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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD27 Monoclonal Antibody (LG.7F9), eBioscience™
catalog :
14-0271-82
quantity :
100µg
price :
US 103
clonality :
monoclonal
host :
hamsters
conjugate :
nonconjugated
clone name :
LG.7F9
reactivity :
human, mouse, rat
application :
neutralization, immunoprecipitation, flow cytometry
more info or order :
citations: 70
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig 3g
Moffett H, Harms C, Fitzpatrick K, Tooley M, Boonyaratanakornkit J, Taylor J. B cells engineered to express pathogen-specific antibodies protect against infection. Sci Immunol. 2019;4: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Karmaus P, Chen X, Lim S, Herrada A, Nguyen T, Xu B, et al. Metabolic heterogeneity underlies reciprocal fates of TH17 cell stemness and plasticity. Nature. 2019;565:101-105 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3a
Jensen I, Winborn C, Fosdick M, Shao P, Tremblay M, Shan Q, et al. Polymicrobial sepsis influences NK-cell-mediated immunity by diminishing NK-cell-intrinsic receptor-mediated effector responses to viral ligands or infections. PLoS Pathog. 2018;14:e1007405 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3i
Geary C, Krishna C, Lau C, Adams N, Gearty S, Pritykin Y, et al. Non-redundant ISGF3 Components Promote NK Cell Survival in an Auto-regulatory Manner during Viral Infection. Cell Rep. 2018;24:1949-1957.e6 pubmed publisher
  • flow cytometry; mouse; 1:300; loading ...; fig s7b
Zhu L, Xie X, Zhang L, Wang H, Jie Z, Zhou X, et al. TBK-binding protein 1 regulates IL-15-induced autophagy and NKT cell survival. Nat Commun. 2018;9:2812 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1e
Abel A, Tiwari A, Gerbec Z, Siebert J, Yang C, Schloemer N, et al. IQ Domain-Containing GTPase-Activating Protein 1 Regulates Cytoskeletal Reorganization and Facilitates NKG2D-Mediated Mechanistic Target of Rapamycin Complex 1 Activation and Cytokine Gene Translation in Natural Killer Cells. Front Immunol. 2018;9:1168 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Omilusik K, Nadjsombati M, Shaw L, Yu B, Milner J, Goldrath A. Sustained Id2 regulation of E proteins is required for terminal differentiation of effector CD8+ T cells. J Exp Med. 2018;215:773-783 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1d
Ibitokou S, Dillon B, Sinha M, Szczesny B, Delgadillo A, Reda Abdelrahman D, et al. Early Inhibition of Fatty Acid Synthesis Reduces Generation of Memory Precursor Effector T Cells in Chronic Infection. J Immunol. 2018;200:643-656 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3d
Bern M, Beckman D, Ebihara T, Taffner S, Poursine Laurent J, White J, et al. Immunoreceptor tyrosine-based inhibitory motif-dependent functions of an MHC class I-specific NK cell receptor. Proc Natl Acad Sci U S A. 2017;114:E8440-E8447 pubmed publisher
  • flow cytometry; mouse; fig s6b
Danahy D, Anthony S, Jensen I, Hartwig S, Shan Q, Xue H, et al. Polymicrobial sepsis impairs bystander recruitment of effector cells to infected skin despite optimal sensing and alarming function of skin resident memory CD8 T cells. PLoS Pathog. 2017;13:e1006569 pubmed publisher
  • flow cytometry; mouse; fig s1b
Knudson K, Pritzl C, Saxena V, Altman A, Daniels M, Teixeiro E. NFκB-Pim-1-Eomesodermin axis is critical for maintaining CD8 T-cell memory quality. Proc Natl Acad Sci U S A. 2017;114:E1659-E1667 pubmed publisher
  • flow cytometry; mouse; loading ...
Marshall N, Vong A, Devarajan P, Brauner M, Kuang Y, Nayar R, et al. NKG2C/E Marks the Unique Cytotoxic CD4 T Cell Subset, ThCTL, Generated by Influenza Infection. J Immunol. 2017;198:1142-1155 pubmed publisher
  • flow cytometry; human; fig 2a
Ribeiro S, Tesio M, Ribot J, Macintyre E, Barata J, Silva Santos B. Casein kinase 2 controls the survival of normal thymic and leukemic ?? T cells via promotion of AKT signaling. Leukemia. 2017;31:1603-1610 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Hoegl S, Ehrentraut H, Brodsky K, Victorino F, Golden Mason L, Eltzschig H, et al. NK cells regulate CXCR2+ neutrophil recruitment during acute lung injury. J Leukoc Biol. 2017;101:471-480 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1f, 1g
Guo H, Cranert S, Lu Y, Zhong M, Zhang S, Chen J, et al. Deletion of Slam locus in mice reveals inhibitory role of SLAM family in NK cell responses regulated by cytokines and LFA-1. J Exp Med. 2016;213:2187-207 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4h
Drennan M, Govindarajan S, Verheugen E, Coquet J, Staal J, McGuire C, et al. NKT sublineage specification and survival requires the ubiquitin-modifying enzyme TNFAIP3/A20. J Exp Med. 2016;213:1973-81 pubmed publisher
  • flow cytometry; mouse; 1:200; fig s4
Ludigs K, Jandus C, Utzschneider D, Staehli F, Bessoles S, Dang A, et al. NLRC5 shields T lymphocytes from NK-cell-mediated elimination under inflammatory conditions. Nat Commun. 2016;7:10554 pubmed publisher
  • flow cytometry; mouse
Lasigliè D, Boero S, Bauer I, Morando S, Damonte P, Cea M, et al. Sirt6 regulates dendritic cell differentiation, maturation, and function. Aging (Albany NY). 2016;8:34-49 pubmed
  • flow cytometry; mouse; fig 3
Zhang Z, Wu N, Lu Y, Davidson D, Colonna M, Veillette A. DNAM-1 controls NK cell activation via an ITT-like motif. J Exp Med. 2015;212:2165-82 pubmed publisher
  • flow cytometry; human; 10 ug/ml
Leong M, Newell E. Multiplexed Peptide-MHC Tetramer Staining with Mass Cytometry. Methods Mol Biol. 2015;1346:115-31 pubmed publisher
  • flow cytometry; mouse
Pearce V, Bouabe H, MacQueen A, Carbonaro V, Okkenhaug K. PI3Kδ Regulates the Magnitude of CD8+ T Cell Responses after Challenge with Listeria monocytogenes. J Immunol. 2015;195:3206-17 pubmed publisher
  • flow cytometry; mouse; fig 5
Wensveen F, Jelenčić V, Valentić S, Šestan M, Wensveen T, Theurich S, et al. NK cells link obesity-induced adipose stress to inflammation and insulin resistance. Nat Immunol. 2015;16:376-85 pubmed publisher
  • flow cytometry; human; fig 1
Severson J, Serracino H, Mateescu V, Raeburn C, McIntyre R, Sams S, et al. PD-1+Tim-3+ CD8+ T Lymphocytes Display Varied Degrees of Functional Exhaustion in Patients with Regionally Metastatic Differentiated Thyroid Cancer. Cancer Immunol Res. 2015;3:620-30 pubmed publisher
  • flow cytometry; mouse; fig 6
Sell S, Dietz M, Schneider A, Holtappels R, Mach M, Winkler T. Control of murine cytomegalovirus infection by γδ T cells. PLoS Pathog. 2015;11:e1004481 pubmed publisher
  • flow cytometry; human; fig 3
de Masson A, Bouaziz J, Le Buanec H, Robin M, O Meara A, Parquet N, et al. CD24(hi)CD27⁺ and plasmablast-like regulatory B cells in human chronic graft-versus-host disease. Blood. 2015;125:1830-9 pubmed publisher
  • flow cytometry; mouse
Krysiak K, Tibbitts J, Shao J, Liu T, Ndonwi M, Walter M. Reduced levels of Hspa9 attenuate Stat5 activation in mouse B cells. Exp Hematol. 2015;43:319-30.e10 pubmed publisher
  • flow cytometry; mouse; fig 3
