CD107b (LAMP-2) Monoclonal Antibody (eBioH4B4 (H4B4)), eBioscience | Invitrogen 14-1078-80 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD107b (LAMP-2) Monoclonal Antibody (eBioH4B4 (H4B4)), eBioscience

catalog :

14-1078-80

quantity :

25 µg

price :

US 75.75

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

eBioH4B4 (H4B4)

reactivity :

human

application :

western blot, immunocytochemistry, flow cytometry

more info or order :

Invitrogen product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 4a	Dimitrova M, Zenarruzabeitia O, Borrego F, Simhadri V. CD300c is uniquely expressed on CD56 bright Natural Killer Cells and differs from CD300a upon ligand recognition. Sci Rep. 2016;6:23942 pubmed publisher
	Bojko A, Staniak K, Czarnecka Herok J, Sunderland P, Dudkowska M, Sliwińska M, et al. Improved Autophagic Flux in Escapers from Doxorubicin-Induced Senescence/Polyploidy of Breast Cancer Cells. Int J Mol Sci. 2020;21: pubmed publisher
	Law C, Siu C, Fan K, Lai W, Au K, Lau Y, et al. Lysosomal membrane permeabilization is involved in oxidative stress-induced apoptotic cell death in LAMP2-deficient iPSCs-derived cerebral cortical neurons. Biochem Biophys Rep. 2016;5:335-345 pubmed publisher
	Fonteneau J, Brilot F, MÃ¼nz C, GannagÃ© M. The Tumor Antigen NY-ESO-1 Mediates Direct Recognition of Melanoma Cells by CD4+ T Cells after Intercellular Antigen Transfer. J Immunol. 2016;196:64-71 pubmed publisher
	McArthur M, Sztein M. Unexpected heterogeneity of multifunctional T cells in response to superantigen stimulation in humans. Clin Immunol. 2013;146:140-52 pubmed publisher
	Gannage M, da Silva R, MUNZ C. Antigen processing for MHC presentation via macroautophagy. Methods Mol Biol. 2013;960:473-488 pubmed publisher
	Holmes T, El Sherbiny Y, Davison A, Clough S, Blair G, Cook G. A human NK cell activation/inhibition threshold allows small changes in the target cell surface phenotype to dramatically alter susceptibility to NK cells. J Immunol. 2011;186:1538-45 pubmed publisher
	Salerno Goncalves R, Wahid R, Sztein M. Ex Vivo kinetics of early and long-term multifunctional human leukocyte antigen E-specific CD8+ cells in volunteers immunized with the Ty21a typhoid vaccine. Clin Vaccine Immunol. 2010;17:1305-14 pubmed publisher
	Betts M, Koup R. Detection of T-cell degranulation: CD107a and b. Methods Cell Biol. 2004;75:497-512 pubmed
	Grützkau A, Smorodchenko A, Lippert U, Kirchhof L, Artuc M, Henz B. LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells. Cytometry A. 2004;61:62-8 pubmed
	Eskelinen E, Illert A, Tanaka Y, Schwarzmann G, Blanz J, von Figura K, et al. Role of LAMP-2 in lysosome biogenesis and autophagy. Mol Biol Cell. 2002;13:3355-68 pubmed
	Nishino I, Fu J, Tanji K, Yamada T, Shimojo S, Koori T, et al. Primary LAMP-2 deficiency causes X-linked vacuolar cardiomyopathy and myopathy (Danon disease). Nature. 2000;406:906-10 pubmed
	Carlsson S, Roth J, Piller F, Fukuda M. Isolation and characterization of human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Major sialoglycoproteins carrying polylactosaminoglycan. J Biol Chem. 1988;263:18911-9 pubmed

product information

Product Type :

Antibody

Product Name :

CD107b (LAMP-2) Monoclonal Antibody (eBioH4B4 (H4B4)), eBioscience

Catalog # :

14-1078-80

Quantity :

25 µg

Price :

US 75.75

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: 0.5 µg/test, Immunocytochemistry: 1:100, Western Blot: 1 µg/mL

Species :

Human

Clone :

eBioH4B4 (H4B4)

Isotype :

IgG1, kappa

Storage :

4° C

Description :

LAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b) is a member of a family of membrane glycoproteins. LAMP2 provides selectins with carbohydrate ligands, and may play a role in tumor cell metastasis, and have a function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is approximately 46 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides with a total molecular weight of about 100-130 kDa. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. LAMP2 is used to monitor a very selective form of autophagy called chaperone-mediated autophagy. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. The LAMP protein has 3 variant forms: LAMP-2A, -2B, and 2C. LAMP2, together with CD107a/LAMP-1, is a major constituent of lysosomal membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes, and LAMP2 is an important regulator in successful phagosomal maturation. LAMP2 deficiency causes an accumulation of autophagosomes in many tissues leading to cardiomyopathy and myopathy (Danon’s disease).

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.5 µg/test, Immunocytochemistry: 1:100, Western Blot: 1 µg/mL

Aliases :

CD107 antigen-like family member B; CD107b; EGK_20887; Igp96; Lamp II; LAMP2; LAMP-2; LAMP-2 Isoform 2A; LAMP-2 Variant A; Lamp-2a; Lamp-2b; Lamp-2c; LAMPB; LGP110; LGP-110; LGP-96; LGP-B; lysosomal associated membrane protein 2; lysosomal membrane glycoprotein 2; lysosomal membrane glycoprotein type B; lysosomal-associated membrane protein 2; lysosome-associated membrane glycoprotein 2; lysosome-associated membrane protein 2; Mac3; RP23-193O17.2

more info or order :

Invitrogen product webpage