodeaux S, Ludwig S, Vadlamudi R, Hurez V, et al. B7-H1-dependent sex-related differences in tumor immunity and immunotherapy responses. J Immunol. 2010;185:2747-53 pubmed publisher
  • flow cytometry; mouse; fig 2e
Waugh C, Sinclair L, Finlay D, Bayascas J, Cantrell D. Phosphoinositide (3,4,5)-triphosphate binding to phosphoinositide-dependent kinase 1 regulates a protein kinase B/Akt signaling threshold that dictates T-cell migration, not proliferation. Mol Cell Biol. 2009;29:5952-62 pubmed publisher
  • flow cytometry; mouse; fig 4
Ahonen C, Wasiuk A, Fuse S, Turk M, Ernstoff M, Suriawinata A, et al. Enhanced efficacy and reduced toxicity of multifactorial adjuvants compared with unitary adjuvants as cancer vaccines. Blood. 2008;111:3116-25 pubmed publisher
  • flow cytometry; mouse
Hickman H, Takeda K, Skon C, Murray F, Hensley S, Loomis J, et al. Direct priming of antiviral CD8+ T cells in the peripheral interfollicular region of lymph nodes. Nat Immunol. 2008;9:155-65 pubmed publisher
Jiang L, Wang Y, Zhao J, Uehara M, Hou Q, Kasinath V, et al. Direct Tumor Killing and Immunotherapy through Anti-SerpinB9 Therapy. Cell. 2020;183:1219-1233.e18 pubmed publisher
Luu M, Weigand K, Wedi F, Breidenbend C, Leister H, Pautz S, et al. Regulation of the effector function of CD8+ T cells by gut microbiota-derived metabolite butyrate. Sci Rep. 2018;8:14430 pubmed publisher
Wang J, Xie T, Wang B, William W, Heymach J, El Naggar A, et al. PD-1 Blockade Prevents the Development and Progression of Carcinogen-Induced Oral Premalignant Lesions. Cancer Prev Res (Phila). 2017;10:684-693 pubmed publisher
Konishi M, Erdem S, Weissleder R, Lichtman A, McCarthy J, Libby P. Imaging Granzyme B Activity Assesses Immune-Mediated Myocarditis. Circ Res. 2015;117:502-512 pubmed publisher
Andoniou C, Sutton V, Wikstrom M, Fleming P, Thia K, Matthews A, et al. A natural genetic variant of granzyme B confers lethality to a common viral infection. PLoS Pathog. 2014;10:e1004526 pubmed publisher
Boulet S, Daudelin J, Labrecque N. IL-2 induction of Blimp-1 is a key in vivo signal for CD8+ short-lived effector T cell differentiation. J Immunol. 2014;193:1847-54 pubmed publisher
Bird C, Christensen M, Mangan M, Prakash M, Sedelies K, Smyth M, et al. The granzyme B-Serpinb9 axis controls the fate of lymphocytes after lysosomal stress. Cell Death Differ. 2014;21:876-87 pubmed publisher
Peng H, Sun R, Tang L, Wei H, Tian Z. CD62L is critical for maturation and accumulation of murine hepatic NK cells in response to viral infection. J Immunol. 2013;190:4255-62 pubmed publisher
Daudelin J, Mathieu M, Boulet S, Labrecque N. IL-6 production by dendritic cells is dispensable for CD8+ memory T-cell generation. Biomed Res Int. 2013;2013:126189 pubmed publisher
Visekruna A, Ritter J, Scholz T, Campos L, Guralnik A, Poncette L, et al. Tc9 cells, a new subset of CD8(+) T cells, support Th2-mediated airway inflammation. Eur J Immunol. 2013;43:606-18 pubmed publisher
Choi J, Ziga E, Ritchey J, Collins L, Prior J, Cooper M, et al. IFN?R signaling mediates alloreactive T-cell trafficking and GVHD. Blood. 2012;120:4093-103 pubmed publisher
Lemay C, Rintoul J, Kus A, Paterson J, Garcia V, Falls T, et al. Harnessing oncolytic virus-mediated antitumor immunity in an infected cell vaccine. Mol Ther. 2012;20:1791-9 pubmed publisher
Kaul V, Van Kaer L, Das G, Das J. Prostanoid receptor 2 signaling protects T helper 2 cells from BALB/c mice against activation-induced cell death. J Biol Chem. 2012;287:25434-9 pubmed publisher
Bao Y, Zheng J, Han C, Jin J, Han H, Liu Y, et al. Tyrosine kinase Btk is required for NK cell activation. J Biol Chem. 2012;287:23769-78 pubmed publisher
Wu L, Yun Z, Tagawa T, Rey McIntyre K, de Perrot M. CTLA-4 blockade expands infiltrating T cells and inhibits cancer cell repopulation during the intervals of chemotherapy in murine mesothelioma. Mol Cancer Ther. 2012;11:1809-19 pubmed publisher
Li Q, Rao R, Vazzana J, Goedegebuure P, Odunsi K, Gillanders W, et al. Regulating mammalian target of rapamycin to tune vaccination-induced CD8(+) T cell responses for tumor immunity. J Immunol. 2012;188:3080-7 pubmed publisher
Mathieu M, Cotta Grand N, Daudelin J, Boulet S, Lapointe R, Labrecque N. CD40-activated B cells can efficiently prime antigen-specific naïve CD8+ T cells to generate effector but not memory T cells. PLoS ONE. 2012;7:e30139 pubmed publisher
Bird C, Hitchen C, Prescott M, Harper I, Bird P. Immunodetection of granzyme B tissue distribution and cellular localisation. Methods Mol Biol. 2012;844:237-50 pubmed publisher
Drobits B, Holcmann M, Amberg N, Swiecki M, Grundtner R, Hammer M, et al. Imiquimod clears tumors in mice independent of adaptive immunity by converting pDCs into tumor-killing effector cells. J Clin Invest. 2012;122:575-85 pubmed publisher
Wu X, Chen Y, Wei H, Sun R, Tian Z. Development of murine hepatic NK cells during ontogeny: comparison with spleen NK cells. Clin Dev Immunol. 2012;2012:759765 pubmed publisher
Wiesel M, Crouse J, Bedenikovic G, Sutherland A, Joller N, Oxenius A. Type-I IFN drives the differentiation of short-lived effector CD8+ T cells in vivo. Eur J Immunol. 2012;42:320-9 pubmed publisher
Gonzaga R, Matzinger P, Perez Diez A. Resident peritoneal NK cells. J Immunol. 2011;187:6235-42 pubmed publisher
Schmidt R, Filak H, Lemon J, Potter T, Lenz L. A LysM and SH3-domain containing region of the Listeria monocytogenes p60 protein stimulates accessory cells to promote activation of host NK cells. PLoS Pathog. 2011;7:e1002368 pubmed publisher
Reddy P, Sehrawat S, Suryawanshi A, Rajasagi N, Mulik S, Hirashima M, et al. Influence of galectin-9/Tim-3 interaction on herpes simplex virus-1 latency. J Immunol. 2011;187:5745-55 pubmed publisher
Zappasodi R, Bongarzone I, Ghedini G, Castagnoli L, Cabras A, Messina A, et al. Serological identification of HSP105 as a novel non-Hodgkin lymphoma therapeutic target. Blood. 2011;118:4421-30 pubmed publisher
Yu Y, Wang D, Liu C, Kaosaard K, Semple K, Anasetti C, et al. Prevention of GVHD while sparing GVL effect by targeting Th1 and Th17 transcription factor T-bet and ROR?t in mice. Blood. 2011;118:5011-20 pubmed publisher
Qu Y, Taylor J, Bose A, Storkus W. Therapeutic effectiveness of intratumorally delivered dendritic cells engineered to express the pro-inflammatory cytokine, interleukin (IL)-32. Cancer Gene Ther. 2011;18:663-73 pubmed publisher
Thayer T, Delano M, Liu C, Chen J, Padgett L, Tse H, et al. Superoxide production by macrophages and T cells is critical for the induction of autoreactivity and type 1 diabetes. Diabetes. 2011;60:2144-51 pubmed publisher
Tan X, Sande J, Pufnock J, Blattman J, Greenberg P. Retinoic acid as a vaccine adjuvant enhances CD8+ T cell response and mucosal protection from viral challenge. J Virol. 2011;85:8316-27 pubmed publisher
Kitajima M, Ito T, Tumes D, Endo Y, Onodera A, Hashimoto K, et al. Memory type 2 helper T cells induce long-lasting antitumor immunity by activating natural killer cells. Cancer Res. 2011;71:4790-8 pubmed publisher
Tabbekh M, Franciszkiewicz K, Haouas H, Lecluse Y, Benihoud K, Raman C, et al. Rescue of tumor-infiltrating lymphocytes from activation-induced cell death enhances the antitumor CTL response in CD5-deficient mice. J Immunol. 2011;187:102-9 pubmed publisher
Tosiek M, Gruber A, Bader S, Mauel S, Hoymann H, Prettin S, et al. CD4+CD25+Foxp3+ regulatory T cells are dispensable for controlling CD8+ T cell-mediated lung inflammation. J Immunol. 2011;186:6106-18 pubmed publisher
Hwang W, Jung K, Jeon Y, Yun S, Kim T, Choi I. Knockdown of the interleukin-6 receptor alpha chain of dendritic cell vaccines enhances the therapeutic potential against IL-6 producing tumors. Vaccine. 2010;29:34-44 pubmed publisher
Ghaemi A, Soleimanjahi H, Gill P, Hassan Z, Razeghi S, Fazeli M, et al. Protection of mice by a ?-based therapeutic vaccine against cancer associated with human papillomavirus type 16. Intervirology. 2011;54:105-12 pubmed publisher
McCarl C, Khalil S, Ma J, Oh Hora M, Yamashita M, Roether J, et al. Store-operated Ca2+ entry through ORAI1 is critical for T cell-mediated autoimmunity and allograft rejection. J Immunol. 2010;185:5845-58 pubmed publisher
Zhang S, Rozell M, Verma R, Albu D, Califano D, VanValkenburgh J, et al. Antigen-specific clonal expansion and cytolytic effector function of CD8+ T lymphocytes depend on the transcription factor Bcl11b. J Exp Med. 2010;207:1687-99 pubmed publisher
Stevenson H, Estes M, Thirumalapura N, Walker D, Ismail N. Natural killer cells promote tissue injury and systemic inflammatory responses during fatal Ehrlichia-induced toxic shock-like syndrome. Am J Pathol. 2010;177:766-76 pubmed publisher
Hillhouse E, Beauchamp C, Chabot Roy G, Dugas V, Lesage S. Interleukin-10 limits the expansion of immunoregulatory CD4-CD8- T cells in autoimmune-prone non-obese diabetic mice. Immunol Cell Biol. 2010;88:771-80 pubmed publisher
Søndergaard H, Galsgaard E, Bartholomaeussen M, Straten P, Odum N, Skak K. Intratumoral interleukin-21 increases antitumor immunity, tumor-infiltrating CD8+ T-cell density and activity, and enlarges draining lymph nodes. J Immunother. 2010;33:236-49 pubmed publisher
Martinez J, Huang X, Yang Y. Direct TLR2 signaling is critical for NK cell activation and function in response to vaccinia viral infection. PLoS Pathog. 2010;6:e1000811 pubmed publisher
Haeberlein S, Sebald H, Bogdan C, Schleicher U. IL-18, but not IL-15, contributes to the IL-12-dependent induction of NK-cell effector functions by Leishmania infantum in vivo. Eur J Immunol. 2010;40:1708-17 pubmed publisher
Xie Y, Akpinarli A, Maris C, Hipkiss E, Lane M, Kwon E, et al. Naive tumor-specific CD4(+) T cells differentiated in vivo eradicate established melanoma. J Exp Med. 2010;207:651-67 pubmed publisher
Kenna T, Waldie T, McNally A, Thomson M, Yagita H, Thomas R, et al. Targeting antigen to diverse APCs inactivates memory CD8+ T cells without eliciting tissue-destructive effector function. J Immunol. 2010;184:598-606 pubmed publisher
Tabayoyong W, Salas J, Bonde S, Zavazava N. HOXB4-transduced embryonic stem cell-derived Lin-c-kit+ and Lin-Sca-1+ hematopoietic progenitors express H60 and are targeted by NK cells. J Immunol. 2009;183:5449-57 pubmed publisher
Zhao J, Zhao J, Perlman S. De novo recruitment of antigen-experienced and naive T cells contributes to the long-term maintenance of antiviral T cell populations in the persistently infected central nervous system. J Immunol. 2009;183:5163-70 pubmed publisher
Thapa M, Carr D. CXCR3 deficiency increases susceptibility to genital herpes simplex virus type 2 infection: Uncoupling of CD8+ T-cell effector function but not migration. J Virol. 2009;83:9486-501 pubmed publisher
Huber M, Heink S, Grothe H, Guralnik A, Reinhard K, Elflein K, et al. A Th17-like developmental process leads to CD8(+) Tc17 cells with reduced cytotoxic activity. Eur J Immunol. 2009;39:1716-25 pubmed publisher
Itsumi M, Yoshikai Y, Yamada H. IL-15 is critical for the maintenance and innate functions of self-specific CD8(+) T cells. Eur J Immunol. 2009;39:1784-93 pubmed publisher
Lin Y, Chang L, Huang C, Peng H, Dutta A, Chen T, et al. Effector/memory but not naive regulatory T cells are responsible for the loss of concomitant tumor immunity. J Immunol. 2009;182:6095-104 pubmed publisher
Rajasagi N, Kassim S, Kollias C, Zhao X, Chervenak R, Jennings S. CD4+ T cells are required for the priming of CD8+ T cells following infection with herpes simplex virus type 1. J Virol. 2009;83:5256-68 pubmed publisher
Archambaud C, Sansoni A, Mingueneau M, Devilard E, Delsol G, Malissen B, et al. STAT6 deletion converts the Th2 inflammatory pathology afflicting Lat(Y136F) mice into a lymphoproliferative disorder involving Th1 and CD8 effector T cells. J Immunol. 2009;182:2680-9 pubmed publisher
Fujita M, Zhu X, Ueda R, Sasaki K, Kohanbash G, Kastenhuber E, et al. Effective immunotherapy against murine gliomas using type 1 polarizing dendritic cells--significant roles of CXCL10. Cancer Res. 2009;69:1587-95 pubmed publisher
Freishtat R, Natale J, Benton A, Cohen J, Sharron M, Wiles A, et al. Sepsis alters the megakaryocyte-platelet transcriptional axis resulting in granzyme B-mediated lymphotoxicity. Am J Respir Crit Care Med. 2009;179:467-73 pubmed publisher
Nagahara K, Arikawa T, Oomizu S, Kontani K, Nobumoto A, Tateno H, et al. Galectin-9 increases Tim-3+ dendritic cells and CD8+ T cells and enhances antitumor immunity via galectin-9-Tim-3 interactions. J Immunol. 2008;181:7660-9 pubmed
Calbo S, Delagrèverie H, Arnoult C, Authier F, Tron F, Boyer O. Functional tolerance of CD8+ T cells induced by muscle-specific antigen expression. J Immunol. 2008;181:408-17 pubmed
Jin G, Hirano T, Murakami M. Combination treatment with IL-2 and anti-IL-2 mAbs reduces tumor metastasis via NK cell activation. Int Immunol. 2008;20:783-9 pubmed publisher
Hutchens M, Luker K, Sottile P, Sonstein J, Lukacs N, Nunez G, et al. TLR3 increases disease morbidity and mortality from vaccinia infection. J Immunol. 2008;180:483-91 pubmed
Liu S, Lizee G, Lou Y, Liu C, Overwijk W, Wang G, et al. IL-21 synergizes with IL-7 to augment expansion and anti-tumor function of cytotoxic T cells. Int Immunol. 2007;19:1213-21 pubmed
Arrode G, Hegde R, Mani A, Jin Y, Chebloune Y, Narayan O. Phenotypic and functional analysis of immune CD8+ T cell responses induced by a single injection of a HIV DNA vaccine in mice. J Immunol. 2007;178:2318-27 pubmed
Erman B, Alag A, Dahle O, Van Laethem F, Sarafova S, Guinter T, et al. Coreceptor signal strength regulates positive selection but does not determine CD4/CD8 lineage choice in a physiologic in vivo model. J Immunol. 2006;177:6613-25 pubmed
Sadighi Akha A, Berger S, Miller R. Enhancement of CD8 T-cell function through modifying surface glycoproteins in young and old mice. Immunology. 2006;119:187-94 pubmed
product information
Product Type :
Antibody
Product Name :
Granzyme B Monoclonal Antibody (16G6), eBioscience™
Catalog # :
14-8822-82
Quantity :
100 µg
Price :
US 332
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
ELISA: 1-4 µg/mL, Immunohistochemistry (Paraffin): Assay-Dependent, Western Blot: 1 µg/mL
Species :
Mouse
Clone :
16G6
Isotype :
IgG2b, kappa
Storage :
4° C
Description :
Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells. Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.
Format :
Liquid
Applications w/Dilutions :
ELISA: 1-4 µg/mL, Immunohistochemistry (Paraffin): Assay-Dependent, Western Blot: 1 µg/mL
Aliases :
AI553453; C11; cathepsin G-like 1; CCP1; CCP-1/C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; CTLA1; CTLA-1; CTSGL1; Cytotoxic cell protease 1; cytotoxic serine protease B; cytotoxic T-lymphocyte proteinase 2; cytotoxic T-lymphocyte-associated serine esterase 1; fragmentin; fragmentin 2; fragmentin-2; GLP I; GLP III; GLP-1; granzyme 2; granzyme B; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); granzyme B(G,H); Granzyme-2; GranzymeB; granzyme-like protein 1; granzyme-like protein I; GRB; GZB; GZMB; HLP; Human lymphocyte protein; Lymphocyte protease; Natural killer cell protease 1; OTTHUMP00000028189; RNKP-1; SECT; T-cell serine protease 1-3E
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA