| Published Application/Species/Sample/Dilution | Reference |
|---|
- immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 3a
| Xu P, Borges R, Fillatre J, de Oliveira Melo M, Cheng T, Thisse B, et al. Construction of a mammalian embryo model from stem cells organized by a morphogen signalling centre. Nat Commun. 2021;12:3277 pubmed publisher |
- immunocytochemistry; mouse; 1:1000; loading ...; fig 1d
| Yamane M, Ohtsuka S, Matsuura K, Nakamura A, Niwa H. Overlapping functions of Krüppel-like factor family members: targeting multiple transcription factors to maintain the naïve pluripotency of mouse embryonic stem cells. Development. 2018;145: pubmed publisher |
- immunocytochemistry; mouse; 1:200; loading ...; fig 3f
| Li M, Amaral P, Cheung P, Bergmann J, Kinoshita M, Kalkan T, et al. A lncRNA fine tunes the dynamics of a cell state transition involving Lin28, let-7 and de novo DNA methylation. elife. 2017;6: pubmed publisher |
- immunocytochemistry; mouse; 1:800; loading ...; fig 3e
| Jang S, Choubey S, Furchtgott L, Zou L, Doyle A, Menon V, et al. Dynamics of embryonic stem cell differentiation inferred from single-cell transcriptomics show a series of transitions through discrete cell states. elife. 2017;6: pubmed publisher |
- immunocytochemistry; mouse; 2500 ng/ml; fig 3
| Illingworth R, Hölzenspies J, Roske F, Bickmore W, Brickman J. Polycomb enables primitive endoderm lineage priming in embryonic stem cells. elife. 2016;5: pubmed publisher |
- immunohistochemistry; mouse; 1:200; loading ...; fig 1b
| Thamodaran V, Bruce A. p38 (Mapk14/11) occupies a regulatory node governing entry into primitive endoderm differentiation during preimplantation mouse embryo development. Open Biol. 2016;6: pubmed publisher |
- immunocytochemistry; mouse; 1:200; fig 2
- immunohistochemistry; mouse; 1:200; fig 3
| Martin Gonzalez J, Morgani S, Bone R, Bonderup K, Abelchian S, Brakebusch C, et al. Embryonic Stem Cell Culture Conditions Support Distinct States Associated with Different Developmental Stages and Potency. Stem Cell Reports. 2016;7:177-91 pubmed publisher |
- western blot; mouse; 1:500; fig 3
| Murakami K, Günesdogan U, Zylicz J, Tang W, Sengupta R, Kobayashi T, et al. NANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers. Nature. 2016;529:403-407 pubmed publisher |
- immunohistochemistry; mouse; 1:100; fig 1
| Alexandrova S, Kalkan T, Humphreys P, Riddell A, Scognamiglio R, Trumpp A, et al. Selection and dynamics of embryonic stem cell integration into early mouse embryos. Development. 2016;143:24-34 pubmed publisher |
- immunocytochemistry; mouse; loading ...; fig 3d
| Higuchi Y, Nguyen C, Yasuda S, McMillan M, Hasegawa K, Kahn M. Specific Direct Small Molecule p300/?-Catenin Antagonists Maintain Stem Cell Potency. Curr Mol Pharmacol. 2016;9:272-279 pubmed |
- immunocytochemistry; mouse; loading ...; fig s5a
| Nakamura T, Yabuta Y, Okamoto I, Aramaki S, Yokobayashi S, Kurimoto K, et al. SC3-seq: a method for highly parallel and quantitative measurement of single-cell gene expression. Nucleic Acids Res. 2015;43:e60 pubmed publisher |
- immunohistochemistry; mouse; 1:500
| Bangs F, Schrode N, Hadjantonakis A, Anderson K. Lineage specificity of primary cilia in the mouse embryo. Nat Cell Biol. 2015;17:113-22 pubmed publisher |
- immunohistochemistry - paraffin section; mouse
| Geula S, Moshitch Moshkovitz S, Dominissini D, Mansour A, Kol N, Salmon Divon M, et al. Stem cells. m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation. Science. 2015;347:1002-6 pubmed publisher |
- immunocytochemistry; mouse
| Gouti M, Tsakiridis A, Wymeersch F, Huang Y, Kleinjung J, Wilson V, et al. In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity. PLoS Biol. 2014;12:e1001937 pubmed publisher |
- immunocytochemistry; mouse; 1:200
| Doughton G, Wei J, Tapon N, Welham M, Chalmers A. Formation of a polarised primitive endoderm layer in embryoid bodies requires fgfr/erk signalling. PLoS ONE. 2014;9:e95434 pubmed publisher |
- immunocytochemistry; mouse; 1:500; fig 7
- immunohistochemistry; mouse; 1:500; fig 4
| Betschinger J, Nichols J, Dietmann S, Corrin P, Paddison P, Smith A. Exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor Tfe3. Cell. 2013;153:335-47 pubmed publisher |
| Kinoshita M, Barber M, Mansfield W, Cui Y, Spindlow D, Stirparo G, et al. Capture of Mouse and Human Stem Cells with Features of Formative Pluripotency. Cell Stem Cell. 2021;28:453-471.e8 pubmed publisher |
| Bergert M, Lembo S, Sharma S, Russo L, Milovanović D, Gretarsson K, et al. Cell Surface Mechanics Gate Embryonic Stem Cell Differentiation. Cell Stem Cell. 2021;28:209-216.e4 pubmed publisher |
| Yamamoto M, Suwa Y, Sugiyama K, Okashita N, Kawaguchi M, Tani N, et al. PRDM14-CtBP1/2-PRC2 complex regulates transcriptional repression during transition from primed to naïve pluripotency. J Cell Sci. 2020;: pubmed publisher |
| Ochiai H, Hayashi T, Umeda M, Yoshimura M, Harada A, Shimizu Y, et al. Genome-wide kinetic properties of transcriptional bursting in mouse embryonic stem cells. Sci Adv. 2020;6:eaaz6699 pubmed publisher |
| Mulas C, Hodgson A, Kohler T, Agley C, Humphreys P, Kleine Brüggeney H, et al. Microfluidic platform for 3D cell culture with live imaging and clone retrieval. Lab Chip. 2020;20:2580-2591 pubmed publisher |
| Rao C, Malaguti M, Mason J, Lowell S. The transcription factor E2A drives neural differentiation in pluripotent cells. Development. 2020;147: pubmed publisher |
| Fischer S, Corujo Simon E, Lilao Garzon J, Stelzer E, Muñoz Descalzo S. The transition from local to global patterns governs the differentiation of mouse blastocysts. PLoS ONE. 2020;15:e0233030 pubmed publisher |
| Velychko S, Adachi K, Kim K, Hou Y, MacCarthy C, Wu G, et al. Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental Potential of iPSCs. Cell Stem Cell. 2019;25:737-753.e4 pubmed publisher |
| Li R, Zhong C, Yu Y, Liu H, Sakurai M, Yu L, et al. Generation of Blastocyst-like Structures from Mouse Embryonic and Adult Cell Cultures. Cell. 2019;179:687-702.e18 pubmed publisher |
| Pantier R, Tatar T, Colby D, Chambers I. Endogenous epitope-tagging of Tet1, Tet2 and Tet3 identifies TET2 as a naïve pluripotency marker. Life Sci Alliance. 2019;2: pubmed publisher |
| Vanheer L, Song J, De Geest N, Janiszewski A, Talon I, Provenzano C, et al. Tox4 modulates cell fate reprogramming. J Cell Sci. 2019;132: pubmed publisher |
| Vardhana S, Arnold P, Rosen B, Chen Y, Carey B, Huangfu D, et al. Glutamine independence is a selectable feature of pluripotent stem cells. Nat Metab. 2019;1:676-687 pubmed publisher |
| Stuart H, Stirparo G, Lohoff T, Bates L, Kinoshita M, Lim C, et al. Distinct Molecular Trajectories Converge to Induce Naive Pluripotency. Cell Stem Cell. 2019;25:388-406.e8 pubmed publisher |
| Lawrence M, Theunissen T, Lombard P, Adams D, Silva J. ZMYM2 inhibits NANOG-mediated reprogramming. Wellcome Open Res. 2019;4:88 pubmed publisher |
| Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, et al. Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive Pluripotency. Cell Stem Cell. 2018;23:266-275.e6 pubmed publisher |
| Finley L, Vardhana S, Carey B, Alonso Curbelo D, Koche R, Chen Y, et al. Pluripotency transcription factors and Tet1/2 maintain Brd4-independent stem cell identity. Nat Cell Biol. 2018;20:565-574 pubmed publisher |
| Cao K, Collings C, Morgan M, Marshall S, Rendleman E, Ozark P, et al. An Mll4/COMPASS-Lsd1 epigenetic axis governs enhancer function and pluripotency transition in embryonic stem cells. Sci Adv. 2018;4:eaap8747 pubmed publisher |
| Yachie Kinoshita A, Onishi K, Ostblom J, Langley M, Pósfai E, Rossant J, et al. Modeling signaling-dependent pluripotency with Boolean logic to predict cell fate transitions. Mol Syst Biol. 2018;14:e7952 pubmed publisher |
| Corsinotti A, Wong F, Tatar T, Szczerbinska I, Halbritter F, Colby D, et al. Distinct SoxB1 networks are required for naïve and primed pluripotency. elife. 2017;6: pubmed publisher |
| Ju Lee H, Bartsch D, Xiao C, Guerrero S, Ahuja G, Schindler C, et al. A post-transcriptional program coordinated by CSDE1 prevents intrinsic neural differentiation of human embryonic stem cells. Nat Commun. 2017;8:1456 pubmed publisher |
| Bessonnard S, Coqueran S, Vandormael Pournin S, Dufour A, Artus J, Cohen Tannoudji M. ICM conversion to epiblast by FGF/ERK inhibition is limited in time and requires transcription and protein degradation. Sci Rep. 2017;7:12285 pubmed publisher |
| Nissen S, Perera M, Gonzalez J, Morgani S, Jensen M, Sneppen K, et al. Four simple rules that are sufficient to generate the mammalian blastocyst. PLoS Biol. 2017;15:e2000737 pubmed publisher |
| Chronis C, Fiziev P, Papp B, Butz S, Bonora G, Sabri S, et al. Cooperative Binding of Transcription Factors Orchestrates Reprogramming. Cell. 2017;168:442-459.e20 pubmed publisher |
| Jerabek S, Ng C, Wu G, Arauzo Bravo M, Kim K, Esch D, et al. Changing POU dimerization preferences converts Oct6 into a pluripotency inducer. EMBO Rep. 2017;18:319-333 pubmed publisher |
| Schroter C, Rué P, Mackenzie J, Martinez Arias A. FGF/MAPK signaling sets the switching threshold of a bistable circuit controlling cell fate decisions in embryonic stem cells. Development. 2015;142:4205-16 pubmed publisher |
| Economou C, Tsakiridis A, Wymeersch F, Gordon Keylock S, Dewhurst R, Fisher D, et al. Intrinsic factors and the embryonic environment influence the formation of extragonadal teratomas during gestation. BMC Dev Biol. 2015;15:35 pubmed publisher |
| Morgani S, Brickman J. LIF supports primitive endoderm expansion during pre-implantation development. Development. 2015;142:3488-99 pubmed publisher |
| Chantzoura E, Skylaki S, Menendez S, Kim S, Johnsson A, Linnarsson S, et al. Reprogramming Roadblocks Are System Dependent. Stem Cell Reports. 2015;5:350-64 pubmed publisher |
| Pasque V, Tchieu J, Karnik R, Uyeda M, Sadhu Dimashkie A, Case D, et al. X chromosome reactivation dynamics reveal stages of reprogramming to pluripotency. Cell. 2014;159:1681-97 pubmed publisher |
| Turner D, Rué P, Mackenzie J, Davies E, Martinez Arias A. Brachyury cooperates with Wnt/β-catenin signalling to elicit primitive-streak-like behaviour in differentiating mouse embryonic stem cells. BMC Biol. 2014;12:63 pubmed publisher |
| Tsakiridis A, Huang Y, Blin G, Skylaki S, Wymeersch F, Osorno R, et al. Distinct Wnt-driven primitive streak-like populations reflect in vivo lineage precursors. Development. 2014;141:1209-21 pubmed publisher |
| Le Bin G, Muñoz Descalzo S, Kurowski A, Leitch H, Lou X, Mansfield W, et al. Oct4 is required for lineage priming in the developing inner cell mass of the mouse blastocyst. Development. 2014;141:1001-10 pubmed publisher |
