product summary
Loading...
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
ISG15 Monoclonal Antibody (3C2), eBioscience™
catalog :
14-5857-80
quantity :
25µg
price :
US 148
clonality :
monoclonal
host :
hamsters
conjugate :
nonconjugated
clone name :
3C2
reactivity :
mouse, rat
application :
western blot
more info or order :
Invitrogen product webpage
citations: 2
Published Application/Species/Sample/DilutionReference
  • western blot; rat; fig 3
Gilda J, Ghosh R, Cheah J, West T, Bodine S, Gomes A. Western Blotting Inaccuracies with Unverified Antibodies: Need for a Western Blotting Minimal Reporting Standard (WBMRS). PLoS ONE. 2015;10:e0135392 pubmed publisher
Frias Staheli N, Giannakopoulos N, Kikkert M, Taylor S, Bridgen A, Paragas J, et al. Ovarian tumor domain-containing viral proteases evade ubiquitin- and ISG15-dependent innate immune responses. Cell Host Microbe. 2007;2:404-16 pubmed
product information
Product Type :
Antibody
Product Name :
ISG15 Monoclonal Antibody (3C2), eBioscience™
Catalog # :
14-5857-80
Quantity :
25µg
Price :
US 148
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Armenian Hamster
Reactivity :
Mouse
Applications :
Western Blot: 2 µg/mL
Species :
Mouse
Clone :
3C2
Isotype :
IgG
Storage :
4° C
Description :
Ubiquitin-like protein ISG15 plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. It is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and an augmentation of non-MCH (major histocompatibility complex)-restricted cytotoxicity. Synthesis is stimulated by IFN-alpha or IFN-beta or IFN-omega , but not IFN-gamma . ISG15 expression is also induced by overexpression of interferon regulatory factors that participate in transcriptional regulation of IFN genes, and by influenza B virus. ISG15 is secreted by cell lines of monocyte, T-lymphocyte, B-lymphocyte, human fibroblasts, and epithelial origins. Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade and suggest that it may be responsible for amplifying and directing some of the immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 has has also been shown to function intracellularly as a ubiquitin homolog. Mutations affecting the gene can result in Immunodeficiency 38, with basal ganglia calcification (IMD38).
Format :
Liquid
Applications w/Dilutions :
Western Blot: 2 µg/mL
Aliases :
G1p2; hUCRP; IFI15; IGI15; IMD38; interferon, alpha-inducible protein; interferon, alpha-inducible protein (clone IFI-15K); Interferon-induced 15 kDa protein; interferon-induced 15-KDa protein; Interferon-induced 17 kDa protein; interferon-induced 17-kDa/15-kDa protein; interferon-stimulated protein (15 kDa); interferon-stimulated protein 15; interferon-stimulated protein, 15 kDa; IP17; Irfp; Isg15; ISG15 ubiquitin-like modifier; Ubiquitin cross-reactive protein; Ubiquitin-like protein ISG15; Ucrp
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA