Bcl-2 Monoclonal Antibody (10C4), eBioscience | Invitrogen 14-6992-82 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Bcl-2 Monoclonal Antibody (10C4), eBioscience

catalog :

14-6992-82

quantity :

100 µg

price :

US 219

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

10C4

reactivity :

mouse, rat

application :

western blot, immunoprecipitation, flow cytometry

more info or order :

Invitrogen product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 6d	Visekruna A, Hartmann S, Sillke Y, Glauben R, Fischer F, Raifer H, et al. Intestinal development and homeostasis require activation and apoptosis of diet-reactive T cells. J Clin Invest. 2019;129:1972-1983 pubmed publisher
flow cytometry; mouse; 1:200; loading ...; fig 5e	Zhu L, Xie X, Zhang L, Wang H, Jie Z, Zhou X, et al. TBK-binding protein 1 regulates IL-15-induced autophagy and NKT cell survival. Nat Commun. 2018;9:2812 pubmed publisher
	Rudak P, Choi J, Parkins K, Summers K, Jackson D, Foster P, et al. Chronic stress physically spares but functionally impairs innate-like invariant T cells. Cell Rep. 2021;35:108979 pubmed publisher
	Hosking M, Flynn C, Botten J, Whitton J. CD8+ memory T cells appear exhausted within hours of acute virus infection. J Immunol. 2013;191:4211-22 pubmed publisher
	Bezman N, Kim C, Sun J, Min oo G, Hendricks D, Kamimura Y, et al. Molecular definition of the identity and activation of natural killer cells. Nat Immunol. 2012;13:1000-9 pubmed publisher
	McFarland H, Puig M, Grajkowska L, Tsuji K, Lee J, Mason K, et al. Regulatory T cells in ? irradiation-induced immune suppression. PLoS ONE. 2012;7:e39092 pubmed publisher
	Notake T, Horisawa S, Sanjo H, Miyagawa S, Hida S, Taki S. Differential requirements for IRF-2 in generation of CD1d-independent T cells bearing NK cell receptors. J Immunol. 2012;188:4838-45 pubmed publisher
	Wang X, Szymczak Workman A, Gravano D, Workman C, Green D, Vignali D. Preferential control of induced regulatory T cell homeostasis via a Bim/Bcl-2 axis. Cell Death Dis. 2012;3:e270 pubmed publisher
	Lawlor K, Smith S, van Nieuwenhuijze A, Huang D, Wicks I. Evaluation of the Bcl-2 family antagonist ABT-737 in collagen-induced arthritis. J Leukoc Biol. 2011;90:819-29 pubmed publisher
	Yewdall A, DRUTMAN S, Jinwala F, Bahjat K, Bhardwaj N. CD8+ T cell priming by dendritic cell vaccines requires antigen transfer to endogenous antigen presenting cells. PLoS ONE. 2010;5:e11144 pubmed publisher
	Sgouroudis E, Albanese A, Piccirillo C. Impact of protective IL-2 allelic variants on CD4+ Foxp3+ regulatory T cell function in situ and resistance to autoimmune diabetes in NOD mice. J Immunol. 2008;181:6283-92 pubmed
	GRATIOT DEANS J, Ding L, Turka L, Nunez G. bcl-2 proto-oncogene expression during human T cell development. Evidence for biphasic regulation. J Immunol. 1993;151:83-91 pubmed
	Hockenbery D, Nunez G, Milliman C, Schreiber R, Korsmeyer S. Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature. 1990;348:334-6 pubmed

product information

Product Type :

Antibody

Product Name :

Bcl-2 Monoclonal Antibody (10C4), eBioscience

Catalog # :

14-6992-82

Quantity :

100 µg

Price :

US 219

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Mouse, Rat

Applications :

Flow Cytometry: Assay-Dependent, Immunoprecipitation: Assay-Dependent, Western Blot: 0.5-10 µg/mL

Species :

Mouse, Rat

Clone :

10C4

Isotype :

IgG1, kappa

Storage :

4° C

Description :

BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-Dependent, Immunoprecipitation: Assay-Dependent, Western Blot: 0.5-10 µg/mL

Aliases :

apoptosis regulator Bcl-2; AW986256; B cell leukemia/lymphoma 2; B cell lymphoma 2 associated oncogene; B-cell CLL/lymphoma 2; B-cell leukemia/lymphoma 2; Bcl 2; BCL2; bcl-2; BCL2 apoptosis regulator; Bcl-2 protein; BCL2, apoptosis regulator; Bcl2-like protein; C430015F12Rik; D630044D05Rik; D830018M01Rik; OTTHUMP00000163680; PPP1R50; protein phosphatase 1, regulatory subunit 50

more info or order :

Invitrogen product webpage