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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
FOXP3 Monoclonal Antibody (eBio7979 (221D/D3)), eBioscience™
catalog :
14-7979-80
quantity :
25 µg
price :
US 169
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
eBio7979 (221D/D3)
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunohistochemistry - paraffin section
more info or order :
citations: 50
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; human; loading ...; fig 5b
Qu S, Xue H, Dong X, Lin D, Wu R, Nabavi N, et al. Aneustat (OMN54) has aerobic glycolysis-inhibitory activity and also immunomodulatory activity as indicated by a first-generation PDX prostate cancer model. Int J Cancer. 2018;143:419-429 pubmed publisher
  • western blot; mouse; loading ...; fig 1d
Mailer R, Gisterå A, Polyzos K, Ketelhuth D, Hansson G. Hypercholesterolemia Enhances T Cell Receptor Signaling and Increases the Regulatory T Cell Population. Sci Rep. 2017;7:15655 pubmed publisher
  • western blot; human; loading ...; fig 1a
Jou Y, Tsai Y, Lin C, Tung C, Shen C, Tsai H, et al. Foxp3 enhances HIF-1α target gene expression in human bladder cancer through decreasing its ubiquitin-proteasomal degradation. Oncotarget. 2016;7:65403-65417 pubmed publisher
  • western blot; mouse; 1:500; fig 5,6
Hsiao H, Hsu T, Liu W, Hsieh W, Chou T, Wu Y, et al. Deltex1 antagonizes HIF-1α and sustains the stability of regulatory T cells in vivo. Nat Commun. 2015;6:6353 pubmed publisher
  • western blot; mouse
Rutz S, Kayagaki N, Phung Q, Eidenschenk C, Noubade R, Wang X, et al. Deubiquitinase DUBA is a post-translational brake on interleukin-17 production in T cells. Nature. 2015;518:417-21 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:50; tbl 2
West N, Panet Raymond V, Truong P, Alexander C, Babinszky S, Milne K, et al. Intratumoral Immune Responses Can Distinguish New Primary and True Recurrence Types of Ipsilateral Breast Tumor Recurrences (IBTR). Breast Cancer (Auckl). 2011;5:105-15 pubmed publisher
  • western blot; mouse; 2 ug/ml; fig 7
Ellestad K, Tsutsui S, Noorbakhsh F, Warren K, Yong V, Pittman Q, et al. Early life exposure to lipopolysaccharide suppresses experimental autoimmune encephalomyelitis by promoting tolerogenic dendritic cells and regulatory T cells. J Immunol. 2009;183:298-309 pubmed publisher
  • immunohistochemistry; human; 1:50; fig 2
Milne K, Barnes R, Girardin A, Mawer M, Nesslinger N, Ng A, et al. Tumor-infiltrating T cells correlate with NY-ESO-1-specific autoantibodies in ovarian cancer. PLoS ONE. 2008;3:e3409 pubmed publisher
Wang A, Yang M, Liang R, Zhu F, Zhu F, Liu X, et al. Mouse Double Minute 2 Homolog-Mediated Ubiquitination Facilitates Forkhead Box P3 Stability and Positively Modulates Human Regulatory T Cell Function. Front Immunol. 2020;11:1087 pubmed publisher
Pi Castro D, José López R, Fernández Flores F, Rabanal Prados R, Mandara M, Arus C, et al. Expression of FOXP3 in Canine Gliomas: Immunohistochemical Study of Tumor-Infiltrating Regulatory Lymphocytes. J Neuropathol Exp Neurol. 2020;79:184-193 pubmed publisher
Xie C, Zhu F, Wang J, Zhang W, Bellanti J, Li B, et al. Off-Target Deletion of Conditional Dbc1 Allele in the Foxp3YFP-Cre Mouse Line under Specific Setting. Cells. 2019;8: pubmed publisher
Sun R, Yang Y, Gu Z, Tang X, Zhang C, Kou W, et al. Silencing of CD86 in dendritic cells by small interfering RNA regulates cytokine production in T cells from patients with allergic rhinitis in vitro. Mol Med Rep. 2019;20:3893-3900 pubmed publisher
Yu Y, Ma X, Zhang Y, Zhang Y, Ying J, Zhang W, et al. Changes in Expression of Multiple Checkpoint Molecules and Infiltration of Tumor Immune Cells after Neoadjuvant Chemotherapy in Gastric Cancer. J Cancer. 2019;10:2754-2763 pubmed publisher
Zhu F, Yi G, Liu X, Zhu F, Zhao A, Wang A, et al. Ring finger protein 31-mediated atypical ubiquitination stabilizes forkhead box P3 and thereby stimulates regulatory T-cell function. J Biol Chem. 2018;293:20099-20111 pubmed publisher
Schmidt A, Marabita F, Kiani N, Gross C, Johansson H, Elias S, et al. Time-resolved transcriptome and proteome landscape of human regulatory T cell (Treg) differentiation reveals novel regulators of FOXP3. BMC Biol. 2018;16:47 pubmed publisher
Lu Y, Kim N, Jiang Y, Zhang H, Zheng D, Zhu F, et al. Cambogin suppresses dextran sulphate sodium-induced colitis by enhancing Treg cell stability and function. Br J Pharmacol. 2018;175:1085-1099 pubmed publisher
Jiao J, Han R, Hancock W, Beier U. Proximity Ligation Assay to Quantify Foxp3 Acetylation in Regulatory T Cells. Methods Mol Biol. 2017;1510:287-293 pubmed
Kuczma M, Wang C, Ignatowicz L, GOURDIE R, Kraj P. Altered connexin 43 expression underlies age-dependent decrease of regulatory T cell suppressor function in nonobese diabetic mice. J Immunol. 2015;194:5261-71 pubmed publisher
Kuypers E, Willems M, Jellema R, Kemp M, Newnham J, Delhaas T, et al. Responses of the spleen to intraamniotic lipopolysaccharide exposure in fetal sheep. Pediatr Res. 2015;77:29-35 pubmed publisher
Zhang Y, Yan W, Mathew E, Bednar F, Wan S, Collins M, et al. CD4+ T lymphocyte ablation prevents pancreatic carcinogenesis in mice. Cancer Immunol Res. 2014;2:423-35 pubmed publisher
Wolfs T, Kramer B, Thuijls G, Kemp M, Saito M, Willems M, et al. Chorioamnionitis-induced fetal gut injury is mediated by direct gut exposure of inflammatory mediators or by lung inflammation. Am J Physiol Gastrointest Liver Physiol. 2014;306:G382-93 pubmed publisher
Townsend K, Spowart J, Huwait H, Eshragh S, West N, Elrick M, et al. Markers of T cell infiltration and function associate with favorable outcome in vascularized high-grade serous ovarian carcinoma. PLoS ONE. 2013;8:e82406 pubmed publisher
Rojo J, Ojeda G, Acosta Y, Montes Casado M, Criado G, Portoles P. Characteristics of TCR/CD3 complex CD3{varepsilon} chains of regulatory CD4+ T (Treg) lymphocytes: role in Treg differentiation in vitro and impact on Treg in vivo. J Leukoc Biol. 2014;95:441-50 pubmed publisher
Donius L, Handy J, Weis J, Weis J. Optimal germinal center B cell activation and T-dependent antibody responses require expression of the mouse complement receptor Cr1. J Immunol. 2013;191:434-47 pubmed publisher
Kemp M, Kannan P, Saito M, Newnham J, Cox T, Jobe A, et al. Selective exposure of the fetal lung and skin/amnion (but not gastro-intestinal tract) to LPS elicits acute systemic inflammation in fetal sheep. PLoS ONE. 2013;8:e63355 pubmed publisher
Kuypers E, Wolfs T, Collins J, Jellema R, Newnham J, Kemp M, et al. Intraamniotic lipopolysaccharide exposure changes cell populations and structure of the ovine fetal thymus. Reprod Sci. 2013;20:946-56 pubmed publisher
Kim J, Chon S, Im K, Kim N, Cho K, Sur J. Infiltrating Foxp3+ regulatory T cells and histopathological features in canine classical and spermatocytic seminomas. Reprod Domest Anim. 2013;48:218-22 pubmed publisher
Kuypers E, Collins J, Jellema R, Wolfs T, Kemp M, Nitsos I, et al. Ovine fetal thymus response to lipopolysaccharide-induced chorioamnionitis and antenatal corticosteroids. PLoS ONE. 2012;7:e38257 pubmed publisher
Kuypers E, Collins J, Kramer B, Ofman G, Nitsos I, Pillow J, et al. Intra-amniotic LPS and antenatal betamethasone: inflammation and maturation in preterm lamb lungs. Am J Physiol Lung Cell Mol Physiol. 2012;302:L380-9 pubmed publisher
Katoh H, Qin Z, Liu R, Wang L, Li W, Li X, et al. FOXP3 orchestrates H4K16 acetylation and H3K4 trimethylation for activation of multiple genes by recruiting MOF and causing displacement of PLU-1. Mol Cell. 2011;44:770-84 pubmed publisher
Kim J, Hur J, Lee S, Im K, Kim N, Sur J. Correlation of Foxp3 positive regulatory T cells with prognostic factors in canine mammary carcinomas. Vet J. 2012;193:222-7 pubmed publisher
Beyer M, Thabet Y, Muller R, Sadlon T, Classen S, Lahl K, et al. Repression of the genome organizer SATB1 in regulatory T cells is required for suppressive function and inhibition of effector differentiation. Nat Immunol. 2011;12:898-907 pubmed publisher
Kallapur S, Kramer B, Nitsos I, Pillow J, Collins J, Polglase G, et al. Pulmonary and systemic inflammatory responses to intra-amniotic IL-1? in fetal sheep. Am J Physiol Lung Cell Mol Physiol. 2011;301:L285-95 pubmed publisher
Kuczma M, Lee J, Kraj P. Connexin 43 signaling enhances the generation of Foxp3+ regulatory T cells. J Immunol. 2011;187:248-57 pubmed publisher
Häusler S, Montalbán Del Barrio I, Strohschein J, Chandran P, Engel J, Honig A, et al. Ectonucleotidases CD39 and CD73 on OvCA cells are potent adenosine-generating enzymes responsible for adenosine receptor 2A-dependent suppression of T cell function and NK cell cytotoxicity. Cancer Immunol Immunother. 2011;60:1405-18 pubmed publisher
Zhang L, Ma J, Takeuchi M, Usui Y, Hattori T, Okunuki Y, et al. Suppression of experimental autoimmune uveoretinitis by inducing differentiation of regulatory T cells via activation of aryl hydrocarbon receptor. Invest Ophthalmol Vis Sci. 2010;51:2109-17 pubmed publisher
Mas E, Danjoux M, Garcia V, Carpentier S, Segui B, Levade T. IL-6 deficiency attenuates murine diet-induced non-alcoholic steatohepatitis. PLoS ONE. 2009;4:e7929 pubmed publisher
Kuczma M, Podolsky R, Garge N, Daniely D, Pacholczyk R, Ignatowicz L, et al. Foxp3-deficient regulatory T cells do not revert into conventional effector CD4+ T cells but constitute a unique cell subset. J Immunol. 2009;183:3731-41 pubmed publisher
Kuczma M, Pawlikowska I, Kopij M, Podolsky R, Rempala G, Kraj P. TCR repertoire and Foxp3 expression define functionally distinct subsets of CD4+ regulatory T cells. J Immunol. 2009;183:3118-29 pubmed publisher
Cao Z, Wara A, Icli B, Sun X, Packard R, Esen F, et al. Kruppel-like factor KLF10 targets transforming growth factor-beta1 to regulate CD4(+)CD25(-) T cells and T regulatory cells. J Biol Chem. 2009;284:24914-24 pubmed publisher
Lybeck K, Storset A, Olsen I. Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium avium subsp. paratuberculosis-infected goats. Clin Vaccine Immunol. 2009;16:1003-11 pubmed publisher
Madoiwa S, Yamauchi T, Kobayashi E, Hakamata Y, Dokai M, Makino N, et al. Induction of factor VIII-specific unresponsiveness by intrathymic factor VIII injection in murine hemophilia A. J Thromb Haemost. 2009;7:811-24 pubmed publisher
Li Q, Shen H. Neonatal bacillus Calmette-Guérin vaccination inhibits de novo allergic inflammatory response in mice via alteration of CD4+CD25+ T-regulatory cells. Acta Pharmacol Sin. 2009;30:125-33 pubmed publisher
Samon J, Champhekar A, Minter L, Telfer J, Miele L, Fauq A, et al. Notch1 and TGFbeta1 cooperatively regulate Foxp3 expression and the maintenance of peripheral regulatory T cells. Blood. 2008;112:1813-21 pubmed publisher
Imamichi H, Sereti I, Lane H. IL-15 acts as a potent inducer of CD4(+)CD25(hi) cells expressing FOXP3. Eur J Immunol. 2008;38:1621-30 pubmed publisher
Coleman C, Muller Trutwin M, Apetrei C, Pandrea I. T regulatory cells: aid or hindrance in the clearance of disease?. J Cell Mol Med. 2007;11:1291-325 pubmed publisher
Lin S, Chen K, Lin C, Kuo C, Ling Q, Chan C. The quantitative analysis of peripheral blood FOXP3-expressing T cells in systemic lupus erythematosus and rheumatoid arthritis patients. Eur J Clin Invest. 2007;37:987-96 pubmed
Savai R, Schermuly R, Pullamsetti S, Schneider M, Greschus S, Ghofrani H, et al. A combination hybrid-based vaccination/adoptive cellular therapy to prevent tumor growth by involvement of T cells. Cancer Res. 2007;67:5443-53 pubmed
Chen C, Rowell E, Thomas R, Hancock W, Wells A. Transcriptional regulation by Foxp3 is associated with direct promoter occupancy and modulation of histone acetylation. J Biol Chem. 2006;281:36828-34 pubmed
Sakaguchi S. The origin of FOXP3-expressing CD4+ regulatory T cells: thymus or periphery. J Clin Invest. 2003;112:1310-2 pubmed
product information
Product Type :
Antibody
Product Name :
FOXP3 Monoclonal Antibody (eBio7979 (221D/D3)), eBioscience™
Catalog # :
14-7979-80
Quantity :
25 µg
Price :
US 169
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Human, Mouse
Applications :
Western Blot: 1-2 µg/mL
Species :
Human, Mouse
Clone :
eBio7979 (221D/D3)
Isotype :
IgG1
Storage :
4° C
Description :
FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.
Format :
Liquid
Applications w/Dilutions :
Western Blot: 1-2 µg/mL
Aliases :
AIID; DIETER; forkhead box P3; forkhead box protein P3; Forkhead box protein P3 41 kDa form; Forkhead box protein P3, C-terminally processed; forkhead/winged helix transcription factor 3; Foxp3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; IPEX; JM2; MGC141961; MGC141963; PIDX; regulatory protein Foxp3; RGD1562112; RP23-54C14.1; scurfin; scurfy; sf; XPID
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA