CCL2 (MCP-1) Monoclonal Antibody (5D3-F7), eBioscience™ | Invitrogen 14-7099-81 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CCL2 (MCP-1) Monoclonal Antibody (5D3-F7), eBioscience™

catalog :

14-7099-81

quantity :

50µg

price :

US 96

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5D3-F7

reactivity :

human

application :

ELISA, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 21

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 3c	Kim J, Jeong J, Jung J, Jeon H, Lee S, Lim J, et al. Immunological characteristics and possible pathogenic role of urinary CD11c+ macrophages in lupus nephritis. Rheumatology (Oxford). 2020;: pubmed publisher
ELISA; human; fig 1b	Nijnik A, Madera L, Ma S, Waldbrook M, Elliott M, Easton D, et al. Synthetic cationic peptide IDR-1002 provides protection against bacterial infections through chemokine induction and enhanced leukocyte recruitment. J Immunol. 2010;184:2539-50 pubmed publisher
	Ruiz Silva M, van der Ende Metselaar H, Mulder H, Smit J, Rodenhuis Zybert I. Mechanism and role of MCP-1 upregulation upon chikungunya virus infection in human peripheral blood mononuclear cells. Sci Rep. 2016;6:32288 pubmed publisher
	Harmon D, Srikakulapu P, Kaplan J, Oldham S, McSkimming C, Garmey J, et al. Protective Role for B-1b B Cells and IgM in Obesity-Associated Inflammation, Glucose Intolerance, and Insulin Resistance. Arterioscler Thromb Vasc Biol. 2016;36:682-91 pubmed publisher
	Park A, Huang G, Jankowska Gan E, Massoudi D, Kernien J, VIGNALI D, et al. Mucosal Administration of Collagen V Ameliorates the Atherosclerotic Plaque Burden by Inducing Interleukin 35-dependent Tolerance. J Biol Chem. 2016;291:3359-70 pubmed publisher
	Valera E, Shia W, Bailey R. Development and validation of an immunosensor for monocyte chemotactic protein 1 using a silicon photonic microring resonator biosensing platform. Clin Biochem. 2016;49:121-6 pubmed publisher
	Moyon S, Dubessy A, Aigrot M, Trotter M, Huang J, Dauphinot L, et al. Demyelination causes adult CNS progenitors to revert to an immature state and express immune cues that support their migration. J Neurosci. 2015;35:4-20 pubmed publisher
	Bekkering S, Quintin J, Joosten L, Van der Meer J, Netea M, Riksen N. Oxidized low-density lipoprotein induces long-term proinflammatory cytokine production and foam cell formation via epigenetic reprogramming of monocytes. Arterioscler Thromb Vasc Biol. 2014;34:1731-8 pubmed publisher
	Price J, Haddon D, Kemmer D, Delepine G, Mandelbaum G, Jarrell J, et al. Protein microarray analysis reveals BAFF-binding autoantibodies in systemic lupus erythematosus. J Clin Invest. 2013;123:5135-45 pubmed publisher
	Spaan M, Groothuismink Z, Koning L, Roomer R, Janssen H, de Knegt R, et al. Erythropoietin administration suppresses human monocyte function in vitro and during therapy-induced anemia in HCV patients. Antiviral Res. 2013;98:469-75 pubmed publisher
	Arana L, Ordoñez M, Ouro A, Rivera I, Gangoiti P, Trueba M, et al. Ceramide 1-phosphate induces macrophage chemoattractant protein-1 release: involvement in ceramide 1-phosphate-stimulated cell migration. Am J Physiol Endocrinol Metab. 2013;304:E1213-26 pubmed publisher
	Gellatly S, Needham B, Madera L, Trent M, Hancock R. The Pseudomonas aeruginosa PhoP-PhoQ two-component regulatory system is induced upon interaction with epithelial cells and controls cytotoxicity and inflammation. Infect Immun. 2012;80:3122-31 pubmed publisher
	Nijnik A, Pistolic J, Cho P, Filewod N, Falsafi R, Ramin A, et al. The role of the Src family kinase Lyn in the immunomodulatory activities of cathelicidin peptide LL-37 on monocytic cells. J Leukoc Biol. 2012;91:599-607 pubmed publisher
	Peng C, Liu B, de Knegt R, Janssen H, Boonstra A. The response to TLR ligation of human CD16?CD14? monocytes is weakly modulated as a consequence of persistent infection with the hepatitis C virus. Mol Immunol. 2011;48:1505-11 pubmed publisher
	Cimmino G, Ibanez B, Giannarelli C, Prat Gonzalez S, Hutter R, Garcia M, et al. Carvedilol administration in acute myocardial infarction results in stronger inhibition of early markers of left ventricular remodeling than metoprolol. Int J Cardiol. 2011;153:256-61 pubmed publisher
	Hu H, Sun L, Guo C, Liu Q, Zhou Z, Peng L, et al. Tumor cell-microenvironment interaction models coupled with clinical validation reveal CCL2 and SNCG as two predictors of colorectal cancer hepatic metastasis. Clin Cancer Res. 2009;15:5485-93 pubmed publisher
	Robey R, Shukla S, Steadman K, Obrzut T, Finley E, Ambudkar S, et al. Inhibition of ABCG2-mediated transport by protein kinase inhibitors with a bisindolylmaleimide or indolocarbazole structure. Mol Cancer Ther. 2007;6:1877-85 pubmed
	Karrer S, Bosserhoff A, Weiderer P, Distler O, Landthaler M, Szeimies R, et al. The -2518 promotor polymorphism in the MCP-1 gene is associated with systemic sclerosis. J Invest Dermatol. 2005;124:92-8 pubmed
	Zhou S, Zong Y, Ney P, Nair G, Stewart C, Sorrentino B. Increased expression of the Abcg2 transporter during erythroid maturation plays a role in decreasing cellular protoporphyrin IX levels. Blood. 2005;105:2571-6 pubmed
	Piemonti L, Leone B, Nano R, Saccani A, Monti P, Maffi P, et al. Human pancreatic islets produce and secrete MCP-1/CCL2: relevance in human islet transplantation. Diabetes. 2002;51:55-65 pubmed
	Peri G, Milanese C, Matteucci C, Ruco L, Zhou D, Sozzani S, et al. A new monoclonal antibody (5D3-F7) which recognizes human monocyte-chemotactic protein-1 but not related chemokines. Development of a sandwich ELISA and in situ detection of producing cells. J Immunol Methods. 1994;174:249-57 pubmed

product information

Product Type :

Antibody

Product Name :

CCL2 (MCP-1) Monoclonal Antibody (5D3-F7), eBioscience™

Catalog # :

14-7099-81

Quantity :

50µg

Price :

US 96

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human

Applications :

ELISA: 1-4 µg/mL, Flow Cytometry: Assay-Dependent, Immunohistochemistry (Paraffin): Assay-Dependent, Immunoprecipitation: Assay-Dependent

Species :

Human

Clone :

5D3-F7

Isotype :

IgG1, kappa

Storage :

4° C

Description :

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

Format :

Liquid

Applications w/Dilutions :

ELISA: 1-4 µg/mL, Flow Cytometry: Assay-Dependent, Immunohistochemistry (Paraffin): Assay-Dependent, Immunoprecipitation: Assay-Dependent

Aliases :

Acidic seminal fluid protein; AI323594; BOS_18463; C-C motif chemokine 2; C-C motif chemokine ligand 2; Ccl2; chemokine (C-C motif) ligand 2; GDCF-2; HC11; H-MCP-1; HSMCR30; immediate-early serum-responsive JE protein; Immediate-early serum-responsive protein JE; Je; MCAF; Mcp1; MCP-1; MCP1A; MCP-1A; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte chemotactic protein 1A; Monocyte secretory protein JE; Platelet-derived growth factor-inducible protein JE; RP23-350G1.3; Scya2; Sigje; small inducible cytokine A2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible gene JE; Small-inducible cytokine A2; SMC-CF

more info or order :

Invitrogen product webpage