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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Nanog Monoclonal Antibody (eBioMLC-51), eBioscience™
catalog :
14-5761-37
quantity :
2 mg
price :
1200.00 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
eBioMLC-51
reactivity :
mouse
application :
western blot, immunocytochemistry
more info or order :
citations: 44
Reference
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Finley L, Vardhana S, Carey B, Alonso Curbelo D, Koche R, Chen Y, et al. Pluripotency transcription factors and Tet1/2 maintain Brd4-independent stem cell identity. Nat Cell Biol. 2018;20:565-574 pubmed publisher
Cao K, Collings C, Morgan M, Marshall S, Rendleman E, Ozark P, et al. An Mll4/COMPASS-Lsd1 epigenetic axis governs enhancer function and pluripotency transition in embryonic stem cells. Sci Adv. 2018;4:eaap8747 pubmed publisher
Yachie Kinoshita A, Onishi K, Ostblom J, Langley M, Pósfai E, Rossant J, et al. Modeling signaling-dependent pluripotency with Boolean logic to predict cell fate transitions. Mol Syst Biol. 2018;14:e7952 pubmed publisher
Corsinotti A, Wong F, Tatar T, Szczerbinska I, Halbritter F, Colby D, et al. Distinct SoxB1 networks are required for naïve and primed pluripotency. elife. 2017;6: pubmed publisher
Ju Lee H, Bartsch D, Xiao C, Guerrero S, Ahuja G, Schindler C, et al. A post-transcriptional program coordinated by CSDE1 prevents intrinsic neural differentiation of human embryonic stem cells. Nat Commun. 2017;8:1456 pubmed publisher
Bessonnard S, Coqueran S, Vandormael Pournin S, Dufour A, Artus J, Cohen Tannoudji M. ICM conversion to epiblast by FGF/ERK inhibition is limited in time and requires transcription and protein degradation. Sci Rep. 2017;7:12285 pubmed publisher
Li M, Amaral P, Cheung P, Bergmann J, Kinoshita M, Kalkan T, et al. A lncRNA fine tunes the dynamics of a cell state transition involving Lin28, let-7 and de novo DNA methylation. elife. 2017;6: pubmed publisher
Nissen S, Perera M, Gonzalez J, Morgani S, Jensen M, Sneppen K, et al. Four simple rules that are sufficient to generate the mammalian blastocyst. PLoS Biol. 2017;15:e2000737 pubmed publisher
Jang S, Choubey S, Furchtgott L, Zou L, Doyle A, Menon V, et al. Dynamics of embryonic stem cell differentiation inferred from single-cell transcriptomics show a series of transitions through discrete cell states. elife. 2017;6: pubmed publisher
Chronis C, Fiziev P, Papp B, Butz S, Bonora G, Sabri S, et al. Cooperative Binding of Transcription Factors Orchestrates Reprogramming. Cell. 2017;168:442-459.e20 pubmed publisher
Jerabek S, Ng C, Wu G, Arauzo Bravo M, Kim K, Esch D, et al. Changing POU dimerization preferences converts Oct6 into a pluripotency inducer. EMBO Rep. 2017;18:319-333 pubmed publisher
Illingworth R, Hölzenspies J, Roske F, Bickmore W, Brickman J. Polycomb enables primitive endoderm lineage priming in embryonic stem cells. elife. 2016;5: pubmed publisher
Thamodaran V, Bruce A. p38 (Mapk14/11) occupies a regulatory node governing entry into primitive endoderm differentiation during preimplantation mouse embryo development. Open Biol. 2016;6: pubmed publisher
Martin Gonzalez J, Morgani S, Bone R, Bonderup K, Abelchian S, Brakebusch C, et al. Embryonic Stem Cell Culture Conditions Support Distinct States Associated with Different Developmental Stages and Potency. Stem Cell Reports. 2016;7:177-91 pubmed publisher
Murakami K, Günesdogan U, Zylicz J, Tang W, Sengupta R, Kobayashi T, et al. NANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers. Nature. 2016;529:403-407 pubmed publisher
Alexandrova S, Kalkan T, Humphreys P, Riddell A, Scognamiglio R, Trumpp A, et al. Selection and dynamics of embryonic stem cell integration into early mouse embryos. Development. 2016;143:24-34 pubmed publisher
