Zap-70 Monoclonal Antibody (1E7.2), eBioscience | Invitrogen 14-6695-82 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Zap-70 Monoclonal Antibody (1E7.2), eBioscience

catalog :

14-6695-82

quantity :

100 ug

price :

US 239.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1E7.2

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation, flow cytometry

more info or order :

Invitrogen product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; fig 6	Alsadeq A, Hobeika E, Medgyesi D, Kläsener K, Reth M. The role of the Syk/Shp-1 kinase-phosphatase equilibrium in B cell development and signaling. J Immunol. 2014;193:268-76 pubmed publisher
flow cytometry; mouse	Deswal S, Schulze A, Hofer T, Schamel W. Quantitative analysis of protein phosphorylations and interactions by multi-colour IP-FCM as an input for kinetic modelling of signalling networks. PLoS ONE. 2011;6:e22928 pubmed publisher
immunoprecipitation; mouse	Imada M, Masuda K, Satoh R, Ito Y, Goto Y, Matsuoka T, et al. Ectopically expressed PIR-B on T cells constitutively binds to MHC class I and attenuates T helper type 1 responses. Int Immunol. 2009;21:1151-61 pubmed publisher
flow cytometry; human	Preobrazhensky S, Bahler D. Optimization of flow cytometric measurement of ZAP-70 in chronic lymphocytic leukemia. Cytometry B Clin Cytom. 2008;74:118-27 pubmed
flow cytometry; human	Le Garff Tavernier M, Ticchioni M, Brissard M, Salmon C, Raynaud S, Davi F, et al. National standardization of ZAP-70 determination by flow cytometry: the French experience. Cytometry B Clin Cytom. 2007;72:103-8 pubmed
	González Pérez G, Lamousé Smith E. Gastrointestinal Microbiome Dysbiosis in Infant Mice Alters Peripheral CD8+ T Cell Receptor Signaling. Front Immunol. 2017;8:265 pubmed publisher
	Orchard J, Ibbotson R, Davis Z, Wiestner A, Rosenwald A, Thomas P, et al. ZAP-70 expression and prognosis in chronic lymphocytic leukaemia. Lancet. 2004;363:105-11 pubmed
	Chen L, Widhopf G, Huynh L, Rassenti L, Rai K, Weiss A, et al. Expression of ZAP-70 is associated with increased B-cell receptor signaling in chronic lymphocytic leukemia. Blood. 2002;100:4609-14 pubmed
	Qian D, Lev S, van Oers N, Dikic I, Schlessinger J, Weiss A. Tyrosine phosphorylation of Pyk2 is selectively regulated by Fyn during TCR signaling. J Exp Med. 1997;185:1253-9 pubmed
	Qian D, Mollenauer M, Weiss A. Dominant-negative zeta-associated protein 70 inhibits T cell antigen receptor signaling. J Exp Med. 1996;183:611-20 pubmed

product information

Product Type :

Antibody

Product Name :

Zap-70 Monoclonal Antibody (1E7.2), eBioscience

Catalog # :

14-6695-82

Quantity :

100 ug

Price :

US 239.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

Flow Cytometry: Assay-Dependent, Immunocytochemistry: 5 ug/mL, Immunoprecipitation: Assay-Dependent, Western Blot: 2 ug/mL

Species :

Human, Mouse

Clone :

1E7.2

Isotype :

IgG1, kappa

Storage :

4 C

Description :

ZAP-70 is a cytosolic protein tyrosine kinase that is a member of the Syk family of proteins. ZAP-70 is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor activation. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-Dependent, Immunocytochemistry: 5 ug/mL, Immunoprecipitation: Assay-Dependent, Western Blot: 2 ug/mL

Aliases :

70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; ADMIO2; EC 2.7.10.2; FLJ17670; FLJ17679; I79_004087; IMD48; kinase ZAP70; mrtle; mur; Selective T cell defect; Srk; STCD; STD; syk-related protein tyrosine kinase; syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; Tyrosine-protein kinase ZAP-70;TZK; TZK; ZA70; Zap70; ZAP-70; Zeta chain associated protein kinase 70kDa; zeta chain of T cell receptor associated protein kinase; zeta chain of T cell receptor associated protein kinase 70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase; zeta-chain (TCR) associated protein kinase 70; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD

more info or order :

Invitrogen product webpage