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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD69 Monoclonal Antibody (FN50), eBioscience™
catalog :
14-0699-82
quantity :
100µg
price :
US 101
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
FN50
reactivity :
African green monkey, human
application :
immunohistochemistry, flow cytometry
more info or order :
citations: 63
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 1a
Terahara K, Iwabuchi R, Hosokawa M, Nishikawa Y, Takeyama H, Takahashi Y, et al. A CCR5+ memory subset within HIV-1-infected primary resting CD4+ T cells is permissive for replication-competent, latently infected viruses in vitro. BMC Res Notes. 2019;12:242 pubmed publisher
  • flow cytometry; human; loading ...; fig 5a
Lim S, Kim J, Jeon S, Shin M, Kwon J, Kim T, et al. Defective Localization With Impaired Tumor Cytotoxicity Contributes to the Immune Escape of NK Cells in Pancreatic Cancer Patients. Front Immunol. 2019;10:496 pubmed publisher
  • flow cytometry; human; fig 1
Yin W, Tong S, Zhang Q, Shao J, Liu Q, Peng H, et al. Functional dichotomy of Vδ2 γδ T cells in chronic hepatitis C virus infections: role in cytotoxicity but not for IFN-γ production. Sci Rep. 2016;6:26296 pubmed publisher
  • flow cytometry; human; fig s2
Woo J, Srikanth S, Nishi M, Ping P, Takeshima H, Gwack Y. Junctophilin-4, a component of the endoplasmic reticulum-plasma membrane junctions, regulates Ca2+ dynamics in T cells. Proc Natl Acad Sci U S A. 2016;113:2762-7 pubmed publisher
  • flow cytometry; human; fig 1
Owens G, Erickson K, Malone C, Pan C, Huynh M, Chang J, et al. Evidence for the involvement of gamma delta T cells in the immune response in Rasmussen encephalitis. J Neuroinflammation. 2015;12:134 pubmed publisher
  • flow cytometry; human; fig 1
Severson J, Serracino H, Mateescu V, Raeburn C, McIntyre R, Sams S, et al. PD-1+Tim-3+ CD8+ T Lymphocytes Display Varied Degrees of Functional Exhaustion in Patients with Regionally Metastatic Differentiated Thyroid Cancer. Cancer Immunol Res. 2015;3:620-30 pubmed publisher
  • flow cytometry; human
Willmann K, Klaver S, DoÄŸu F, Santos Valente E, Garncarz W, Bilic I, et al. Biallelic loss-of-function mutation in NIK causes a primary immunodeficiency with multifaceted aberrant lymphoid immunity. Nat Commun. 2014;5:5360 pubmed publisher
  • flow cytometry; African green monkey; fig 4
Jacquelin B, Petitjean G, Kunkel D, Liovat A, Jochems S, Rogers K, et al. Innate immune responses and rapid control of inflammation in African green monkeys treated or not with interferon-alpha during primary SIVagm infection. PLoS Pathog. 2014;10:e1004241 pubmed publisher
  • flow cytometry; human
Zouk H, d Hennezel E, Du X, Ounissi Benkalha H, Piccirillo C, Polychronakos C. Functional evaluation of the role of C-type lectin domain family 16A at the chromosome 16p13 locus. Clin Exp Immunol. 2014;175:485-97 pubmed publisher
  • flow cytometry; human; fig 5
Daigneault M, de Silva T, Bewley M, Preston J, Marriott H, Mitchell A, et al. Monocytes regulate the mechanism of T-cell death by inducing Fas-mediated apoptosis during bacterial infection. PLoS Pathog. 2012;8:e1002814 pubmed publisher
  • flow cytometry; human
Sivasankar B, Longhi M, Gallagher K, Betts G, Morgan B, Godkin A, et al. CD59 blockade enhances antigen-specific CD4+ T cell responses in humans: a new target for cancer immunotherapy?. J Immunol. 2009;182:5203-7 pubmed publisher
Albert B, Niu A, Ramani R, Marshall G, Wender P, Williams R, et al. Combinations of isoform-targeted histone deacetylase inhibitors and bryostatin analogues display remarkable potency to activate latent HIV without global T-cell activation. Sci Rep. 2017;7:7456 pubmed publisher
Houng J, Tai T, Hsu S, Hsu H, Hwang T, Lin C, et al. Glossogyne tenuifolia (Hsiang-ju) extract suppresses T cell activation by inhibiting activation of c-Jun N-terminal kinase. Chin Med. 2017;12:9 pubmed publisher
Oberg H, Grage Griebenow E, Adam Klages S, Jerg E, Peipp M, Kellner C, et al. Monitoring and functional characterization of the lymphocytic compartment in pancreatic ductal adenocarcinoma patients. Pancreatology. 2016;16:1069-1079 pubmed publisher
van Wilgenburg B, Scherwitzl I, Hutchinson E, Leng T, Kurioka A, Kulicke C, et al. MAIT cells are activated during human viral infections. Nat Commun. 2016;7:11653 pubmed publisher
Jozwik A, Habibi M, Paras A, Zhu J, Guvenel A, Dhariwal J, et al. RSV-specific airway resident memory CD8+ T cells and differential disease severity after experimental human infection. Nat Commun. 2015;6:10224 pubmed publisher
Reeder J, Kwak Y, McNamara R, Forst C, D Orso I. HIV Tat controls RNA Polymerase II and the epigenetic landscape to transcriptionally reprogram target immune cells. elife. 2015;4: pubmed publisher
Taborda N, González S, Alvarez C, Correa L, Montoya C, Rugeles M. Higher Frequency of NK and CD4+ T-Cells in Mucosa and Potent Cytotoxic Response in HIV Controllers. PLoS ONE. 2015;10:e0136292 pubmed publisher
Magalhaes I, Pingris K, Poitou C, Bessoles S, Venteclef N, Kiaf B, et al. Mucosal-associated invariant T cell alterations in obese and type 2 diabetic patients. J Clin Invest. 2015;125:1752-62 pubmed publisher
Guo Y, Riley K, Iwasaki A, Steitz J. Alternative capture of noncoding RNAs or protein-coding genes by herpesviruses to alter host T cell function. Mol Cell. 2014;54:67-79 pubmed publisher
Legat A, Speiser D, Pircher H, Zehn D, Fuertes Marraco S. Inhibitory Receptor Expression Depends More Dominantly on Differentiation and Activation than "Exhaustion" of Human CD8?T Cells. Front Immunol. 2013;4:455 pubmed publisher
Tittarelli A, Mendoza Naranjo A, Farías M, Guerrero I, Ihara F, Wennerberg E, et al. Gap junction intercellular communications regulate NK cell activation and modulate NK cytotoxic capacity. J Immunol. 2014;192:1313-9 pubmed publisher
Sutavani R, Bradley R, Ramage J, Jackson A, Durrant L, Spendlove I. CD55 costimulation induces differentiation of a discrete T regulatory type 1 cell population with a stable phenotype. J Immunol. 2013;191:5895-903 pubmed publisher
Lin G, Field J, Yu J, Ken R, Neuberg D, Nathan D, et al. NF-?B is activated in CD4+ iNKT cells by sickle cell disease and mediates rapid induction of adenosine A2A receptors. PLoS ONE. 2013;8:e74664 pubmed publisher
Ji N, Somanaboeina A, Dixit A, Kawamura K, Hayward N, Self C, et al. Small molecule inhibitor of antigen binding and presentation by HLA-DR2b as a therapeutic strategy for the treatment of multiple sclerosis. J Immunol. 2013;191:5074-84 pubmed publisher
Yanagisawa K, Yue S, van der Vliet H, Wang R, Alatrakchi N, Golden Mason L, et al. Ex vivo analysis of resident hepatic pro-inflammatory CD1d-reactive T cells and hepatocyte surface CD1d expression in hepatitis C. J Viral Hepat. 2013;20:556-65 pubmed publisher
Liu X, Gao N, Li M, Xu D, Hou Y, Wang Q, et al. Elevated levels of CD4(+)CD25(+)FoxP3(+) T cells in systemic sclerosis patients contribute to the secretion of IL-17 and immunosuppression dysfunction. PLoS ONE. 2013;8:e64531 pubmed publisher
Ohira M, Nishida S, Matsuura T, Muraoka I, Tryphonopoulos P, Fan J, et al. Comparative analysis of T-cell depletion method for clinical immunotherapy-anti-hepatitis c effects of natural killer cells via interferon-? production. Transplant Proc. 2013;45:2045-50 pubmed publisher
Lim S, Kim T, Lee J, Sonn C, Kim K, Kim J, et al. Ex vivo expansion of highly cytotoxic human NK cells by cocultivation with irradiated tumor cells for adoptive immunotherapy. Cancer Res. 2013;73:2598-607 pubmed publisher
Barnig C, Cernadas M, Dutile S, Liu X, Perrella M, Kazani S, et al. Lipoxin A4 regulates natural killer cell and type 2 innate lymphoid cell activation in asthma. Sci Transl Med. 2013;5:174ra26 pubmed publisher
Goudy K, Aydin D, Barzaghi F, Gambineri E, Vignoli M, Ciullini Mannurita S, et al. Human IL2RA null mutation mediates immunodeficiency with lymphoproliferation and autoimmunity. Clin Immunol. 2013;146:248-61 pubmed publisher
Koristka S, Cartellieri M, Arndt C, Bippes C, Feldmann A, Michalk I, et al. Retargeting of regulatory T cells to surface-inducible autoantigen La/SS-B. J Autoimmun. 2013;42:105-16 pubmed publisher
McArthur M, Sztein M. Unexpected heterogeneity of multifunctional T cells in response to superantigen stimulation in humans. Clin Immunol. 2013;146:140-52 pubmed publisher
Cosgrove C, Ussher J, Rauch A, Gärtner K, Kurioka A, Hühn M, et al. Early and nonreversible decrease of CD161++ /MAIT cells in HIV infection. Blood. 2013;121:951-61 pubmed publisher
Ramstead A, Schepetkin I, Quinn M, Jutila M. Oenothein B, a cyclic dimeric ellagitannin isolated from Epilobium angustifolium, enhances IFN? production by lymphocytes. PLoS ONE. 2012;7:e50546 pubmed publisher
Ribot J, deBarros A, Mancio Silva L, Pamplona A, Silva Santos B. B7-CD28 costimulatory signals control the survival and proliferation of murine and human ?? T cells via IL-2 production. J Immunol. 2012;189:1202-8 pubmed publisher
Hamilton E, Pearce L, Morgan L, Robinson S, Ware V, Brennan P, et al. Mimicking the tumour microenvironment: three different co-culture systems induce a similar phenotype but distinct proliferative signals in primary chronic lymphocytic leukaemia cells. Br J Haematol. 2012;158:589-99 pubmed publisher
Qin Y, Li Y, Jiang A, Jiang J, Wang J. Stimulation of Cryptococcus neoformans isolated from skin lesion of AIDS patient matures dendritic cells and promotes HIV-1 trans-infection. Biochem Biophys Res Commun. 2012;423:709-14 pubmed publisher
McArthur M, Sztein M. Heterogeneity of multifunctional IL-17A producing S. Typhi-specific CD8+ T cells in volunteers following Ty21a typhoid immunization. PLoS ONE. 2012;7:e38408 pubmed publisher
de Kivit S, Saeland E, Kraneveld A, van de Kant H, Schouten B, van Esch B, et al. Galectin-9 induced by dietary synbiotics is involved in suppression of allergic symptoms in mice and humans. Allergy. 2012;67:343-52 pubmed publisher
Rossi L, Avila D, Spallanzani R, Ziblat A, Fuertes M, Lapyckyj L, et al. Histone deacetylase inhibitors impair NK cell viability and effector functions through inhibition of activation and receptor expression. J Leukoc Biol. 2012;91:321-31 pubmed publisher
Qin Y, Li Y, Liu W, Tian R, Guo Q, Li S, et al. Penicillium marneffei-stimulated dendritic cells enhance HIV-1 trans-infection and promote viral infection by activating primary CD4+ T cells. PLoS ONE. 2011;6:e27609 pubmed publisher
Hayworth J, Mazzuca D, Maleki Vareki S, Welch I, McCormick J, Haeryfar S. CD1d-independent activation of mouse and human iNKT cells by bacterial superantigens. Immunol Cell Biol. 2012;90:699-709 pubmed publisher
Tjin E, Konijnenberg D, Krebbers G, Mallo H, Drijfhout J, Franken K, et al. T-cell immune function in tumor, skin, and peripheral blood of advanced stage melanoma patients: implications for immunotherapy. Clin Cancer Res. 2011;17:5736-47 pubmed publisher
Correia D, Fogli M, Hudspeth K, da Silva M, Mavilio D, Silva Santos B. Differentiation of human peripheral blood V?1+ T cells expressing the natural cytotoxicity receptor NKp30 for recognition of lymphoid leukemia cells. Blood. 2011;118:992-1001 pubmed publisher
Chung J, Shiue L, Duvic M, Pandya A, Cruz P, Ariizumi K. Sézary syndrome cells overexpress syndecan-4 bearing distinct heparan sulfate moieties that suppress T-cell activation by binding DC-HIL and trapping TGF-beta on the cell surface. Blood. 2011;117:3382-90 pubmed publisher
Mannik L, Chin Yee I, Sharif S, Van Kaer L, Delovitch T, Haeryfar S. Engagement of glycosylphosphatidylinositol-anchored proteins results in enhanced mouse and human invariant natural killer T cell responses. Immunology. 2011;132:361-75 pubmed publisher
Vagenas P, Aravantinou M, Williams V, Jasny E, Piatak M, Lifson J, et al. A tonsillar PolyICLC/AT-2 SIV therapeutic vaccine maintains low viremia following antiretroviral therapy cessation. PLoS ONE. 2010;5:e12891 pubmed publisher
Llanos Cuentas A, Calderon W, Cruz M, Ashman J, Alves F, Coler R, et al. A clinical trial to evaluate the safety and immunogenicity of the LEISH-F1+MPL-SE vaccine when used in combination with sodium stibogluconate for the treatment of mucosal leishmaniasis. Vaccine. 2010;28:7427-35 pubmed publisher
Kim T, Kim N, Kang H, Kim E, Kim S, Ahn H, et al. FK506 causes cellular and functional defects in human natural killer cells. J Leukoc Biol. 2010;88:1089-97 pubmed publisher
Freeman C, Han M, Martinez F, Murray S, Liu L, Chensue S, et al. Cytotoxic potential of lung CD8(+) T cells increases with chronic obstructive pulmonary disease severity and with in vitro stimulation by IL-18 or IL-15. J Immunol. 2010;184:6504-13 pubmed publisher
Diedrich C, Mattila J, Klein E, Janssen C, Phuah J, Sturgeon T, et al. Reactivation of latent tuberculosis in cynomolgus macaques infected with SIV is associated with early peripheral T cell depletion and not virus load. PLoS ONE. 2010;5:e9611 pubmed publisher
Freeman C, Martinez F, Han M, Ames T, Chensue S, Todt J, et al. Lung dendritic cell expression of maturation molecules increases with worsening chronic obstructive pulmonary disease. Am J Respir Crit Care Med. 2009;180:1179-88 pubmed publisher
Bloom D, Chang Z, Pauly K, Kwun J, Fechner J, Hayes C, et al. BAFF is increased in renal transplant patients following treatment with alemtuzumab. Am J Transplant. 2009;9:1835-45 pubmed publisher
Nagata S, Ise T, Pastan I. Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells. J Immunol. 2009;182:7518-26 pubmed publisher
Cognasse F, Hamzeh Cognasse H, Lafarge S, Acquart S, Chavarin P, Courbil R, et al. Donor platelets stored for at least 3 days can elicit activation marker expression by the recipient's blood mononuclear cells: an in vitro study. Transfusion. 2009;49:91-8 pubmed publisher
Thakral D, Dobbins J, Devine L, Kavathas P. Differential expression of the human CD8beta splice variants and regulation of the M-2 isoform by ubiquitination. J Immunol. 2008;180:7431-42 pubmed
Lim H, Lee J, Hillsamer P, Kim C. Human Th17 cells share major trafficking receptors with both polarized effector T cells and FOXP3+ regulatory T cells. J Immunol. 2008;180:122-9 pubmed
Sumpter B, Dunham R, Gordon S, Engram J, Hennessy M, Kinter A, et al. Correlates of preserved CD4(+) T cell homeostasis during natural, nonpathogenic simian immunodeficiency virus infection of sooty mangabeys: implications for AIDS pathogenesis. J Immunol. 2007;178:1680-91 pubmed
Caudy A, Reddy S, Chatila T, Atkinson J, Verbsky J. CD25 deficiency causes an immune dysregulation, polyendocrinopathy, enteropathy, X-linked-like syndrome, and defective IL-10 expression from CD4 lymphocytes. J Allergy Clin Immunol. 2007;119:482-7 pubmed
Zhao Y, Huang Z, Qi M, Lazzarini P, Mazzone T. Immune regulation of T lymphocyte by a newly characterized human umbilical cord blood stem cell. Immunol Lett. 2007;108:78-87 pubmed
Pfistershammer K, Klauser C, Pickl W, Stockl J, Leitner J, Zlabinger G, et al. No evidence for dualism in function and receptors: PD-L2/B7-DC is an inhibitory regulator of human T cell activation. Eur J Immunol. 2006;36:1104-13 pubmed
Chan S, Olson J, Utz P. Single-cell analysis of siRNA-mediated gene silencing using multiparameter flow cytometry. Cytometry A. 2006;69:59-65 pubmed
product information
Product Type :
Antibody
Product Name :
CD69 Monoclonal Antibody (FN50), eBioscience™
Catalog # :
14-0699-82
Quantity :
100µg
Price :
US 101
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Human
Applications :
Flow Cytometry: 0.5 µg/test, Immunohistochemistry: Assay-Dependent
Species :
Human
Clone :
FN50
Isotype :
IgG1, kappa
Storage :
4° C
Description :
CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.5 µg/test, Immunohistochemistry: Assay-Dependent
Aliases :
5830438K24Rik; Activation inducer molecule; activation inducer molecule (AIM/CD69); AI452015; AIM; BL-AC/P26; Cd69; CD69 antigen; CD69 antigen (p60, early T-cell activation antigen); Cd69 molecule; CLEC2C; C-type lectin domain family 2 member C; C-type lectin domain family 2, member C; EA1; Early activation antigen CD69; early lymphocyte activation antigen; Early T-cell activation antigen p60; GP32/28; Leukocyte surface antigen Leu-23; MLR-3; VEA; Very Early Activation Antigen
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA