Ki-67 Monoclonal Antibody (20Raj1), eBioscience™ | Invitrogen 14-5699-82 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Ki-67 Monoclonal Antibody (20Raj1), eBioscience™

catalog :

14-5699-82

quantity :

100µg

price :

US 139

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

20Raj1

reactivity :

human, mouse, dogs

application :

immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, mass cytometry

more info or order :

Invitrogen product webpage

citations: 26

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 3a	Perciani C, Farah B, Kaul R, Ostrowski M, Mahmud S, Anzala O, et al. Live attenuated varicella-zoster virus vaccine does not induce HIV target cell activation. J Clin Invest. 2019;129:875-886 pubmed publisher
flow cytometry; human; loading ...; fig 4c	Hartana C, Ahlén Bergman E, Broome A, Berglund S, Johansson M, Alamdari F, et al. Tissue-resident memory T cells are epigenetically cytotoxic with signs of exhaustion in human urinary bladder cancer. Clin Exp Immunol. 2018;194:39-53 pubmed publisher
mass cytometry; human; loading ...; fig 2a	Chew V, Lai L, Pan L, Lim C, Li J, Ong R, et al. Delineation of an immunosuppressive gradient in hepatocellular carcinoma using high-dimensional proteomic and transcriptomic analyses. Proc Natl Acad Sci U S A. 2017;114:E5900-E5909 pubmed publisher
immunocytochemistry; human; fig 2b	Christoforou N, Chakraborty S, Kirkton R, Adler A, Addis R, Leong K. Core Transcription Factors, MicroRNAs, and Small Molecules Drive Transdifferentiation of Human Fibroblasts Towards The Cardiac Cell Lineage. Sci Rep. 2017;7:40285 pubmed publisher
flow cytometry; human; loading ...; fig 9h	Goodier M, Rodríguez Galán A, Lusa C, Nielsen C, Darboe A, Moldoveanu A, et al. Influenza Vaccination Generates Cytokine-Induced Memory-like NK Cells: Impact of Human Cytomegalovirus Infection. J Immunol. 2016;197:313-25 pubmed publisher
flow cytometry; human; loading ...; fig 5b	Mitson Salazar A, Yin Y, Wansley D, Young M, Bolan H, Arceo S, et al. Hematopoietic prostaglandin D synthase defines a proeosinophilic pathogenic effector human T(H)2 cell subpopulation with enhanced function. J Allergy Clin Immunol. 2016;137:907-18.e9 pubmed publisher
flow cytometry; human; 1:200; fig s4	Adoro S, Cubillos Ruiz J, Chen X, Deruaz M, Vrbanac V, Song M, et al. IL-21 induces antiviral microRNA-29 in CD4 T cells to limit HIV-1 infection. Nat Commun. 2015;6:7562 pubmed publisher
flow cytometry; human; fig s3	Tsai C, Liong K, Gunalan M, Li N, Lim D, Fisher D, et al. Type I IFNs and IL-18 regulate the antiviral response of primary human Î³Î´ T cells against dendritic cells infected with Dengue virus. J Immunol. 2015;194:3890-900 pubmed publisher
flow cytometry; human; fig 4	Khan A, Srivastava R, Spencer D, Garg S, Fremgen D, Vahed H, et al. Phenotypic and functional characterization of herpes simplex virus glycoprotein B epitope-specific effector and memory CD8+ T cells from symptomatic and asymptomatic individuals with ocular herpes. J Virol. 2015;89:3776-92 pubmed publisher
flow cytometry; human	Bending D, Pesenacker A, Ursu S, Wu Q, Lom H, Thirugnanabalan B, et al. Hypomethylation at the regulatory T cell-specific demethylated region in CD25hi T cells is decoupled from FOXP3 expression at the inflamed site in childhood arthritis. J Immunol. 2014;193:2699-708 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:500	Yuan J, Zhang D, Wang L, Liu M, Mao J, Yin Y, et al. No evidence for neo-oogenesis may link to ovarian senescence in adult monkey. Stem Cells. 2013;31:2538-50 pubmed publisher
	Nguyen H, Noguchi S, Sugie K, Matsuo Y, Nguyen C, Koito H, et al. Small-Vessel Vasculopathy Due to Aberrant Autophagy in LAMP-2 Deficiency. Sci Rep. 2018;8:3326 pubmed publisher
	Titanji K, Vunnava A, Foster A, Sheth A, Lennox J, Knezevic A, et al. T-cell receptor activator of nuclear factor-κB ligand/osteoprotegerin imbalance is associated with HIV-induced bone loss in patients with higher CD4+ T-cell counts. AIDS. 2018;32:885-894 pubmed publisher
	Meinicke H, Bremser A, Brack M, Akeus P, Pearson C, Bullers S, et al. Tumour-associated changes in intestinal epithelial cells cause local accumulation of KLRG1+ GATA3+ regulatory T cells in mice. Immunology. 2017;152:74-88 pubmed publisher
	Wang X, Chen H, Tian R, Zhang Y, Drutskaya M, Wang C, et al. Macrophages induce AKT/β-catenin-dependent Lgr5+ stem cell activation and hair follicle regeneration through TNF. Nat Commun. 2017;8:14091 pubmed publisher
	Perdu S, Castellana B, Kim Y, Chan K, DeLuca L, Beristain A. Maternal obesity drives functional alterations in uterine NK cells. JCI Insight. 2016;1:e85560 pubmed publisher
	Lerchenmüller C, Heißenberg J, Damilano F, Bezzeridis V, Krämer I, Bochaton Piallat M, et al. S100A6 Regulates Endothelial Cell Cycle Progression by Attenuating Antiproliferative Signal Transducers and Activators of Transcription 1 Signaling. Arterioscler Thromb Vasc Biol. 2016;36:1854-67 pubmed publisher
	Zhao Y, Masiello D, McMillian M, Nguyen C, Wu Y, Melendez E, et al. CBP/catenin antagonist safely eliminates drug-resistant leukemia-initiating cells. Oncogene. 2016;35:3705-17 pubmed publisher
	Wang Y, Lanzoni G, Carpino G, Cui C, Dominguez Bendala J, Wauthier E, et al. Biliary tree stem cells, precursors to pancreatic committed progenitors: evidence for possible life-long pancreatic organogenesis. Stem Cells. 2013;31:1966-79 pubmed publisher
	Labuda L, Ateba Ngoa U, Feugap E, Heeringa J, van der Vlugt L, Pires R, et al. Alterations in peripheral blood B cell subsets and dynamics of B cell responses during human schistosomiasis. PLoS Negl Trop Dis. 2013;7:e2094 pubmed publisher
	Breitkopf T, Lo B, Leung G, Wang E, Yu M, Carr N, et al. Somatostatin expression in human hair follicles and its potential role in immune privilege. J Invest Dermatol. 2013;133:1722-30 pubmed publisher
	Billiard F, Lobry C, Darrasse Jèze G, Waite J, Liu X, Mouquet H, et al. Dll4-Notch signaling in Flt3-independent dendritic cell development and autoimmunity in mice. J Exp Med. 2012;209:1011-28 pubmed publisher
	Heidebrecht H, Buck F, Endl E, Kruse M, Adam Klages S, Andersen K, et al. Ki-Mcm6, a new monoclonal antibody specific to Mcm6: comparison of the distribution profile of Mcm6 and the Ki-67 antigen. Lab Invest. 2001;81:1163-5 pubmed
	Rishi M, Schwarting R, Kovatich A, Ehya H. Detection of growth fraction in tumors by Ki67 monoclonal antibody in cytologic smears: a prospective study of 40 cases. Diagn Cytopathol. 1993;9:52-6; dicussion 57-8 pubmed
	Schluter C, Duchrow M, Wohlenberg C, Becker M, Key G, Flad H, et al. The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins. J Cell Biol. 1993;123:513-22 pubmed
	Mooy C, de Jong P, Van der Kwast T, Mulder P, Jager M, Ruiter D. Ki-67 immunostaining in uveal melanoma. The effect of pre-enucleation radiotherapy. Ophthalmology. 1990;97:1275-80 pubmed

product information

Product Type :

Antibody

Product Name :

Ki-67 Monoclonal Antibody (20Raj1), eBioscience™

Catalog # :

14-5699-82

Quantity :

100µg

Price :

US 139

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Canine, Human

Applications :

Immunocytochemistry: 0.5-1 µg/mL, Immunofluorescence: 0.5-1 µg/mL, Immunohistochemistry (Paraffin): 0.5-1 µg/mL

Species :

Canine, Human

Clone :

20Raj1

Isotype :

IgG1, kappa

Storage :

4° C

Description :

Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 0.5-1 µg/mL, Immunofluorescence: 0.5-1 µg/mL, Immunohistochemistry (Paraffin): 0.5-1 µg/mL

Aliases :

antigen identified by monoclonal antibody Ki 67; Antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; antigen KI-67-like; cb31; D630048A14Rik; I79_022666; Ki67; Ki-67; KIA; LOW QUALITY PROTEIN: proliferation marker protein Ki-67; marker of proliferation Ki-67; MIB-; MIB-1; Mki67; PPP1R105; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; sb:cb31; si:ch211-250b22.7; wu:fa11g09; wu:fb57a07; wu:fi14e05

more info or order :

Invitrogen product webpage