CD254 (RANK Ligand) Monoclonal Antibody (IK22/5), eBioscience | Invitrogen 14-5952-82 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD254 (RANK Ligand) Monoclonal Antibody (IK22/5), eBioscience

catalog :

14-5952-82

quantity :

100 µg

price :

US 323.00

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

IK22/5

reactivity :

mouse

application :

immunohistochemistry, flow cytometry, immunohistochemistry - frozen section, immunohistochemistry knockout validation

more info or order :

Invitrogen product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry knockout validation; mouse; fig 1 immunohistochemistry - frozen section; mouse; fig 1	Taylor R, Patel S, Lin E, Butler B, Lake J, Newberry R, et al. Lymphotoxin-independent expression of TNF-related activation-induced cytokine by stromal cells in cryptopatches, isolated lymphoid follicles, and Peyer's patches. J Immunol. 2007;178:5659-67 pubmed
immunohistochemistry - frozen section; mouse; 1:100; fig s2a	Kimura S, Nakamura Y, Kobayashi N, Shiroguchi K, Kawakami E, Mutoh M, et al. Osteoprotegerin-dependent M cell self-regulation balances gut infection and immunity. Nat Commun. 2020;11:234 pubmed publisher
flow cytometry; mouse; loading ...; fig 6c	Jones R, Cosway E, Willis C, White A, Jenkinson W, Fehling H, et al. Dynamic changes in intrathymic ILC populations during murine neonatal development. Eur J Immunol. 2018;48:1481-1491 pubmed publisher
immunohistochemistry; mouse; 1:100; fig 4	Parnell E, Walch E, Lo D. Inducible Colonic M Cells Are Dependent on TNFR2 but Not Lt?r, Identifying Distinct Signalling Requirements for Constitutive Versus Inducible M Cells. J Crohns Colitis. 2017;11:751-760 pubmed publisher
immunohistochemistry - frozen section; mouse; fig 3a	Kobayashi A, Donaldson D, Erridge C, Kanaya T, Williams I, Ohno H, et al. The functional maturation of M cells is dramatically reduced in the Peyer's patches of aged mice. Mucosal Immunol. 2013;6:1027-37 pubmed publisher
flow cytometry; mouse; fig 2	Nakae S, Iwakura Y, Suto H, Galli S. Phenotypic differences between Th1 and Th17 cells and negative regulation of Th1 cell differentiation by IL-17. J Leukoc Biol. 2007;81:1258-68 pubmed
	Li J, Ayoub A, Xiu Y, Yin X, Sanders J, Mesfin A, et al. TGFβ-induced degradation of TRAF3 in mesenchymal progenitor cells causes age-related osteoporosis. Nat Commun. 2019;10:2795 pubmed publisher
	Thiele S, Ziegler N, Tsourdi E, De Bosscher K, Tuckermann J, Hofbauer L, et al. Selective glucocorticoid receptor modulation maintains bone mineral density in mice. J Bone Miner Res. 2012;27:2242-50 pubmed publisher
	Douni E, Rinotas V, Makrinou E, Zwerina J, Penninger J, Eliopoulos E, et al. A RANKL G278R mutation causing osteopetrosis identifies a functional amino acid essential for trimer assembly in RANKL and TNF. Hum Mol Genet. 2012;21:784-98 pubmed publisher
	Vondenhoff M, Greuter M, Goverse G, Elewaut D, Dewint P, Ware C, et al. LTbetaR signaling induces cytokine expression and up-regulates lymphangiogenic factors in lymph node anlagen. J Immunol. 2009;182:5439-45 pubmed publisher
	Kamijo S, Nakajima A, Ikeda K, Aoki K, Ohya K, Akiba H, et al. Amelioration of bone loss in collagen-induced arthritis by neutralizing anti-RANKL monoclonal antibody. Biochem Biophys Res Commun. 2006;347:124-32 pubmed
	Cremer I, Dieu Nosjean M, Maréchal S, Dezutter Dambuyant C, Goddard S, Adams D, et al. Long-lived immature dendritic cells mediated by TRANCE-RANK interaction. Blood. 2002;100:3646-55 pubmed
	Wong B, Josien R, Choi Y. TRANCE is a TNF family member that regulates dendritic cell and osteoclast function. J Leukoc Biol. 1999;65:715-24 pubmed

product information

Product Type :

Antibody

Product Name :

CD254 (RANK Ligand) Monoclonal Antibody (IK22/5), eBioscience

Catalog # :

14-5952-82

Quantity :

100 µg

Price :

US 323.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Rat

Reactivity :

Mouse

Applications :

Flow Cytometry: 0.5 µg/test, Functional Assay: Assay-Dependent

Species :

Mouse

Clone :

IK22/5

Isotype :

IgG2a, kappa

Storage :

4° C

Description :

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.5 µg/test, Functional Assay: Assay-Dependent

Aliases :

CD254; H-RANK-L; hRANKL2; Ly109l; M-Rank-L; ODF; OPG; OPG ligand; OPGL; OPTB2; Osteoclast differentiation factor; osteoprotegerin ligand; Rankl; rank-l; receptor activator of NF-kappaB ligand; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand; RP11-86N24.2; sOdf; srankl; TNF-related activation-induced cytokine; Tnfsf11; TNLG6B; Trance; tumor necrosis factor (ligand) superfamily member 11; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand 6B; tumor necrosis factor ligand superfamily member 11; Tumor necrosis factor ligand superfamily member 11, membrane form; Tumor necrosis factor ligand superfamily member 11, soluble form; tumor necrosis factor superfamily member 11; tumor necrosis factor-related activation-induced cytokine

more info or order :

Invitrogen product webpage