| Huijbers I, Bin Ali R, Pritchard C, Cozijnsen M, Kwon M, Proost N, et al. Rapid target gene validation in complex cancer mouse models using re-derived embryonic stem cells. EMBO Mol Med. 2014;6:212-25 pubmed publisher |
| Malaguti M, Nistor P, Blin G, Pegg A, Zhou X, Lowell S. Bone morphogenic protein signalling suppresses differentiation of pluripotent cells by maintaining expression of E-Cadherin. elife. 2013;2:e01197 pubmed publisher |
| Payer B, Rosenberg M, Yamaji M, Yabuta Y, Koyanagi Aoi M, Hayashi K, et al. Tsix RNA and the germline factor, PRDM14, link X reactivation and stem cell reprogramming. Mol Cell. 2013;52:805-18 pubmed publisher |
| Denholtz M, Bonora G, Chronis C, Splinter E, de Laat W, Ernst J, et al. Long-range chromatin contacts in embryonic stem cells reveal a role for pluripotency factors and polycomb proteins in genome organization. Cell Stem Cell. 2013;13:602-16 pubmed publisher |
| Kumar R, Dimenna L, Schrode N, Liu T, Franck P, Muñoz Descalzo S, et al. AID stabilizes stem-cell phenotype by removing epigenetic memory of pluripotency genes. Nature. 2013;500:89-92 pubmed publisher |
| Morgani S, Canham M, Nichols J, Sharov A, Migueles R, Ko M, et al. Totipotent embryonic stem cells arise in ground-state culture conditions. Cell Rep. 2013;3:1945-57 pubmed publisher |
| Radzisheuskaya A, Chia G, Dos Santos R, Theunissen T, Castro L, Nichols J, et al. A defined Oct4 level governs cell state transitions of pluripotency entry and differentiation into all embryonic lineages. Nat Cell Biol. 2013;15:579-90 pubmed publisher |
| Faunes F, Hayward P, Descalzo S, Chatterjee S, Balayo T, Trott J, et al. A membrane-associated β-catenin/Oct4 complex correlates with ground-state pluripotency in mouse embryonic stem cells. Development. 2013;140:1171-83 pubmed publisher |
| Pasque V, Radzisheuskaya A, Gillich A, Halley Stott R, Panamarova M, Zernicka Goetz M, et al. Histone variant macroH2A marks embryonic differentiation in vivo and acts as an epigenetic barrier to induced pluripotency. J Cell Sci. 2012;125:6094-104 pubmed publisher |
| Muñoz Descalzo S, Rué P, Garcia Ojalvo J, Martinez Arias A. Correlations between the levels of Oct4 and Nanog as a signature for naïve pluripotency in mouse embryonic stem cells. Stem Cells. 2012;30:2683-91 pubmed publisher |
| Marks H, Kalkan T, Menafra R, Denissov S, Jones K, Hofemeister H, et al. The transcriptional and epigenomic foundations of ground state pluripotency. Cell. 2012;149:590-604 pubmed publisher |
| Wray J, Kalkan T, Gómez López S, Eckardt D, Cook A, Kemler R, et al. Inhibition of glycogen synthase kinase-3 alleviates Tcf3 repression of the pluripotency network and increases embryonic stem cell resistance to differentiation. Nat Cell Biol. 2011;13:838-45 pubmed publisher |
| Guo G, Huang Y, Humphreys P, Wang X, Smith A. A PiggyBac-based recessive screening method to identify pluripotency regulators. PLoS ONE. 2011;6:e18189 pubmed publisher |
| Wang Y, Fan Y, Puga A. Dioxin exposure disrupts the differentiation of mouse embryonic stem cells into cardiomyocytes. Toxicol Sci. 2010;115:225-37 pubmed publisher |
| Chen Y, Du Z, Yao Z. Roles of the Nanog protein in murine F9 embryonal carcinoma cells and their endoderm-differentiated counterparts. Cell Res. 2006;16:641-50 pubmed |
| Pan G, Pei D. The stem cell pluripotency factor NANOG activates transcription with two unusually potent subdomains at its C terminus. J Biol Chem. 2005;280:1401-7 pubmed |
| Mitsui K, Tokuzawa Y, Itoh H, Segawa K, Murakami M, Takahashi K, et al. The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell. 2003;113:631-42 pubmed |
