Nanog Monoclonal Antibody (hNanog.2), eBioscience™ | Invitrogen 14-5768-82 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Nanog Monoclonal Antibody (hNanog.2), eBioscience™

catalog :

14-5768-82

quantity :

100µg

price :

US 305

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

hNanog.2

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:50; fig 1	Momcilovic O, Sivapatham R, Oron T, Meyer M, Mooney S, Rao M, et al. Derivation, Characterization, and Neural Differentiation of Integration-Free Induced Pluripotent Stem Cell Lines from Parkinson's Disease Patients Carrying SNCA, LRRK2, PARK2, and GBA Mutations. PLoS ONE. 2016;11:e0154890 pubmed publisher
immunocytochemistry; human	Sivapatham R, Zeng X. Generation and Characterization of Patient-Specific Induced Pluripotent Stem Cell for Disease Modeling. Methods Mol Biol. 2016;1353:25-44 pubmed publisher
	Brinkhof B, van Tol H, Groot Koerkamp M, Wubbolts R, Haagsman H, Roelen B. Characterization of bovine embryos cultured under conditions appropriate for sustaining human naïve pluripotency. PLoS ONE. 2017;12:e0172920 pubmed publisher
	Jeter C, Liu B, Lu Y, Chao H, Zhang D, Liu X, et al. NANOG reprograms prostate cancer cells to castration resistance via dynamically repressing and engaging the AR/FOXA1 signaling axis. Cell Discov. 2016;2:16041 pubmed
	Brinkhof B, van Tol H, Groot Koerkamp M, Riemers F, IJzer S, Mashayekhi K, et al. A mRNA landscape of bovine embryos after standard and MAPK-inhibited culture conditions: a comparative analysis. BMC Genomics. 2015;16:277 pubmed publisher
	Liu B, Badeaux M, Choy G, Chandra D, Shen I, Jeter C, et al. Nanog1 in NTERA-2 and recombinant NanogP8 from somatic cancer cells adopt multiple protein conformations and migrate at multiple M.W species. PLoS ONE. 2014;9:e90615 pubmed publisher
	Kuijk E, de Gier J, Lopes S, Chambers I, van Pelt A, Colenbrander B, et al. A distinct expression pattern in mammalian testes indicates a conserved role for NANOG in spermatogenesis. PLoS ONE. 2010;5:e10987 pubmed publisher
	Mitsui K, Tokuzawa Y, Itoh H, Segawa K, Murakami M, Takahashi K, et al. The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell. 2003;113:631-42 pubmed
	Cavaleri F, Scholer H. Nanog: a new recruit to the embryonic stem cell orchestra. Cell. 2003;113:551-2 pubmed

product information

Product Type :

Antibody

Product Name :

Nanog Monoclonal Antibody (hNanog.2), eBioscience™

Catalog # :

14-5768-82

Quantity :

100µg

Price :

US 305

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human

Applications :

Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Immunohistochemistry (Paraffin): 20 µg/mL, Western Blot: 1 µg/mL

Species :

Human

Clone :

hNanog.2

Isotype :

IgG1, kappa

Storage :

4° C

Description :

NANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Immunohistochemistry (Paraffin): 20 µg/mL, Western Blot: 1 µg/mL

Aliases :

2410002E02Rik; early embryo specific expression NK family; Early embryo specific expression NK-type homeobox protein; Ecat4; Enk; ES cell-associated protein 4; FLJ12581; HGNC:20857; hNanog; Homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; homeodomain transcription factor Nanog; NANOG; Nanog homeobox; Nanog homeodomain transcription factor

more info or order :

Invitrogen product webpage