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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
GL7 Monoclonal Antibody (GL-7 (GL7)), eBioscience
catalog :
14-5902-81
quantity :
50 µg
price :
US 78.75
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
GL-7 (GL7)
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 66
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:200; loading ...; fig s7c
Lacy M, Burger C, Shami A, Ahmadsei M, Winkels H, Nitz K, et al. Cell-specific and divergent roles of the CD40L-CD40 axis in atherosclerotic vascular disease. Nat Commun. 2021;12:3754 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3b
Lin X, Twelkmeyer T, Zhu D, Zhang L, Zhao Y, Zhang C, et al. Homeostatic regulation of T follicular helper and antibody response to particle antigens by IL-1Ra of medullary sinus macrophage origin. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4f
Svensson M, Zoccheddu M, Yang S, Nygaard G, Secchi C, Doody K, et al. Synoviocyte-targeted therapy synergizes with TNF inhibition in arthritis reversal. Sci Adv. 2020;6:eaba4353 pubmed publisher
  • flow cytometry; mouse; 1:120; loading ...; fig 1s1b
Koike T, Harada K, Horiuchi S, Kitamura D. The quantity of CD40 signaling determines the differentiation of B cells into functionally distinct memory cell subsets. elife. 2019;8: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1e
Li F, Zeng Z, Xing S, Gullicksrud J, Shan Q, Choi J, et al. Ezh2 programs TFH differentiation by integrating phosphorylation-dependent activation of Bcl6 and polycomb-dependent repression of p19Arf. Nat Commun. 2018;9:5452 pubmed publisher
  • flow cytometry; mouse; 1:500; loading ...; fig s3a
Cho S, Lee H, Yu I, Choi Y, Huang H, Hashemifar S, et al. Differential cell-intrinsic regulations of germinal center B and T cells by miR-146a and miR-146b. Nat Commun. 2018;9:2757 pubmed publisher
  • flow cytometry; mouse; fig 3c
Yang K, Liang Y, Sun Z, Xue D, Xu H, Zhu M, et al. T Cell-Derived Lymphotoxin Is Essential for the Anti-Herpes Simplex Virus 1 Humoral Immune Response. J Virol. 2018;92: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 7c
Georgiev H, Ravens I, Papadogianni G, Halle S, Malissen B, Loots G, et al. Shared and Unique Features Distinguishing Follicular T Helper and Regulatory Cells of Peripheral Lymph Node and Peyer's Patches. Front Immunol. 2018;9:714 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 8d
Chen Y, Chaudhary N, Yang N, Granato A, Turner J, Howard S, et al. Microbial symbionts regulate the primary Ig repertoire. J Exp Med. 2018;215:1397-1415 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s8
Gaddis D, Padgett L, Wu R, McSkimming C, Romines V, Taylor A, et al. Apolipoprotein AI prevents regulatory to follicular helper T cell switching during atherosclerosis. Nat Commun. 2018;9:1095 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5b
Nadafi R, Koning J, Veninga H, Stachtea X, Konijn T, Zwiers A, et al. Dendritic Cell Migration to Skin-Draining Lymph Nodes Is Controlled by Dermatan Sulfate and Determines Adaptive Immunity Magnitude. Front Immunol. 2018;9:206 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2a
Ferdinand J, Richard A, Meylan F, Al Shamkhani A, Siegel R. Cleavage of TL1A Differentially Regulates Its Effects on Innate and Adaptive Immune Cells. J Immunol. 2018;200:1360-1369 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; loading ...; fig s2
Sitrin J, Suto E, Wuster A, Eastham Anderson J, Kim J, Austin C, et al. The Ox40/Ox40 Ligand Pathway Promotes Pathogenic Th Cell Responses, Plasmablast Accumulation, and Lupus Nephritis in NZB/W F1 Mice. J Immunol. 2017;199:1238-1249 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Inoue T, Shinnakasu R, Ise W, Kawai C, Egawa T, Kurosaki T. The transcription factor Foxo1 controls germinal center B cell proliferation in response to T cell help. J Exp Med. 2017;214:1181-1198 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a
Rubtsova K, Rubtsov A, Thurman J, Mennona J, Kappler J, Marrack P. B cells expressing the transcription factor T-bet drive lupus-like autoimmunity. J Clin Invest. 2017;127:1392-1404 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6c
van Nieuwenhuijze A, Dooley J, Humblet Baron S, Sreenivasan J, Koenders M, Schlenner S, et al. Defective germinal center B-cell response and reduced arthritic pathology in microRNA-29a-deficient mice. Cell Mol Life Sci. 2017;74:2095-2106 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 7
Chen S, Cai C, Li Z, Liu G, Wang Y, Blonska M, et al. Dissection of SAP-dependent and SAP-independent SLAM family signaling in NKT cell development and humoral immunity. J Exp Med. 2017;214:475-489 pubmed publisher
  • flow cytometry; mouse; fig 9c
Massaad M, Zhou J, Tsuchimoto D, Chou J, Jabara H, Janssen E, et al. Deficiency of base excision repair enzyme NEIL3 drives increased predisposition to autoimmunity. J Clin Invest. 2016;126:4219-4236 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Chen S, Miyazaki M, Chandra V, Fisch K, Chang A, Murre C. Id3 Orchestrates Germinal Center B Cell Development. Mol Cell Biol. 2016;36:2543-52 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6b
Jackson S, Jacobs H, Arkatkar T, Dam E, Scharping N, Kolhatkar N, et al. B cell IFN-γ receptor signaling promotes autoimmune germinal centers via cell-intrinsic induction of BCL-6. J Exp Med. 2016;213:733-50 pubmed publisher
  • flow cytometry; mouse
Choi Y, Gullicksrud J, Xing S, Zeng Z, Shan Q, Li F, et al. LEF-1 and TCF-1 orchestrate T(FH) differentiation by regulating differentiation circuits upstream of the transcriptional repressor Bcl6. Nat Immunol. 2015;16:980-90 pubmed publisher
  • flow cytometry; mouse
Dahlgren M, Gustafsson Hedberg T, Livingston M, Cucak H, Alsén S, Yrlid U, et al. T follicular helper, but not Th1, cell differentiation in the absence of conventional dendritic cells. J Immunol. 2015;194:5187-99 pubmed publisher
  • flow cytometry; mouse; fig 3
Shrestha S, Yang K, Guy C, Vogel P, Neale G, Chi H. Treg cells require the phosphatase PTEN to restrain TH1 and TFH cell responses. Nat Immunol. 2015;16:178-87 pubmed publisher
  • flow cytometry; mouse
Denton A, Roberts E, Linterman M, Fearon D. Fibroblastic reticular cells of the lymph node are required for retention of resting but not activated CD8+ T cells. Proc Natl Acad Sci U S A. 2014;111:12139-44 pubmed publisher
  • western blot; human
Wang J, New J, Xie S, Yang P, Wu Q, Li J, et al. Extension of the germinal center stage of B cell development promotes autoantibodies in BXD2 mice. Arthritis Rheum. 2013;65:2703-12 pubmed publisher
  • flow cytometry; mouse
Billich A, Baumruker T, Beerli C, Bigaud M, Bruns C, Calzascia T, et al. Partial deficiency of sphingosine-1-phosphate lyase confers protection in experimental autoimmune encephalomyelitis. PLoS ONE. 2013;8:e59630 pubmed publisher
  • flow cytometry; mouse
Purtha W, Tedder T, Johnson S, Bhattacharya D, Diamond M. Memory B cells, but not long-lived plasma cells, possess antigen specificities for viral escape mutants. J Exp Med. 2011;208:2599-606 pubmed publisher
Rosser E, Piper C, Matei D, Blair P, Rendeiro A, Orford M, et al. Microbiota-Derived Metabolites Suppress Arthritis by Amplifying Aryl-Hydrocarbon Receptor Activation in Regulatory B Cells. Cell Metab. 2020;31:837-851.e10 pubmed publisher
Zhang C, Wang C, Jiang M, Gu C, Xiao J, Chen X, et al. Act1 is a negative regulator in T and B cells via direct inhibition of STAT3. Nat Commun. 2018;9:2745 pubmed publisher
Raso F, Sagadiev S, Du S, Gage E, Arkatkar T, Metzler G, et al. αv Integrins regulate germinal center B cell responses through noncanonical autophagy. J Clin Invest. 2018;128:4163-4178 pubmed publisher
Mande P, Zirak B, Ko W, Taravati K, Bride K, Brodeur T, et al. Fas ligand promotes an inducible TLR-dependent model of cutaneous lupus-like inflammation. J Clin Invest. 2018;128:2966-2978 pubmed publisher
Barwick B, Scharer C, Martinez R, Price M, Wein A, Haines R, et al. B cell activation and plasma cell differentiation are inhibited by de novo DNA methylation. Nat Commun. 2018;9:1900 pubmed publisher
Liu J, Huang X, Hao S, Wang Y, Liu M, Xu J, et al. Peli1 negatively regulates noncanonical NF-κB signaling to restrain systemic lupus erythematosus. Nat Commun. 2018;9:1136 pubmed publisher
Wang X, Zhang Y, Wang Z, Liu X, Zhu G, Han G, et al. Anti?IL?39 (IL?23p19/Ebi3) polyclonal antibodies ameliorate autoimmune symptoms in lupus?like mice. Mol Med Rep. 2017;: pubmed publisher
Deng R, Hurtz C, Song Q, Yue C, Xiao G, Yu H, et al. Extrafollicular CD4+ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease. Nat Commun. 2017;8:978 pubmed publisher
Sun J, Holmgren J, Larena M, Terrinoni M, Fang Y, Bresnick A, et al. Deficiency in Calcium-Binding Protein S100A4 Impairs the Adjuvant Action of Cholera Toxin. Front Immunol. 2017;8:1119 pubmed publisher
Miletić A, Lenartic M, Popovic B, Brizic I, Trsan T, Miklic K, et al. NCR1-deficiency diminishes the generation of protective murine cytomegalovirus antibodies by limiting follicular helper T-cell maturation. Eur J Immunol. 2017;47:1443-1456 pubmed publisher
Bemark M, Hazanov H, Strömberg A, Komban R, Holmqvist J, Köster S, et al. Limited clonal relatedness between gut IgA plasma cells and memory B cells after oral immunization. Nat Commun. 2016;7:12698 pubmed publisher
de Winde C, Veenbergen S, Young K, Xu Monette Z, Wang X, Xia Y, et al. Tetraspanin CD37 protects against the development of B cell lymphoma. J Clin Invest. 2016;126:653-66 pubmed publisher
Matino D, Gargaro M, Santagostino E, Di Minno M, Castaman G, Morfini M, et al. IDO1 suppresses inhibitor development in hemophilia A treated with factor VIII. J Clin Invest. 2015;125:3766-81 pubmed publisher
Kuzin I, Bouta E, Schwarz E, Bottaro A. TNF signals are dispensable for the generation of CD23+ CD21/35-high CD1d-high B cells in inflamed lymph nodes. Cell Immunol. 2015;296:133-7 pubmed publisher
Flynn R, Allen J, Luznik L, MacDonald K, Paz K, Alexander K, et al. Targeting Syk-activated B cells in murine and human chronic graft-versus-host disease. Blood. 2015;125:4085-94 pubmed publisher
Pascutti M, Geerman S, Slot E, van Gisbergen K, Boon L, Arens R, et al. Enhanced CD8 T cell responses through GITR-mediated costimulation resolve chronic viral infection. PLoS Pathog. 2015;11:e1004675 pubmed publisher
Funakoshi S, Shimizu T, Numata O, Ato M, Melchers F, Ohnishi K. BILL-cadherin/cadherin-17 contributes to the survival of memory B cells. PLoS ONE. 2015;10:e0117566 pubmed publisher
Kim J, Li W, Choi Y, Lewin S, Verbeke C, Dranoff G, et al. Injectable, spontaneously assembling, inorganic scaffolds modulate immune cells in vivo and increase vaccine efficacy. Nat Biotechnol. 2015;33:64-72 pubmed publisher
Senejani A, Liu Y, Kidane D, Maher S, Zeiss C, Park H, et al. Mutation of POLB causes lupus in mice. Cell Rep. 2014;6:1-8 pubmed publisher
Hua Z, Gross A, Lamagna C, Ramos Hernández N, Scapini P, Ji M, et al. Requirement for MyD88 signaling in B cells and dendritic cells for germinal center anti-nuclear antibody production in Lyn-deficient mice. J Immunol. 2014;192:875-85 pubmed publisher
Panda S, Zhang J, Tan N, Ho B, Ding J. Natural IgG antibodies provide innate protection against ficolin-opsonized bacteria. EMBO J. 2013;32:2905-19 pubmed publisher
Block K, Huang H. The cellular source and target of IL-21 in K/BxN autoimmune arthritis. J Immunol. 2013;191:2948-55 pubmed publisher
Detre C, Yigit B, Keszei M, Castro W, Magelky E, Terhorst C. SAP modulates B cell functions in a genetic background-dependent manner. Immunol Lett. 2013;153:15-21 pubmed publisher
Hirota K, Turner J, Villa M, Duarte J, Demengeot J, Steinmetz O, et al. Plasticity of Th17 cells in Peyer's patches is responsible for the induction of T cell-dependent IgA responses. Nat Immunol. 2013;14:372-9 pubmed publisher
Ramon S, Bancos S, Thatcher T, Murant T, Moshkani S, Sahler J, et al. Peroxisome proliferator-activated receptor ? B cell-specific-deficient mice have an impaired antibody response. J Immunol. 2012;189:4740-7 pubmed publisher
Freire T, Zhang X, Deriaud E, Ganneau C, Vichier Guerre S, Azria E, et al. Glycosidic Tn-based vaccines targeting dermal dendritic cells favor germinal center B-cell development and potent antibody response in the absence of adjuvant. Blood. 2010;116:3526-36 pubmed publisher
Sin S, Fakhari F, Dittmer D. The viral latency-associated nuclear antigen augments the B-cell response to antigen in vivo. J Virol. 2010;84:10653-60 pubmed publisher
Liu Y, Zhang W. Identification of a new transmembrane adaptor protein that constitutively binds Grb2 in B cells. J Leukoc Biol. 2008;84:842-51 pubmed publisher
DiLillo D, Hamaguchi Y, Ueda Y, Yang K, Uchida J, Haas K, et al. Maintenance of long-lived plasma cells and serological memory despite mature and memory B cell depletion during CD20 immunotherapy in mice. J Immunol. 2008;180:361-71 pubmed
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Kimura N, Ohmori K, Miyazaki K, Izawa M, Matsuzaki Y, Yasuda Y, et al. Human B-lymphocytes express alpha2-6-sialylated 6-sulfo-N-acetyllactosamine serving as a preferred ligand for CD22/Siglec-2. J Biol Chem. 2007;282:32200-7 pubmed
Meier D, Bornmann C, Chappaz S, Schmutz S, Otten L, Ceredig R, et al. Ectopic lymphoid-organ development occurs through interleukin 7-mediated enhanced survival of lymphoid-tissue-inducer cells. Immunity. 2007;26:643-54 pubmed
Naito Y, Takematsu H, Koyama S, Miyake S, Yamamoto H, Fujinawa R, et al. Germinal center marker GL7 probes activation-dependent repression of N-glycolylneuraminic acid, a sialic acid species involved in the negative modulation of B-cell activation. Mol Cell Biol. 2007;27:3008-22 pubmed
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product information
Product Type :
Antibody
Product Name :
GL7 Monoclonal Antibody (GL-7 (GL7)), eBioscience
Catalog # :
14-5902-81
Quantity :
50 µg
Price :
US 78.75
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Human, Mouse
Applications :
Flow Cytometry: 0.5 µg/test, Immunohistochemistry (Frozen): Assay-Dependent, Immunoprecipitation: Assay-Dependent
Species :
Human, Mouse
Clone :
GL-7 (GL7)
Isotype :
IgM
Storage :
4° C
Description :
GL7 is a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.5 µg/test, Immunohistochemistry (Frozen): Assay-Dependent, Immunoprecipitation: Assay-Dependent
Aliases :
GL7 antigen; Ly77; Ly-77
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA