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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1), eBioscience
catalog :
14-3249-82
quantity :
100 µg
price :
US 288.00
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
DECMA-1
reactivity :
human, mouse, dogs
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 65
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; 1:500; loading ...; fig s1c
Dumortier J, Le Verge Serandour M, Tortorelli A, Mielke A, de Plater L, Turlier H, et al. Hydraulic fracturing and active coarsening position the lumen of the mouse blastocyst. Science. 2019;365:465-468 pubmed publisher
  • immunohistochemistry; mouse; 1:800; loading ...; fig 6a
Cetera M, Leybova L, Woo F, Deans M, Devenport D. Planar cell polarity-dependent and independent functions in the emergence of tissue-scale hair follicle patterns. Dev Biol. 2017;428:188-203 pubmed publisher
  • immunohistochemistry; mouse; 1:700; loading ...; fig s6c
Scheele C, Hannezo E, Muraro M, Zomer A, Langedijk N, van Oudenaarden A, et al. Identity and dynamics of mammary stem cells during branching morphogenesis. Nature. 2017;542:313-317 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:800; loading ...; fig 6e
Wuidart A, Ousset M, Rulands S, Simons B, Van Keymeulen A, Blanpain C. Quantitative lineage tracing strategies to resolve multipotency in tissue-specific stem cells. Genes Dev. 2016;30:1261-77 pubmed publisher
  • flow cytometry; mouse; 1:150
Ludigs K, Jandus C, Utzschneider D, Staehli F, Bessoles S, Dang A, et al. NLRC5 shields T lymphocytes from NK-cell-mediated elimination under inflammatory conditions. Nat Commun. 2016;7:10554 pubmed publisher
  • flow cytometry; mouse; fig 6
Xia H, Ren X, Bolte C, Ustiyan V, Zhang Y, Shah T, et al. Foxm1 regulates resolution of hyperoxic lung injury in newborns. Am J Respir Cell Mol Biol. 2015;52:611-21 pubmed publisher
Zhou H, Yun X, Shu Y, Xu K. Aspirin increases the efficacy of gemcitabine in pancreatic cancer by modulating the PI3K/AKT/mTOR signaling pathway and reversing epithelial‑mesenchymal transition. Oncol Lett. 2023;25:101 pubmed publisher
Olde Hanhof C, Dilmen E, Yousef Yengej F, Latta F, Ammerlaan C, Schreurs J, et al. Differentiated mouse kidney tubuloids as a novel in vitro model to study collecting duct physiology. Front Cell Dev Biol. 2023;11:1086823 pubmed publisher
Basta L, Sil P, Jones R, Little K, Hayward Lara G, Devenport D. Celsr1 and Celsr2 exhibit distinct adhesive interactions and contributions to planar cell polarity. Front Cell Dev Biol. 2022;10:1064907 pubmed publisher
Meena A, Shukla P, Bell B, Giorgianni F, Caires R, Fernández Peña C, et al. TRPV6 channel mediates alcohol-induced gut barrier dysfunction and systemic response. Cell Rep. 2022;39:110937 pubmed publisher
Zisis T, Brückner D, Brandstätter T, Siow W, D Alessandro J, Vollmar A, et al. Disentangling cadherin-mediated cell-cell interactions in collective cancer cell migration. Biophys J. 2022;121:44-60 pubmed publisher
Choi D, Gonzalez Z, Ho S, Bermudez A, Lin N. Cell-cell adhesion impacts epithelia response to substrate stiffness: Morphology and gene expression. Biophys J. 2022;121:336-346 pubmed publisher
Li L, Hu Y, Chen D, Zhu J, Bao W, Xu X, et al. CMTM5 inhibits the development of prostate cancer via the EGFR/PI3K/AKT signaling pathway. Mol Med Rep. 2022;25: pubmed publisher
Kurhe Y, Caputo M, Cansby E, Xia Y, Kumari S, Anand S, et al. Antagonizing STK25 Signaling Suppresses the Development of Hepatocellular Carcinoma Through Targeting Metabolic, Inflammatory, and Pro-Oncogenic Pathways. Cell Mol Gastroenterol Hepatol. 2022;13:405-423 pubmed publisher
He Z, Lei S, Liang F, Tan L, Zhang W, Xie L, et al. Low-Dose Albendazole Inhibits Epithelial-Mesenchymal Transition of Melanoma Cells by Enhancing Phosphorylated GSK-3β/Tyr216 Accumulation. J Oncol. 2021;2021:4475192 pubmed publisher
Hsu S, Chou L, Hong C, Chang M, Tsai C, Tian Y, et al. Crosstalk between E-Cadherin/β-Catenin and NF-κB Signaling Pathways: The Regulation of Host-Pathogen Interaction during Leptospirosis. Int J Mol Sci. 2021;22: pubmed publisher
Troitskaya O, Novak D, Nushtaeva A, Savinkova M, Varlamov M, Ermakov M, et al. EGFR Transgene Stimulates Spontaneous Formation of MCF7 Breast Cancer Cells Spheroids with Partly Loss of HER3 Receptor. Int J Mol Sci. 2021;22: pubmed publisher
Oyama N, Kawaguchi M, Itaka K, Kawakami S. Efficient Messenger RNA Delivery to the Kidney Using Renal Pelvis Injection in Mice. Pharmaceutics. 2021;13: pubmed publisher
Begum H, Mariano C, Zhou H, Shen K. E-Cadherin Regulates Mitochondrial Membrane Potential in Cancer Cells. Cancers (Basel). 2021;13: pubmed publisher
Fujioka N, Kitabatake M, Ouji Sageshima N, Ibaraki T, Kumamoto M, Fujita Y, et al. Human Adipose-Derived Mesenchymal Stem Cells Ameliorate Elastase-Induced Emphysema in Mice by Mesenchymal-Epithelial Transition. Int J Chron Obstruct Pulmon Dis. 2021;16:2783-2793 pubmed publisher
Billings S, Myers N, Quiruz L, Cheng A. Opposing effects of Wnt/β-catenin signaling on epithelial and mesenchymal cell fate in the developing cochlea. Development. 2021;148: pubmed publisher
Zeng X, Liao H, Wang F. MicroRNA-384 inhibits nasopharyngeal carcinoma growth and metastasis via binding to Smad5 and suppressing the Wnt/β-catenin axis. Cytotechnology. 2021;73:203-215 pubmed publisher
Schliffka M, Tortorelli A, xd6 zg xfc xe7 x, de Plater L, Polzer O, Pelzer D, et al. Multiscale analysis of single and double maternal-zygotic Myh9 and Myh10 mutants during mouse preimplantation development. elife. 2021;10: pubmed publisher
Kim J, Mahiddine F, Kim G. Leptin Modulates the Metastasis of Canine Inflammatory Mammary Adenocarcinoma Cells Through Downregulation of Lysosomal Protective Protein Cathepsin A (CTSA). Int J Mol Sci. 2020;21: pubmed publisher
Duncan Lowey J, Wiscovitch A, Wood T, Goldberg M, Russo B. Shigella flexneri Disruption of Cellular Tension Promotes Intercellular Spread. Cell Rep. 2020;33:108409 pubmed publisher
Fomicheva M, Macara I. Genome-wide CRISPR screen identifies noncanonical NF-κB signaling as a regulator of density-dependent proliferation. elife. 2020;9: pubmed publisher
Centonze A, Lin S, Tika E, Sifrim A, Fioramonti M, Malfait M, et al. Heterotypic cell-cell communication regulates glandular stem cell multipotency. Nature. 2020;584:608-613 pubmed publisher
Aragona M, Sifrim A, Malfait M, Song Y, Van Herck J, Dekoninck S, et al. Mechanisms of stretch-mediated skin expansion at single-cell resolution. Nature. 2020;584:268-273 pubmed publisher
DIXON E, Maxim D, Halperin Kuhns V, Lane Harris A, Outeda P, Ewald A, et al. GDNF drives rapid tubule morphogenesis in a novel 3D in vitro model for ADPKD. J Cell Sci. 2020;133: pubmed publisher
Vila Ellis L, Cain M, Hutchison V, Flodby P, Crandall E, Borok Z, et al. Epithelial Vegfa Specifies a Distinct Endothelial Population in the Mouse Lung. Dev Cell. 2020;52:617-630.e6 pubmed publisher
Bornes L, van Scheppingen R, Beerling E, Schelfhorst T, Ellenbroek S, Seinstra D, et al. Fsp1-Mediated Lineage Tracing Fails to Detect the Majority of Disseminating Cells Undergoing EMT. Cell Rep. 2019;29:2565-2569.e3 pubmed publisher
Tran K, Dillingham C, Sridharan R. Coordinated removal of repressive epigenetic modifications during induced reversal of cell identity. EMBO J. 2019;38:e101681 pubmed publisher
Malik V, Glaser L, Zimmer D, Velychko S, Weng M, Holzner M, et al. Pluripotency reprogramming by competent and incompetent POU factors uncovers temporal dependency for Oct4 and Sox2. Nat Commun. 2019;10:3477 pubmed publisher
Tran K, Pietrzak S, Zaidan N, Siahpirani A, McCalla S, Zhou A, et al. Defining Reprogramming Checkpoints from Single-Cell Analyses of Induced Pluripotency. Cell Rep. 2019;27:1726-1741.e5 pubmed publisher
Flood B, Manils J, Nulty C, Flis E, Kenealy S, Barber G, et al. Caspase-11 regulates the tumour suppressor function of STAT1 in a murine model of colitis-associated carcinogenesis. Oncogene. 2019;38:2658-2674 pubmed publisher
Wuidart A, Sifrim A, Fioramonti M, Matsumura S, Brisebarre A, Brown D, et al. Early lineage segregation of multipotent embryonic mammary gland progenitors. Nat Cell Biol. 2018;20:666-676 pubmed publisher
Ostrin E, Little D, Gerner Mauro K, Sumner E, Ríos Corzo R, Ambrosio E, et al. β-Catenin maintains lung epithelial progenitors after lung specification. Development. 2018;145: pubmed publisher
Chua C, Epsi N, Leung E, Xuan S, Lei M, Li B, et al. Differential requirements of androgen receptor in luminal progenitors during prostate regeneration and tumor initiation. elife. 2018;7: pubmed publisher
Heck B, Devenport D. Trans-endocytosis of Planar Cell Polarity Complexes during Cell Division. Curr Biol. 2017;27:3725-3733.e4 pubmed publisher
Ruetz T, Pfisterer U, Di Stefano B, Ashmore J, Beniazza M, Tian T, et al. Constitutively Active SMAD2/3 Are Broad-Scope Potentiators of Transcription-Factor-Mediated Cellular Reprogramming. Cell Stem Cell. 2017;21:791-805.e9 pubmed publisher
Hannezo E, Scheele C, Moad M, Drogo N, Heer R, Sampogna R, et al. A Unifying Theory of Branching Morphogenesis. Cell. 2017;171:242-255.e27 pubmed publisher
Lyons Cohen M, Thomas S, Cook D, Nakano H. Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells. J Vis Exp. 2017;: pubmed publisher
Meinicke H, Bremser A, Brack M, Schrenk K, Pircher H, Izcue A. KLRG1 impairs regulatory T-cell competitive fitness in the gut. Immunology. 2017;152:65-73 pubmed publisher
Nakano H, Lyons Cohen M, Whitehead G, Nakano K, Cook D. Distinct functions of CXCR4, CCR2, and CX3CR1 direct dendritic cell precursors from the bone marrow to the lung. J Leukoc Biol. 2017;101:1143-1153 pubmed publisher
Latil M, Nassar D, Beck B, Boumahdi S, Wang L, Brisebarre A, et al. Cell-Type-Specific Chromatin States Differentially Prime Squamous Cell Carcinoma Tumor-Initiating Cells for Epithelial to Mesenchymal Transition. Cell Stem Cell. 2017;20:191-204.e5 pubmed publisher
Oren B, Urosevic J, Mertens C, Mora J, Guiu M, Gomis R, et al. Tumour stroma-derived lipocalin-2 promotes breast cancer metastasis. J Pathol. 2016;239:274-85 pubmed publisher
Jackson S, Olufs Z, Tran K, Zaidan N, Sridharan R. Alternative Routes to Induced Pluripotent Stem Cells Revealed by Reprogramming of the Neural Lineage. Stem Cell Reports. 2016;6:302-11 pubmed publisher
Zheng X, Carstens J, Kim J, Scheible M, Kaye J, Sugimoto H, et al. Epithelial-to-mesenchymal transition is dispensable for metastasis but induces chemoresistance in pancreatic cancer. Nature. 2015;527:525-530 pubmed publisher
Chantzoura E, Skylaki S, Menendez S, Kim S, Johnsson A, Linnarsson S, et al. Reprogramming Roadblocks Are System Dependent. Stem Cell Reports. 2015;5:350-64 pubmed publisher
Lovisa S, LeBleu V, Tampe B, Sugimoto H, Vadnagara K, Carstens J, et al. Epithelial-to-mesenchymal transition induces cell cycle arrest and parenchymal damage in renal fibrosis. Nat Med. 2015;21:998-1009 pubmed publisher
Liang S, Marti T, Dorn P, Froment L, Hall S, Berezowska S, et al. Blocking the epithelial-to-mesenchymal transition pathway abrogates resistance to anti-folate chemotherapy in lung cancer. Cell Death Dis. 2015;6:e1824 pubmed publisher
Steinert E, Schenkel J, Fraser K, Beura L, Manlove L, Igyártó B, et al. Quantifying Memory CD8 T Cells Reveals Regionalization of Immunosurveillance. Cell. 2015;161:737-49 pubmed publisher
Wang S, Wang B, Pan N, Fu L, Wang C, Song G, et al. Differentiation of human induced pluripotent stem cells to mature functional Purkinje neurons. Sci Rep. 2015;5:9232 pubmed publisher
Chua C, Shibata M, Lei M, Toivanen R, Barlow L, Bergren S, et al. Single luminal epithelial progenitors can generate prostate organoids in culture. Nat Cell Biol. 2014;16:951-61, 1-4 pubmed publisher
Matsuo K, Ishii Y, Kawai Y, Saiba Y, Quan Y, Kamiyama F, et al. Analysis of transcutaneous antigenic protein delivery by a hydrogel patch formulation. J Pharm Sci. 2013;102:1936-1947 pubmed publisher
Kobayashi A, Donaldson D, Kanaya T, Fukuda S, Baillie J, Freeman T, et al. Identification of novel genes selectively expressed in the follicle-associated epithelium from the meta-analysis of transcriptomics data from multiple mouse cell and tissue populations. DNA Res. 2012;19:407-22 pubmed publisher
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Spencer H, Eastham A, Merry C, Southgate T, Perez Campo F, Soncin F, et al. E-cadherin inhibits cell surface localization of the pro-migratory 5T4 oncofetal antigen in mouse embryonic stem cells. Mol Biol Cell. 2007;18:2838-51 pubmed
Liu W, Nelson C, Pirone D, Chen C. E-cadherin engagement stimulates proliferation via Rac1. J Cell Biol. 2006;173:431-41 pubmed
Chattopadhyay N, Wang Z, Ashman L, Brady Kalnay S, Kreidberg J. alpha3beta1 integrin-CD151, a component of the cadherin-catenin complex, regulates PTPmu expression and cell-cell adhesion. J Cell Biol. 2003;163:1351-62 pubmed
Nakagawa M, Fukata M, Yamaga M, Itoh N, Kaibuchi K. Recruitment and activation of Rac1 by the formation of E-cadherin-mediated cell-cell adhesion sites. J Cell Sci. 2001;114:1829-38 pubmed
Lee M, Sharrow S, Farr A, Singer A, Udey M. Expression of the homotypic adhesion molecule E-cadherin by immature murine thymocytes and thymic epithelial cells. J Immunol. 1994;152:5653-9 pubmed
Tang A, Eller M, Hara M, Yaar M, Hirohashi S, Gilchrest B. E-cadherin is the major mediator of human melanocyte adhesion to keratinocytes in vitro. J Cell Sci. 1994;107 ( Pt 4):983-92 pubmed
Vestweber D, Kemler R. Identification of a putative cell adhesion domain of uvomorulin. EMBO J. 1985;4:3393-8 pubmed
product information
Product Type :
Antibody
Product Name :
CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1), eBioscience
Catalog # :
14-3249-82
Quantity :
100 µg
Price :
US 288.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Canine, Human, Mouse
Applications :
ELISA: Assay-Dependent, Flow Cytometry: Assay-Dependent, Functional Assay: Assay-Dependent, Immunocytochemistry: 5 µg/mL, Immunohistochemistry (Frozen): Assay-Dependent, Immunoprecipitation: Assay-Dependent, Western Blot: 2 µg/mL
Species :
Canine, Human, Mouse
Clone :
DECMA-1
Isotype :
IgG1, kappa
Storage :
4° C
Description :
E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.
Format :
Liquid
Applications w/Dilutions :
ELISA: Assay-Dependent, Flow Cytometry: Assay-Dependent, Functional Assay: Assay-Dependent, Immunocytochemistry: 5 µg/mL, Immunohistochemistry (Frozen): Assay-Dependent, Immunoprecipitation: Assay-Dependent, Western Blot: 2 µg/mL
Aliases :
AA960649; ARC-1; cadherin 1; cadherin 1, E-cadherin (epithelial); cadherin 1, epithelial; cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin e; cadherin-1; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; CDH1; CDHE; cell adhesion molecule; cell-CAM 120/80; ECAD; E-cad; E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3; Ecadherin; E-cadherin; E-cadherin 1; E-cadherin precursor; Epithelial cadherin; hab; half baked; LCAM; L-CAM; Um; UVO; Uvomorulin
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA