CD95 (APO-1/Fas) Monoclonal Antibody (15A7), eBioscience | Invitrogen 14-0951-85 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD95 (APO-1/Fas) Monoclonal Antibody (15A7), eBioscience

catalog :

14-0951-85

quantity :

500 µg

price :

US 303.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

15A7

reactivity :

mouse

application :

flow cytometry

more info or order :

Invitrogen product webpage

citations: 52

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; 1:100; loading ...; fig 2h	Aslam M, Alemdehy M, Kwesi Maliepaard E, Muhaimin F, Caganova M, Pardieck I, et al. Histone methyltransferase DOT1L controls state-specific identity during B cell differentiation. EMBO Rep. 2021;22:e51184 pubmed publisher
flow cytometry; mouse; loading ...; fig 3e	Zhang Q, Xiang L, Zaman M, Dong W, He G, Deng G. Predominant Role of Immunoglobulin G in the Pathogenesis of Splenomegaly in Murine Lupus. Front Immunol. 2019;10:3020 pubmed publisher
flow cytometry; mouse; loading ...; fig 6a	Zhao X, Xie H, Zhao M, Ahsan A, Li X, Wang F, et al. Fc receptor-like 1 intrinsically recruits c-Abl to enhance B cell activation and function. Sci Adv. 2019;5:eaaw0315 pubmed publisher
flow cytometry; mouse; loading ...; fig 1e	Li F, Zeng Z, Xing S, Gullicksrud J, Shan Q, Choi J, et al. Ezh2 programs TFH differentiation by integrating phosphorylation-dependent activation of Bcl6 and polycomb-dependent repression of p19Arf. Nat Commun. 2018;9:5452 pubmed publisher
flow cytometry; mouse; loading ...; fig 7c	Georgiev H, Ravens I, Papadogianni G, Halle S, Malissen B, Loots G, et al. Shared and Unique Features Distinguishing Follicular T Helper and Regulatory Cells of Peripheral Lymph Node and Peyer's Patches. Front Immunol. 2018;9:714 pubmed publisher
flow cytometry; mouse; loading ...; fig 4e	Minguet S, Kläsener K, Schaffer A, Fiala G, Osteso Ibanez T, Raute K, et al. Caveolin-1-dependent nanoscale organization of the BCR regulates B cell tolerance. Nat Immunol. 2017;18:1150-1159 pubmed publisher
flow cytometry; mouse; loading ...; fig 4a	Rubtsova K, Rubtsov A, Thurman J, Mennona J, Kappler J, Marrack P. B cells expressing the transcription factor T-bet drive lupus-like autoimmunity. J Clin Invest. 2017;127:1392-1404 pubmed publisher
flow cytometry; mouse; loading ...; fig s3c	Chu V, Graf R, Wirtz T, Weber T, Favret J, Li X, et al. Efficient CRISPR-mediated mutagenesis in primary immune cells using CrispRGold and a C57BL/6 Cas9 transgenic mouse line. Proc Natl Acad Sci U S A. 2016;113:12514-12519 pubmed
flow cytometry; mouse	Choi Y, Gullicksrud J, Xing S, Zeng Z, Shan Q, Li F, et al. LEF-1 and TCF-1 orchestrate T(FH) differentiation by regulating differentiation circuits upstream of the transcriptional repressor Bcl6. Nat Immunol. 2015;16:980-90 pubmed publisher
flow cytometry; mouse	Dahlgren M, Gustafsson Hedberg T, Livingston M, Cucak H, AlsÃ©n S, Yrlid U, et al. T follicular helper, but not Th1, cell differentiation in the absence of conventional dendritic cells. J Immunol. 2015;194:5187-99 pubmed publisher
flow cytometry; mouse	Kim Y, Lim H, Jung H, Wetsel R, Chung Y. Regulation of autoimmune germinal center reactions in lupus-prone BXD2 mice by follicular helper T cells. PLoS ONE. 2015;10:e0120294 pubmed publisher
flow cytometry; mouse; fig 3	Shrestha S, Yang K, Guy C, Vogel P, Neale G, Chi H. Treg cells require the phosphatase PTEN to restrain TH1 and TFH cell responses. Nat Immunol. 2015;16:178-87 pubmed publisher
	Shen S, Li Y, Jin M, Fan D, Pan R, Lin A, et al. CD4+ CTLs Act as a Key Effector Population for Allograft Rejection of MSCs in a Donor MHC-II Dependent Manner in Injured Liver. Aging Dis. 2022;13:1919-1938 pubmed publisher
	Hendin H, Lavoie P, Gravett J, Pillet S, Saxena P, Landry N, et al. Elimination of receptor binding by influenza hemagglutinin improves vaccine-induced immunity. NPJ Vaccines. 2022;7:42 pubmed publisher
	Kim H, Kim Y, Park W. Berberine modulates hyper-inflammation in mouse macrophages stimulated with polyinosinic-polycytidylic acid via calcium-CHOP/STAT pathway. Sci Rep. 2021;11:11298 pubmed publisher
	Huang X, Hao S, Liu J, Huang Y, Liu M, Xiao C, et al. The ubiquitin ligase Peli1 inhibits ICOS and thereby Tfh-mediated immunity. Cell Mol Immunol. 2021;18:969-978 pubmed publisher
	Xu W, Snell L, Guo M, Boukhaled G, Macleod B, Li M, et al. Early innate and adaptive immune perturbations determine long-term severity of chronic virus and Mycobacterium tuberculosis coinfection. Immunity. 2021;54:526-541.e7 pubmed publisher
	Melo Gonzalez F, Kammoun H, Evren E, Dutton E, Papadopoulou M, Bradford B, et al. Antigen-presenting ILC3 regulate T cell-dependent IgA responses to colonic mucosal bacteria. J Exp Med. 2019;216:728-742 pubmed publisher
	Liu J, Huang X, Hao S, Wang Y, Liu M, Xu J, et al. Peli1 negatively regulates noncanonical NF-κB signaling to restrain systemic lupus erythematosus. Nat Commun. 2018;9:1136 pubmed publisher
	Gaya M, Barral P, Burbage M, Aggarwal S, Montaner B, Warren Navia A, et al. Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells. Cell. 2018;172:517-533.e20 pubmed publisher
	YEKU O, Purdon T, Koneru M, Spriggs D, Brentjens R. Armored CAR T cells enhance antitumor efficacy and overcome the tumor microenvironment. Sci Rep. 2017;7:10541 pubmed publisher
	Miletić A, Lenartic M, Popovic B, Brizic I, Trsan T, Miklic K, et al. NCR1-deficiency diminishes the generation of protective murine cytomegalovirus antibodies by limiting follicular helper T-cell maturation. Eur J Immunol. 2017;47:1443-1456 pubmed publisher
	Kleiman E, Jia H, Loguercio S, Su A, Feeney A. YY1 plays an essential role at all stages of B-cell differentiation. Proc Natl Acad Sci U S A. 2016;113:E3911-20 pubmed publisher
	Huang B, Gomez Rodriguez J, Preite S, Garrett L, Harper U, Schwartzberg P. CRISPR-Mediated Triple Knockout of SLAMF1, SLAMF5 and SLAMF6 Supports Positive Signaling Roles in NKT Cell Development. PLoS ONE. 2016;11:e0156072 pubmed publisher
	Xu X, Jin R, Li M, Wang K, Zhang S, Hao J, et al. Liver sinusoidal endothelial cells induce tolerance of autoreactive CD4+ recent thymic emigrants. Sci Rep. 2016;6:19861 pubmed publisher
	Matino D, Gargaro M, Santagostino E, Di Minno M, Castaman G, Morfini M, et al. IDO1 suppresses inhibitor development in hemophilia A treated with factor VIII. J Clin Invest. 2015;125:3766-81 pubmed publisher
	Ortega Molina A, Boss I, Canela A, Pan H, Jiang Y, Zhao C, et al. The histone lysine methyltransferase KMT2D sustains a gene expression program that represses B cell lymphoma development. Nat Med. 2015;21:1199-208 pubmed publisher
	Desvignes L, Weidinger C, Shaw P, Vaeth M, Ribierre T, Liu M, et al. STIM1 controls T cell-mediated immune regulation and inflammation in chronic infection. J Clin Invest. 2015;125:2347-62 pubmed publisher
	Zhao H, Bauzon F, Bi E, Yu J, Fu H, Lu Z, et al. Substituting threonine 187 with alanine in p27Kip1 prevents pituitary tumorigenesis by two-hit loss of Rb1 and enhances humoral immunity in old age. J Biol Chem. 2015;290:5797-809 pubmed publisher
	Ding Y, Li J, Wu Q, Yang P, Luo B, Xie S, et al. IL-17RA is essential for optimal localization of follicular Th cells in the germinal center light zone to promote autoantibody-producing B cells. J Immunol. 2013;191:1614-24 pubmed publisher
	Chu Y, Soberon V, Glockner L, Beyaert R, Massoumi R, Van Loo G, et al. A20 and CYLD do not share significant overlapping functions during B cell development and activation. J Immunol. 2012;189:4437-43 pubmed publisher
	Nakagawa Y, Shimizu M, Norose Y, Takahashi M, Takahashi H. Induction of rapid apoptosis for class I MHC molecule-restricted CD8(+) HIV-1 gp160-specific murine activated CTLs by free antigenic peptide in vivo. Int Immunol. 2013;25:11-24 pubmed publisher
	Garchow B, Bartulos Encinas O, Leung Y, Tsao P, Eisenberg R, Caricchio R, et al. Silencing of microRNA-21 in vivo ameliorates autoimmune splenomegaly in lupus mice. EMBO Mol Med. 2011;3:605-15 pubmed publisher
	Green B, Belcheva A, Nepal R, Boulianne B, Martin A. The mismatch repair pathway functions normally at a non-AID target in germinal center B cells. Blood. 2011;118:3013-8 pubmed publisher
	Tabbekh M, Franciszkiewicz K, Haouas H, Lecluse Y, Benihoud K, Raman C, et al. Rescue of tumor-infiltrating lymphocytes from activation-induced cell death enhances the antitumor CTL response in CD5-deficient mice. J Immunol. 2011;187:102-9 pubmed publisher
	Huang F, Barnes P, Feng Y, Donis R, Chroneos Z, Idell S, et al. GM-CSF in the lung protects against lethal influenza infection. Am J Respir Crit Care Med. 2011;184:259-68 pubmed publisher
	Chu Y, Vahl J, Kumar D, Heger K, Bertossi A, Wójtowicz E, et al. B cells lacking the tumor suppressor TNFAIP3/A20 display impaired differentiation and hyperactivation and cause inflammation and autoimmunity in aged mice. Blood. 2011;117:2227-36 pubmed publisher
	Pearl Yafe M, Mizrahi K, Stein J, Yolcu E, Kaplan O, Shirwan H, et al. Tumor necrosis factor receptors support murine hematopoietic progenitor function in the early stages of engraftment. Stem Cells. 2010;28:1270-80 pubmed publisher
	Bolduc A, Long E, Stapler D, Cascalho M, Tsubata T, Koni P, et al. Constitutive CD40L expression on B cells prematurely terminates germinal center response and leads to augmented plasma cell production in T cell areas. J Immunol. 2010;185:220-30 pubmed publisher
	Zaheen A, Boulianne B, Parsa J, Ramachandran S, Gommerman J, Martin A. AID constrains germinal center size by rendering B cells susceptible to apoptosis. Blood. 2009;114:547-54 pubmed publisher
	Lee S, Salek Ardakani S, Mittler R, Croft M. Hypercostimulation through 4-1BB distorts homeostasis of immune cells. J Immunol. 2009;182:6753-62 pubmed publisher
	Mise Omata S, Kuroda E, Sugiura T, Yamashita U, Obata Y, Doi T. The NF-kappaB RelA subunit confers resistance to Leishmania major by inducing nitric oxide synthase 2 and Fas expression but not Th1 differentiation. J Immunol. 2009;182:4910-6 pubmed publisher
	Nepal R, Zaheen A, Basit W, Li L, Berger S, Martin A. AID and RAG1 do not contribute to lymphomagenesis in Emu c-myc transgenic mice. Oncogene. 2008;27:4752-6 pubmed publisher
	Treese C, Mittag A, Lange F, Tarnok A, Loesche A, Emmrich F, et al. Characterization of fibroblasts responsible for cartilage destruction in arthritis. Cytometry A. 2008;73:351-60 pubmed publisher
	Pearl Yafe M, Yolcu E, Stein J, Kaplan O, Shirwan H, Yaniv I, et al. Expression of Fas and Fas-ligand in donor hematopoietic stem and progenitor cells is dissociated from the sensitivity to apoptosis. Exp Hematol. 2007;35:1601-12 pubmed
	Pearl Yafe M, Stein J, Yolcu E, Farkas D, Shirwan H, Yaniv I, et al. Fas transduces dual apoptotic and trophic signals in hematopoietic progenitors. Stem Cells. 2007;25:3194-203 pubmed
	Li M, Wang Y, Guo R, Bai Y, Yu Z. Glucocorticoids impair microglia ability to induce T cell proliferation and Th1 polarization. Immunol Lett. 2007;109:129-37 pubmed
	Peshes Yaloz N, Rosen D, Sondel P, Krammer P, Berke G. Up-regulation of Fas (CD95) expression in tumour cells in vivo. Immunology. 2007;120:502-11 pubmed
	Aloman C, Gehring S, Wintermeyer P, Kuzushita N, Wands J. Chronic ethanol consumption impairs cellular immune responses against HCV NS5 protein due to dendritic cell dysfunction. Gastroenterology. 2007;132:698-708 pubmed
	Podojil J, Kohm A, Miller S. CD4+ T cell expressed CD80 regulates central nervous system effector function and survival during experimental autoimmune encephalomyelitis. J Immunol. 2006;177:2948-58 pubmed
	Yonehara S. Effects of anti-Fas antibodies on lymphocytes and other organs: preparation of original and new monoclonal antibodies and amelioration of systemic autoimmune disease. Int Rev Immunol. 1999;18:329-45 pubmed
	Nishimura Y, Ishii A, Kobayashi Y, Yamasaki Y, Yonehara S. Expression and function of mouse Fas antigen on immature and mature T cells. J Immunol. 1995;154:4395-403 pubmed

product information

Product Type :

Antibody

Product Name :

CD95 (APO-1/Fas) Monoclonal Antibody (15A7), eBioscience

Catalog # :

14-0951-85

Quantity :

500 µg

Price :

US 303.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Mouse

Applications :

Flow Cytometry: 1 µg/test

Species :

Mouse

Clone :

15A7

Isotype :

IgG1, kappa

Storage :

4° C

Description :

FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. FAS also exists in a soluble form that weight approximately 26kDa. Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases. The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis. FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform. The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer. It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1 µg/test

Aliases :

AI196731; ALPS1A; APO1; APO-1; Apo1 antigen; Apo-1 antigen; APO-1 cell surface antigen; Apo-1 Fas; apoptosis antigen 1; apoptosis signaling receptor FAS; apoptosis-mediating surface antigen FAS; APT1; CD95; CD95 antigen; FAS; Fas (TNF receptor superfamily member 6); Fas (TNF receptor superfamily member); Fas (TNF receptor superfamily, member 6); Fas AMA; Fas antigen; Fas antigen (ATP1); Fas cell surface death receptor; Fas receptor; FAS1; FASLG receptor; FASTM; lpr; lymphoproliferation; OTTHUMP00000020045; OTTHUMP00000020046; OTTHUMP00000020051; OTTHUMP00000059646; TNF receptor superfamily member 6; TNFR6; Tnfrsf6; tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily member 26; Tumor necrosis factor receptor superfamily, member 6

more info or order :

Invitrogen product webpage