CD47 Monoclonal Antibody (B6H12), eBioscience™ | Invitrogen 14-0479-80 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD47 Monoclonal Antibody (B6H12), eBioscience™

catalog :

14-0479-80

quantity :

25 µg

price :

US 309.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

B6H12

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 19

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 1d flow cytometry; mouse; loading ...; fig 1c	Myers D, Abram C, Wildes D, Belwafa A, Welsh A, Schulze C, et al. Shp1 Loss Enhances Macrophage Effector Function and Promotes Anti-Tumor Immunity. Front Immunol. 2020;11:576310 pubmed publisher
immunocytochemistry; human; 1:100; loading ...; fig 5a	Guillon J, Petit C, Moreau M, Toutain B, Henry C, Roche H, et al. Regulation of senescence escape by TSP1 and CD47 following chemotherapy treatment. Cell Death Dis. 2019;10:199 pubmed publisher
flow cytometry; mouse; loading ...; fig e2a	Chen J, Zhong M, Guo H, Davidson D, Mishel S, Lu Y, et al. SLAMF7 is critical for phagocytosis of haematopoietic tumour cells via Mac-1 integrin. Nature. 2017;544:493-497 pubmed publisher
blocking or activating experiments; mouse; 20 ug/ml	Reed Geaghan E, Savage J, Hise A, Landreth G. CD14 and toll-like receptors 2 and 4 are required for fibrillar A{beta}-stimulated microglial activation. J Neurosci. 2009;29:11982-92 pubmed publisher
	Mair B, Aldridge P, Atwal R, Philpott D, Zhang M, Masud S, et al. High-throughput genome-wide phenotypic screening via immunomagnetic cell sorting. Nat Biomed Eng. 2019;3:796-805 pubmed publisher
	Logtenberg M, Jansen J, Raaben M, Toebes M, Franke K, Brandsma A, et al. Glutaminyl cyclase is an enzymatic modifier of the CD47- SIRPα axis and a target for cancer immunotherapy. Nat Med. 2019;25:612-619 pubmed publisher
	Mendt M, Kamerkar S, Sugimoto H, McAndrews K, Wu C, Gagea M, et al. Generation and testing of clinical-grade exosomes for pancreatic cancer. JCI Insight. 2018;3: pubmed publisher
	Hain A, KrÃ¤mer M, Linka R, Nakhaei Rad S, Ahmadian M, HÃ¤ussinger D, et al. IL-2 Inducible Kinase ITK is Critical for HIV-1 Infection of Jurkat T-cells. Sci Rep. 2018;8:3217 pubmed publisher
	Kamerkar S, LeBleu V, Sugimoto H, Yang S, Ruivo C, Melo S, et al. Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer. Nature. 2017;546:498-503 pubmed publisher
	Liu R, Wei H, Gao P, Yu H, Wang K, Fu Z, et al. CD47 promotes ovarian cancer progression by inhibiting macrophage phagocytosis. Oncotarget. 2017;8:39021-39032 pubmed publisher
	Ponce L, Fenn N, Moritz N, Krupka C, Kozik J, Lauber K, et al. SIRPα-antibody fusion proteins stimulate phagocytosis and promote elimination of acute myeloid leukemia cells. Oncotarget. 2017;8:11284-11301 pubmed publisher
	Hu C, Fang R, Wang K, Luk B, Thamphiwatana S, Dehaini D, et al. Nanoparticle biointerfacing by platelet membrane cloaking. Nature. 2015;526:118-21 pubmed publisher
	Yoshida K, Tsujimoto H, Matsumura K, Kinoshita M, Takahata R, Matsumoto Y, et al. CD47 is an adverse prognostic factor and a therapeutic target in gastric cancer. Cancer Med. 2015;4:1322-33 pubmed publisher
	Fischer N, Elson G, Magistrelli G, Dheilly E, Fouque N, Laurendon A, et al. Exploiting light chains for the scalable generation and platform purification of native human bispecific IgG. Nat Commun. 2015;6:6113 pubmed publisher
	Kaur S, Chang T, Singh S, Lim L, Mannan P, Garfield S, et al. CD47 signaling regulates the immunosuppressive activity of VEGF in T cells. J Immunol. 2014;193:3914-24 pubmed publisher
	Koido S, Homma S, Okamoto M, Namiki Y, Takakura K, Takahara A, et al. Augmentation of antitumor immunity by fusions of ethanol-treated tumor cells and dendritic cells stimulated via dual TLRs through TGF-?1 blockade and IL-12p70 production. PLoS ONE. 2013;8:e63498 pubmed publisher
	Bandyopadhyay G, Bankey P, Miller Graziano C. Trauma patients' elevated tumor necrosis related apoptosis inducing ligand (TRAIL) contributes to increased T cell apoptosis. Clin Immunol. 2012;145:44-54 pubmed publisher
	Abreu M, Sealy L. Cells expressing the C/EBPbeta isoform, LIP, engulf their neighbors. PLoS ONE. 2012;7:e41807 pubmed publisher
	Grimbert P, Bouguermouh S, Baba N, Nakajima T, Allakhverdi Z, Braun D, et al. Thrombospondin/CD47 interaction: a pathway to generate regulatory T cells from human CD4+ CD25- T cells in response to inflammation. J Immunol. 2006;177:3534-41 pubmed

product information

Product Type :

Antibody

Product Name :

CD47 Monoclonal Antibody (B6H12), eBioscience™

Catalog # :

14-0479-80

Quantity :

25 µg

Price :

US 309.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: 1 µg/test, Immunohistochemistry (Paraffin): Assay-Dependent, Immunoprecipitation: Assay-Dependent, Western Blot: Assay-Dependent

Species :

Human

Clone :

B6H12

Isotype :

IgG1, kappa

Storage :

4° C

Description :

CD47 (integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Functionally, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. CD47 is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. CD47 has broad tissue distribution, is reduced in expression on Rh erythrocytes. CD47 acts as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion, T cell activation, and is involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1 µg/test, Immunohistochemistry (Paraffin): Assay-Dependent, Immunoprecipitation: Assay-Dependent, Western Blot: Assay-Dependent

Aliases :

9130415E20Rik; AA407862; AI848868; antigen identified by monoclonal antibody 1D8; antigenic surface determinant protein OA3; AW108519; B430305P08Rik; Cd47; CD47 antigen; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 antigen/integrin-associated protein; CD47 glycoprotein; CD47 molecule; CD47/IAP; IAP; integrin associated protein; integrin-associated protein; integrin-associated signal transducer; Itgp; Leukocyte surface antigen CD47; MER6; OA3; Protein MER6; Rh-related antigen; sCD47; soluble CD 47; soluble CD47

more info or order :

Invitrogen product webpage