Schroter C, Rué P, Mackenzie J, Martinez Arias A. FGF/MAPK signaling sets the switching threshold of a bistable circuit controlling cell fate decisions in embryonic stem cells. Development. 2015;142:4205-16 pubmed publisher
Economou C, Tsakiridis A, Wymeersch F, Gordon Keylock S, Dewhurst R, Fisher D, et al. Intrinsic factors and the embryonic environment influence the formation of extragonadal teratomas during gestation. BMC Dev Biol. 2015;15:35 pubmed publisher
Morgani S, Brickman J. LIF supports primitive endoderm expansion during pre-implantation development. Development. 2015;142:3488-99 pubmed publisher
Chantzoura E, Skylaki S, Menendez S, Kim S, Johnsson A, Linnarsson S, et al. Reprogramming Roadblocks Are System Dependent. Stem Cell Reports. 2015;5:350-64 pubmed publisher
Higuchi Y, Nguyen C, Yasuda S, McMillan M, Hasegawa K, Kahn M. Specific Direct Small Molecule p300/?-Catenin Antagonists Maintain Stem Cell Potency. Curr Mol Pharmacol. 2016;9:272-279 pubmed
Nakamura T, Yabuta Y, Okamoto I, Aramaki S, Yokobayashi S, Kurimoto K, et al. SC3-seq: a method for highly parallel and quantitative measurement of single-cell gene expression. Nucleic Acids Res. 2015;43:e60 pubmed publisher
Bangs F, Schrode N, Hadjantonakis A, Anderson K. Lineage specificity of primary cilia in the mouse embryo. Nat Cell Biol. 2015;17:113-22 pubmed publisher
Pasque V, Tchieu J, Karnik R, Uyeda M, Sadhu Dimashkie A, Case D, et al. X chromosome reactivation dynamics reveal stages of reprogramming to pluripotency. Cell. 2014;159:1681-97 pubmed publisher
Gouti M, Tsakiridis A, Wymeersch F, Huang Y, Kleinjung J, Wilson V, et al. In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity. PLoS Biol. 2014;12:e1001937 pubmed publisher
Turner D, Rué P, Mackenzie J, Davies E, Martinez Arias A. Brachyury cooperates with Wnt/β-catenin signalling to elicit primitive-streak-like behaviour in differentiating mouse embryonic stem cells. BMC Biol. 2014;12:63 pubmed publisher
Doughton G, Wei J, Tapon N, Welham M, Chalmers A. Formation of a polarised primitive endoderm layer in embryoid bodies requires fgfr/erk signalling. PLoS ONE. 2014;9:e95434 pubmed publisher
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Huijbers I, Bin Ali R, Pritchard C, Cozijnsen M, Kwon M, Proost N, et al. Rapid target gene validation in complex cancer mouse models using re-derived embryonic stem cells. EMBO Mol Med. 2014;6:212-25 pubmed publisher
Malaguti M, Nistor P, Blin G, Pegg A, Zhou X, Lowell S. Bone morphogenic protein signalling suppresses differentiation of pluripotent cells by maintaining expression of E-Cadherin. elife. 2013;2:e01197 pubmed publisher
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Radzisheuskaya A, Chia G, Dos Santos R, Theunissen T, Castro L, Nichols J, et al. A defined Oct4 level governs cell state transitions of pluripotency entry and differentiation into all embryonic lineages. Nat Cell Biol. 2013;15:579-90 pubmed publisher
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product information
Product Type :
Antibody
Product Name :
Nanog Monoclonal Antibody (eBioMLC-51), eBioscience™
Catalog # :
14-5761-37
Quantity :
2 mg
Price (USD) :
1200.00 USD
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
Immunocytochemistry: 2 µg/mL, Immunofluorescence: 2 µg/mL, Western Blot: 2 µg/mL
Species :
Mouse
Clone :
eBioMLC-51
Isotype :
IgG2a, kappa
Storage :
4° C
Description :
NANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 2 µg/mL, Immunofluorescence: 2 µg/mL, Western Blot: 2 µg/mL
Aliases :
2410002E02Rik; early embryo specific expression NK family; early embryo specific expression NK-type homeobox protein; Ecat4; Enk; ES cell-associated protein 4; FLJ12581; HGNC:20857; hNanog; Homeobox protein NANOG; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; homeodomain transcription factor Nanog; NANOG; Nanog homeobox; Nanog homeodomain transcription factor; nanog homeodox protein
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA