CD163 Monoclonal Antibody (eBioGHI/61 (GHI/61)), eBioscience | Invitrogen 14-1639-37 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD163 Monoclonal Antibody (eBioGHI/61 (GHI/61)), eBioscience

catalog :

14-1639-37

quantity :

2 mg

price :

US 1452

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

eBioGHI/61 (GHI/61)

reactivity :

human, mouse

application :

western blot, flow cytometry

more info or order :

Invitrogen product webpage

citations: 28

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 6e	Zhao Y, Sun J, Li Y, Zhou X, Zhai W, Wu Y, et al. Tryptophan 2,3-dioxygenase 2 controls M2 macrophages polarization to promote esophageal squamous cell carcinoma progression via AKT/GSK3β/IL-8 signaling pathway. Acta Pharm Sin B. 2021;11:2835-2849 pubmed publisher
	Mou J, Zheng W, Wei D, Li D, Fan R, Tang Q. CD200-CD200R affects cisplatin and paclitaxel sensitivity by regulating cathepsin K-mediated p65 NF-κB signaling in cervical cancer. Heliyon. 2023;9:e19220 pubmed publisher
	Wang W, Li X, Qin X, Miao Y, Zhang Y, Li S, et al. Germline Neurofibromin 1 mutation enhances the anti-tumour immune response and decreases juvenile myelomonocytic leukaemia tumourigenicity. Br J Haematol. 2023;202:328-343 pubmed publisher
	Thaweerattanasinp T, Wanitchang A, Saenboonrueng J, Srisutthisamphan K, Wanasen N, Sungsuwan S, et al. SARS-CoV-2 Delta (B.1.617.2) variant replicates and induces syncytia formation in human induced pluripotent stem cell-derived macrophages. Peerj. 2023;11:e14918 pubmed publisher
	Fang J, Ou Q, Wu B, Li S, Wu M, Qiu J, et al. TcpC Inhibits M1 but Promotes M2 Macrophage Polarization via Regulation of the MAPK/NF-κB and Akt/STAT6 Pathways in Urinary Tract Infection. Cells. 2022;11: pubmed publisher
	Zhao G, Ding L, Yu H, Wang W, Wang H, Hu Y, et al. M2-like tumor-associated macrophages transmit exosomal miR-27b-3p and maintain glioblastoma stem-like cell properties. Cell Death Discov. 2022;8:350 pubmed publisher
	Zhang H, Wang Y, Zhao Y, Liu T, Wang Z, Zhang N, et al. PTX3 mediates the infiltration, migration, and inflammation-resolving-polarization of macrophages in glioblastoma. CNS Neurosci Ther. 2022;28:1748-1766 pubmed publisher
	Yan X, He Y, Yang S, Zeng T, Hua Y, Bao S, et al. A positive feedback loop: RAD18-YAP-TGF-β between triple-negative breast cancer and macrophages regulates cancer stemness and progression. Cell Death Discov. 2022;8:196 pubmed publisher
	Jiang H, Zhou L, Shen N, Ning X, Wu D, Jiang K, et al. M1 macrophage-derived exosomes and their key molecule lncRNA HOTTIP suppress head and neck squamous cell carcinoma progression by upregulating the TLR5/NF-κB pathway. Cell Death Dis. 2022;13:183 pubmed publisher
	Higham A, Baker J, Jackson N, Shah R, Lea S, Singh D. Dysregulation of the CD163-Haptoglobin Axis in the Airways of COPD Patients. Cells. 2021;11: pubmed publisher
	Janosz E, Hetzel M, Spielmann H, Tumpara S, Rossdam C, Schwabbauer M, et al. Pulmonary transplantation of alpha-1 antitrypsin (AAT)-transgenic macrophages provides a source of functional human AAT in vivo. Gene Ther. 2021;28:477-493 pubmed publisher
	Huang R, Wang S, Wang N, Zheng Y, Zhou J, Yang B, et al. CCL5 derived from tumor-associated macrophages promotes prostate cancer stem cells and metastasis via activating β-catenin/STAT3 signaling. Cell Death Dis. 2020;11:234 pubmed publisher
	Taleska Stupica G, Sostaric M, Bozhinovska M, Rupert L, Bosnić Z, Jerin A, et al. Extracorporeal Hemadsorption versus Glucocorticoids during Cardiopulmonary Bypass: A Prospective, Randomized, Controlled Trial. Cardiovasc Ther. 2020;2020:7834173 pubmed publisher
	Haake K, Neehus A, Buchegger T, Kühnel M, Blank P, Philipp F, et al. Patient iPSC-Derived Macrophages to Study Inborn Errors of the IFN-γ Responsive Pathway. Cells. 2020;9: pubmed publisher
	Wilcz Villega E, Carter E, Ironside A, Xu R, Mataloni I, Holdsworth J, et al. Macrophages induce malignant traits in mammary epithelium via IKKε/TBK1 kinases and the serine biosynthesis pathway. EMBO Mol Med. 2020;12:e10491 pubmed publisher
	Shi S, Lee E, Lin Y, Chen L, Zheng H, He X, et al. Recruitment of monocytes and epigenetic silencing of intratumoral CYP7B1 primarily contribute to the accumulation of 27-hydroxycholesterol in breast cancer. Am J Cancer Res. 2019;9:2194-2208 pubmed
	Georgouli M, Herraiz C, Crosas Molist E, Fanshawe B, Maiques O, Perdrix A, et al. Regional Activation of Myosin II in Cancer Cells Drives Tumor Progression via a Secretory Cross-Talk with the Immune Microenvironment. Cell. 2019;176:757-774.e23 pubmed publisher
	Zhao X, Tang X, Guo N, An Y, Chen X, Shi C, et al. Biochanin a Enhances the Defense Against Salmonella enterica Infection Through AMPK/ULK1/mTOR-Mediated Autophagy and Extracellular Traps and Reversing SPI-1-Dependent Macrophage (MΦ) M2 Polarization. Front Cell Infect Microbiol. 2018;8:318 pubmed publisher
	Narasimhan P, Akabas L, Tariq S, Huda N, Bennuru S, Sabzevari H, et al. Similarities and differences between helminth parasites and cancer cell lines in shaping human monocytes: Insights into parallel mechanisms of immune evasion. PLoS Negl Trop Dis. 2018;12:e0006404 pubmed publisher
	Wu H, Xu X, Li J, Gong J, Li M. TIM‑4 blockade of KCs combined with exogenous TGF‑β injection helps to reverse acute rejection and prolong the survival rate of mice receiving liver allografts. Int J Mol Med. 2018;42:346-358 pubmed publisher
	Zhu Z, Ding J, Ma Z, Iwashina T, Tredget E. Alternatively activated macrophages derived from THP-1 cells promote the fibrogenic activities of human dermal fibroblasts. Wound Repair Regen. 2017;25:377-388 pubmed publisher
	Wonderlich E, Swan Z, Bissel S, Hartman A, Carney J, O Malley K, et al. Widespread Virus Replication in Alveoli Drives Acute Respiratory Distress Syndrome in Aerosolized H5N1 Influenza Infection of Macaques. J Immunol. 2017;198:1616-1626 pubmed publisher
	Garcia Mesa Y, Jay T, Checkley M, Luttge B, Dobrowolski C, Valadkhan S, et al. Immortalization of primary microglia: a new platform to study HIV regulation in the central nervous system. J Neurovirol. 2017;23:47-66 pubmed publisher
	Zhong Y, Yi C. MicroRNA-720 suppresses M2 macrophage polarization by targeting GATA3. Biosci Rep. 2016;36: pubmed publisher
	Park C, Arthos J, Cicala C, Kehrl J. The HIV-1 envelope protein gp120 is captured and displayed for B cell recognition by SIGN-R1(+) lymph node macrophages. elife. 2015;4: pubmed publisher
	Mattila J, Ojo O, Kepka Lenhart D, Marino S, Kim J, Eum S, et al. Microenvironments in tuberculous granulomas are delineated by distinct populations of macrophage subsets and expression of nitric oxide synthase and arginase isoforms. J Immunol. 2013;191:773-84 pubmed publisher
	Banerjee S, Lin C, Skinner K, Schiffhauer L, Peacock J, Hicks D, et al. Heat shock protein 27 differentiates tolerogenic macrophages that may support human breast cancer progression. Cancer Res. 2011;71:318-27 pubmed publisher
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product information

Product Type :

Antibody

Product Name :

CD163 Monoclonal Antibody (eBioGHI/61 (GHI/61)), eBioscience

Catalog # :

14-1639-37

Quantity :

2 mg

Price :

US 1452

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: 0.5 µg/test, Western Blot: Assay-Dependent

Species :

Human

Clone :

eBioGHI/61 (GHI/61)

Isotype :

IgG1, kappa

Storage :

4° C

Description :

CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein, a member of the scavenger receptor cysteine-rich superfamily, and a receptor for the hemoglobin-haptoglobin complex. CD163 protects tissues from free hemoglobin-mediated oxidative damage, and may play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. Specifically, CD163 is present on all circulating monocytes and most tissue macrophages except those found in the mantle zone and germinal centers of lymphoid follicles, interdigitating reticulum cells and Langerhan's cells. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant. CD163 binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner, and exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP1S phenotype. Further, CD163 also induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.5 µg/test, Western Blot: Assay-Dependent

Aliases :

CD163; CD163 antigen; CD163 molecule; CD163v2; CD163v3; Hemoglobin scavenger receptor; M130; macrophage-associated antigen; MM130; putative CD163 antigen; SCARI1; Scavenger receptor cysteine-rich type 1 protein M130; sCD163; Soluble CD163; Soluble sCD163

more info or order :

Invitrogen product webpage