Buchan S, Manzo T, Flutter B, Rogel A, Edwards N, Zhang L, et al. OX40- and CD27-mediated costimulation synergizes with anti-PD-L1 blockade by forcing exhausted CD8+ T cells to exit quiescence. J Immunol. 2015;194:125-133 pubmed publisher
  • flow cytometry; mouse
Wei H, Nash W, Makrigiannis A, Brown M. Impaired NK-cell education diminishes resistance to murine CMV infection. Eur J Immunol. 2014;44:3273-82 pubmed publisher
  • flow cytometry; human; 1:50
Steinsbø Ø, Henry Dunand C, Huang M, Mesin L, Salgado Ferrer M, Lundin K, et al. Restricted VH/VL usage and limited mutations in gluten-specific IgA of coeliac disease lesion plasma cells. Nat Commun. 2014;5:4041 pubmed publisher
  • flow cytometry; mouse; fig 3
Chopra M, Lang I, Salzmann S, Pachel C, Kraus S, Bäuerlein C, et al. Tumor necrosis factor induces tumor promoting and anti-tumoral effects on pancreatic cancer via TNFR1. PLoS ONE. 2013;8:e75737 pubmed publisher
  • flow cytometry; mouse
Sridhar S, Reyes Sandoval A, Draper S, Moore A, Gilbert S, Gao G, et al. Single-dose protection against Plasmodium berghei by a simian adenovirus vector using a human cytomegalovirus promoter containing intron A. J Virol. 2008;82:3822-33 pubmed publisher
  • flow cytometry; mouse; fig 2
Badovinac V, Harty J. Manipulating the rate of memory CD8+ T cell generation after acute infection. J Immunol. 2007;179:53-63 pubmed
Vink C, Calero Nieto F, Wang X, Maglitto A, Mariani S, Jawaid W, et al. Iterative Single-Cell Analyses Define the Transcriptome of the First Functional Hematopoietic Stem Cells. Cell Rep. 2020;31:107627 pubmed publisher
Ponzetta A, Carriero R, Carnevale S, Barbagallo M, Molgora M, Perucchini C, et al. Neutrophils Driving Unconventional T Cells Mediate Resistance against Murine Sarcomas and Selected Human Tumors. Cell. 2019;178:346-360.e24 pubmed publisher
Simonović N, Witalisz Siepracka A, Meissl K, Lassnig C, Reichart U, Kolbe T, et al. NK Cells Require Cell-Extrinsic and -Intrinsic TYK2 for Full Functionality in Tumor Surveillance and Antibacterial Immunity. J Immunol. 2019;202:1724-1734 pubmed publisher
Bern M, Parikh B, Yang L, Beckman D, Poursine Laurent J, Yokoyama W. Inducible down-regulation of MHC class I results in natural killer cell tolerance. J Exp Med. 2019;216:99-116 pubmed publisher
Cuenca M, Puñet Ortiz J, Ruart M, Terhorst C, Engel P. Ly9 (SLAMF3) receptor differentially regulates iNKT cell development and activation in mice. Eur J Immunol. 2018;48:99-105 pubmed publisher
Chen Z, Stelekati E, Kurachi M, Yu S, Cai Z, Manne S, et al. miR-150 Regulates Memory CD8 T Cell Differentiation via c-Myb. Cell Rep. 2017;20:2584-2597 pubmed publisher
Widjaja C, Olvera J, Metz P, Phan A, Savas J, de Bruin G, et al. Proteasome activity regulates CD8+ T lymphocyte metabolism and fate specification. J Clin Invest. 2017;127:3609-3623 pubmed publisher
Xia P, Liu J, Wang S, Ye B, Du Y, Xiong Z, et al. WASH maintains NKp46+ ILC3 cells by promoting AHR expression. Nat Commun. 2017;8:15685 pubmed publisher
Giltiay N, Shu G, Shock A, Clark E. Targeting CD22 with the monoclonal antibody epratuzumab modulates human B-cell maturation and cytokine production in response to Toll-like receptor 7 (TLR7) and B-cell receptor (BCR) signaling. Arthritis Res Ther. 2017;19:91 pubmed publisher
Filskov J, Mikkelsen M, Hansen P, Christensen J, Thomsen A, Andersen P, et al. Broadening CD4+ and CD8+ T Cell Responses against Hepatitis C Virus by Vaccination with NS3 Overlapping Peptide Panels in Cross-Priming Liposomes. J Virol. 2017;91: pubmed publisher
Ambada G, Ntsama C, Nji N, Ngu L, Sake C, Lissom A, et al. Phenotypic characterization of regulatory T cells from antiretroviral-naive HIV-1-infected people. Immunology. 2017;151:405-416 pubmed publisher
Yang M, Chen S, Du J, He J, Wang Y, Li Z, et al. NK cell development requires Tsc1-dependent negative regulation of IL-15-triggered mTORC1 activation. Nat Commun. 2016;7:12730 pubmed publisher
Reynaldi A, Smith N, Schlub T, Venturi V, Rudd B, Davenport M. Modeling the dynamics of neonatal CD8+ T-cell responses. Immunol Cell Biol. 2016;94:838-848 pubmed publisher
Hamblet C, Makowski S, Tritapoe J, Pomerantz J. NK Cell Maturation and Cytotoxicity Are Controlled by the Intramembrane Aspartyl Protease SPPL3. J Immunol. 2016;196:2614-26 pubmed publisher
Corpuz T, Stolp J, Kim H, Pinget G, Gray D, Cho J, et al. Differential Responsiveness of Innate-like IL-17- and IFN-γ-Producing γδ T Cells to Homeostatic Cytokines. J Immunol. 2016;196:645-54 pubmed publisher
Krneta T, Gillgrass A, Chew M, Ashkar A. The breast tumor microenvironment alters the phenotype and function of natural killer cells. Cell Mol Immunol. 2016;13:628-39 pubmed publisher
Laidlaw B, Cui W, Amezquita R, Gray S, Guan T, Lu Y, et al. Production of IL-10 by CD4(+) regulatory T cells during the resolution of infection promotes the maturation of memory CD8(+) T cells. Nat Immunol. 2015;16:871-9 pubmed publisher
Rei M, Gonçalves Sousa N, Lança T, Thompson R, Mensurado S, Balkwill F, et al. Murine CD27(-) Vγ6(+) γδ T cells producing IL-17A promote ovarian cancer growth via mobilization of protumor small peritoneal macrophages. Proc Natl Acad Sci U S A. 2014;111:E3562-70 pubmed publisher
Forbes C, Scalzo A, Degli Esposti M, Coudert J. Ly49C-dependent control of MCMV Infection by NK cells is cis-regulated by MHC Class I molecules. PLoS Pathog. 2014;10:e1004161 pubmed publisher
Male V, Nisoli I, Kostrzewski T, Allan D, Carlyle J, Lord G, et al. The transcription factor E4bp4/Nfil3 controls commitment to the NK lineage and directly regulates Eomes and Id2 expression. J Exp Med. 2014;211:635-42 pubmed publisher
Mahmud S, Manlove L, Schmitz H, Xing Y, Wang Y, Owen D, et al. Costimulation via the tumor-necrosis factor receptor superfamily couples TCR signal strength to the thymic differentiation of regulatory T cells. Nat Immunol. 2014;15:473-81 pubmed publisher
Knudson K, Goplen N, Cunningham C, Daniels M, Teixeiro E. Low-affinity T cells are programmed to maintain normal primary responses but are impaired in their recall to low-affinity ligands. Cell Rep. 2013;4:554-65 pubmed publisher
Roediger B, Kyle R, Yip K, Sumaria N, Guy T, Kim B, et al. Cutaneous immunosurveillance and regulation of inflammation by group 2 innate lymphoid cells. Nat Immunol. 2013;14:564-73 pubmed publisher
van Helden M, Zaiss D, Sijts A. CCR2 defines a distinct population of NK cells and mediates their migration during influenza virus infection in mice. PLoS ONE. 2012;7:e52027 pubmed publisher
Martin M, Condotta S, Harty J, Badovinac V. Population dynamics of naive and memory CD8 T cell responses after antigen stimulations in vivo. J Immunol. 2012;188:1255-65 pubmed publisher
Rigato P, de Alencar B, de Vasconcelos J, Dominguez M, Araújo A, Machado A, et al. Heterologous plasmid DNA prime-recombinant human adenovirus 5 boost vaccination generates a stable pool of protective long-lived CD8(+) T effector memory cells specific for a human parasite, Trypanosoma cruzi. Infect Immun. 2011;79:2120-30 pubmed publisher
Ribot J, Chaves Ferreira M, d Orey F, Wencker M, Gonçalves Sousa N, Decalf J, et al. Cutting edge: adaptive versus innate receptor signals selectively control the pool sizes of murine IFN-?- or IL-17-producing ?? T cells upon infection. J Immunol. 2010;185:6421-6425 pubmed publisher
Schmidt N, Butler N, Badovinac V, Harty J. Extreme CD8 T cell requirements for anti-malarial liver-stage immunity following immunization with radiation attenuated sporozoites. PLoS Pathog. 2010;6:e1000998 pubmed publisher
Henry T, Kirimanjeswara G, Ruby T, Jones J, Peng K, Perret M, et al. Type I IFN signaling constrains IL-17A/F secretion by gammadelta T cells during bacterial infections. J Immunol. 2010;184:3755-67 pubmed publisher
van Olffen R, Koning N, van Gisbergen K, Wensveen F, Hoek R, Boon L, et al. GITR triggering induces expansion of both effector and regulatory CD4+ T cells in vivo. J Immunol. 2009;182:7490-500 pubmed publisher
Orr S, Quigley L, McVicar D. In vivo expression of signaling proteins in reconstituted NK cells. J Immunol Methods. 2009;340:158-63 pubmed publisher
Hovav A, Panas M, Rahman S, Sircar P, Gillard G, Cayabyab M, et al. Duration of antigen expression in vivo following DNA immunization modifies the magnitude, contraction, and secondary responses of CD8+ T lymphocytes. J Immunol. 2007;179:6725-33 pubmed
Hovav A, Panas M, Osuna C, Cayabyab M, Autissier P, Letvin N. The impact of a boosting immunogen on the differentiation of secondary memory CD8+ T cells. J Virol. 2007;81:12793-802 pubmed
Huntington N, Puthalakath H, Gunn P, Naik E, Michalak E, Smyth M, et al. Interleukin 15-mediated survival of natural killer cells is determined by interactions among Bim, Noxa and Mcl-1. Nat Immunol. 2007;8:856-63 pubmed
Maillard I, Tu L, Sambandam A, Yashiro Ohtani Y, Millholland J, Keeshan K, et al. The requirement for Notch signaling at the beta-selection checkpoint in vivo is absolute and independent of the pre-T cell receptor. J Exp Med. 2006;203:2239-45 pubmed
Tesselaar K, Xiao Y, Arens R, van Schijndel G, Schuurhuis D, Mebius R, et al. Expression of the murine CD27 ligand CD70 in vitro and in vivo. J Immunol. 2003;170:33-40 pubmed
Tesselaar K, Gravestein L, van Schijndel G, Borst J, van Lier R. Characterization of murine CD70, the ligand of the TNF receptor family member CD27. J Immunol. 1997;159:4959-65 pubmed
Gravestein L, Nieland J, Kruisbeek A, Borst J. Novel mAbs reveal potent co-stimulatory activity of murine CD27. Int Immunol. 1995;7:551-7 pubmed
product information
Product Type :
Antibody
Product Name :
CD27 Monoclonal Antibody (LG.7F9), eBioscience™
Catalog # :
14-0271-82
Quantity :
100µg
Price :
US 103
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Armenian Hamster
Reactivity :
Human, Mouse, Rat
Applications :
Flow Cytometry: 0.5 µg/test, Functional assay: Assay-Dependent, Immunoprecipitation: Assay-Dependent, Neutralization: Assay-Dependent
Species :
Human, Mouse, Rat
Clone :
LG.7F9
Isotype :
IgG
Storage :
4° C
Description :
CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.5 µg/test, Functional assay: Assay-Dependent, Immunoprecipitation: Assay-Dependent, Neutralization: Assay-Dependent
Aliases :
CD antigen 27; Cd27; CD27 antigen; CD27 molecule; CD27L receptor; S152; S152. LPFS2; sCD27; soluble CD27; T cell activation antigen S152; T14; T-cell activation antigen CD27; Tnfrsf7; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7; uncharacterized protein LOC100520023
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA