CD45RA Monoclonal Antibody (HI100), eBioscience | Invitrogen 14-0458-37 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD45RA Monoclonal Antibody (HI100), eBioscience

catalog :

14-0458-37

quantity :

2 mg

price :

US 1372.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

HI100

reactivity :

human

application :

immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 32

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 2e	Pasciuto E, Burton O, Roca C, Lagou V, Rajan W, Theys T, et al. Microglia Require CD4 T Cells to Complete the Fetal-to-Adult Transition. Cell. 2020;182:625-640.e24 pubmed publisher
	Jiang B, Wang S, Song G, Jiang Q, Fan M, Fang C, et al. Hedgehog-induced ZFYVE21 promotes chronic vascular inflammation by activating NLRP3 inflammasomes in T cells. Sci Signal. 2023;16:eabo3406 pubmed publisher
	Qian Y, Arellano G, Ifergan I, Lin J, Snowden C, Kim T, et al. ZEB1 promotes pathogenic Th1 and Th17 cell differentiation in multiple sclerosis. Cell Rep. 2021;36:109602 pubmed publisher
	Zhang Y, Zhang J, Shi Y, Shen M, Lv H, Chen S, et al. Differences in Maturation Status and Immune Phenotypes of Circulating Helios+ and Helios- Tregs and Their Disrupted Correlations With Monocyte Subsets in Autoantibody-Positive T1D Individuals. Front Immunol. 2021;12:628504 pubmed publisher
	Louka E, Povinelli B, Rodriguez Meira A, Buck G, Wen W, Wang G, et al. Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia. J Exp Med. 2021;218: pubmed publisher
	Bauman J, Ohr J, Gooding W, Ferris R, Duvvuri U, Kim S, et al. Phase I Study of Ficlatuzumab and Cetuximab in Cetuximab-Resistant, Recurrent/Metastatic Head and Neck Cancer. Cancers (Basel). 2020;12: pubmed publisher
	Psaila B, Wang G, Rodriguez Meira A, Li R, Heuston E, Murphy L, et al. Single-Cell Analyses Reveal Megakaryocyte-Biased Hematopoiesis in Myelofibrosis and Identify Mutant Clone-Specific Targets. Mol Cell. 2020;78:477-492.e8 pubmed publisher
	Mesa Nuñez C, León Rico D, Aldea M, Damián C, Sanchez Baltasar R, Sánchez R, et al. The downregulated membrane expression of CD18 in CD34+ cells defines a primitive population of human hematopoietic stem cells. Stem Cell Res Ther. 2020;11:164 pubmed publisher
	Bekeschus S, Ressel V, Freund E, Gelbrich N, Mustea A, Stope M. Gas Plasma-Treated Prostate Cancer Cells Augment Myeloid Cell Activity and Cytotoxicity. Antioxidants (Basel). 2020;9: pubmed publisher
	Lv M, Li Z, Liu J, Lin F, Zhang Q, Li Z, et al. MicroRNA‑155 inhibits the proliferation of CD8+ T cells via upregulating regulatory T cells in vitiligo. Mol Med Rep. 2019;20:3617-3624 pubmed publisher
	Bell C, Fennell K, Chan Y, Rambow F, Yeung M, Vassiliadis D, et al. Targeting enhancer switching overcomes non-genetic drug resistance in acute myeloid leukaemia. Nat Commun. 2019;10:2723 pubmed publisher
	Jarrell J, Twite A, Lau K, Kashani M, Lievano A, Acevedo J, et al. Intracellular delivery of mRNA to human primary T cells with microfluidic vortex shedding. Sci Rep. 2019;9:3214 pubmed publisher
	Muller Durovic B, Grählert J, Devine O, Akbar A, Hess C. CD56-negative NK cells with impaired effector function expand in CMV and EBV co-infected healthy donors with age. Aging (Albany NY). 2019;11:724-740 pubmed publisher
	Schlenner S, Pasciuto E, Lagou V, Burton O, Prezzemolo T, Junius S, et al. NFIL3 mutations alter immune homeostasis and sensitise for arthritis pathology. Ann Rheum Dis. 2019;78:342-349 pubmed publisher
	Catakovic K, Gassner F, Ratswohl C, Zaborsky N, Rebhandl S, Schubert M, et al. TIGIT expressing CD4+T cells represent a tumor-supportive T cell subset in chronic lymphocytic leukemia. Oncoimmunology. 2017;7:e1371399 pubmed publisher
	Sanderson S, Simon A. In aged primary T cells, mitochondrial stress contributes to telomere attrition measured by a novel imaging flow cytometry assay. Aging Cell. 2017;16:1234-1243 pubmed publisher
	Eyquem J, Mansilla Soto J, Giavridis T, van der Stegen S, Hamieh M, Cunanan K, et al. Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection. Nature. 2017;543:113-117 pubmed publisher
	Sheng S, Gu Y, Lu C, Zou J, Hong H, Wang R. The distribution and function of human memory T cell subsets in lung cancer. Immunol Res. 2017;65:639-650 pubmed publisher
	Abrahimi P, Chang W, Kluger M, Qyang Y, Tellides G, Saltzman W, et al. Efficient gene disruption in cultured primary human endothelial cells by CRISPR/Cas9. Circ Res. 2015;117:121-8 pubmed publisher
	Van Eyck L, De Somer L, Pombal D, Bornschein S, Frans G, Humblet Baron S, et al. Brief Report: IFIH1 Mutation Causes Systemic Lupus Erythematosus With Selective IgA Deficiency. Arthritis Rheumatol. 2015;67:1592-7 pubmed publisher
	Komori H, Hart T, Lamere S, Chew P, Salomon D. Defining CD4 T cell memory by the epigenetic landscape of CpG DNA methylation. J Immunol. 2015;194:1565-79 pubmed publisher
	Schubert D, Bode C, Kenefeck R, Hou T, Wing J, Kennedy A, et al. Autosomal dominant immune dysregulation syndrome in humans with CTLA4 mutations. Nat Med. 2014;20:1410-1416 pubmed publisher
	Davey M, Morgan M, Liuzzi A, Tyler C, Khan M, Szakmany T, et al. Microbe-specific unconventional T cells induce human neutrophil differentiation into antigen cross-presenting cells. J Immunol. 2014;193:3704-3716 pubmed publisher
	Wu X, Zhang H, Xing Q, Cui J, Li J, Li Y, et al. PD-1(+) CD8(+) T cells are exhausted in tumours and functional in draining lymph nodes of colorectal cancer patients. Br J Cancer. 2014;111:1391-9 pubmed publisher
	Himmel M, Macdonald K, Garcia R, Steiner T, Levings M. Helios+ and Helios- cells coexist within the natural FOXP3+ T regulatory cell subset in humans. J Immunol. 2013;190:2001-8 pubmed publisher
	Welton J, Morgan M, Marti S, Stone M, Moser B, Sewell A, et al. Monocytes and ?? T cells control the acute-phase response to intravenous zoledronate: insights from a phase IV safety trial. J Bone Miner Res. 2013;28:464-71 pubmed publisher
	Stemberger C, Dreher S, Tschulik C, Piossek C, Bet J, Yamamoto T, et al. Novel serial positive enrichment technology enables clinical multiparameter cell sorting. PLoS ONE. 2012;7:e35798 pubmed publisher
	Fogal B, Yi T, Wang C, Rao D, Lebastchi A, Kulkarni S, et al. Neutralizing IL-6 reduces human arterial allograft rejection by allowing emergence of CD161+ CD4+ regulatory T cells. J Immunol. 2011;187:6268-80 pubmed publisher
	Huntington N, Alves N, Legrand N, Lim A, Strick Marchand H, Mention J, et al. IL-15 transpresentation promotes both human T-cell reconstitution and T-cell-dependent antibody responses in vivo. Proc Natl Acad Sci U S A. 2011;108:6217-22 pubmed publisher
	Walker J, Maier C, Pober J. Cytomegalovirus-infected human endothelial cells can stimulate allogeneic CD4+ memory T cells by releasing antigenic exosomes. J Immunol. 2009;182:1548-59 pubmed
	Huarte E, Cubillos Ruiz J, Nesbeth Y, Scarlett U, Martinez D, Engle X, et al. PILAR is a novel modulator of human T-cell expansion. Blood. 2008;112:1259-68 pubmed publisher
	Arimura Y, Vang T, Tautz L, Williams S, Mustelin T. TCR-induced downregulation of protein tyrosine phosphatase PEST augments secondary T cell responses. Mol Immunol. 2008;45:3074-84 pubmed publisher

product information

Product Type :

Antibody

Product Name :

CD45RA Monoclonal Antibody (HI100), eBioscience

Catalog # :

14-0458-37

Quantity :

2 mg

Price :

US 1372.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: 0.5 µg/test, Immunohistochemistry (Paraffin): 5 µg/mL

Species :

Human

Clone :

HI100

Isotype :

IgG2b, kappa

Storage :

4° C

Description :

CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing and these isoforms differ in their extracellular domains, however, they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage, with the exception of mature red cells and is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Diseases associated with CD45 dysfunction include severe combined immunodeficiency and Hepatitis C.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.5 µg/test, Immunohistochemistry (Paraffin): 5 µg/mL

Aliases :

B220; CD45; CD45 antigen; CD45 antigen isoform 1 precursor; CD45 antigen isoform 2 precursor; CD45 antigen isoform 3 precursor; CD45 antigen isoform 4 precursor; CD45 antigen isoform 5 precursor; CD45 antigen isoform 6 precursor; CD45R; GP180; LCA; L-CA; leucocyte common antigen; Leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; LOW QUALITY PROTEIN: receptor-type tyrosine-protein phosphatase C; LY5; Ly-5; Lyt-4; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; protein tyrosine phosphatase; alternatively spliced; PTPRC; Receptor-type tyrosine-protein phosphatase C; RT7; T100; T200; T200 glycoprotein; T200 leukocyte common antigen

more info or order :

Invitrogen product webpage