6x-His Tag Monoclonal Antibody (HIS.H8), eBioscience | Invitrogen 14-6657-80 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

6x-His Tag Monoclonal Antibody (HIS.H8), eBioscience

catalog :

14-6657-80

quantity :

25 ug

price :

US 172.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

HIS.H8

application :

western blot, immunocytochemistry, immunoprecipitation

citations: 24

Reference
Bridge T, Wegmann U, Crack J, Orman K, Shaikh S, Farndon W, et al. Site-specific encoding of photoactivity and photoreactivity into antibody fragments. Nat Chem Biol. 2023;19:740-749 pubmed publisher
Lei R, Hernandez Garcia A, Tan T, Teo Q, Wang Y, Zhang X, et al. Mutational fitness landscape of human influenza H3N2 neuraminidase. Cell Rep. 2023;42:111951 pubmed publisher
Ho H, Vo D, Lin T, Hung J, Chiu Y, Tsai M. Ganoderma microsporum immunomodulatory protein acts as a multifunctional broad-spectrum antiviral against SARS-CoV-2 by interfering virus binding to the host cells and spike-mediated cell fusion. Biomed Pharmacother. 2022;155:113766 pubmed publisher
Yeh H, Vo D, Lin Z, Ho H, Hua W, Qiu W, et al. GMI, a protein from Ganoderma microsporum, induces ACE2 degradation to alleviate infection of SARS-CoV-2 Spike-pseudotyped virus. Phytomedicine. 2022;103:154215 pubmed publisher
Vallecillo Z xfa niga M, Poulson P, Luddington J, Arnold C, Rathgeber M, Kartchner B, et al. Therapeutic Benefit of Galectin-1: Beyond Membrane Repair, a Multifaceted Approach to LGMD2B. Cells. 2021;10: pubmed publisher
Scheu A, Lim S, Metzner F, Mohammed S, Howarth M. NeissLock provides an inducible protein anhydride for covalent targeting of endogenous proteins. Nat Commun. 2021;12:717 pubmed publisher
Liu E, Macmillan C, Shafee T, Ma Y, Ratcliffe J, van de Meene A, et al. Fasciclin-Like Arabinogalactan-Protein 16 (FLA16) Is Required for Stem Development in Arabidopsis. Front Plant Sci. 2020;11:615392 pubmed publisher
Lewicka E, Mitura M, Steczkiewicz K, Kieracinska J, Skrzynska K, Adamczyk M, et al. Unique Properties of the Alpha-Helical DNA-Binding Protein KfrA Encoded by the IncU Incompatibility Group Plasmid RA3 and Its Host-Dependent Role in Plasmid Maintenance. Appl Environ Microbiol. 2021;87: pubmed publisher
Vallecillo Zúniga M, Rathgeber M, Poulson P, Hayes S, Luddington J, Gill H, et al. Treatment with galectin-1 improves myogenic potential and membrane repair in dysferlin-deficient models. PLoS ONE. 2020;15:e0238441 pubmed publisher
Roy A, Ghosh A, Kumar B, Chandran B. IFI16, a nuclear innate immune DNA sensor, mediates epigenetic silencing of herpesvirus genomes by its association with H3K9 methyltransferases SUV39H1 and GLP. elife. 2019;8: pubmed publisher
Shelke G, Yin Y, Jang S, Lässer C, Wennmalm S, Hoffmann H, et al. Endosomal signalling via exosome surface TGFβ-1. J Extracell Vesicles. 2019;8:1650458 pubmed publisher
Sager R, Woodford M, Backe S, Makedon A, Baker Williams A, DiGregorio B, et al. Post-translational Regulation of FNIP1 Creates a Rheostat for the Molecular Chaperone Hsp90. Cell Rep. 2019;26:1344-1356.e5 pubmed publisher
Rotoli S, Jones J, CARADONNA S. Cysteine residues contribute to the dimerization and enzymatic activity of human nuclear dUTP nucleotidohydrolase (nDut). Protein Sci. 2018;27:1797-1809 pubmed publisher
Pan H, Liu J, Deng W, Xing J, Li Q, Wang Z. Site-specific PEGylation of an anti-CEA/CD3 bispecific antibody improves its antitumor efficacy. Int J Nanomedicine. 2018;13:3189-3201 pubmed publisher
Lau S, Wong E, Tsang C, Ahmed S, Au Yeung R, Yuen K, et al. Discovery and Sequence Analysis of Four Deltacoronaviruses from Birds in the Middle East Reveal Interspecies Jumping with Recombination as a Potential Mechanism for Avian-to-Avian and Avian-to-Mammalian Transmission. J Virol. 2018;92: pubmed publisher
Yousefi S, Sharma S, Stojkov D, Germic N, Aeschlimann S, Ge M, et al. Oxidative damage of SP-D abolishes control of eosinophil extracellular DNA trap formation. J Leukoc Biol. 2018;104:205-214 pubmed publisher
Beck K, Hovhanyan A, Menegazzi P, Helfrich Forster C, Raabe T. Drosophila RSK Influences the Pace of the Circadian Clock by Negative Regulation of Protein Kinase Shaggy Activity. Front Mol Neurosci. 2018;11:122 pubmed publisher
Rayaprolu V, Royal P, Stengel K, Sandoz G, Kohout S. Dimerization of the voltage-sensing phosphatase controls its voltage-sensing and catalytic activity. J Gen Physiol. 2018;150:683-696 pubmed publisher
Besprozvannaya M, Dickson E, Li H, Ginburg K, Bers D, Auwerx J, et al. GRAM domain proteins specialize functionally distinct ER-PM contact sites in human cells. elife. 2018;7: pubmed publisher
Qin J, Wang W, Li X, Deokar H, Buolamwini J, Zhang R. Inhibiting ?-Catenin by ?-Carboline-Type MDM2 Inhibitor for Pancreatic Cancer Therapy. Front Pharmacol. 2018;9:5 pubmed publisher
Makins C, Ghosh S, Román Meléndez G, Malec P, Kennedy R, Marsh E. Does Viperin Function as a Radical S-Adenosyl-l-methionine-dependent Enzyme in Regulating Farnesylpyrophosphate Synthase Expression and Activity?. J Biol Chem. 2016;291:26806-26815 pubmed publisher
Knutson B, Smith M, Walker Kopp N, Xu X. Super elongation complex contains a TFIIF-related subcomplex. Transcription. 2016;7:133-40 pubmed publisher
Crowe J, Dobeli H, Gentz R, Hochuli E, Stuber D, Henco K. 6xHis-Ni-NTA chromatography as a superior technique in recombinant protein expression/purification. Methods Mol Biol. 1994;31:371-87 pubmed
Hengen P. Purification of His-Tag fusion proteins from Escherichia coli. Trends Biochem Sci. 1995;20:285-6 pubmed

product information

Product Type :

Antibody

Product Name :

6x-His Tag Monoclonal Antibody (HIS.H8), eBioscience

Catalog # :

14-6657-80

Quantity :

25 ug

Price :

US 172.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Tag

Applications :

Immunocytochemistry: 0.5 ug/mL, Immunoprecipitation: Assay-Dependent, Western Blot: 1 ug/mL

Species :

Tag

Clone :

HIS.H8

Isotype :

IgG2b

Storage :

4 C

Description :

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 0.5 ug/mL, Immunoprecipitation: Assay-Dependent, Western Blot: 1 ug/mL

Aliases :

6His tag; 6X His; His Tag; Histidine Tag