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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), eBioscience
catalog :
14-1079-80
quantity :
25 µg
price :
US 76.50
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
eBioH4A3
reactivity :
human, rhesus macaque
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 60
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 2a
Pugh J, Nemat Gorgani N, Norman P, Guethlein L, Parham P. Human NK Cells Downregulate Zap70 and Syk in Response to Prolonged Activation or DNA Damage. J Immunol. 2018;200:1146-1158 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 3f
Burr M, Sparbier C, Chan Y, Williamson J, Woods K, Beavis P, et al. CMTM6 maintains the expression of PD-L1 and regulates anti-tumour immunity. Nature. 2017;549:101-105 pubmed publisher
  • flow cytometry; human; fig s1b
Cuff A, Robertson F, Stegmann K, Pallett L, Maini M, Davidson B, et al. Eomeshi NK Cells in Human Liver Are Long-Lived and Do Not Recirculate but Can Be Replenished from the Circulation. J Immunol. 2016;197:4283-4291 pubmed
  • flow cytometry; rhesus macaque; fig 4d
Hu X, Valentin A, Dayton F, Kulkarni V, Alicea C, Rosati M, et al. DNA Prime-Boost Vaccine Regimen To Increase Breadth, Magnitude, and Cytotoxicity of the Cellular Immune Responses to Subdominant Gag Epitopes of Simian Immunodeficiency Virus and HIV. J Immunol. 2016;197:3999-4013 pubmed
  • flow cytometry; human; loading ...; fig 1d
Wahid R, Fresnay S, Levine M, Sztein M. Cross-reactive multifunctional CD4+ T cell responses against Salmonella enterica serovars Typhi, Paratyphi A and Paratyphi B in humans following immunization with live oral typhoid vaccine Ty21a. Clin Immunol. 2016;173:87-95 pubmed publisher
  • flow cytometry; rhesus macaque; loading ...; fig 5a
Vargas Inchaustegui D, Ying O, Demberg T, Robert Guroff M. Evaluation of Functional NK Cell Responses in Vaccinated and SIV-Infected Rhesus Macaques. Front Immunol. 2016;7:340 pubmed publisher
  • flow cytometry; human; loading ...; fig 4d
Demers K, Makedonas G, Buggert M, Eller M, Ratcliffe S, Goonetilleke N, et al. Temporal Dynamics of CD8+ T Cell Effector Responses during Primary HIV Infection. PLoS Pathog. 2016;12:e1005805 pubmed publisher
  • flow cytometry; human; fig 4
Yin W, Tong S, Zhang Q, Shao J, Liu Q, Peng H, et al. Functional dichotomy of Vδ2 γδ T cells in chronic hepatitis C virus infections: role in cytotoxicity but not for IFN-γ production. Sci Rep. 2016;6:26296 pubmed publisher
  • flow cytometry; human; loading ...; fig 4a
Dimitrova M, Zenarruzabeitia O, Borrego F, Simhadri V. CD300c is uniquely expressed on CD56 bright Natural Killer Cells and differs from CD300a upon ligand recognition. Sci Rep. 2016;6:23942 pubmed publisher
  • flow cytometry; human; fig s3
Tsai C, Liong K, Gunalan M, Li N, Lim D, Fisher D, et al. Type I IFNs and IL-18 regulate the antiviral response of primary human γδ T cells against dendritic cells infected with Dengue virus. J Immunol. 2015;194:3890-900 pubmed publisher
  • flow cytometry; human
Lim D, Yawata N, Selva K, Li N, Tsai C, Yeong L, et al. The combination of type I IFN, TNF-α, and cell surface receptor engagement with dendritic cells enables NK cells to overcome immune evasion by dengue virus. J Immunol. 2014;193:5065-75 pubmed publisher
Ding L, Chen X, Zhang W, Dai X, Guo H, Pan X, et al. Canagliflozin primes antitumor immunity by triggering PD-L1 degradation in endocytic recycling. J Clin Invest. 2023;133: pubmed publisher
Molero Valenzuela A, Fontova P, Alonso Carrillo D, Carreira Barral I, Torres A, Garc xed a Valverde M, et al. A Novel Late-Stage Autophagy Inhibitor That Efficiently Targets Lysosomes Inducing Potent Cytotoxic and Sensitizing Effects in Lung Cancer. Cancers (Basel). 2022;14: pubmed publisher
von Schoenfeld A, Bronsert P, Poc M, Fuller A, Filby A, Kraft S, et al. Multiple Immunostainings with Different Epitope Retrievals-The FOLGAS Protocol. Int J Mol Sci. 2021;23: pubmed publisher
Betriu N, Andreeva A, Semino C. Erlotinib Promotes Ligand-Induced EGFR Degradation in 3D but Not 2D Cultures of Pancreatic Ductal Adenocarcinoma Cells. Cancers (Basel). 2021;13: pubmed publisher
Biadglegne F, Rademacher P, De Sulbaran Y, K xf6 nig B, Rodloff A, Zedler U, et al. Exosomes in serum‑free cultures of THP‑1 macrophages infected with Mycobacterium tuberculosis. Mol Med Rep. 2021;24: pubmed publisher
Silva J, Qi X, Grant M, Boulton M. Spatial and temporal VEGF receptor intracellular trafficking in microvascular and macrovascular endothelial cells. Sci Rep. 2021;11:17400 pubmed publisher
Ressnerova A, Novotný F, Michalkova H, Pumera M, Adam V, Heger Z. Efficient Protein Transfection by Swarms of Chemically Powered Plasmonic Virus-Sized Nanorobots. ACS Nano. 2021;15:12899-12910 pubmed publisher
Tokarchuk I, Janser F, Schläfli A, Pinto M, Humbert M, Niklaus N, et al. Increased LAMP2A levels correlate with a shorter disease-free survival of HER2 negative breast cancer patients and increased breast cancer cell viability. Biochem Biophys Res Commun. 2021;569:47-53 pubmed publisher
Zheng G, Guo Z, Li W, Xi W, Zuo B, Zhang R, et al. Interaction between HLA-G and NK cell receptor KIR2DL4 orchestrates HER2-positive breast cancer resistance to trastuzumab. Signal Transduct Target Ther. 2021;6:236 pubmed publisher
Cejas R, Tamaño Blanco M, Blanco J. Analysis of the intracellular traffic of IgG in the context of Down syndrome (trisomy 21). Sci Rep. 2021;11:10981 pubmed publisher
Humbert M, Seiler K, Mosimann S, Rentsch V, Sharma K, Pandey A, et al. Reducing FASN expression sensitizes acute myeloid leukemia cells to differentiation therapy. Cell Death Differ. 2021;28:2465-2481 pubmed publisher
Hope J, Bersi M, Dombroski J, Clinch A, Pereles R, Merryman W, et al. Circulating prostate cancer cells have differential resistance to fluid shear stress-induced cell death. J Cell Sci. 2021;134: pubmed publisher
Skubalova Z, Rex S, Sukupova M, Zahalka M, Skladal P, Pribyl J, et al. Passive Diffusion vs Active pH-Dependent Encapsulation of Tyrosine Kinase Inhibitors Vandetanib and Lenvatinib into Folate-Targeted Ferritin Delivery System. Int J Nanomedicine. 2021;16:1-14 pubmed publisher
Menasche B, Davis E, Wang S, Ouyang Y, Li S, Yu H, et al. PBRM1 and the glycosylphosphatidylinositol biosynthetic pathway promote tumor killing mediated by MHC-unrestricted cytotoxic lymphocytes. Sci Adv. 2020;6: pubmed publisher
Sant S, Quiñones Parra S, Koutsakos M, Grant E, Loudovaris T, Mannering S, et al. HLA-B*27:05 alters immunodominance hierarchy of universal influenza-specific CD8+ T cells. PLoS Pathog. 2020;16:e1008714 pubmed publisher
Stunnenberg M, van Pul L, Sprokholt J, van Dort K, Gringhuis S, Geijtenbeek T, et al. MAVS Genetic Variation Is Associated with Decreased HIV-1 Replication In Vitro and Reduced CD4+ T Cell Infection in HIV-1-Infected Individuals. Viruses. 2020;12: pubmed publisher
Arbore G, West E, Rahman J, Le Friec G, Niyonzima N, Pirooznia M, et al. Complement receptor CD46 co-stimulates optimal human CD8+ T cell effector function via fatty acid metabolism. Nat Commun. 2018;9:4186 pubmed publisher
Sponaas A, Yang R, Rustad E, Standal T, Thoresen A, Dao Vo C, et al. PD1 is expressed on exhausted T cells as well as virus specific memory CD8+ T cells in the bone marrow of myeloma patients. Oncotarget. 2018;9:32024-32035 pubmed publisher
Li Y, Hermanson D, Moriarity B, Kaufman D. Human iPSC-Derived Natural Killer Cells Engineered with Chimeric Antigen Receptors Enhance Anti-tumor Activity. Cell Stem Cell. 2018;23:181-192.e5 pubmed publisher
Kratchmarov R, Magun A, Reiner S. TCF1 expression marks self-renewing human CD8+ T cells. Blood Adv. 2018;2:1685-1690 pubmed publisher
Vargas Hernandez A, Mace E, Zimmerman O, Zerbe C, Freeman A, Rosenzweig S, et al. Ruxolitinib partially reverses functional natural killer cell deficiency in patients with signal transducer and activator of transcription 1 (STAT1) gain-of-function mutations. J Allergy Clin Immunol. 2018;141:2142-2155.e5 pubmed publisher
Law C, Siu C, Fan K, Lai W, Au K, Lau Y, et al. Lysosomal membrane permeabilization is involved in oxidative stress-induced apoptotic cell death in LAMP2-deficient iPSCs-derived cerebral cortical neurons. Biochem Biophys Rep. 2016;5:335-345 pubmed publisher
Zhao T, Bernstein K, Fang J, Shen X. Angiotensin-converting enzyme affects the presentation of MHC class II antigens. Lab Invest. 2017;97:764-771 pubmed publisher
Aw Yeang H, Piersma S, Lin Y, Yang L, Malkova O, Miner C, et al. Cutting Edge: Human CD49e- NK Cells Are Tissue Resident in the Liver. J Immunol. 2017;198:1417-1422 pubmed publisher
Sibilano R, Gaudenzio N, DeGorter M, Reber L, Hernandez J, Starkl P, et al. A TNFRSF14-Fc?RI-mast cell pathway contributes to development of multiple features of asthma pathology in mice. Nat Commun. 2016;7:13696 pubmed publisher
Perdu S, Castellana B, Kim Y, Chan K, DeLuca L, Beristain A. Maternal obesity drives functional alterations in uterine NK cells. JCI Insight. 2016;1:e85560 pubmed publisher
Kim S, Roy S, Chen B, Nguyen T, McMonigle R, McCracken A, et al. Targeting cancer metabolism by simultaneously disrupting parallel nutrient access pathways. J Clin Invest. 2016;126:4088-4102 pubmed publisher
Tan K, Ho M, Cho H, Yu J, Hung J, Yu A. Fucosylation of LAMP-1 and LAMP-2 by FUT1 correlates with lysosomal positioning and autophagic flux of breast cancer cells. Cell Death Dis. 2016;7:e2347 pubmed publisher
Smedley J, Macalister R, Wangari S, Gathuka M, Ahrens J, Iwayama N, et al. Laparoscopic Technique for Serial Collection of Para-Colonic, Left Colic, and Inferior Mesenteric Lymph Nodes in Macaques. PLoS ONE. 2016;11:e0157535 pubmed publisher
Singh N, Kulikovskaya I, Barrett D, Binder Scholl G, Jakobsen B, Martinez D, et al. T cells targeting NY-ESO-1 demonstrate efficacy against disseminated neuroblastoma. Oncoimmunology. 2016;5:e1040216 pubmed
Dieckmann N, Hackmann Y, Aricò M, Griffiths G. Munc18-2 is required for Syntaxin 11 Localization on the Plasma Membrane in Cytotoxic T-Lymphocytes. Traffic. 2015;16:1330-41 pubmed publisher
Amancha P, Hong J, Rogers K, Ansari A, Villinger F. In vivo blockade of the programmed cell death-1 pathway using soluble recombinant PD-1-Fc enhances CD4+ and CD8+ T cell responses but has limited clinical benefit. J Immunol. 2013;191:6060-70 pubmed publisher
Zhou Q, Gil Krzewska A, Peruzzi G, Borrego F. Matrix metalloproteinases inhibition promotes the polyfunctionality of human natural killer cells in therapeutic antibody-based anti-tumour immunotherapy. Clin Exp Immunol. 2013;173:131-9 pubmed publisher
McArthur M, Sztein M. Unexpected heterogeneity of multifunctional T cells in response to superantigen stimulation in humans. Clin Immunol. 2013;146:140-52 pubmed publisher
Phuphanich S, Wheeler C, Rudnick J, Mazer M, Wang H, Nuño M, et al. Phase I trial of a multi-epitope-pulsed dendritic cell vaccine for patients with newly diagnosed glioblastoma. Cancer Immunol Immunother. 2013;62:125-35 pubmed publisher
Vargas Inchaustegui D, Xiao P, Tuero I, Patterson L, Robert Guroff M. NK and CD4+ T cell cooperative immune responses correlate with control of disease in a macaque simian immunodeficiency virus infection model. J Immunol. 2012;189:1878-85 pubmed publisher
Hazeldine J, Hampson P, Lord J. Reduced release and binding of perforin at the immunological synapse underlies the age-related decline in natural killer cell cytotoxicity. Aging Cell. 2012;11:751-9 pubmed publisher
Roat E, De Biasi S, Bertoncelli L, Rompianesi G, Nasi M, Gibellini L, et al. Immunological advantages of everolimus versus cyclosporin A in liver-transplanted recipients, as revealed by polychromatic flow cytometry. Cytometry A. 2012;81:303-11 pubmed publisher
Mattila J, Diedrich C, Lin P, Phuah J, Flynn J. Simian immunodeficiency virus-induced changes in T cell cytokine responses in cynomolgus macaques with latent Mycobacterium tuberculosis infection are associated with timing of reactivation. J Immunol. 2011;186:3527-37 pubmed publisher
Barlan A, Danthi P, Wiethoff C. Lysosomal localization and mechanism of membrane penetration influence nonenveloped virus activation of the NLRP3 inflammasome. Virology. 2011;412:306-14 pubmed publisher
Kim E, Kim T, Kim N, Kim S, Kumar V, Lee K. Homotypic cell to cell cross-talk among human natural killer cells reveals differential and overlapping roles of 2B4 and CD2. J Biol Chem. 2010;285:41755-64 pubmed publisher
Kim T, Kim N, Kang H, Kim E, Kim S, Ahn H, et al. FK506 causes cellular and functional defects in human natural killer cells. J Leukoc Biol. 2010;88:1089-97 pubmed publisher
Salerno Goncalves R, Wahid R, Sztein M. Ex Vivo kinetics of early and long-term multifunctional human leukocyte antigen E-specific CD8+ cells in volunteers immunized with the Ty21a typhoid vaccine. Clin Vaccine Immunol. 2010;17:1305-14 pubmed publisher
Peralta E, Martin B, Edinger A. Differential effects of TBC1D15 and mammalian Vps39 on Rab7 activation state, lysosomal morphology, and growth factor dependence. J Biol Chem. 2010;285:16814-21 pubmed publisher
Huynh C, Andrews N. The small chemical vacuolin-1 alters the morphology of lysosomes without inhibiting Ca2+-regulated exocytosis. EMBO Rep. 2005;6:843-7 pubmed
Betts M, Koup R. Detection of T-cell degranulation: CD107a and b. Methods Cell Biol. 2004;75:497-512 pubmed
Grützkau A, Smorodchenko A, Lippert U, Kirchhof L, Artuc M, Henz B. LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells. Cytometry A. 2004;61:62-8 pubmed
Sarafian V, Jadot M, Foidart J, Letesson J, van den Brule F, Castronovo V, et al. Expression of Lamp-1 and Lamp-2 and their interactions with galectin-3 in human tumor cells. Int J Cancer. 1998;75:105-11 pubmed
Carlsson S, Roth J, Piller F, Fukuda M. Isolation and characterization of human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Major sialoglycoproteins carrying polylactosaminoglycan. J Biol Chem. 1988;263:18911-9 pubmed
product information
Product Type :
Antibody
Product Name :
CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), eBioscience
Catalog # :
14-1079-80
Quantity :
25 µg
Price :
US 76.50
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Human
Applications :
Flow Cytometry: 0.5 µg/test, Immunocytochemistry: 5 µg/mL, Immunohistochemistry (Paraffin): 5 µg/mL, Western Blot: 0.5 µg/test
Species :
Human
Clone :
eBioH4A3
Isotype :
IgG1, kappa
Storage :
4° C
Description :
LAMP1 (CD107a, lysosome-associated membrane protein-1) together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. LAMP1 is a heavily glycosylated membrane protein which contains a putative signal peptide, 18 sites for N-linked glycosylation, a single membrane-spanning segment and a short (11 amino acid) cytosolic tail. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. LAMP1 is a type 1 integral membrane protein that is transported from trans-Golgi network to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboyxl-terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1, and this mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 (and LAMP2) have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore, they are possibly involved in the adhesion of PBMC to the site of inflammation. Increased LAMP1 immunoreactivity is observed in neurons and glial cells surrounding senile plaques in Alzheimer’s Disease (AD) cases, and is localized in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. LAMP1 is a good marker of mast cell activation.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.5 µg/test, Immunocytochemistry: 5 µg/mL, Immunohistochemistry (Paraffin): 5 µg/mL, Western Blot: 0.5 µg/test
Aliases :
120 kDa lysosomal membrane glycoprotein; AI196048; CD107 antigen-like family member A; CD107a; I79_011073; Lamp I; LAMP1; LAMP-1; LAMPA; LGP120; LGP-120; LGPA; LGP-A; lysosomal associated membrane protein 1; Lysosomal associated membrane protein 1 (120 kDa); lysosomal membrane glycoprotein 1; lysosomal membrane glycoprotein A; lysosomal-associated membrane protein 1; lysosome-associated membrane glycoprotein 1; LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEIN 1 PRECURSOR (LAMP-1) (LGP-A) (LGP-120) (CD107A) (P2B); lysosome-associated membrane protein 1; P2B
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA