IFN gamma Monoclonal Antibody (MD-1), eBioscience™ | Invitrogen 14-7317-85 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

IFN gamma Monoclonal Antibody (MD-1), eBioscience™

catalog :

14-7317-85

quantity :

500 µg

price :

US 302

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MD-1

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 24

Published Application/Species/Sample/Dilution	Reference
blocking or activating experiments; human; 5 ug/ml; fig 7	van Wilgenburg B, Scherwitzl I, Hutchinson E, Leng T, Kurioka A, Kulicke C, et al. MAIT cells are activated during human viral infections. Nat Commun. 2016;7:11653 pubmed publisher
	Bounab Y, Eyer K, Dixneuf S, Rybczynska M, Chauvel C, Mistretta M, et al. Dynamic single-cell phenotyping of immune cells using the microfluidic platform DropMap. Nat Protoc. 2020;15:2920-2955 pubmed publisher
	Lee J, Hall J, Kroehling L, Wu L, Najar T, Nguyen H, et al. Serum Amyloid A Proteins Induce Pathogenic Th17 Cells and Promote Inflammatory Disease. Cell. 2020;180:79-91.e16 pubmed publisher
	Voos P, Fuck S, Weipert F, Babel L, Tandl D, Meckel T, et al. Ionizing Radiation Induces Morphological Changes and Immunological Modulation of Jurkat Cells. Front Immunol. 2018;9:922 pubmed publisher
	Zhao M, Tan Y, Peng Q, Huang C, Guo Y, Liang G, et al. IL-6/STAT3 pathway induced deficiency of RFX1 contributes to Th17-dependent autoimmune diseases via epigenetic regulation. Nat Commun. 2018;9:583 pubmed publisher
	Tuohy V, Jaini R, Johnson J, Loya M, Wilk D, Downs Kelly E, et al. Targeted Vaccination against Human ?-Lactalbumin for Immunotherapy and Primary Immunoprevention of Triple Negative Breast Cancer. Cancers (Basel). 2016;8: pubmed publisher
	Shen H, Wang Y, Chen C, Frencher J, Huang D, Yang E, et al. Th17-related cytokines contribute to recall-like expansion/effector function of HMBPP-specific VÎ³2VÎ´2 T cells after Mycobacterium tuberculosis infection or vaccination. Eur J Immunol. 2015;45:442-51 pubmed publisher
	Price J, Haddon D, Kemmer D, Delepine G, Mandelbaum G, Jarrell J, et al. Protein microarray analysis reveals BAFF-binding autoantibodies in systemic lupus erythematosus. J Clin Invest. 2013;123:5135-45 pubmed publisher
	Blom L, Poulsen L. In vitro Th1 and Th2 cell polarization is severely influenced by the initial ratio of naïve and memory CD4+ T cells. J Immunol Methods. 2013;397:55-60 pubmed publisher
	Liu B, Zhang N, Liu Z, Fu Y, Feng S, Wang S, et al. RP105 involved in activation of mouse macrophages via TLR2 and TLR4 signaling. Mol Cell Biochem. 2013;378:183-93 pubmed publisher
	Blom L, Poulsen L. IL-1 family members IL-18 and IL-33 upregulate the inflammatory potential of differentiated human Th1 and Th2 cultures. J Immunol. 2012;189:4331-7 pubmed publisher
	Arikata M, Itoh Y, Okamatsu M, Maeda T, Shiina T, Tanaka K, et al. Memory immune responses against pandemic (H1N1) 2009 influenza virus induced by a whole particle vaccine in cynomolgus monkeys carrying Mafa-A1*052:02. PLoS ONE. 2012;7:e37220 pubmed publisher
	Sasaki S, Nagai Y, Yanagibashi T, Watanabe Y, Ikutani M, Kariyone A, et al. Serum soluble MD-1 levels increase with disease progression in autoimmune prone MRL(lpr/lpr) mice. Mol Immunol. 2012;49:611-20 pubmed publisher
	Blom L, Poulsen B, Jensen B, Hansen A, Poulsen L. IL-33 induces IL-9 production in human CD4+ T cells and basophils. PLoS ONE. 2011;6:e21695 pubmed publisher
	Yao S, Huang D, Chen C, Halliday L, Zeng G, Wang R, et al. Differentiation, distribution and gammadelta T cell-driven regulation of IL-22-producing T cells in tuberculosis. PLoS Pathog. 2010;6:e1000789 pubmed publisher
	Blumenthal A, Kobayashi T, Pierini L, Banaei N, Ernst J, Miyake K, et al. RP105 facilitates macrophage activation by Mycobacterium tuberculosis lipoproteins. Cell Host Microbe. 2009;5:35-46 pubmed publisher
	Gong G, Shao L, Wang Y, Chen C, Huang D, Yao S, et al. Phosphoantigen-activated V gamma 2V delta 2 T cells antagonize IL-2-induced CD4+CD25+Foxp3+ T regulatory cells in mycobacterial infection. Blood. 2009;113:837-45 pubmed publisher
	Jurado J, Alvarez I, Pasquinelli V, Martinez G, Quiroga M, Abbate E, et al. Programmed death (PD)-1:PD-ligand 1/PD-ligand 2 pathway inhibits T cell effector functions during human tuberculosis. J Immunol. 2008;181:116-25 pubmed
	Wolint P, Betts M, Koup R, Oxenius A. Immediate cytotoxicity but not degranulation distinguishes effector and memory subsets of CD8+ T cells. J Exp Med. 2004;199:925-36 pubmed
	van der Meide P, Groenestein R, de Labie M, Heeney J, Pala P, Slaoui M. Enumeration of lymphokine-secreting cells as a quantitative measure for cellular immune responses in rhesus macaques. J Med Primatol. 1995;24:271-81 pubmed
	van Besouw N, van der Meide P, Bakker N. The mitogen-induced generation of interferon-gamma producing cells in cultures of rhesus monkey peripheral blood mononuclear cells is age-dependent. J Med Primatol. 1994;23:42-8 pubmed
	Yazdanbakhsh M, Sartono E, Kruize Y, Kurniawan A, van der Pouw Kraan T, van der Meide P, et al. Elevated levels of T cell activation antigen CD27 and increased interleukin-4 production in human lymphatic filariasis. Eur J Immunol. 1993;23:3312-7 pubmed
	Wassenaar A, Reinhardus C, Thepen T, Abraham Inpijn L, Kievits F. Cloning, characterization, and antigen specificity of T-lymphocyte subsets extracted from gingival tissue of chronic adult periodontitis patients. Infect Immun. 1995;63:2147-53 pubmed
	van der Meide P, Dubbeld M, Schellekens H. Monoclonal antibodies to human immune interferon and their use in a sensitive solid-phase ELISA. J Immunol Methods. 1985;79:293-305 pubmed

product information

Product Type :

Antibody

Product Name :

IFN gamma Monoclonal Antibody (MD-1), eBioscience™

Catalog # :

14-7317-85

Quantity :

500 µg

Price :

US 302

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human, Non-human primate

Applications :

ELISA: 1-4 µg/mL, Flow Cytometry: Assay-Dependent, Immunohistochemistry (Frozen): Assay-Dependent, Western Blot: Assay-Dependent

Species :

Human, Non-human primate

Clone :

MD-1

Isotype :

IgG1, kappa

Storage :

4° C

Description :

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

Format :

Liquid

Applications w/Dilutions :

ELISA: 1-4 µg/mL, Flow Cytometry: Assay-Dependent, Immunohistochemistry (Frozen): Assay-Dependent, Western Blot: Assay-Dependent

Aliases :

BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; Ifg; IFI; IFN gamma; IFN ?; IFNG; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFN?; Immune interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon ?; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferon?; M-IFN-g; R-IFN-g

more info or order :

Invitrogen product webpage