CD47 Monoclonal Antibody (2D3), eBioscience™ | Invitrogen 14-0478-82 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD47 Monoclonal Antibody (2D3), eBioscience™

catalog :

14-0478-82

quantity :

100 µg

price :

US 309.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2D3

reactivity :

human

application :

western blot, flow cytometry, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
blocking or activating experiments; human; 10 ug/ml; fig 5b	Reed M, Luissint A, Azcutia V, Fan S, O Leary M, Quirós M, et al. Epithelial CD47 is critical for mucosal repair in the murine intestine in vivo. Nat Commun. 2019;10:5004 pubmed publisher
western blot; human	Martínez Torres A, Quiney C, Attout T, Boullet H, Herbi L, Vela L, et al. CD47 agonist peptides induce programmed cell death in refractory chronic lymphocytic leukemia B cells via PLCÎ³1 activation: evidence from mice and humans. PLoS Med. 2015;12:e1001796 pubmed publisher
	Yoon D, Yoon D, Sim H, Hwang I, Lee J, Chun W. Accelerated Wound Healing by Fibroblasts Differentiated from Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells in a Pressure Ulcer Animal Model. Stem Cells Int. 2018;2018:4789568 pubmed publisher
	Toledano N, Gur Wahnon D, Ben Yehuda A, Rachmilewitz J. Novel CD47: SIRP? dependent mechanism for the activation of STAT3 in antigen-presenting cell. PLoS ONE. 2013;8:e75595 pubmed publisher
	Filla M, Schwinn M, Sheibani N, Kaufman P, Peters D. Regulation of cross-linked actin network (CLAN) formation in human trabecular meshwork (HTM) cells by convergence of distinct beta1 and beta3 integrin pathways. Invest Ophthalmol Vis Sci. 2009;50:5723-31 pubmed publisher
	Subramanian S, Parthasarathy R, Sen S, Boder E, Discher D. Species- and cell type-specific interactions between CD47 and human SIRPalpha. Blood. 2006;107:2548-56 pubmed

product information

Product Type :

Antibody

Product Name :

CD47 Monoclonal Antibody (2D3), eBioscience™

Catalog # :

14-0478-82

Quantity :

100 µg

Price :

US 309.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: 0.5 µg/test, Western Blot: Assay-Dependent

Species :

Human

Clone :

2D3

Isotype :

IgG1, kappa

Storage :

4° C

Description :

CD47 (integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Functionally, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. CD47 is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. CD47 has broad tissue distribution, is reduced in expression on Rh erythrocytes. CD47 acts as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion, T cell activation, and is involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.5 µg/test, Western Blot: Assay-Dependent

Aliases :

9130415E20Rik; AA407862; AI848868; antigen identified by monoclonal antibody 1D8; antigenic surface determinant protein OA3; AW108519; B430305P08Rik; Cd47; CD47 antigen; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 antigen/integrin-associated protein; CD47 glycoprotein; CD47 molecule; CD47/IAP; IAP; integrin associated protein; integrin-associated protein; integrin-associated signal transducer; Itgp; Leukocyte surface antigen CD47; MER6; OA3; Protein MER6; Rh-related antigen; sCD47; soluble CD 47; soluble CD47

more info or order :

Invitrogen product webpage