CD273 (B7-DC) Monoclonal Antibody (TY25), eBioscience | Invitrogen 14-5986-81 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD273 (B7-DC) Monoclonal Antibody (TY25), eBioscience

catalog :

14-5986-81

quantity :

50 µg

price :

US 132.00

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

TY25

reactivity :

mouse

application :

western blot, immunohistochemistry, neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 75

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 1b	Mizuno R, Sugiura D, Shimizu K, Maruhashi T, Watada M, Okazaki I, et al. PD-1 Primarily Targets TCR Signal in the Inhibition of Functional T Cell Activation. Front Immunol. 2019;10:630 pubmed publisher
flow cytometry; mouse; loading ...; fig 1b	Vander Lugt B, Riddell J, Khan A, Hackney J, Lesch J, DeVoss J, et al. Transcriptional determinants of tolerogenic and immunogenic states during dendritic cell maturation. J Cell Biol. 2017;216:779-792 pubmed publisher
flow cytometry; mouse; fig 3	Hartung E, Becker M, Bachem A, Reeg N, JÃ¤kel A, Hutloff A, et al. Induction of potent CD8 T cell cytotoxicity by specific targeting of antigen to cross-presenting dendritic cells in vivo via murine or human XCR1. J Immunol. 2015;194:1069-79 pubmed publisher
flow cytometry; mouse; fig 3	Bodhankar S, Chen Y, Vandenbark A, Murphy S, Offner H. PD-L1 enhances CNS inflammation and infarct volume following experimental stroke in mice in opposition to PD-1. J Neuroinflammation. 2013;10:111 pubmed publisher
flow cytometry; mouse; fig s2	Van Der Werf N, Redpath S, Azuma M, Yagita H, Taylor M. Th2 cell-intrinsic hypo-responsiveness determines susceptibility to helminth infection. PLoS Pathog. 2013;9:e1003215 pubmed publisher
flow cytometry; mouse	Tasso R, Augello A, Boccardo S, Salvi S, Carida M, Postiglione F, et al. Recruitment of a host's osteoprogenitor cells using exogenous mesenchymal stem cells seeded on porous ceramic. Tissue Eng Part A. 2009;15:2203-12 pubmed publisher
flow cytometry; mouse	Sheng H, Wang Y, Jin Y, Zhang Q, Zhang Y, Wang L, et al. A critical role of IFNgamma in priming MSC-mediated suppression of T cell proliferation through up-regulation of B7-H1. Cell Res. 2008;18:846-57 pubmed publisher
flow cytometry; mouse; fig 1	Nakae S, Iwakura Y, Suto H, Galli S. Phenotypic differences between Th1 and Th17 cells and negative regulation of Th1 cell differentiation by IL-17. J Leukoc Biol. 2007;81:1258-68 pubmed
flow cytometry; mouse	Kuipers H, Muskens F, Willart M, Hijdra D, van Assema F, Coyle A, et al. Contribution of the PD-1 ligands/PD-1 signaling pathway to dendritic cell-mediated CD4+ T cell activation. Eur J Immunol. 2006;36:2472-82 pubmed
flow cytometry; mouse; fig 1	Shin T, Yoshimura K, Shin T, Crafton E, Tsuchiya H, Housseau F, et al. In vivo costimulatory role of B7-DC in tuning T helper cell 1 and cytotoxic T lymphocyte responses. J Exp Med. 2005;201:1531-41 pubmed
immunohistochemistry - frozen section; mouse flow cytometry; mouse	Schoop R, Wahl P, Le Hir M, Heemann U, Wang M, Wuthrich R. Suppressed T-cell activation by IFN-gamma-induced expression of PD-L1 on renal tubular epithelial cells. Nephrol Dial Transplant. 2004;19:2713-20 pubmed
blocking or activating experiments; mouse flow cytometry; mouse	Smith P, Walsh C, Mangan N, Fallon R, Sayers J, McKenzie A, et al. Schistosoma mansoni worms induce anergy of T cells via selective up-regulation of programmed death ligand 1 on macrophages. J Immunol. 2004;173:1240-8 pubmed
	Liu Y, Gao Y, Hao H, Hou T. CD279 mediates the homeostasis and survival of regulatory T cells by enhancing T cell and macrophage interactions. FEBS Open Bio. 2020;10:1162-1170 pubmed publisher
	Prasad S, Hu S, Sheng W, Chauhan P, Lokensgard J. Reactive glia promote development of CD103+ CD69+ CD8+ T-cells through programmed cell death-ligand 1 (PD-L1). Immun Inflamm Dis. 2018;6:332-344 pubmed publisher
	Uto T, Takagi H, Fukaya T, Nasu J, Fukui T, Miyanaga N, et al. Critical role of plasmacytoid dendritic cells in induction of oral tolerance. J Allergy Clin Immunol. 2018;141:2156-2167.e9 pubmed publisher
	Shiokawa A, Kotaki R, Takano T, Nakajima Adachi H, Hachimura S. Mesenteric lymph node CD11b- CD103+ PD-L1High dendritic cells highly induce regulatory T cells. Immunology. 2017;152:52-64 pubmed publisher
	Oh J, Oh D, Jung H, Lee H. A mechanism for the induction of type 2 immune responses by a protease allergen in the genital tract. Proc Natl Acad Sci U S A. 2017;114:E1188-E1195 pubmed publisher
	Laidlaw B, Cui W, Amezquita R, Gray S, Guan T, Lu Y, et al. Production of IL-10 by CD4(+) regulatory T cells during the resolution of infection promotes the maturation of memory CD8(+) T cells. Nat Immunol. 2015;16:871-9 pubmed publisher
	Hong Y, Manoharan I, Suryawanshi A, Majumdar T, Angus Hill M, Koni P, et al. Î²-catenin promotes regulatory T-cell responses in tumors by inducing vitamin A metabolism in dendritic cells. Cancer Res. 2015;75:656-665 pubmed publisher
	Bodhankar S, Chen Y, Lapato A, Vandenbark A, Murphy S, Offner H. Targeting immune co-stimulatory effects of PD-L1 and PD-L2 might represent an effective therapeutic strategy in stroke. Front Cell Neurosci. 2014;8:228 pubmed publisher
	Huang S, Everts B, Ivanova Y, O Sullivan D, Nascimento M, Smith A, et al. Cell-intrinsic lysosomal lipolysis is essential for alternative activation of macrophages. Nat Immunol. 2014;15:846-55 pubmed publisher
	Schachtele S, Hu S, Sheng W, Mutnal M, Lokensgard J. Glial cells suppress postencephalitic CD8+ T lymphocytes through PD-L1. Glia. 2014;62:1582-94 pubmed publisher
	Zhang X, Lund H, Mia S, Parsa R, Harris R. Adoptive transfer of cytokine-induced immunomodulatory adult microglia attenuates experimental autoimmune encephalomyelitis in DBA/1 mice. Glia. 2014;62:804-17 pubmed
	Huang X, Chen Y, Chung C, Yuan Z, Monaghan S, Wang F, et al. Identification of B7-H1 as a novel mediator of the innate immune/proinflammatory response as well as a possible myeloid cell prognostic biomarker in sepsis. J Immunol. 2014;192:1091-9 pubmed publisher
	Kroesen M, Nierkens S, Ansems M, Wassink M, Orentas R, Boon L, et al. A transplantable TH-MYCN transgenic tumor model in C57Bl/6 mice for preclinical immunological studies in neuroblastoma. Int J Cancer. 2014;134:1335-45 pubmed publisher
	Bonne Annee S, Kerepesi L, Hess J, O Connell A, Lok J, Nolan T, et al. Human and mouse macrophages collaborate with neutrophils to kill larval Strongyloides stercoralis. Infect Immun. 2013;81:3346-55 pubmed publisher
	Cascio J, Haymaker C, Divekar R, Zaghouani S, Khairallah M, Wan X, et al. Antigen-specific effector CD4 T lymphocytes school lamina propria dendritic cells to transfer innate tolerance. J Immunol. 2013;190:6004-14 pubmed publisher
	el Bannoudi H, Han W, Stoop J, Louis Plence P, Huizinga T, Toes R. DX5+ CD4+ T cells modulate CD4+ T-cell response via inhibition of IL-12 production by DCs. Eur J Immunol. 2013;43:439-46 pubmed publisher
	Lewkowich I, Lajoie S, Stoffers S, Suzuki Y, Richgels P, Dienger K, et al. PD-L2 modulates asthma severity by directly decreasing dendritic cell IL-12 production. Mucosal Immunol. 2013;6:728-39 pubmed publisher
	Tewalt E, Cohen J, Rouhani S, Guidi C, Qiao H, Fahl S, et al. Lymphatic endothelial cells induce tolerance via PD-L1 and lack of costimulation leading to high-level PD-1 expression on CD8 T cells. Blood. 2012;120:4772-82 pubmed publisher
	Parsa R, Andresen P, Gillett A, Mia S, Zhang X, Mayans S, et al. Adoptive transfer of immunomodulatory M2 macrophages prevents type 1 diabetes in NOD mice. Diabetes. 2012;61:2881-92 pubmed publisher
	Jiang H, Milovanovic M, Allan D, Niedbala W, Besnard A, Fukada S, et al. IL-33 attenuates EAE by suppressing IL-17 and IFN-? production and inducing alternatively activated macrophages. Eur J Immunol. 2012;42:1804-14 pubmed publisher
	Wythe S, Dodd J, Openshaw P, Schwarze J. OX40 ligand and programmed cell death 1 ligand 2 expression on inflammatory dendritic cells regulates CD4 T cell cytokine production in the lung during viral disease. J Immunol. 2012;188:1647-55 pubmed publisher
	Ren X, Akiyoshi K, Vandenbark A, Hurn P, Offner H. Programmed death-1 pathway limits central nervous system inflammation and neurologic deficits in murine experimental stroke. Stroke. 2011;42:2578-83 pubmed publisher
	Zhong X, Rothstein T. L2pB1: a new player in autoimmunity. Mol Immunol. 2011;48:1292-300 pubmed publisher
	Tomayko M, Steinel N, Anderson S, Shlomchik M. Cutting edge: Hierarchy of maturity of murine memory B cell subsets. J Immunol. 2010;185:7146-50 pubmed publisher
	Sakai S, Kawamura I, Okazaki T, Tsuchiya K, Uchiyama R, Mitsuyama M. PD-1-PD-L1 pathway impairs T(h)1 immune response in the late stage of infection with Mycobacterium bovis bacillus Calmette-Guérin. Int Immunol. 2010;22:915-25 pubmed publisher
	Otani T, Tsuji Takayama K, Okochi A, Yamamoto M, Takeuchi M, Yamasaki F, et al. Stromal cells' B7-1 is a key stimulatory molecule for interleukin-10 production by HOZOT, a multifunctional regulatory T-cell line. Immunol Cell Biol. 2011;89:246-54 pubmed publisher
	Fukaya T, Takagi H, Sato Y, Sato K, Eizumi K, Taya H, et al. Crucial roles of B7-H1 and B7-DC expressed on mesenteric lymph node dendritic cells in the generation of antigen-specific CD4+Foxp3+ regulatory T cells in the establishment of oral tolerance. Blood. 2010;116:2266-76 pubmed publisher
	Soudja S, Wehbe M, Mas A, Chasson L, de Tenbossche C, Huijbers I, et al. Tumor-initiated inflammation overrides protective adaptive immunity in an induced melanoma model in mice. Cancer Res. 2010;70:3515-25 pubmed publisher
	Guerrero A, Jain N, Wang X, Fries B. Cryptococcus neoformans variants generated by phenotypic switching differ in virulence through effects on macrophage activation. Infect Immun. 2010;78:1049-57 pubmed publisher
	Akbari O, Stock P, Singh A, Lombardi V, Lee W, Freeman G, et al. PD-L1 and PD-L2 modulate airway inflammation and iNKT-cell-dependent airway hyperreactivity in opposing directions. Mucosal Immunol. 2010;3:81-91 pubmed publisher
	Davies J, Sheil B, Shanahan F. Bacterial signalling overrides cytokine signalling and modifies dendritic cell differentiation. Immunology. 2009;128:e805-15 pubmed publisher
	Okudaira K, Hokari R, Tsuzuki Y, Okada Y, Komoto S, Watanabe C, et al. Blockade of B7-H1 or B7-DC induces an anti-tumor effect in a mouse pancreatic cancer model. Int J Oncol. 2009;35:741-9 pubmed
	Phares T, Ramakrishna C, Parra G, Epstein A, Chen L, Atkinson R, et al. Target-dependent B7-H1 regulation contributes to clearance of central nervous system infection and dampens morbidity. J Immunol. 2009;182:5430-8 pubmed publisher
	Chen L, Pai V, Levinson R, Sharpe A, Freeman G, Braun J, et al. Constitutive neuronal expression of the immune regulator, programmed death 1 (PD-1), identified during experimental autoimmune uveitis. Ocul Immunol Inflamm. 2009;17:47-55 pubmed publisher
	Boehme S, Chen E, Franz Bacon K, Sasik R, Sprague L, Ly T, et al. Antagonism of CRTH2 ameliorates chronic epicutaneous sensitization-induced inflammation by multiple mechanisms. Int Immunol. 2009;21:1-17 pubmed publisher
	Schreiner B, Bailey S, Shin T, Chen L, Miller S. PD-1 ligands expressed on myeloid-derived APC in the CNS regulate T-cell responses in EAE. Eur J Immunol. 2008;38:2706-17 pubmed publisher
	Siracusa M, Reece J, Urban J, Scott A. Dynamics of lung macrophage activation in response to helminth infection. J Leukoc Biol. 2008;84:1422-33 pubmed publisher
	Menke J, Lucas J, Zeller G, Keir M, Huang X, Tsuboi N, et al. Programmed death 1 ligand (PD-L) 1 and PD-L2 limit autoimmune kidney disease: distinct roles. J Immunol. 2007;179:7466-77 pubmed
	Habicht A, Kewalaramani R, Vu M, Demirci G, Blazar B, Sayegh M, et al. Striking dichotomy of PD-L1 and PD-L2 pathways in regulating alloreactive CD4(+) and CD8(+) T cells in vivo. Am J Transplant. 2007;7:2683-92 pubmed
	Tanaka K, Albin M, Yuan X, Yamaura K, Habicht A, Murayama T, et al. PDL1 is required for peripheral transplantation tolerance and protection from chronic allograft rejection. J Immunol. 2007;179:5204-10 pubmed
	Shen L, Jin Y, Freeman G, Sharpe A, Dana M. The function of donor versus recipient programmed death-ligand 1 in corneal allograft survival. J Immunol. 2007;179:3672-9 pubmed
	Priatel J, Chen X, Zenewicz L, Shen H, Harder K, Horwitz M, et al. Chronic immunodeficiency in mice lacking RasGRP1 results in CD4 T cell immune activation and exhaustion. J Immunol. 2007;179:2143-52 pubmed
	Terawaki S, Tanaka Y, Nagakura T, Hayashi T, Shibayama S, Muroi K, et al. Specific and high-affinity binding of tetramerized PD-L1 extracellular domain to PD-1-expressing cells: possible application to enhance T cell function. Int Immunol. 2007;19:881-90 pubmed
	Shu S, Lian Z, Chuang Y, Yang G, Moritoki Y, Comstock S, et al. The role of CD11c(+) hepatic dendritic cells in the induction of innate immune responses. Clin Exp Immunol. 2007;149:335-43 pubmed
	Carter L, Leach M, Azoitei M, Cui J, Pelker J, Jussif J, et al. PD-1/PD-L1, but not PD-1/PD-L2, interactions regulate the severity of experimental autoimmune encephalomyelitis. J Neuroimmunol. 2007;182:124-34 pubmed
	Mintern J, Klemm E, Wagner M, Paquet M, Napier M, Kim Y, et al. Viral interference with B7-1 costimulation: a new role for murine cytomegalovirus fc receptor-1. J Immunol. 2006;177:8422-31 pubmed
	Fife B, Guleria I, Gubbels Bupp M, Eagar T, Tang Q, Bour Jordan H, et al. Insulin-induced remission in new-onset NOD mice is maintained by the PD-1-PD-L1 pathway. J Exp Med. 2006;203:2737-47 pubmed
	Geng L, Jiang G, Xie H, Fang Y, Dong S, Chen Y, et al. Mycophenolic acid upregulates B7-DC expression on dendritic cells, which is associated with impaired allostimulatory capacity of dendritic cells. Transplant Proc. 2006;38:1622-4 pubmed
	Nakae S, Suto H, Iikura M, Kakurai M, Sedgwick J, Tsai M, et al. Mast cells enhance T cell activation: importance of mast cell costimulatory molecules and secreted TNF. J Immunol. 2006;176:2238-48 pubmed
	Hessel E, Chu M, Lizcano J, Chang B, Herman N, Kell S, et al. Immunostimulatory oligonucleotides block allergic airway inflammation by inhibiting Th2 cell activation and IgE-mediated cytokine induction. J Exp Med. 2005;202:1563-73 pubmed
	Stagg J, Pommey S, Eliopoulos N, Galipeau J. Interferon-gamma-stimulated marrow stromal cells: a new type of nonhematopoietic antigen-presenting cell. Blood. 2006;107:2570-7 pubmed
	Rizzitelli A, Vremec D, Villadangos J, Mavaddat N, Wright M, Shortman K. Switching from a restricted to an effective CD4 T cell response by activating CD8+ murine dendritic cells with a Toll-like receptor 9 ligand. Eur J Immunol. 2005;35:3209-20 pubmed
	Terrazas L, Montero D, Terrazas C, Reyes J, Rodriguez Sosa M. Role of the programmed Death-1 pathway in the suppressive activity of alternatively activated macrophages in experimental cysticercosis. Int J Parasitol. 2005;35:1349-58 pubmed
	Augello A, Tasso R, Negrini S, Amateis A, Indiveri F, Cancedda R, et al. Bone marrow mesenchymal progenitor cells inhibit lymphocyte proliferation by activation of the programmed death 1 pathway. Eur J Immunol. 2005;35:1482-90 pubmed
	De Creus A, Abe M, Lau A, Hackstein H, Raimondi G, Thomson A. Low TLR4 expression by liver dendritic cells correlates with reduced capacity to activate allogeneic T cells in response to endotoxin. J Immunol. 2005;174:2037-45 pubmed
	Aramaki O, Shirasugi N, Takayama T, Shimazu M, Kitajima M, Ikeda Y, et al. Programmed death-1-programmed death-L1 interaction is essential for induction of regulatory cells by intratracheal delivery of alloantigen. Transplantation. 2004;77:6-12 pubmed
	Kanai T, Totsuka T, Uraushihara K, Makita S, Nakamura T, Koganei K, et al. Blockade of B7-H1 suppresses the development of chronic intestinal inflammation. J Immunol. 2003;171:4156-63 pubmed
	Ansari M, Salama A, Chitnis T, Smith R, Yagita H, Akiba H, et al. The programmed death-1 (PD-1) pathway regulates autoimmune diabetes in nonobese diabetic (NOD) mice. J Exp Med. 2003;198:63-9 pubmed
	Liu X, Gao J, Wen J, Yin L, Li O, Zuo T, et al. B7DC/PDL2 promotes tumor immunity by a PD-1-independent mechanism. J Exp Med. 2003;197:1721-30 pubmed
	Yamazaki T, Akiba H, Iwai H, Matsuda H, Aoki M, Tanno Y, et al. Expression of programmed death 1 ligands by murine T cells and APC. J Immunol. 2002;169:5538-45 pubmed
	Carter L, Fouser L, Jussif J, Fitz L, Deng B, Wood C, et al. PD-1:PD-L inhibitory pathway affects both CD4(+) and CD8(+) T cells and is overcome by IL-2. Eur J Immunol. 2002;32:634-43 pubmed
	Tseng S, Otsuji M, Gorski K, Huang X, Slansky J, Pai S, et al. B7-DC, a new dendritic cell molecule with potent costimulatory properties for T cells. J Exp Med. 2001;193:839-46 pubmed
	Latchman Y, Wood C, Chernova T, Chaudhary D, Borde M, Chernova I, et al. PD-L2 is a second ligand for PD-1 and inhibits T cell activation. Nat Immunol. 2001;2:261-8 pubmed

product information

Product Type :

Antibody

Product Name :

CD273 (B7-DC) Monoclonal Antibody (TY25), eBioscience

Catalog # :

14-5986-81

Quantity :

50 µg

Price :

US 132.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Rat

Reactivity :

Mouse

Applications :

Flow Cytometry: 0.5 µg/test, Functional Assay: Assay-Dependent, Immunohistochemistry (Frozen): Assay-Dependent, Immunoprecipitation: Assay-Dependent, Neutralization: Assay-Dependent, Western Blot: Assay-Dependent

Species :

Mouse

Clone :

TY25

Isotype :

IgG2a, kappa

Storage :

4° C

Description :

Programmed death-ligand 2 (PD-L2), or B7-DC, is a member of the B7 ligand family within the immunoglobulin superfamily that, along with programmed death-ligand 1 (PD-L1), acts as a ligand for programmed cell death protein 1 (PD-1). Though expressed primarily in dendritic cells, PD-L2 expression can be induced on a wide variety of immune and non-immune cells depending on the microenvironment. PD-L2 expression is particularly upregulated in the presence of Th2 cytokine, IL-4, as well as Th1 cytokines, TNF-alpha and IFN-gamma to a lesser degree. While generally expressed at lower levels compared to PD-L1, PD-L2 demonstrates a 2 to 6 times higher relative affinity to PD-1 than PD-L1. PD-1 and its ligands are referred to as inhibitory immune checkpoint molecules in that they provide useful negative feedback during physiological homeostasis. Ligation of PD-L2 or PD-L1 inhibits activation, proliferation, and cytokine secretion (e.g. IFN-gamma, IL-10) in T cells, ultimately dampening immune response. Conversely, studies have shown that PD-L2 can also stimulate T cell proliferation and cytokine production, even in PD-1-deficient T cells, suggesting additional receptors. Recent studies have concluded that PD-L2 also binds to a second receptor, repulsive guidance molecule b (RGMb), which was originally identified as a receptor for bone morphogenetic proteins (BMPs). RGMb is expressed in the central nervous system, as well as in macrophages, however, its role in immunity is only beginning to emerge. Interaction between PD-L2 and RGMb regulates the development of respiratory tolerance in the lung through BMP and/or neogenin signaling pathways. The naturally occurring human PD-L2 monomer consists of a 201-amino-acid extracellular domain, a 21-amino-acid transmembrane domain, and a 32-amino-acid cytoplasmic domain.

Format :

Liquid

Applications w/Dilutions :

Aliases :

B7 dendritic cell molecule; B7DC; B7-DC; bA574F11.2; Btdc; Butyrophilin B7-DC; butyrophilin-like protein; CD273; F730015O22Rik; MGC124039; MGC124040; PD1 ligand 2; PD-1 ligand 2; PD-1-ligand 2; PDCD1 ligand 2; PDCD1L2; Pdcd1lg2; Pdl2; PD-L2; Programmed cell death 1 ligand 2; programmed death ligand 2

more info or order :

Invitrogen product webpage