OCT3/4 Monoclonal Antibody (EM92), eBioscience™ | Invitrogen 14-5841-80 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

OCT3/4 Monoclonal Antibody (EM92), eBioscience™

catalog :

14-5841-80

quantity :

25µg

price :

US 148

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

EM92

reactivity :

human, mouse

application :

western blot, immunohistochemistry, flow cytometry

more info or order :

Invitrogen product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig s3a	Byrne S, Ortiz L, Mali P, Aach J, Church G. Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells. Nucleic Acids Res. 2015;43:e21 pubmed publisher
	Bohr S, Patel S, Vasko R, Shen K, Huang G, Yarmush M, et al. Highly upregulated Lhx2 in the Foxn1-/- nude mouse phenotype reflects a dysregulated and expanded epidermal stem cell niche. PLoS ONE. 2013;8:e64223 pubmed publisher
	Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 2007;131:861-72 pubmed
	Pikarsky E, Sharir H, Ben Shushan E, Bergman Y. Retinoic acid represses Oct-3/4 gene expression through several retinoic acid-responsive elements located in the promoter-enhancer region. Mol Cell Biol. 1994;14:1026-38 pubmed
	Okamoto K, Okazawa H, Okuda A, Sakai M, Muramatsu M, Hamada H. A novel octamer binding transcription factor is differentially expressed in mouse embryonic cells. Cell. 1990;60:461-72 pubmed

product information

Product Type :

Antibody

Product Name :

OCT3/4 Monoclonal Antibody (EM92), eBioscience™

Catalog # :

14-5841-80

Quantity :

25µg

Price :

US 148

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Rat

Reactivity :

Human, Mouse

Applications :

Immunohistochemistry: Assay-Dependent, Western Blot: 2 µg/mL

Species :

Human, Mouse

Clone :

EM92

Isotype :

IgG2a, kappa

Storage :

4° C

Description :

POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry: Assay-Dependent, Western Blot: 2 µg/mL

Aliases :

3-Oct; 4-Oct; DADB-104B20.2; gp-9; HGNC:9221; MGC22487; NF-A3; OCT3; Oct-3; Oct3/4; Oct-3/4; OCT4; Oct-4; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; Otf3; Otf-3; Otf3g; Otf3-rs7; Otf4; Otf-4; OTTHUMP00000221150; OTTHUMP00000221151; POU class 5 homeobox 1; POU class 5 homeobox 1 transcript variant OCT4B1; POU class 5 homeobox 1 transcript variant OCT4B2; POU class 5 homeobox 1 transcript variant OCT4B3; POU class 5 homeobox 1 transcript variant OCT4B4; POU class 5 homeobox 1 transcript variant OCT4B5; POU class 5 homeobox 1 transcript variant OCT4B6; POU domain class 5 transcription factor 1; POU domain transcription factor OCT4; POU domain, class 5, transcription factor 1; pou2; pou-2; Pou5f1; POU-type homeodomain-containing DNA-binding protein

more info or order :

Invitrogen product webpage