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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
AID Monoclonal Antibody (mAID-2), eBioscience
catalog :
14-5959-82
quantity :
100 µg
price :
US 450.00
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
mAID-2
reactivity :
human, mouse
application :
western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, western blot knockout validation
more info or order :
Invitrogen product webpage
citations: 28
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; fig S2
Yousif A, Stanlie A, Mondal S, Honjo T, Begum N. Differential regulation of S-region hypermutation and class-switch recombination by noncanonical functions of uracil DNA glycosylase. Proc Natl Acad Sci U S A. 2014;111:E1016-24 pubmed publisher
  • western blot; mouse; loading ...; fig 1a
Compagno M, Wang Q, Pighi C, Cheong T, Meng F, Poggio T, et al. Phosphatidylinositol 3-kinase δ blockade increases genomic instability in B cells. Nature. 2017;542:489-493 pubmed publisher
  • western blot; human; fig 2b
Kalchschmidt J, Bashford Rogers R, Paschos K, Gillman A, Styles C, Kellam P, et al. Epstein-Barr virus nuclear protein EBNA3C directly induces expression of AID and somatic mutations in B cells. J Exp Med. 2016;213:921-8 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1c
Jackson S, Jacobs H, Arkatkar T, Dam E, Scharping N, Kolhatkar N, et al. B cell IFN-γ receptor signaling promotes autoimmune germinal centers via cell-intrinsic induction of BCL-6. J Exp Med. 2016;213:733-50 pubmed publisher
  • immunohistochemistry - frozen section; mouse
Wang J, New J, Xie S, Yang P, Wu Q, Li J, et al. Extension of the germinal center stage of B cell development promotes autoantibodies in BXD2 mice. Arthritis Rheum. 2013;65:2703-12 pubmed publisher
Bai W, Zhu G, Xu J, Chen P, Meng F, Xue H, et al. The 3'-flap endonuclease XPF-ERCC1 promotes alternative end joining and chromosomal translocation during B cell class switching. Cell Rep. 2021;36:109756 pubmed publisher
Sun X, Liu M, Bai J, Xu J, Zhu C, Dong J, et al. ATR kinase activity promotes antibody class switch recombination in B cells through cell cycle regulation without suppressing DSB resection and microhomology usage. J Leukoc Biol. 2021;110:1101-1112 pubmed publisher
Iizuka T, Wakae K, Ono M, Suzuki T, Mizumoto Y, Kitamura K, et al. Activation-induced cytidine deaminase is a possible regulator of cross-talk between oocytes and granulosa cells through GDF-9 and SCF feedback system. Sci Rep. 2021;11:3833 pubmed publisher
Delgado P, xc1 lvarez Prado x, Marina Z xe1 rate E, Sernández I, Mur S, de la Barrera J, et al. Interplay between UNG and AID governs intratumoral heterogeneity in mature B cell lymphoma. PLoS Genet. 2020;16:e1008960 pubmed publisher
Delgado Benito V, Berruezo Llacuna M, Altwasser R, Winkler W, Sundaravinayagam D, Balasubramanian S, et al. PDGFA-associated protein 1 protects mature B lymphocytes from stress-induced cell death and promotes antibody gene diversification. J Exp Med. 2020;217: pubmed publisher
Kim A, Han L, Yu K. Immunoglobulin Class Switch Recombination is initiated by rare cytosine deamination events at switch regions. Mol Cell Biol. 2020;: pubmed publisher
Trivedi N, Weisel F, Smita S, Joachim S, Kader M, Radhakrishnan A, et al. Liver Is a Generative Site for the B Cell Response to Ehrlichia muris. Immunity. 2019;51:1088-1101.e5 pubmed publisher
Methot S, Litzler L, Subramani P, Eranki A, Fifield H, Patenaude A, et al. A licensing step links AID to transcription elongation for mutagenesis in B cells. Nat Commun. 2018;9:1248 pubmed publisher
Marina Zárate E, Pérez García A, Ramiro A. CCCTC-Binding Factor Locks Premature IgH Germline Transcription and Restrains Class Switch Recombination. Front Immunol. 2017;8:1076 pubmed publisher
Inoue T, Shinnakasu R, Ise W, Kawai C, Egawa T, Kurosaki T. The transcription factor Foxo1 controls germinal center B cell proliferation in response to T cell help. J Exp Med. 2017;214:1181-1198 pubmed publisher
Fabbri G, Holmes A, Viganotti M, Scuoppo C, Belver L, Herranz D, et al. Common nonmutational NOTCH1 activation in chronic lymphocytic leukemia. Proc Natl Acad Sci U S A. 2017;114:E2911-E2919 pubmed publisher
Wang Q, Kieffer Kwon K, Oliveira T, Mayer C, Yao K, Pai J, et al. The cell cycle restricts activation-induced cytidine deaminase activity to early G1. J Exp Med. 2017;214:49-58 pubmed publisher
Montamat Sicotte D, Litzler L, Abreu C, Safavi S, Zahn A, Orthwein A, et al. HSP90 inhibitors decrease AID levels and activity in mice and in human cells. Eur J Immunol. 2015;45:2365-76 pubmed publisher
Liang G, Liu G, Kitamura K, Wang Z, Chowdhury S, Monjurul A, et al. TGF-β suppression of HBV RNA through AID-dependent recruitment of an RNA exosome complex. PLoS Pathog. 2015;11:e1004780 pubmed publisher
Wang H, Geng J, Wen X, Bi E, Kossenkov A, Wolf A, et al. The transcription factor Foxp1 is a critical negative regulator of the differentiation of follicular helper T cells. Nat Immunol. 2014;15:667-75 pubmed publisher
Shimamoto R, Amano N, Ichisaka T, Watanabe A, Yamanaka S, Okita K. Generation and characterization of induced pluripotent stem cells from Aid-deficient mice. PLoS ONE. 2014;9:e94735 pubmed publisher
Basso K, Schneider C, Shen Q, Holmes A, Setty M, Leslie C, et al. BCL6 positively regulates AID and germinal center gene expression via repression of miR-155. J Exp Med. 2012;209:2455-65 pubmed publisher
Tsuji M, Komatsu N, Kawamoto S, Suzuki K, Kanagawa O, Honjo T, et al. Preferential generation of follicular B helper T cells from Foxp3+ T cells in gut Peyer's patches. Science. 2009;323:1488-92 pubmed publisher
Tsuji M, Suzuki K, Kitamura H, Maruya M, Kinoshita K, Ivanov I, et al. Requirement for lymphoid tissue-inducer cells in isolated follicle formation and T cell-independent immunoglobulin A generation in the gut. Immunity. 2008;29:261-71 pubmed publisher
Kolar G, Mehta D, Pelayo R, Capra J. A novel human B cell subpopulation representing the initial germinal center population to express AID. Blood. 2007;109:2545-52 pubmed
Honjo T, Muramatsu M, Fagarasan S. AID: how does it aid antibody diversity?. Immunity. 2004;20:659-68 pubmed
Okazaki I, Hiai H, Kakazu N, Yamada S, Muramatsu M, Kinoshita K, et al. Constitutive expression of AID leads to tumorigenesis. J Exp Med. 2003;197:1173-81 pubmed
Muramatsu M, Sankaranand V, Anant S, Sugai M, Kinoshita K, Davidson N, et al. Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells. J Biol Chem. 1999;274:18470-6 pubmed
product information
Product Type :
Antibody
Product Name :
AID Monoclonal Antibody (mAID-2), eBioscience
Catalog # :
14-5959-82
Quantity :
100 µg
Price :
US 450.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Human, Mouse
Applications :
Immunohistochemistry (Frozen): 20 µg/mL, Immunohistochemistry (Paraffin): 20 µg/mL, Western Blot: 20 µg/mL
Species :
Human, Mouse
Clone :
mAID-2
Isotype :
IgG2a, kappa
Storage :
4° C
Description :
This gene encodes a RNA-editing deaminase that is a member of the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes. Defects in this gene are the cause of autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2).
Format :
Liquid
Applications w/Dilutions :
Immunohistochemistry (Frozen): 20 µg/mL, Immunohistochemistry (Paraffin): 20 µg/mL, Western Blot: 20 µg/mL
Aliases :
activation induced deaminase; activation-induced cytidine deaminase; Aicda; AID; Arp2; CDA2; Cytidine aminohydrolase; epididymis secretory protein Li 284; HEL-S-284; HIGM2; integrated into Burkitt's lymphoma cell line Ramos; single-stranded DNA cytosine deaminase
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA