MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), eBioscience | Invitrogen 14-5999-85 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), eBioscience

catalog :

14-5999-85

quantity :

500 µg

price :

US 318

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

28-14-8

reactivity :

mouse

application :

flow cytometry

more info or order :

Invitrogen product webpage

citations: 32

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 4a	James K, Cosway E, LUCAS B, White A, Parnell S, Carvalho Gaspar M, et al. Endothelial cells act as gatekeepers for LTβR-dependent thymocyte emigration. J Exp Med. 2018;215:2984-2993 pubmed publisher
flow cytometry; mouse; 1:250; fig 3	Ludigs K, Jandus C, Utzschneider D, Staehli F, Bessoles S, Dang A, et al. NLRC5 shields T lymphocytes from NK-cell-mediated elimination under inflammatory conditions. Nat Commun. 2016;7:10554 pubmed publisher
flow cytometry; mouse; fig 3	Sun H, Zhang J, Chen F, Chen X, Zhou Z, Wang H. Activation of RAW264.7 macrophages by the polysaccharide from the roots of Actinidia eriantha and its molecular mechanisms. Carbohydr Polym. 2015;121:388-402 pubmed publisher
flow cytometry; mouse	Wei H, Nash W, Makrigiannis A, Brown M. Impaired NK-cell education diminishes resistance to murine CMV infection. Eur J Immunol. 2014;44:3273-82 pubmed publisher
flow cytometry; mouse; fig 4	Rauen J, Kreer C, Paillard A, van Duikeren S, Benckhuijsen W, Camps M, et al. Enhanced cross-presentation and improved CD8+ T cell responses after mannosylation of synthetic long peptides in mice. PLoS ONE. 2014;9:e103755 pubmed publisher
	Bekeschus S, Clemen R, Nießner F, Sagwal S, Freund E, Schmidt A. Medical Gas Plasma Jet Technology Targets Murine Melanoma in an Immunogenic Fashion. Adv Sci (Weinh). 2020;7:1903438 pubmed publisher
	Westrich J, Vermeer D, Silva A, Bonney S, Berger J, Cicchini L, et al. CXCL14 suppresses human papillomavirus-associated head and neck cancer through antigen-specific CD8+ T-cell responses by upregulating MHC-I expression. Oncogene. 2019;: pubmed publisher
	Buxade M, Huerga Encabo H, Riera Borrull M, Quintana Gallardo L, López Cotarelo P, Tellechea M, et al. Macrophage-specific MHCII expression is regulated by a remote Ciita enhancer controlled by NFAT5. J Exp Med. 2018;215:2901-2918 pubmed publisher
	Murakami G, Edamura M, Furukawa T, Kawasaki H, Kosugi I, Fukuda A, et al. MHC class I in dopaminergic neurons suppresses relapse to reward seeking. Sci Adv. 2018;4:eaap7388 pubmed publisher
	Ahn R, Sabourin V, Bolt A, Hebert S, Totten S, De Jay N, et al. The Shc1 adaptor simultaneously balances Stat1 and Stat3 activity to promote breast cancer immune suppression. Nat Commun. 2017;8:14638 pubmed publisher
	Selby M, Engelhardt J, Johnston R, Lu L, Han M, Thudium K, et al. Preclinical Development of Ipilimumab and Nivolumab Combination Immunotherapy: Mouse Tumor Models, In Vitro Functional Studies, and Cynomolgus Macaque Toxicology. PLoS ONE. 2016;11:e0161779 pubmed publisher
	Biswas A, Bruder D, Wolf S, Jeron A, Mack M, Heimesaat M, et al. Ly6C(high) monocytes control cerebral toxoplasmosis. J Immunol. 2015;194:3223-35 pubmed publisher
	Qin Y, Zhou P, Zhou C, Li J, Gao W. The adipose-derived lineage-negative cells are enriched mesenchymal stem cells and promote limb ischemia recovery in mice. Stem Cells Dev. 2014;23:363-71 pubmed publisher
	Brentville V, Metheringham R, Gunn B, Durrant L. High avidity cytotoxic T lymphocytes can be selected into the memory pool but they are exquisitely sensitive to functional impairment. PLoS ONE. 2012;7:e41112 pubmed publisher
	Cho H, Reyes Vargas E, Delgado J, Celis E. A potent vaccination strategy that circumvents lymphodepletion for effective antitumor adoptive T-cell therapy. Cancer Res. 2012;72:1986-95 pubmed publisher
	Nouri Shirazi M, Guinet E. Exposure to nicotine adversely affects the dendritic cell system and compromises host response to vaccination. J Immunol. 2012;188:2359-70 pubmed publisher
	Schmidt R, Filak H, Lemon J, Potter T, Lenz L. A LysM and SH3-domain containing region of the Listeria monocytogenes p60 protein stimulates accessory cells to promote activation of host NK cells. PLoS Pathog. 2011;7:e1002368 pubmed publisher
	Nice T, Deng W, Coscoy L, Raulet D. Stress-regulated targeting of the NKG2D ligand Mult1 by a membrane-associated RING-CH family E3 ligase. J Immunol. 2010;185:5369-76 pubmed publisher
	Eweda G, Suzuki D, Nagata T, Tsujimura K, Koide Y. Identification of murine T-cell epitopes on low-molecular-mass secretory proteins (CFP11, CFP17, and TB18.5) of Mycobacterium tuberculosis. Vaccine. 2010;28:4616-25 pubmed publisher
	Suzuki D, Nagata T, Eweda G, Matsumoto S, Matsumoto M, Tsujimura K, et al. Characterization of murine T-cell epitopes on mycobacterial DNA-binding protein 1 (MDP1) using DNA vaccination. Vaccine. 2010;28:2020-5 pubmed publisher
	Zeng M, Guinet E, Nouri Shirazi M. Comparative analysis of dendritic cells and anti-CD3/CD28 expanded regulatory T cells for application in transplantation. Transpl Immunol. 2009;22:82-92 pubmed publisher
	Phares T, Ramakrishna C, Parra G, Epstein A, Chen L, Atkinson R, et al. Target-dependent B7-H1 regulation contributes to clearance of central nervous system infection and dampens morbidity. J Immunol. 2009;182:5430-8 pubmed publisher
	Panchal R, Ulrich R, Bradfute S, Lane D, Ruthel G, Kenny T, et al. Reduced expression of CD45 protein-tyrosine phosphatase provides protection against anthrax pathogenesis. J Biol Chem. 2009;284:12874-85 pubmed publisher
	Frenzel L, Abdullah Z, Kriegeskorte A, Dieterich R, Lange N, Busch D, et al. Role of natural-killer group 2 member D ligands and intercellular adhesion molecule 1 in natural killer cell-mediated lysis of murine embryonic stem cells and embryonic stem cell-derived cardiomyocytes. Stem Cells. 2009;27:307-16 pubmed publisher
	Rifa i M, Shi Z, Zhang S, Lee Y, Shiku H, Isobe K, et al. CD8+CD122+ regulatory T cells recognize activated T cells via conventional MHC class I-alphabetaTCR interaction and become IL-10-producing active regulatory cells. Int Immunol. 2008;20:937-47 pubmed publisher
	Malone K, Stohlman S, Ramakrishna C, Macklin W, Bergmann C. Induction of class I antigen processing components in oligodendroglia and microglia during viral encephalomyelitis. Glia. 2008;56:426-35 pubmed publisher
	Kuwano Y, Prazma C, Yazawa N, Watanabe R, Ishiura N, Kumanogoh A, et al. CD83 influences cell-surface MHC class II expression on B cells and other antigen-presenting cells. Int Immunol. 2007;19:977-92 pubmed
	Xie X, Dighe A, Clark P, Sabastian P, Buss S, Brown M. Deficient major histocompatibility complex-linked innate murine cytomegalovirus immunity in MA/My.L-H2b mice and viral downregulation of H-2k class I proteins. J Virol. 2007;81:229-36 pubmed
	Kemball C, Lee E, Vezys V, Pearson T, Larsen C, Lukacher A. Late priming and variability of epitope-specific CD8+ T cell responses during a persistent virus infection. J Immunol. 2005;174:7950-60 pubmed
	Blattman J, Antia R, Sourdive D, Wang X, Kaech S, Murali Krishna K, et al. Estimating the precursor frequency of naive antigen-specific CD8 T cells. J Exp Med. 2002;195:657-64 pubmed
	Ozato K, Hansen T, Sachs D. Monoclonal antibodies to mouse MHC antigens. II. Antibodies to the H-2Ld antigen, the products of a third polymorphic locus of the mouse major histocompatibility complex. J Immunol. 1980;125:2473-7 pubmed
	Ozato K, Mayer N, Sachs D. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol. 1980;124:533-40 pubmed

product information

Product Type :

Antibody

Product Name :

MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), eBioscience

Catalog # :

14-5999-85

Quantity :

500 µg

Price :

US 318

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Mouse

Applications :

Flow Cytometry: 1 µg/test

Species :

Mouse

Clone :

28-14-8

Isotype :

IgG2a, kappa

Storage :

4° C

Description :

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1 µg/test

Aliases :

H-2 class I histocompatibility antigen, D-B alpha chain; H-2D; H2-D; H-2D cell surface glycoprotein; H-2D(B); H2D1; H2-D1; H2DB; H2-DC1-beta transplantation antigen protein; H2L; H2LD; histocompatibility 2, D region locus 1; MHC class I antigen; MHC class I H2 antigen; MHC class I H-2Dp; MHC H2-D-q alpha-chain; Q5k protein

more info or order :

Invitrogen product webpage