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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
TNF alpha Monoclonal Antibody (1F3F3D4), eBioscience™
catalog :
14-7325-85
quantity :
500 µg
price :
US 360
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
1F3F3D4
reactivity :
mouse
application :
ELISA, immunohistochemistry - frozen section
more info or order :
Invitrogen product webpage
citations: 26
Published Application/Species/Sample/DilutionReference
  • ELISA; mouse; 1.5 ug/ml; fig 1b
Majer O, Liu B, Kreuk L, Krogan N, Barton G. UNC93B1 recruits syntenin-1 to dampen TLR7 signalling and prevent autoimmunity. Nature. 2019;575:366-370 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 3j
Wang X, Chen H, Tian R, Zhang Y, Drutskaya M, Wang C, et al. Macrophages induce AKT/β-catenin-dependent Lgr5+ stem cell activation and hair follicle regeneration through TNF. Nat Commun. 2017;8:14091 pubmed publisher
  • ELISA; mouse; 1:1000; loading ...; fig s5a
Ulland T, Jain N, Hornick E, Elliott E, Clay G, Sadler J, et al. Nlrp12 mutation causes C57BL/6J strain-specific defect in neutrophil recruitment. Nat Commun. 2016;7:13180 pubmed publisher
  • ELISA; mouse; 1:500; fig 5e
Haan N, Zhu B, Wang J, Wei X, Song B. Crosstalk between macrophages and astrocytes affects proliferation, reactive phenotype and inflammatory response, suggesting a role during reactive gliosis following spinal cord injury. J Neuroinflammation. 2015;12:109 pubmed publisher
  • ELISA; mouse; fig 5
Pannu J, Belle J, Forster M, Duerr C, Shen S, Kane L, et al. Ubiquitin specific protease 21 is dispensable for normal development, hematopoiesis and lymphocyte differentiation. PLoS ONE. 2015;10:e0117304 pubmed publisher
  • ELISA; mouse; fig 4
Kim K, Kim N, Kim S, Kim I, Kim K, Lee G. Cyclo(Phe-Pro) produced by the human pathogen Vibrio vulnificus inhibits host innate immune responses through the NF-κB pathway. Infect Immun. 2015;83:1150-61 pubmed publisher
  • ELISA; mouse; fig 7b
Nijnik A, Madera L, Ma S, Waldbrook M, Elliott M, Easton D, et al. Synthetic cationic peptide IDR-1002 provides protection against bacterial infections through chemokine induction and enhanced leukocyte recruitment. J Immunol. 2010;184:2539-50 pubmed publisher
Yan J, Zhang Y, Du S, Hou X, Li W, Zeng C, et al. Nanomaterials-Mediated Co-Stimulation of Toll-Like Receptors and CD40 for Antitumor Immunity. Adv Mater. 2022;34:e2207486 pubmed publisher
Dai D, Yuan J, Wang Y, Xu J, Mao C, Xiao Y. Peli1 controls the survival of dopaminergic neurons through modulating microglia-mediated neuroinflammation. Sci Rep. 2019;9:8034 pubmed publisher
Leroux L, Nasr M, Valanparambil R, Tam M, Rosa B, Siciliani E, et al. Analysis of the Trichuris suis excretory/secretory proteins as a function of life cycle stage and their immunomodulatory properties. Sci Rep. 2018;8:15921 pubmed publisher
Ates L, Sayes F, Frigui W, Ummels R, Damen M, Bottai D, et al. RD5-mediated lack of PE_PGRS and PPE-MPTR export in BCG vaccine strains results in strong reduction of antigenic repertoire but little impact on protection. PLoS Pathog. 2018;14:e1007139 pubmed publisher
Ono K, Nimura S, Hideshima Y, Nabeshima K, Nakashima M. Orally administered sodium 4-phenylbutyrate suppresses the development of dextran sulfate sodium-induced colitis in mice. Exp Ther Med. 2017;14:5485-5490 pubmed publisher
Sayes F, Pawlik A, Frigui W, Gröschel M, Crommelynck S, Fayolle C, et al. CD4+ T Cells Recognizing PE/PPE Antigens Directly or via Cross Reactivity Are Protective against Pulmonary Mycobacterium tuberculosis Infection. PLoS Pathog. 2016;12:e1005770 pubmed publisher
Shao L, Zhou H, Zhang H, Qin L, Hwa J, Yun Z, et al. SENP1-mediated NEMO deSUMOylation in adipocytes limits inflammatory responses and type-1 diabetes progression. Nat Commun. 2015;6:8917 pubmed publisher
Komatsu N, Okamoto K, Sawa S, Nakashima T, Oh Hora M, Kodama T, et al. Pathogenic conversion of Foxp3+ T cells into TH17 cells in autoimmune arthritis. Nat Med. 2014;20:62-8 pubmed publisher
Escobar A, Candia E, Reyes Cerpa S, Villegas Valdes B, Neira T, Lopez M, et al. Neisseria gonorrhoeae induces a tolerogenic phenotype in macrophages to modulate host immunity. Mediators Inflamm. 2013;2013:127017 pubmed publisher
Nijnik A, Clare S, Hale C, Chen J, Raisen C, Mottram L, et al. The role of sphingosine-1-phosphate transporter Spns2 in immune system function. J Immunol. 2012;189:102-11 pubmed publisher
Levy D, Kuo A, Chang Y, Schaefer U, Kitson C, Cheung P, et al. Lysine methylation of the NF-κB subunit RelA by SETD6 couples activity of the histone methyltransferase GLP at chromatin to tonic repression of NF-κB signaling. Nat Immunol. 2011;12:29-36 pubmed publisher
Nijnik A, Pistolic J, Wyatt A, Tam S, Hancock R. Human cathelicidin peptide LL-37 modulates the effects of IFN-gamma on APCs. J Immunol. 2009;183:5788-98 pubmed publisher
Aleyas A, George J, Han Y, Rahman M, Kim S, Han S, et al. Functional modulation of dendritic cells and macrophages by Japanese encephalitis virus through MyD88 adaptor molecule-dependent and -independent pathways. J Immunol. 2009;183:2462-74 pubmed publisher
Amsen D, de Visser K, Town T. Approaches to determine expression of inflammatory cytokines. Methods Mol Biol. 2009;511:107-42 pubmed publisher
Khramova D, Popov S, Golovchenko V, Vityazev F, Paderin N, Ovodov Y. Abrogation of the oral tolerance to ovalbumin in mice by citrus pectin. Nutrition. 2009;25:226-32 pubmed publisher
Goforth R, Salem A, Zhu X, Miles S, Zhang X, Lee J, et al. Immune stimulatory antigen loaded particles combined with depletion of regulatory T-cells induce potent tumor specific immunity in a mouse model of melanoma. Cancer Immunol Immunother. 2009;58:517-30 pubmed publisher
Castilow E, Olson M, Meyerholz D, Varga S. Differential role of gamma interferon in inhibiting pulmonary eosinophilia and exacerbating systemic disease in fusion protein-immunized mice undergoing challenge infection with respiratory syncytial virus. J Virol. 2008;82:2196-207 pubmed
Popov S, Popova G, Paderin N, Koval O, Ovodova R, Ovodov Y. Preventative antiinflammatory effect of potamogetonan, a pectin from the common pondweed Potamogeton natans L. Phytother Res. 2007;21:609-14 pubmed
Lucas R, Heirwegh K, Neirynck A, Remels L, Van Heuverswyn H, De Baetselier P. Generation and characterization of a neutralizing rat anti-rmTNF-alpha monoclonal antibody. Immunology. 1990;71:218-23 pubmed
product information
Product Type :
Antibody
Product Name :
TNF alpha Monoclonal Antibody (1F3F3D4), eBioscience™
Catalog # :
14-7325-85
Quantity :
500 µg
Price :
US 360
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
ELISA: 1-4 µg/mL
Species :
Mouse
Clone :
1F3F3D4
Isotype :
IgM, kappa
Storage :
4° C
Description :
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
Format :
Liquid
Applications w/Dilutions :
ELISA: 1-4 µg/mL
Aliases :
APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; Tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA