product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD326 (EpCAM) Monoclonal Antibody (G8.8), eBioscience
catalog :
14-5791-81
quantity :
50 µg
price :
US 79.25
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
G8.8
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, blocking or activating experiments
more info or order :
citations: 104
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...
McElrath C, Espinosa V, Lin J, Peng J, Sridhar R, Dutta O, et al. Critical role of interferons in gastrointestinal injury repair. Nat Commun. 2021;12:2624 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 4g
Harbour S, DiToro D, Witte S, Zindl C, Gao M, Schoeb T, et al. TH17 cells require ongoing classic IL-6 receptor signaling to retain transcriptional and functional identity. Sci Immunol. 2020;5: pubmed publisher
  • immunohistochemistry; mouse; 1:50; loading ...; fig 4a
Guiu J, Hannezo E, Yui S, Demharter S, Ulyanchenko S, Maimets M, et al. Tracing the origin of adult intestinal stem cells. Nature. 2019;570:107-111 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 1a
Ke X, Do D, Li C, Zhao Y, Kollarik M, Fu Q, et al. Ras homolog family member A/Rho-associated protein kinase 1 signaling modulates lineage commitment of mesenchymal stem cells in asthmatic patients through lymphoid enhancer-binding factor 1. J Allergy Clin Immunol. 2019;143:1560-1574.e6 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 1f
Ernszt D, Banfai K, Kellermayer Z, Pap A, Lord J, Pongracz J, et al. PPARgamma Deficiency Counteracts Thymic Senescence. Front Immunol. 2017;8:1515 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1f
Carrieri C, Comazzetto S, Grover A, Morgan M, Buness A, Nerlov C, et al. A transit-amplifying population underpins the efficient regenerative capacity of the testis. J Exp Med. 2017;214:1631-1641 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3c
Cuccarese M, Dubach J, Pfirschke C, Engblom C, Garris C, Miller M, et al. Heterogeneity of macrophage infiltration and therapeutic response in lung carcinoma revealed by 3D organ imaging. Nat Commun. 2017;8:14293 pubmed publisher
  • western blot; mouse; loading ...; fig s3a
Kuan I, Liang K, Wang Y, Kuo T, Meir Y, Wu S, et al. EpEX/EpCAM and Oct4 or Klf4 alone are sufficient to generate induced pluripotent stem cells through STAT3 and HIF2α. Sci Rep. 2017;7:41852 pubmed publisher
  • immunohistochemistry; mouse; 1:200; loading ...; fig 5m
Yang Z, Peng Y, Gopalan A, Gao D, Chen Y, Joyner A. Stromal hedgehog signaling maintains smooth muscle and hampers micro-invasive prostate cancer. Dis Model Mech. 2017;10:39-52 pubmed publisher
  • flow cytometry; mouse; loading ...; fig S1A
Marichal T, Gaudenzio N, El Abbas S, Sibilano R, Zurek O, Starkl P, et al. Guanine nucleotide exchange factor RABGEF1 regulates keratinocyte-intrinsic signaling to maintain skin homeostasis. J Clin Invest. 2016;126:4497-4515 pubmed publisher
  • flow cytometry; mouse; 1:1000; fig 6c
Zhang Y, Velez Delgado A, Mathew E, Li D, Mendez F, Flannagan K, et al. Myeloid cells are required for PD-1/PD-L1 checkpoint activation and the establishment of an immunosuppressive environment in pancreatic cancer. Gut. 2017;66:124-136 pubmed publisher
  • flow cytometry; mouse; fig s4
Beyaz S, Mana M, Roper J, Kedrin D, Saadatpour A, Hong S, et al. High-fat diet enhances stemness and tumorigenicity of intestinal progenitors. Nature. 2016;531:53-8 pubmed publisher
  • flow cytometry; mouse; 1:300; fig e3
von Moltke J, Ji M, Liang H, Locksley R. Tuft-cell-derived IL-25 regulates an intestinal ILC2-epithelial response circuit. Nature. 2016;529:221-5 pubmed publisher
  • flow cytometry; mouse; fig 3e
Ziętara N, Łyszkiewicz M, Puchałka J, Witzlau K, Reinhardt A, Förster R, et al. Multicongenic fate mapping quantification of dynamics of thymus colonization. J Exp Med. 2015;212:1589-601 pubmed publisher
  • flow cytometry; mouse; 1:100; fig 4
Zoch A, Mayerl S, Schulz A, Greither T, Frappart L, Rübsam J, et al. Merlin Isoforms 1 and 2 Both Act as Tumour Suppressors and Are Required for Optimal Sperm Maturation. PLoS ONE. 2015;10:e0129151 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1
Woods S, Waite A, O Dea K, Halford P, Takata M, Wilson M. Kinetic profiling of in vivo lung cellular inflammatory responses to mechanical ventilation. Am J Physiol Lung Cell Mol Physiol. 2015;308:L912-21 pubmed publisher
  • flow cytometry; mouse; fig 3
Velardi E, Tsai J, Holland A, Wertheimer T, Yu V, Zakrzewski J, et al. Sex steroid blockade enhances thymopoiesis by modulating Notch signaling. J Exp Med. 2014;211:2341-9 pubmed publisher
  • flow cytometry; mouse; fig 6
Xia H, Ren X, Bolte C, Ustiyan V, Zhang Y, Shah T, et al. Foxm1 regulates resolution of hyperoxic lung injury in newborns. Am J Respir Cell Mol Biol. 2015;52:611-21 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:800
Zhou Q, Ho A, Schlitzer A, Tang Y, Wong K, Wong F, et al. GM-CSF-licensed CD11b+ lung dendritic cells orchestrate Th2 immunity to Blomia tropicalis. J Immunol. 2014;193:496-509 pubmed publisher
  • blocking or activating experiments; human
Treutlein B, Brownfield D, Wu A, Neff N, Mantalas G, Espinoza F, et al. Reconstructing lineage hierarchies of the distal lung epithelium using single-cell RNA-seq. Nature. 2014;509:371-5 pubmed publisher
  • flow cytometry; mouse; 10 ug/ml; fig 3
Hittinger M, Czyz Z, Huesemann Y, Maneck M, Botteron C, Kaeufl S, et al. Molecular profiling of single Sca-1+/CD34+,- cells--the putative murine lung stem cells. PLoS ONE. 2013;8:e83917 pubmed publisher
  • flow cytometry; mouse
Venanzi E, Gray D, Benoist C, Mathis D. Lymphotoxin pathway and Aire influences on thymic medullary epithelial cells are unconnected. J Immunol. 2007;179:5693-700 pubmed
Katsura H, Sontake V, Tata A, Kobayashi Y, Edwards C, Heaton B, et al. Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction. Cell Stem Cell. 2020;27:890-904.e8 pubmed publisher
Wen X, Zhang J, Wan X, Frank A, Qu X, Kellum J. Tissue Inhibitor of Metalloproteinases-2 Mediates Kidney Injury during Sepsis. Nephron. 2020;144:644-649 pubmed publisher
Cousins F, Farley J, Kerrigan R, Mukherjee S, Darzi S, Gargett C, et al. The effects of hedgehog ligand neutralising antibody 5E1 in a mouse model of endometriosis. BMC Res Notes. 2020;13:454 pubmed publisher
Remmerie A, Martens L, Thoné T, Castoldi A, Seurinck R, Pavie B, et al. Osteopontin Expression Identifies a Subset of Recruited Macrophages Distinct from Kupffer Cells in the Fatty Liver. Immunity. 2020;53:641-657.e14 pubmed publisher
Ko H, Hong S, Verma R, Jung J, Lee M, Kim N, et al. Dietary Glucose Consumption Promotes RALDH Activity in Small Intestinal CD103+CD11b+ Dendritic Cells. Front Immunol. 2020;11:1897 pubmed publisher
Wang X, Yang L, Wang Y, Xu Z, Feng Y, Zhang J, et al. Comparative analysis of cell lineage differentiation during hepatogenesis in humans and mice at the single-cell transcriptome level. Cell Res. 2020;30:1109-1126 pubmed publisher
Kuroki S, Maeda R, Yano M, Kitano S, Miyachi H, Fukuda M, et al. H3K9 Demethylases JMJD1A and JMJD1B Control Prospermatogonia to Spermatogonia Transition in Mouse Germline. Stem Cell Reports. 2020;15:424-438 pubmed publisher
Monin L, Ushakov D, Arnesen H, Bah N, Jandke A, Muñoz Ruiz M, et al. γδ T cells compose a developmentally regulated intrauterine population and protect against vaginal candidiasis. Mucosal Immunol. 2020;13:969-981 pubmed publisher
Kazakevych J, Denizot J, Liebert A, Portovedo M, Mosavie M, Jain P, et al. Smarcad1 mediates microbiota-induced inflammation in mouse and coordinates gene expression in the intestinal epithelium. Genome Biol. 2020;21:64 pubmed publisher
Martinez L, Garcia G, Contreras D, Gong D, Sun R, Arumugaswami V. Zika Virus Mucosal Infection Provides Protective Immunity. J Virol. 2020;94: pubmed publisher
Xie Y, Ding F, Di W, Lv Y, Xia F, Sheng Y, et al. Impact of a high‑fat diet on intestinal stem cells and epithelial barrier function in middle‑aged female mice. Mol Med Rep. 2020;21:1133-1144 pubmed publisher
Erlangga Z, Wolff K, Poth T, Peltzer A, Nahnsen S, Spielberg S, et al. Potent Antitumor Activity of Liposomal Irinotecan in an Organoid- and CRISPR-Cas9-Based Murine Model of Gallbladder Cancer. Cancers (Basel). 2019;11: pubmed publisher
Wang J, Wang D, Chu K, Li W, Zeng Y. Procr-expressing progenitor cells are responsible for murine ovulatory rupture repair of ovarian surface epithelium. Nat Commun. 2019;10:4966 pubmed publisher
Pfefferle A, Darr D, Calhoun B, Mott K, Rosen J, Perou C. The MMTV-Wnt1 murine model produces two phenotypically distinct subtypes of mammary tumors with unique therapeutic responses to an EGFR inhibitor. Dis Model Mech. 2019;12: pubmed publisher
Wen S, Shim R, Ho L, Wanrooy B, Srikhanta Y, Prame Kumar K, et al. Advanced age promotes colonic dysfunction and gut-derived lung infection after stroke. Aging Cell. 2019;18:e12980 pubmed publisher
Zhao G, Wei X, Wu J, Eichele D, Lele S, Yang L, et al. A Macromolecular Janus Kinase (JAK) Inhibitor Prodrug Effectively Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice. Pharm Res. 2019;36:64 pubmed publisher
Jones J, Brindley C, Elder N, Myers M, Rajala M, Dekaney C, et al. Cellular Plasticity of Defa4Cre-Expressing Paneth Cells in Response to Notch Activation and Intestinal Injury. Cell Mol Gastroenterol Hepatol. 2019;7:533-554 pubmed publisher
Bartoschek M, Oskolkov N, Bocci M, Lövrot J, Larsson C, Sommarin M, et al. Spatially and functionally distinct subclasses of breast cancer-associated fibroblasts revealed by single cell RNA sequencing. Nat Commun. 2018;9:5150 pubmed publisher
Glal D, Sudhakar J, Lu H, Liu M, Chiang H, Liu Y, et al. ATF3 Sustains IL-22-Induced STAT3 Phosphorylation to Maintain Mucosal Immunity Through Inhibiting Phosphatases. Front Immunol. 2018;9:2522 pubmed publisher
Adam L, Lopez Gonzalez M, Björk A, Pålsson S, Poux C, Wahren Herlenius M, et al. Early Resistance of Non-virulent Mycobacterial Infection in C57BL/6 Mice Is Associated With Rapid Up-Regulation of Antimicrobial Cathelicidin Camp. Front Immunol. 2018;9:1939 pubmed publisher
Moretti F, Klapproth S, Ruppert R, Margraf A, Weber J, Pick R, et al. Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus. elife. 2018;7: pubmed publisher
Jenkins S, Nima Z, Vang K, Kannarpady G, Nedosekin D, Zharov V, et al. Triple-negative breast cancer targeting and killing by EpCAM-directed, plasmonically active nanodrug systems. NPJ Precis Oncol. 2017;1:27 pubmed publisher
Bockerstett K, Wong C, Koehm S, Ford E, DiPaolo R. Molecular Characterization of Gastric Epithelial Cells Using Flow Cytometry. Int J Mol Sci. 2018;19: pubmed publisher
Lopes N, Charaix J, Cedile O, Serge A, Irla M. Lymphotoxin α fine-tunes T cell clonal deletion by regulating thymic entry of antigen-presenting cells. Nat Commun. 2018;9:1262 pubmed publisher
Hosseini Z, Nelson D, Moskwa N, Sfakis L, Castracane J, Larsen M. FGF2-dependent mesenchyme and laminin-111 are niche factors in salivary gland organoids. J Cell Sci. 2018;131: pubmed publisher
Koh A, Miller E, Buenrostro J, Moskowitz D, Wang J, Greenleaf W, et al. Rapid chromatin repression by Aire provides precise control of immune tolerance. Nat Immunol. 2018;19:162-172 pubmed publisher
Yui S, Azzolin L, Maimets M, Pedersen M, Fordham R, Hansen S, et al. YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration. Cell Stem Cell. 2018;22:35-49.e7 pubmed publisher
Henderson M, Madenspacher J, Whitehead G, Thomas S, Aloor J, Gowdy K, et al. Effects of Orally Ingested Arsenic on Respiratory Epithelial Permeability to Bacteria and Small Molecules in Mice. Environ Health Perspect. 2017;125:097024 pubmed publisher
Nikolaidis N, Noel J, Pitstick L, Gardner J, Uehara Y, Wu H, et al. Mitogenic stimulation accelerates influenza-induced mortality by increasing susceptibility of alveolar type II cells to infection. Proc Natl Acad Sci U S A. 2017;114:E6613-E6622 pubmed publisher
Yu L, Chen S, Luo N, He S. The C-terminus domain of the hepatitis B virus x protein stimulates the proliferation of mouse foetal hepatic progenitor cells, although it is not required for the formation of spheroids. Int J Mol Med. 2017;40:400-410 pubmed publisher
Wu B, An C, Li Y, Yin Z, Gong L, Li Z, et al. Reconstructing Lineage Hierarchies of Mouse Uterus Epithelial Development Using Single-Cell Analysis. Stem Cell Reports. 2017;9:381-396 pubmed publisher
Herriges M, Tischfield D, Cui Z, Morley M, Han Y, Babu A, et al. The NANCI-Nkx2.1 gene duplex buffers Nkx2.1 expression to maintain lung development and homeostasis. Genes Dev. 2017;31:889-903 pubmed publisher
Galani I, Triantafyllia V, Eleminiadou E, Koltsida O, Stavropoulos A, Manioudaki M, et al. Interferon-? Mediates Non-redundant Front-Line Antiviral Protection against Influenza Virus Infection without Compromising Host Fitness. Immunity. 2017;46:875-890.e6 pubmed publisher
Lim J, Ibaseta A, Fischer M, Cancilla B, O Young G, Cristea S, et al. Intratumoural heterogeneity generated by Notch signalling promotes small-cell lung cancer. Nature. 2017;545:360-364 pubmed publisher
Tammela T, Sanchez Rivera F, Cetinbas N, Wu K, Joshi N, Helenius K, et al. A Wnt-producing niche drives proliferative potential and progression in lung adenocarcinoma. Nature. 2017;545:355-359 pubmed publisher
Lopes N, Vachon H, Marie J, Irla M. Administration of RANKL boosts thymic regeneration upon bone marrow transplantation. EMBO Mol Med. 2017;9:835-851 pubmed publisher
Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, et al. DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs. Nature. 2017;543:568-572 pubmed publisher
de Aguiar Vallim T, Lee E, Merriott D, Goulbourne C, Cheng J, Cheng A, et al. ABCG1 regulates pulmonary surfactant metabolism in mice and men. J Lipid Res. 2017;58:941-954 pubmed publisher
Ohlund D, Handly Santana A, Biffi G, Elyada E, Almeida A, Ponz Sarvisé M, et al. Distinct populations of inflammatory fibroblasts and myofibroblasts in pancreatic cancer. J Exp Med. 2017;214:579-596 pubmed publisher
Mohapatra B, Zutshi N, An W, Goetz B, Arya P, Bielecki T, et al. An essential role of CBL and CBL-B ubiquitin ligases in mammary stem cell maintenance. Development. 2017;144:1072-1086 pubmed publisher
Liu Z, Guo W, Zhang D, Pang Y, Shi J, Wan S, et al. Circulating tumor cell detection in hepatocellular carcinoma based on karyoplasmic ratios using imaging flow cytometry. Sci Rep. 2016;6:39808 pubmed publisher
Connor L, Tang S, Cognard E, Ochiai S, Hilligan K, Old S, et al. Th2 responses are primed by skin dendritic cells with distinct transcriptional profiles. J Exp Med. 2017;214:125-142 pubmed publisher
Wu X, Briseño C, Durai V, Albring J, Haldar M, Bagadia P, et al. Mafb lineage tracing to distinguish macrophages from other immune lineages reveals dual identity of Langerhans cells. J Exp Med. 2016;213:2553-2565 pubmed
Snitow M, Lu M, Cheng L, Zhou S, Morrisey E. Ezh2 restricts the smooth muscle lineage during mouse lung mesothelial development. Development. 2016;143:3733-3741 pubmed
Shi Y, Wu W, Chai Q, Li Q, Hou Y, Xia H, et al. LTβR controls thymic portal endothelial cells for haematopoietic progenitor cell homing and T-cell regeneration. Nat Commun. 2016;7:12369 pubmed publisher
Gieseck R, Ramalingam T, Hart K, Vannella K, Cantu D, Lu W, et al. Interleukin-13 Activates Distinct Cellular Pathways Leading to Ductular Reaction, Steatosis, and Fibrosis. Immunity. 2016;45:145-58 pubmed publisher
Wu H, Choi S, Levitt P. Differential patterning of genes involved in serotonin metabolism and transport in extra-embryonic tissues of the mouse. Placenta. 2016;42:74-83 pubmed publisher
Wang N, Dong B, Quan Y, Chen Q, Chu M, Xu J, et al. Regulation of Prostate Development and Benign Prostatic Hyperplasia by Autocrine Cholinergic Signaling via Maintaining the Epithelial Progenitor Cells in Proliferating Status. Stem Cell Reports. 2016;6:668-678 pubmed publisher
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Lee C, Penberthy K, Wheeler K, Juncadella I, Vandenabeele P, Lysiak J, et al. Boosting Apoptotic Cell Clearance by Colonic Epithelial Cells Attenuates Inflammation In Vivo. Immunity. 2016;44:807-20 pubmed publisher
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Satoh R, Kakugawa K, Yasuda T, Yoshida H, Sibilia M, Katsura Y, et al. Requirement of Stat3 Signaling in the Postnatal Development of Thymic Medullary Epithelial Cells. PLoS Genet. 2016;12:e1005776 pubmed publisher
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product information
Product Type :
Antibody
Product Name :
CD326 (EpCAM) Monoclonal Antibody (G8.8), eBioscience
Catalog # :
14-5791-81
Quantity :
50 µg
Price :
US 79.25
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.125 µg/test, Immunohistochemistry (Frozen): Assay-Dependent, Immunoprecipitation: Assay-Dependent
Species :
Mouse
Clone :
G8.8
Isotype :
IgG2a, kappa
Storage :
4° C
Description :
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is highly expressed in human carcinomas, and is a marker for tumors of epithelial lineage. Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and many carcinoma types. Also, Ep-CAM reportedly distinguishes adenocarcinomas from pleural mesotheliomas.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.125 µg/test, Immunohistochemistry (Frozen): Assay-Dependent, Immunoprecipitation: Assay-Dependent
Aliases :
adenocarcinoma-associated antigen; CD326; cell surface glycoprotein Trop-1; DIAR5; EGP; EGP-2; EGP314; EGP40; EPCAM; Ep-CAM; EpCAM1; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein; Epithelial glycoprotein 314; ESA; GA733-2; gp40; hEGP314; HNPCC8; human epithelial glycoprotein-2; KS 1/4 antigen; KS1/4; KSA; Ly74; lymphocyte antigen 74; M1S2; M4S1; major gastrointestinal tumor-associated protein GA733-2; mEGP314; membrane component, chromosome 4, surface marker (35kD glycoprotein); MIC18; MK-1; panepithelial glycoprotein 314; protein 289A; Protein D5.7A; Tacsd1; Tacstd1; TROP1; Trop-1 protein; Tumor-associated calcium signal transducer 1
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA