Nestin Monoclonal Antibody (Rat-401 (Rat401 (4D4))), eBioscience™ | Invitrogen 14-5843-80 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Nestin Monoclonal Antibody (Rat-401 (Rat401 (4D4))), eBioscience™

catalog :

14-5843-80

quantity :

25µg

price :

US 108

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Rat-401 (Rat401 (4D4))

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; loading ...; fig 1d	Obora K, Onodera Y, Takehara T, Frampton J, Hasei J, Ozaki T, et al. Inflammation-induced miRNA-155 inhibits self-renewal of neural stem cells via suppression of CCAAT/enhancer binding protein β (C/EBPβ) expression. Sci Rep. 2017;7:43604 pubmed publisher
	Clarke D, Pearse Y, Kan S, Le S, Sanghez V, Cooper J, et al. Genetically Corrected iPSC-Derived Neural Stem Cell Grafts Deliver Enzyme Replacement to Affect CNS Disease in Sanfilippo B Mice. Mol Ther Methods Clin Dev. 2018;10:113-127 pubmed publisher
	Connolly N, Stokum J, Schneider C, Ozawa T, Xu S, Galisteo R, et al. Genetically engineered rat gliomas: PDGF-driven tumor initiation and progression in tv-a transgenic rats recreate key features of human brain cancer. PLoS ONE. 2017;12:e0174557 pubmed publisher
	Francipane M, Lagasse E. Maturation of embryonic tissues in a lymph node: a new approach for bioengineering complex organs. Organogenesis. 2014;10:323-31 pubmed publisher
	Lindsley R, Gill J, Kyba M, Murphy T, Murphy K. Canonical Wnt signaling is required for development of embryonic stem cell-derived mesoderm. Development. 2006;133:3787-96 pubmed
	Kachinsky A, Dominov J, Miller J. Intermediate filaments in cardiac myogenesis: nestin in the developing mouse heart. J Histochem Cytochem. 1995;43:843-7 pubmed
	Hockfield S, McKay R. Identification of major cell classes in the developing mammalian nervous system. J Neurosci. 1985;5:3310-28 pubmed

product information

Product Type :

Antibody

Product Name :

Nestin Monoclonal Antibody (Rat-401 (Rat401 (4D4))), eBioscience™

Catalog # :

14-5843-80

Quantity :

25µg

Price :

US 108

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Mouse, Rat

Applications :

Immunocytochemistry: Assay-Dependent, Immunofluorescence: Assay-Dependent, Immunohistochemistry (Frozen): 10 µg/mL, Immunohistochemistry (Paraffin): 10 µg/mL, Western Blot: Assay-Dependent

Species :

Mouse, Rat

Clone :

Rat-401 (Rat401 (4D4))

Isotype :

IgG1

Storage :

4° C

Description :

Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: Assay-Dependent, Immunofluorescence: Assay-Dependent, Immunohistochemistry (Frozen): 10 µg/mL, Immunohistochemistry (Paraffin): 10 µg/mL, Western Blot: Assay-Dependent

Aliases :

AA166324; C78523; ESTM46; FLJ21841; intermediate filament protein; Nbla00170; NES; Nestin

more info or order :

Invitrogen product webpage