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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD45 Monoclonal Antibody (HI30), eBioscience
catalog :
14-0459-37
quantity :
2 mg
price :
US 1378.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
HI30
reactivity :
human, mouse
application :
immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section, mass cytometry
more info or order :
citations: 74
Published Application/Species/Sample/DilutionReference
  • mass cytometry; human; fig 2h
Wang Z, He L, Li W, Xu C, Zhang J, Wang D, et al. GDF15 induces immunosuppression via CD48 on regulatory T cells in hepatocellular carcinoma. J Immunother Cancer. 2021;9: pubmed publisher
  • flow cytometry; human; 1:50; loading ...
Turner J, Kim W, Kalaidina E, Goss C, Rauseo A, Schmitz A, et al. SARS-CoV-2 infection induces long-lived bone marrow plasma cells in humans. Nature. 2021;595:421-425 pubmed publisher
  • flow cytometry; human
Laurent E, Sieber A, Salzer B, Wachernig A, Seigner J, Lehner M, et al. Directed Evolution of Stabilized Monomeric CD19 for Monovalent CAR Interaction Studies and Monitoring of CAR-T Cell Patients. ACS Synth Biol. 2021;10:1184-1198 pubmed publisher
  • flow cytometry; human; loading ...; fig 2b
Liu G, Yu Y, Feng F, Zhu P, Zhang H, Zhang D, et al. Human CD8+CD28- T suppressor cells expanded by common gamma chain (γc) cytokines retain steady allospecific suppressive capacity in vivo. BMC Immunol. 2020;21:23 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3h, 6e
Lynn R, Weber E, Sotillo E, Gennert D, Xu P, Good Z, et al. c-Jun overexpression in CAR T cells induces exhaustion resistance. Nature. 2019;576:293-300 pubmed publisher
Deng H, Lei Q, Wang C, Wang Z, Chen H, Wang G, et al. A fluorogenic probe for predicting treatment response in non-small cell lung cancer with EGFR-activating mutations. Nat Commun. 2022;13:6944 pubmed publisher
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Narunsky Haziza L, Sepich Poore G, Livyatan I, Asraf O, Martino C, Nejman D, et al. Pan-cancer analyses reveal cancer-type-specific fungal ecologies and bacteriome interactions. Cell. 2022;185:3789-3806.e17 pubmed publisher
Liu Y, Zhang X, Hu Y, Kang M, Wu Y, Wang Y, et al. Human placental mesenchymal stem cells regulate inflammation via the NF‑κB signaling pathway. Exp Ther Med. 2022;24:654 pubmed publisher
Wang Y, Kong L, Sun B, Cui J, Shen W. Celecoxib induces adipogenic differentiation of hemangioma-derived mesenchymal stem cells through the PPAR-γ pathway in vitro and in vivo. Exp Ther Med. 2022;23:375 pubmed publisher
Johnson B, Creason A, Stommel J, Keck J, Parmar S, Betts C, et al. An omic and multidimensional spatial atlas from serial biopsies of an evolving metastatic breast cancer. Cell Rep Med. 2022;3:100525 pubmed publisher
Scherer S, Riggio A, Haroun F, Derose Y, Ekiz H, Fujita M, et al. An immune-humanized patient-derived xenograft model of estrogen-independent, hormone receptor positive metastatic breast cancer. Breast Cancer Res. 2021;23:100 pubmed publisher
Kozuka M, Battaglin F, Jayachandran P, Wang J, Arai H, Soni S, et al. Clinical Significance of Circulating Tumor Cell Induced Epithelial-Mesenchymal Transition in Patients with Metastatic Colorectal Cancer by Single-Cell RNA-Sequencing. Cancers (Basel). 2021;13: pubmed publisher
Suryawanshi G, Arokium H, Kim S, Khamaikawin W, Lin S, Shimizu S, et al. Longitudinal clonal tracking in humanized mice reveals sustained polyclonal repopulation of gene-modified human-HSPC despite vector integration bias. Stem Cell Res Ther. 2021;12:528 pubmed publisher
Ferr xe9 E, Schmitt M, Ochoa S, Rosen L, Shaw E, Burbelo P, et al. SARS-CoV-2 Spike Protein-Directed Monoclonal Antibodies May Ameliorate COVID-19 Complications in APECED Patients. Front Immunol. 2021;12:720205 pubmed publisher
Chen C, Huang X, Zhu W, Ding C, Huang P, Li R. TRPA1 triggers hyperalgesia and inflammation after tooth bleaching. Sci Rep. 2021;11:17418 pubmed publisher
Schreurs R, Baumdick M, Drewniak A, Bunders M. In vitro co-culture of human intestinal organoids and lamina propria-derived CD4+ T cells. STAR Protoc. 2021;2:100519 pubmed publisher
Tichy E, Ma N, Sidibe D, Loro E, Kocan J, Chen D, et al. Persistent NF-κB activation in muscle stem cells induces proliferation-independent telomere shortening. Cell Rep. 2021;35:109098 pubmed publisher
YEKU O, Rao T, Laster I, Kononenko A, Purdon T, Wang P, et al. Bispecific T-Cell Engaging Antibodies Against MUC16 Demonstrate Efficacy Against Ovarian Cancer in Monotherapy and in Combination With PD-1 and VEGF Inhibition. Front Immunol. 2021;12:663379 pubmed publisher
Pfister D, N xfa xf1 ez N, Pinyol R, Govaere O, Pinter M, Szydlowska M, et al. NASH limits anti-tumour surveillance in immunotherapy-treated HCC. Nature. 2021;592:450-456 pubmed publisher
Wang X, He Y, Zhang Q, Ren X, Zhang Z. Direct Comparative Analyses of 10X Genomics Chromium and Smart-seq2. Genomics Proteomics Bioinformatics. 2021;19:253-266 pubmed publisher
Lei H, Xu H, Shan H, Liu M, Lu Y, Fang Z, et al. Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia. Nat Commun. 2021;12:51 pubmed publisher
Parayath N, Stephan S, Koehne A, Nelson P, Stephan M. In vitro-transcribed antigen receptor mRNA nanocarriers for transient expression in circulating T cells in vivo. Nat Commun. 2020;11:6080 pubmed publisher
Tamminga M, Oomens L, Hiltermann T, Andree K, Tibbe A, Broekmaat J, et al. Microsieves for the detection of circulating tumor cells in leukapheresis product in non-small cell lung cancer patients. Transl Lung Cancer Res. 2020;9:1093-1100 pubmed publisher
Moreau G, Burgess S, Sturek J, Donlan A, Petri W, Mann B. Evaluation of K18-hACE2 Mice as a Model of SARS-CoV-2 Infection. Am J Trop Med Hyg. 2020;103:1215-1219 pubmed publisher
Li X, Zhang B, Wang H, Zhao X, Zhang Z, Ding G, et al. Aging affects responsiveness of peripheral blood mononuclear cells to immunosuppression of periodontal ligament stem cells. J Int Med Res. 2020;48:300060520930853 pubmed publisher
Soldi R, Ghosh Halder T, Weston A, Thode T, Drenner K, Lewis R, et al. The novel reversible LSD1 inhibitor SP-2577 promotes anti-tumor immunity in SWItch/Sucrose-NonFermentable (SWI/SNF) complex mutated ovarian cancer. PLoS ONE. 2020;15:e0235705 pubmed publisher
Hu J, Wang Z, Chen Z, Li A, Sun J, Zheng M, et al. DKK2 blockage-mediated immunotherapy enhances anti-angiogenic therapy of Kras mutated colorectal cancer. Biomed Pharmacother. 2020;127:110229 pubmed publisher
Huang J, Wang Y, Lo S, Tseng L, Chen D, Wu Y, et al. Visfatin Mediates Malignant Behaviors through Adipose-Derived Stem Cells Intermediary in Breast Cancer. Cancers (Basel). 2019;12: pubmed publisher
Lai A, Chi P, Thio C, Han Y, Kao H, Hsieh H, et al. α-Lactosylceramide Protects Against iNKT-Mediated Murine Airway Hyperreactivity and Liver Injury Through Competitive Inhibition of Cd1d Binding. Front Chem. 2019;7:811 pubmed publisher
Ewald L, Dittmann J, Vogler M, Fulda S. Side-by-side comparison of BH3-mimetics identifies MCL-1 as a key therapeutic target in AML. Cell Death Dis. 2019;10:917 pubmed publisher
Ladinsky M, Khamaikawin W, Jung Y, Lin S, Lam J, An D, et al. Mechanisms of virus dissemination in bone marrow of HIV-1-infected humanized BLT mice. elife. 2019;8: pubmed publisher
Zeng R, Zheng Y, Fan R, Zhou G, Zhang Y, Mai S, et al. Si-ni-tang (a Chinese herbal formula) for improving immunofunction in sepsis: study protocol for a pilot randomized controlled trial. Trials. 2019;20:537 pubmed publisher
Yu B, Li Q, Zhou M. LPS‑induced upregulation of the TLR4 signaling pathway inhibits osteogenic differentiation of human periodontal ligament stem cells under inflammatory conditions. Int J Mol Med. 2019;43:2341-2351 pubmed publisher
Yan N, Xu J, Zhao C, Wu Y, Gao F, Li C, et al. Human umbilical cord-derived mesenchymal stem cells ameliorate the enteropathy of food allergies in mice. Exp Ther Med. 2018;16:4445-4456 pubmed publisher
Jankovic Karasoulos T, McAninch D, McCullough D, Wilson R, Bianco Miotto T, Roberts C. Isolation of villous cytotrophoblasts from second trimester human placentas. Placenta. 2018;74:55-58 pubmed publisher
Pievani A, Michelozzi I, Rambaldi B, Granata V, Corsi A, Dazzi F, et al. Fludarabine as a cost-effective adjuvant to enhance engraftment of human normal and malignant hematopoiesis in immunodeficient mice. Sci Rep. 2018;8:9125 pubmed publisher
Nellan A, Rota C, Majzner R, Lester McCully C, Griesinger A, Mulcahy Levy J, et al. Durable regression of Medulloblastoma after regional and intravenous delivery of anti-HER2 chimeric antigen receptor T cells. J Immunother Cancer. 2018;6:30 pubmed publisher
Wang Y, Guo L, Feng L, Zhang W, Xiao T, Di X, et al. Single nucleotide variant profiles of viable single circulating tumour cells reveal CTC behaviours in breast cancer. Oncol Rep. 2018;39:2147-2159 pubmed publisher
Xiao Q, Wu J, Wang W, Chen S, Zheng Y, Yu X, et al. DKK2 imparts tumor immunity evasion through β-catenin-independent suppression of cytotoxic immune-cell activation. Nat Med. 2018;24:262-270 pubmed publisher
Apostolou P, Ntanovasilis D, Papasotiriou I. Evaluation of a simple method for storage of blood samples that enables isolation of circulating tumor cells 96 h after sample collection. J Biol Res (Thessalon). 2017;24:11 pubmed publisher
Igarashi A, Ebihara Y, Kumagai T, Hirai H, Nagata K, Tsuji K. Mast cells derived from human induced pluripotent stem cells are useful for allergen tests. Allergol Int. 2018;67:234-242 pubmed publisher
Li Y, Brauer P, Singh J, Xhiku S, Yoganathan K, Zuniga Pflucker J, et al. Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis. Stem Cell Reports. 2017;9:779-795 pubmed publisher
Lee R, Reese C, Carmen Lopez G, Perry B, Bonner M, Zemskova M, et al. Deficient Adipogenesis of Scleroderma Patient and Healthy African American Monocytes. Front Pharmacol. 2017;8:174 pubmed publisher
Syed Khaja A, Toor S, El Salhat H, Faour I, Ul Haq N, Ali B, et al. Preferential accumulation of regulatory T cells with highly immunosuppressive characteristics in breast tumor microenvironment. Oncotarget. 2017;8:33159-33171 pubmed publisher
Li J, Mao Q, He J, She H, Zhang Z, Yin C. Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism. Stem Cell Res Ther. 2017;8:55 pubmed publisher
Boardman D, Philippeos C, Fruhwirth G, Ibrahim M, Hannen R, Cooper D, et al. Expression of a Chimeric Antigen Receptor Specific for Donor HLA Class I Enhances the Potency of Human Regulatory T Cells in Preventing Human Skin Transplant Rejection. Am J Transplant. 2017;17:931-943 pubmed publisher
Liu Z, Guo W, Zhang D, Pang Y, Shi J, Wan S, et al. Circulating tumor cell detection in hepatocellular carcinoma based on karyoplasmic ratios using imaging flow cytometry. Sci Rep. 2016;6:39808 pubmed publisher
Dowling J, Cai Y, Bertin J, Gough P, Zhang J. Kinase-independent function of RIP1, critical for mature T-cell survival and proliferation. Cell Death Dis. 2016;7:e2379 pubmed publisher
Andrews F, Tong Q, Sullivan K, Cornett E, Zhang Y, Ali M, et al. Multivalent Chromatin Engagement and Inter-domain Crosstalk Regulate MORC3 ATPase. Cell Rep. 2016;16:3195-3207 pubmed publisher
Jin Y, Zhou J, Xu F, Jin B, Cui L, Wang Y, et al. Targeting methyltransferase PRMT5 eliminates leukemia stem cells in chronic myelogenous leukemia. J Clin Invest. 2016;126:3961-3980 pubmed publisher
Mitchell A, Gray W, Hayek S, Ko Y, Thomas S, Rooney K, et al. Platelets confound the measurement of extracellular miRNA in archived plasma. Sci Rep. 2016;6:32651 pubmed publisher
Benyoucef A, Palii C, Wang C, Porter C, Chu A, Dai F, et al. UTX inhibition as selective epigenetic therapy against TAL1-driven T-cell acute lymphoblastic leukemia. Genes Dev. 2016;30:508-21 pubmed publisher
González S, Mei H, Nakatsu M, Baclagon E, Deng S. A 3D culture system enhances the ability of human bone marrow stromal cells to support the growth of limbal stem/progenitor cells. Stem Cell Res. 2016;16:358-64 pubmed publisher
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Gargus M, Niu C, Vallone J, Binkley J, Rubin D, Shaker A. Human esophageal myofibroblasts secrete proinflammatory cytokines in response to acid and Toll-like receptor 4 ligands. Am J Physiol Gastrointest Liver Physiol. 2015;308:G904-23 pubmed
Dowling J, Nair A, Zhang J. A novel function of RIP1 in postnatal development and immune homeostasis by protecting against RIP3-dependent necroptosis and FADD-mediated apoptosis. Front Cell Dev Biol. 2015;3:12 pubmed publisher
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product information
Product Type :
Antibody
Product Name :
CD45 Monoclonal Antibody (HI30), eBioscience
Catalog # :
14-0459-37
Quantity :
2 mg
Price :
US 1378.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Human
Applications :
Flow Cytometry: 0.25 µg/test, Immunohistochemistry (Paraffin): 1:100, Immunohistochemistry: Assay-Dependent
Species :
Human
Clone :
HI30
Isotype :
IgG1, kappa
Storage :
4° C
Description :
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.25 µg/test, Immunohistochemistry (Paraffin): 1:100, Immunohistochemistry: Assay-Dependent
Aliases :
B220; cd45; CD45 antigen; CD45 antigen isoform 1 precursor; CD45 antigen isoform 2 precursor; CD45 antigen isoform 3 precursor; CD45 antigen isoform 4 precursor; CD45 antigen isoform 5 precursor; CD45 antigen isoform 6 precursor; CD45R; GP180; Lca; L-CA; leucocyte common antigen; leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; leukocyte common antigen, CD45; loc; LOW QUALITY PROTEIN: receptor-type tyrosine-protein phosphatase C; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; membrane tyrosine phosphatase; protein tyrosine phosphatase lambda; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; protein tyrosine phosphatase; alternatively spliced; Ptprc; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA