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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
ROR gamma (t) Monoclonal Antibody (B2D), eBioscience
catalog :
14-6981-82
quantity :
100 µg
price :
US 514
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
B2D
reactivity :
human, mouse
application :
western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 65
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 2c
Muro R, Nitta T, Nakano K, Okamura T, Takayanagi H, Suzuki H. γδTCR recruits the Syk/PI3K axis to drive proinflammatory differentiation program. J Clin Invest. 2018;128:415-426 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3d
Meinicke H, Bremser A, Brack M, Akeus P, Pearson C, Bullers S, et al. Tumour-associated changes in intestinal epithelial cells cause local accumulation of KLRG1+ GATA3+ regulatory T cells in mice. Immunology. 2017;152:74-88 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2b
Lino C, Barros Martins J, Oberdörfer L, Walzer T, Prinz I. Eomes expression reports the progressive differentiation of IFN-?-producing Th1-like ?? T cells. Eur J Immunol. 2017;47:970-981 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6a
Shirakawa K, Yan X, Shinmura K, Endo J, Kataoka M, Katsumata Y, et al. Obesity accelerates T cell senescence in murine visceral adipose tissue. J Clin Invest. 2016;126:4626-4639 pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig 1d
Georgiev H, Ravens I, Benarafa C, Forster R, Bernhardt G. Distinct gene expression patterns correlate with developmental and functional traits of iNKT subsets. Nat Commun. 2016;7:13116 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6e
Lexmond W, Goettel J, Lyons J, Jacobse J, Deken M, Lawrence M, et al. FOXP3+ Tregs require WASP to restrain Th2-mediated food allergy. J Clin Invest. 2016;126:4030-4044 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a, s5c, s18a
Gomez de Agüero M, Ganal Vonarburg S, Fuhrer T, Rupp S, Uchimura Y, Li H, et al. The maternal microbiota drives early postnatal innate immune development. Science. 2016;351:1296-302 pubmed publisher
  • immunohistochemistry; mouse; fig 1
Caballero Franco C, Guma M, Choo M, Sano Y, Enzler T, Karin M, et al. Epithelial Control of Gut-Associated Lymphoid Tissue Formation through p38?-Dependent Restraint of NF-?B Signaling. J Immunol. 2016;196:2368-76 pubmed publisher
  • flow cytometry; mouse; fig s4
Kuipers H, Rieck M, Gurevich I, Nagy N, Butte M, Negrin R, et al. Hyaluronan synthesis is necessary for autoreactive T-cell trafficking, activation, and Th1 polarization. Proc Natl Acad Sci U S A. 2016;113:1339-44 pubmed publisher
  • western blot; mouse; 1:250; fig 4
Castiglioni A, Corna G, Rigamonti E, Basso V, Vezzoli M, Monno A, et al. FOXP3+ T Cells Recruited to Sites of Sterile Skeletal Muscle Injury Regulate the Fate of Satellite Cells and Guide Effective Tissue Regeneration. PLoS ONE. 2015;10:e0128094 pubmed publisher
  • flow cytometry; mouse; fig 3
Hernández P, Mahlakõiv T, Yang I, Schwierzeck V, Nguyen N, Guendel F, et al. Interferon-λ and interleukin 22 act synergistically for the induction of interferon-stimulated genes and control of rotavirus infection. Nat Immunol. 2015;16:698-707 pubmed publisher
  • immunohistochemistry - paraffin section; human; fig s8
Chen H, Sun J, Huang Z, Hou H, Arcilla M, Rakhilin N, et al. Comprehensive models of human primary and metastatic colorectal tumors in immunodeficient and immunocompetent mice by chemokine targeting. Nat Biotechnol. 2015;33:656-60 pubmed publisher
  • immunohistochemistry - frozen section; human
Noort A, van Zoest K, van Baarsen L, Maracle C, Helder B, Papazian N, et al. Tertiary Lymphoid Structures in Rheumatoid Arthritis: NF-κB-Inducing Kinase-Positive Endothelial Cells as Central Players. Am J Pathol. 2015;185:1935-43 pubmed publisher
  • western blot; mouse; 1:2000; fig 5,6
Hsiao H, Hsu T, Liu W, Hsieh W, Chou T, Wu Y, et al. Deltex1 antagonizes HIF-1α and sustains the stability of regulatory T cells in vivo. Nat Commun. 2015;6:6353 pubmed publisher
  • western blot; mouse; 1:200
Dong B, Li D, Li R, Chen S, Liu W, Liu W, et al. A chitin-like component on sclerotic cells of Fonsecaea pedrosoi inhibits Dectin-1-mediated murine Th17 development by masking β-glucans. PLoS ONE. 2014;9:e114113 pubmed publisher
  • flow cytometry; mouse; fig 3c
  • western blot; mouse; fig 3f
Rutz S, Kayagaki N, Phung Q, Eidenschenk C, Noubade R, Wang X, et al. Deubiquitinase DUBA is a post-translational brake on interleukin-17 production in T cells. Nature. 2015;518:417-21 pubmed publisher
  • flow cytometry; mouse
Vogelzang A, Perdomo C, Zedler U, Kuhlmann S, Hurwitz R, Gengenbacher M, et al. Central memory CD4+ T cells are responsible for the recombinant Bacillus Calmette-Guérin ?ureC::hly vaccine's superior protection against tuberculosis. J Infect Dis. 2014;210:1928-37 pubmed publisher
  • flow cytometry; mouse
Samuelson E, Laird R, Papillion A, Tatum A, Princiotta M, Hayes S. Reduced B lymphoid kinase (Blk) expression enhances proinflammatory cytokine production and induces nephrosis in C57BL/6-lpr/lpr mice. PLoS ONE. 2014;9:e92054 pubmed publisher
  • flow cytometry; mouse
McGuire D, Rowse A, Li H, Peng B, Sestero C, Cashman K, et al. CD5 enhances Th17-cell differentiation by regulating IFN-? response and ROR?t localization. Eur J Immunol. 2014;44:1137-42 pubmed publisher
Hu G, Zhou X. Gallic Acid Ameliorates Atopic Dermatitis-Like Skin Inflammation Through Immune Regulation in a Mouse Model. Clin Cosmet Investig Dermatol. 2021;14:1675-1683 pubmed publisher
Kim J, Suh Y, Chang K. Interleukin-17 induced by cumulative mild stress promoted depression-like behaviors in young adult mice. Mol Brain. 2021;14:11 pubmed publisher
Hayashi K, Nikolos F, Lee Y, Jain A, Tsouko E, Gao H, et al. Tipping the immunostimulatory and inhibitory DAMP balance to harness immunogenic cell death. Nat Commun. 2020;11:6299 pubmed publisher
Poholek C, Dulson S, Zajac A, Harrington L. IL-21 Controls ILC3 Cytokine Production and Promotes a Protective Phenotype in a Mouse Model of Colitis. Immunohorizons. 2019;3:194-202 pubmed publisher
Arnold I, Zhang X, Artola Borán M, Fallegger A, Sander P, Johansen P, et al. BATF3-dependent dendritic cells drive both effector and regulatory T-cell responses in bacterially infected tissues. PLoS Pathog. 2019;15:e1007866 pubmed publisher
Lavoz C, Matus Y, Orejudo M, Carpio J, Droguett A, Egido J, et al. Interleukin-17A blockade reduces albuminuria and kidney injury in an accelerated model of diabetic nephropathy. Kidney Int. 2019;95:1418-1432 pubmed publisher
Melo Gonzalez F, Kammoun H, Evren E, Dutton E, Papadopoulou M, Bradford B, et al. Antigen-presenting ILC3 regulate T cell-dependent IgA responses to colonic mucosal bacteria. J Exp Med. 2019;216:728-742 pubmed publisher
Mande P, Zirak B, Ko W, Taravati K, Bride K, Brodeur T, et al. Fas ligand promotes an inducible TLR-dependent model of cutaneous lupus-like inflammation. J Clin Invest. 2018;128:2966-2978 pubmed publisher
Gaya M, Barral P, Burbage M, Aggarwal S, Montaner B, Warren Navia A, et al. Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells. Cell. 2018;172:517-533.e20 pubmed publisher
Muschaweckh A, Petermann F, Korn T. IL-1? and IL-23 Promote Extrathymic Commitment of CD27+CD122- ?? T Cells to ??T17 Cells. J Immunol. 2017;199:2668-2679 pubmed publisher
Martin C, Spasova D, Frimpong Boateng K, Kim H, Lee M, Kim K, et al. Interleukin-7 Availability Is Maintained by a Hematopoietic Cytokine Sink Comprising Innate Lymphoid Cells and T Cells. Immunity. 2017;47:171-182.e4 pubmed publisher
Redhu N, Bakthavatchalu V, Conaway E, Shouval D, Tsou A, Goettel J, et al. Macrophage dysfunction initiates colitis during weaning of infant mice lacking the interleukin-10 receptor. elife. 2017;6: pubmed publisher
Levine A, Mendoza A, Hemmers S, Moltedo B, Niec R, Schizas M, et al. Stability and function of regulatory T cells expressing the transcription factor T-bet. Nature. 2017;546:421-425 pubmed publisher
Ding F, Liao R, Chen Y, Xie G, Zhang P, Shao P, et al. Upregulation of SOCS3 in lung CD4+ T cells in a mouse model of chronic PA lung infection and suppression of Th17‑mediated neutrophil recruitment in exogenous SOCS3 transfer in vitro. Mol Med Rep. 2017;16:778-786 pubmed publisher
Lopes N, Vachon H, Marie J, Irla M. Administration of RANKL boosts thymic regeneration upon bone marrow transplantation. EMBO Mol Med. 2017;9:835-851 pubmed publisher
Fu G, Xu Q, Qiu Y, Jin X, Xu T, Dong S, et al. Suppression of Th17 cell differentiation by misshapen/NIK-related kinase MINK1. J Exp Med. 2017;214:1453-1469 pubmed publisher
Chapman L, Ture S, Field D, Morrell C. miR-451 limits CD4+ T cell proliferative responses to infection in mice. Immunol Res. 2017;65:828-840 pubmed publisher
Hu Y, Lao L, Mao J, Jin W, Luo H, Charpentier T, et al. Armc5 deletion causes developmental defects and compromises T-cell immune responses. Nat Commun. 2017;8:13834 pubmed publisher
Chudnovskiy A, Mortha A, Kana V, Kennard A, Ramírez J, Rahman A, et al. Host-Protozoan Interactions Protect from Mucosal Infections through Activation of the Inflammasome. Cell. 2016;167:444-456.e14 pubmed publisher
Chou T, Chuang Y, Hsieh W, Chang P, Liu H, Mo S, et al. Tumour suppressor death-associated protein kinase targets cytoplasmic HIF-1? for Th17 suppression. Nat Commun. 2016;7:11904 pubmed publisher
Xing S, Li F, Zeng Z, Zhao Y, Yu S, Shan Q, et al. Tcf1 and Lef1 transcription factors establish CD8(+) T cell identity through intrinsic HDAC activity. Nat Immunol. 2016;17:695-703 pubmed publisher
Wang J, Peng L, Zhang R, Zheng Z, Chen C, Cheung K, et al. 5-Fluorouracil targets thymidylate synthase in the selective suppression of TH17 cell differentiation. Oncotarget. 2016;7:19312-26 pubmed publisher
Jones L, Alli R, Li B, Geiger T. Differential T Cell Cytokine Receptivity and Not Signal Quality Distinguishes IL-6 and IL-10 Signaling during Th17 Differentiation. J Immunol. 2016;196:2973-85 pubmed publisher
Corpuz T, Stolp J, Kim H, Pinget G, Gray D, Cho J, et al. Differential Responsiveness of Innate-like IL-17- and IFN-γ-Producing γδ T Cells to Homeostatic Cytokines. J Immunol. 2016;196:645-54 pubmed publisher
Lim H, Kang S, Ryu J, Schilling B, Fei M, Lee I, et al. SIRT1 deacetylates RORγt and enhances Th17 cell generation. J Exp Med. 2015;212:607-17 pubmed publisher
Reis B, Lee K, Fanok M, Mascaraque C, Amoury M, Cohn L, et al. Leptin receptor signaling in T cells is required for Th17 differentiation. J Immunol. 2015;194:5253-60 pubmed publisher
Bando J, Liang H, Locksley R. Identification and distribution of developing innate lymphoid cells in the fetal mouse intestine. Nat Immunol. 2015;16:153-60 pubmed publisher
Lynch L, Michelet X, Zhang S, Brennan P, Moseman A, Lester C, et al. Regulatory iNKT cells lack expression of the transcription factor PLZF and control the homeostasis of T(reg) cells and macrophages in adipose tissue. Nat Immunol. 2015;16:85-95 pubmed publisher
Linterman M, Denton A, Divekar D, Zvetkova I, Kane L, Ferreira C, et al. CD28 expression is required after T cell priming for helper T cell responses and protective immunity to infection. elife. 2014;3: pubmed publisher
Drummond R, Wallace C, Reid D, Way S, Kaplan D, Brown G. Cutting edge: Failure of antigen-specific CD4+ T cell recruitment to the kidney during systemic candidiasis. J Immunol. 2014;193:5381-5 pubmed publisher
Rodrígues Díez R, Aroeira L, Orejudo M, Bajo M, Heffernan J, Rodrigues Díez R, et al. IL-17A is a novel player in dialysis-induced peritoneal damage. Kidney Int. 2014;86:303-15 pubmed publisher
Li C, Mueller E, Bradley L. Islet antigen-specific Th17 cells can induce TNF-?-dependent autoimmune diabetes. J Immunol. 2014;192:1425-32 pubmed publisher
Kim H, Lee H, Chang Y, Pichavant M, Shore S, Fitzgerald K, et al. Interleukin-17-producing innate lymphoid cells and the NLRP3 inflammasome facilitate obesity-associated airway hyperreactivity. Nat Med. 2014;20:54-61 pubmed publisher
Chen Q, Yan L, Wang C, Wang W, Shi H, Su B, et al. Mesenchymal stem cells alleviate TNBS-induced colitis by modulating inflammatory and autoimmune responses. World J Gastroenterol. 2013;19:4702-17 pubmed publisher
Jianjun Yang -, Zhang R, Lu G, Shen Y, Peng L, Zhu C, et al. T cell–derived inducible nitric oxide synthase switches off Th17 cell differentiation. J Exp Med. 2013;210:1447-62 pubmed
Mielke L, Jones S, Raverdeau M, Higgs R, Stefanska A, Groom J, et al. Retinoic acid expression associates with enhanced IL-22 production by ?? T cells and innate lymphoid cells and attenuation of intestinal inflammation. J Exp Med. 2013;210:1117-24 pubmed publisher
Rodrígues Díez R, Rodrigues Díez R, Rayego Mateos S, Suarez Alvarez B, Lavoz C, Stark Aroeira L, et al. The C-terminal module IV of connective tissue growth factor is a novel immune modulator of the Th17 response. Lab Invest. 2013;93:812-24 pubmed publisher
Hermann Kleiter N, Meisel M, Fresser F, Thuille N, Muller M, Roth L, et al. Nuclear orphan receptor NR2F6 directly antagonizes NFAT and ROR?t binding to the Il17a promoter. J Autoimmun. 2012;39:428-40 pubmed publisher
Ichiyama K, Sekiya T, Inoue N, Tamiya T, Kashiwagi I, Kimura A, et al. Transcription factor Smad-independent T helper 17 cell induction by transforming-growth factor-? is mediated by suppression of eomesodermin. Immunity. 2011;34:741-54 pubmed publisher
Lazarevic V, Chen X, Shim J, Hwang E, Jang E, Bolm A, et al. T-bet represses T(H)17 differentiation by preventing Runx1-mediated activation of the gene encoding ROR?t. Nat Immunol. 2011;12:96-104 pubmed publisher
Pierau M, Engelmann S, Reinhold D, Lapp T, Schraven B, Bommhardt U. Protein kinase B/Akt signals impair Th17 differentiation and support natural regulatory T cell function and induced regulatory T cell formation. J Immunol. 2009;183:6124-34 pubmed publisher
Sanos S, Bui V, Mortha A, Oberle K, Heners C, Johner C, et al. RORgammat and commensal microflora are required for the differentiation of mucosal interleukin 22-producing NKp46+ cells. Nat Immunol. 2009;10:83-91 pubmed publisher
Ivanov I, McKenzie B, Zhou L, Tadokoro C, Lepelley A, Lafaille J, et al. The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells. Cell. 2006;126:1121-33 pubmed
Sun Z, Unutmaz D, Zou Y, Sunshine M, Pierani A, Brenner Morton S, et al. Requirement for RORgamma in thymocyte survival and lymphoid organ development. Science. 2000;288:2369-73 pubmed
He Y, Deftos M, Ojala E, Bevan M. RORgamma t, a novel isoform of an orphan receptor, negatively regulates Fas ligand expression and IL-2 production in T cells. Immunity. 1998;9:797-806 pubmed
Medvedev A, Yan Z, Hirose T, Giguere V, Jetten A. Cloning of a cDNA encoding the murine orphan receptor RZR/ROR gamma and characterization of its response element. Gene. 1996;181:199-206 pubmed
product information
Product Type :
Antibody
Product Name :
ROR gamma (t) Monoclonal Antibody (B2D), eBioscience
Catalog # :
14-6981-82
Quantity :
100 µg
Price :
US 514
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
Western Blot: 2-5 µg/mL
Species :
Mouse
Clone :
B2D
Isotype :
IgG1, kappa
Storage :
4° C
Description :
ROR gamma, a NR1 Thyroid Hormone-Like receptor, has been shown to affect thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. ROR gamma has been shown to promote thymocyte survival by activating the expression of the antiapoptotic protein Bcl-x(L). ROR gamma is also required for the development of lymph nodes and Peyer's patches. It has been shown that ROR gamma(t), a thymus-specific isoform of ROR gamma from mouse, inhibits Fas ligand expression and cytokine secretion in immature thymocytes. ROR gamma binds as a monomer to response elements composed of a single core motif, GGTCA, preceded by a 6 bp AT-rich sequence. ROR gamma expression has been documented in mouse thymus, adipose, bone, skeletal muscle, liver, and kidney, and in human skeletal muscle. ESTs have been isolated from human tissue libraries, including cancerous head/neck, germ cell, and prostate, and normal colon, head/neck, lung, liver/spleen, and pancreas. ROR gammat t (RORgt) is an isoform of RORC.
Format :
Liquid
Applications w/Dilutions :
Western Blot: 2-5 µg/mL
Aliases :
EGK_01272; IMD42; MGC129539; NR1F3; nuclear receptor ROR-gamma; Nuclear receptor RZR-gamma; nuclear receptor subfamily 1 group F member 3; RAR related orphan receptor C; RAR-related orphan nuclear receptor variant 2; RAR-related orphan receptor C; RAR-related orphan receptor C, isoform a; RAR-related orphan receptor gamma; retinoic acid-binding receptor gamma; retinoid-related orphan receptor gamma; retinoid-related orphan receptor-gamma; ROR Gamma; ROR gamma t; Rorc; Rorg; RORgamma; RORgt; RP11-98D18.11-001; RZRG; RZR-GAMMA; Thor; Thymus orphan receptor; TOR
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA