CD62E (E-selectin) Monoclonal Antibody (P2H3), eBioscience™ | Invitrogen 14-0627-82 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD62E (E-selectin) Monoclonal Antibody (P2H3), eBioscience™

catalog :

14-0627-82

quantity :

100 µg

price :

US 309.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

P2H3

reactivity :

human, mouse, pigs

application :

western blot, immunohistochemistry, immunoprecipitation, flow cytometry, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
blocking or activating experiments; pigs	Lo C, Weil B, Palka B, Momeni A, Canty J, Neelamegham S. Cell surface glycoengineering improves selectin-mediated adhesion of mesenchymal stem cells (MSCs) and cardiosphere-derived cells (CDCs): Pilot validation in porcine ischemia-reperfusion model. Biomaterials. 2016;74:19-30 pubmed publisher
flow cytometry; human; fig 5	Denecke B, Horsch L, Radtke S, Fischer J, Horn P, Giebel B. Human endothelial colony-forming cells expanded with an improved protocol are a useful endothelial cell source for scaffold-based tissue engineering. J Tissue Eng Regen Med. 2015;9:E84-97 pubmed publisher
	Stolfa G, Mondal N, Zhu Y, Yu X, Buffone A, Neelamegham S. Using CRISPR-Cas9 to quantify the contributions of O-glycans, N-glycans and Glycosphingolipids to human leukocyte-endothelium adhesion. Sci Rep. 2016;6:30392 pubmed publisher
	An N, Andrukhov O, Tang Y, Falkensammer F, Bantleon H, Ouyang X, et al. Effect of nicotine and porphyromonas gingivalis lipopolysaccharide on endothelial cells in vitro. PLoS ONE. 2014;9:e96942 pubmed publisher
	Stoler Barak L, Barzilai S, Zauberman A, Alon R. Transendothelial migration of effector T cells across inflamed endothelial barriers does not require heparan sulfate proteoglycans. Int Immunol. 2014;26:315-24 pubmed publisher
	Bevilacqua M, Stengelin S, Gimbrone M, Seed B. Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins. Science. 1989;243:1160-5 pubmed

product information

Product Type :

Antibody

Product Name :

CD62E (E-selectin) Monoclonal Antibody (P2H3), eBioscience™

Catalog # :

14-0627-82

Quantity :

100 µg

Price :

US 309.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

Flow Cytometry: 1 µg/test, Functional assay: Assay-Dependent, Immunohistochemistry: Assay-Dependent, Immunoprecipitation: Assay-Dependent, Western Blot: 1:500

Species :

Human, Mouse

Clone :

P2H3

Isotype :

IgG1

Storage :

4° C

Description :

Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1 µg/test, Functional assay: Assay-Dependent, Immunohistochemistry: Assay-Dependent, Immunoprecipitation: Assay-Dependent, Western Blot: 1:500

Aliases :

CD62 antigen-like family member E; CD62E; e selectin; Elam; ELAM1; ELAM-1; endothelial adhesion molecule 1; endothelial leukocyte adhesion molecule; endothelial leukocyte adhesion molecule 1; endothelial leukocyte adhesion molecule I; ESEL; E-selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; leukocyte-endothelial cell adhesion molecule 2; RP1-117P20.2; SELE; selectin E; selectin E (endothelial adhesion molecule 1); selectin, endothelial cell

more info or order :

Invitrogen product webpage