CD117 (c-Kit) Monoclonal Antibody (YB5.B8), eBioscience | Invitrogen 14-1179-82 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD117 (c-Kit) Monoclonal Antibody (YB5.B8), eBioscience

catalog :

14-1179-82

quantity :

100 µg

price :

US 237.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

YB5.B8

reactivity :

human

application :

immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; fig 6	Arlehamn C, Seumois G, Gerasimova A, Huang C, Fu Z, Yue X, et al. Transcriptional profile of tuberculosis antigen-specific T cells reveals novel multifunctional features. J Immunol. 2014;193:2931-40 pubmed publisher
	Kim K, Kim B, Won J, Min H, Lee K, Lee S, et al. Regulation of osteoclastogenesis by mast cell in rheumatoid arthritis. Arthritis Res Ther. 2021;23:124 pubmed publisher
	McKenney A, Lau A, Somasundara A, Spitzer B, Intlekofer A, Ahn J, et al. JAK2/IDH-mutant-driven myeloproliferative neoplasm is sensitive to combined targeted inhibition. J Clin Invest. 2018;128:789-804 pubmed publisher
	Igarashi A, Ebihara Y, Kumagai T, Hirai H, Nagata K, Tsuji K. Mast cells derived from human induced pluripotent stem cells are useful for allergen tests. Allergol Int. 2018;67:234-242 pubmed publisher
	Hatzistergos K, Saur D, Seidler B, Balkan W, Breton M, Valasaki K, et al. Stimulatory Effects of Mesenchymal Stem Cells on cKit+ Cardiac Stem Cells Are Mediated by SDF1/CXCR4 and SCF/cKit Signaling Pathways. Circ Res. 2016;119:921-30 pubmed publisher
	Oikawa T, Wauthier E, Dinh T, Selitsky S, Reyna Neyra A, Carpino G, et al. Model of fibrolamellar hepatocellular carcinomas reveals striking enrichment in cancer stem cells. Nat Commun. 2015;6:8070 pubmed publisher
	Sugawa F, AraÃºzo Bravo M, Yoon J, Kim K, Aramaki S, Wu G, et al. Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile. EMBO J. 2015;34:1009-24 pubmed publisher
	Indumathi S, Harikrishnan R, Rajkumar J, Dhanasekaran M. Immunophenotypic comparison of heterogenous non-sorted versus sorted mononuclear cells from human umbilical cord blood: a novel cell enrichment approach. Cytotechnology. 2015;67:107-14 pubmed publisher
	Acharya S, Kaplan R, Macdonald D, Fabiyi O, DiMichele D, Lyden D. Neoangiogenesis contributes to the development of hemophilic synovitis. Blood. 2011;117:2484-93 pubmed publisher
	Leong K, Wang B, Johnson L, Gao W. Generation of a prostate from a single adult stem cell. Nature. 2008;456:804-8 pubmed publisher
	Okayama Y, Hunt T, Kassel O, Ashman L, Church M. Assessment of the anti-c-kit monoclonal antibody YB5.B8 in affinity magnetic enrichment of human lung mast cells. J Immunol Methods. 1994;169:153-61 pubmed
	Ashman L, Buhring H, Aylett G, Broudy V, Muller C. Epitope mapping and functional studies with three monoclonal antibodies to the c-kit receptor tyrosine kinase, YB5.B8, 17F11, and SR-1. J Cell Physiol. 1994;158:545-54 pubmed
	Lerner N, Nocka K, Cole S, Qiu F, Strife A, Ashman L, et al. Monoclonal antibody YB5.B8 identifies the human c-kit protein product. Blood. 1991;77:1876-83 pubmed

product information

Product Type :

Antibody

Product Name :

CD117 (c-Kit) Monoclonal Antibody (YB5.B8), eBioscience

Catalog # :

14-1179-82

Quantity :

100 µg

Price :

US 237.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: 1 µg/test, Immunohistochemistry (Frozen): Assay-Dependent, Immunoprecipitation: Assay-Dependent

Species :

Human

Clone :

YB5.B8

Isotype :

IgG1, kappa

Storage :

4° C

Description :

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1 µg/test, Immunohistochemistry (Frozen): Assay-Dependent, Immunoprecipitation: Assay-Dependent

Aliases :

belly-spot; Bs; CD117; ckit; C-Kit; c-KIT gene1; c-kit protein; c-kit proto-oncogene protein; c-kit receptor; c-kit receptor tyrosine kinase; dominant spotting; Dominant white spotting; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; KIT; kit oncogene; KIT proto-oncogene receptor tyrosine kinase; KIT proto-oncogene, receptor tyrosine kinase; mast cell growth factor receptor; mast/stem cell growth factor receptor; mast/stem cell growth factor receptor Kit; MGF; p145 c-kit; PBT; Piebald trait protein; protein kinase; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; receptor tyrosine kinase c-kit; receptor tyrosine kinase type III; SCFR; SCO1; SCO5; Sl; soluble KIT variant 1; SOW3; spotted sterile male; Ssm; Steel Factor Receptor; Tr-kit; tyrosine kinase receptor; tyrosine kinase receptor c-KIT; tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein; W

more info or order :

Invitrogen product webpage