CD21/CD35 Monoclonal Antibody (eBio8D9 (8D9)), eBioscience™ | Invitrogen 14-0211-81 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD21/CD35 Monoclonal Antibody (eBio8D9 (8D9)), eBioscience™

catalog :

14-0211-81

quantity :

50µg

price :

US 74.5

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

eBio8D9 (8D9)

reactivity :

mouse

application :

western blot, flow cytometry

more info or order :

Invitrogen product webpage

citations: 25

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig e10	Majer O, Liu B, Kreuk L, Krogan N, Barton G. UNC93B1 recruits syntenin-1 to dampen TLR7 signalling and prevent autoimmunity. Nature. 2019;575:366-370 pubmed publisher
flow cytometry; mouse; loading ...; fig 3a	Panda S, Facchinetti V, Voynova E, Hanabuchi S, Karnell J, Hanna R, et al. Galectin-9 inhibits TLR7-mediated autoimmunity in murine lupus models. J Clin Invest. 2018;128:1873-1887 pubmed publisher
flow cytometry; mouse; loading ...; fig 5a	van Nieuwenhuijze A, Dooley J, Humblet Baron S, Sreenivasan J, Koenders M, Schlenner S, et al. Defective germinal center B-cell response and reduced arthritic pathology in microRNA-29a-deficient mice. Cell Mol Life Sci. 2017;74:2095-2106 pubmed publisher
flow cytometry; mouse; fig 1	Kolan S, Boman A, Matozaki T, Lejon K, Oldenborg P. Lack of non-hematopoietic SIRPÎ± signaling disturbs the splenic marginal zone architecture resulting in accumulation and displacement of marginal zone B cells. Biochem Biophys Res Commun. 2015;460:645-50 pubmed publisher
flow cytometry; mouse; tbl s3	Pannu J, Belle J, Forster M, Duerr C, Shen S, Kane L, et al. Ubiquitin specific protease 21 is dispensable for normal development, hematopoiesis and lymphocyte differentiation. PLoS ONE. 2015;10:e0117304 pubmed publisher
flow cytometry; mouse	Alsadeq A, Hobeika E, Medgyesi D, Kläsener K, Reth M. The role of the Syk/Shp-1 kinase-phosphatase equilibrium in B cell development and signaling. J Immunol. 2014;193:268-76 pubmed publisher
flow cytometry; mouse; fig s8b	Redecke V, Wu R, Zhou J, Finkelstein D, Chaturvedi V, High A, et al. Hematopoietic progenitor cell lines with myeloid and lymphoid potential. Nat Methods. 2013;10:795-803 pubmed publisher
	Zeng H, Yu M, Tan H, Li Y, Su W, Shi H, et al. Discrete roles and bifurcation of PTEN signaling and mTORC1-mediated anabolic metabolism underlie IL-7-driven B lymphopoiesis. Sci Adv. 2018;4:eaar5701 pubmed publisher
	Niki M, Nakajima K, Ishikawa D, Nishida J, Ishifune C, Tsukumo S, et al. MicroRNA-449a deficiency promotes colon carcinogenesis. Sci Rep. 2017;7:10696 pubmed publisher
	Huang B, Gomez Rodriguez J, Preite S, Garrett L, Harper U, Schwartzberg P. CRISPR-Mediated Triple Knockout of SLAMF1, SLAMF5 and SLAMF6 Supports Positive Signaling Roles in NKT Cell Development. PLoS ONE. 2016;11:e0156072 pubmed publisher
	Jarjour M, Jorquera A, Mondor I, Wienert S, Narang P, Coles M, et al. Fate mapping reveals origin and dynamics of lymph node follicular dendritic cells. J Exp Med. 2014;211:1109-22 pubmed publisher
	Stolp J, Marino E, Batten M, Sierro F, Cox S, Grey S, et al. Intrinsic molecular factors cause aberrant expansion of the splenic marginal zone B cell population in nonobese diabetic mice. J Immunol. 2013;191:97-109 pubmed publisher
	Donius L, Handy J, Weis J, Weis J. Optimal germinal center B cell activation and T-dependent antibody responses require expression of the mouse complement receptor Cr1. J Immunol. 2013;191:434-47 pubmed publisher
	Schneppenheim J, Dressel R, Hüttl S, Lüllmann Rauch R, Engelke M, Dittmann K, et al. The intramembrane protease SPPL2a promotes B cell development and controls endosomal traffic by cleavage of the invariant chain. J Exp Med. 2013;210:41-58 pubmed publisher
	Biswas P, Kang K, Gupta S, Bhagat G, Pernis A. A murine autoimmune model of rheumatoid arthritis and systemic lupus erythematosus associated with deregulated production of IL-17 and IL-21. Methods Mol Biol. 2012;900:233-51 pubmed publisher
	Maseda D, Smith S, DiLillo D, Bryant J, Candando K, Weaver C, et al. Regulatory B10 cells differentiate into antibody-secreting cells after transient IL-10 production in vivo. J Immunol. 2012;188:1036-48 pubmed publisher
	Cox S, Stolp J, Hallahan N, Counotte J, Zhang W, Serreze D, et al. Enhanced responsiveness to T-cell help causes loss of B-lymphocyte tolerance to a ?-cell neo-self-antigen in type 1 diabetes prone NOD mice. Eur J Immunol. 2010;40:3413-25 pubmed publisher
	DeKoter R, Geadah M, Khoosal S, Xu L, Thillainadesan G, Torchia J, et al. Regulation of follicular B cell differentiation by the related E26 transformation-specific transcription factors PU.1, Spi-B, and Spi-C. J Immunol. 2010;185:7374-84 pubmed publisher
	Smith S, Haas K, Poe J, Yanaba K, Ward C, Migone T, et al. B-cell homeostasis requires complementary CD22 and BLyS/BR3 survival signals. Int Immunol. 2010;22:681-91 pubmed publisher
	Roundy K, Jacobson A, Weis J, Weis J. The in vitro derivation of phenotypically mature and diverse B cells from immature spleen and bone marrow precursors. Eur J Immunol. 2010;40:1139-49 pubmed publisher
	Minard Colin V, Xiu Y, Poe J, Horikawa M, Magro C, Hamaguchi Y, et al. Lymphoma depletion during CD20 immunotherapy in mice is mediated by macrophage FcgammaRI, FcgammaRIII, and FcgammaRIV. Blood. 2008;112:1205-13 pubmed publisher
	Treese C, Mittag A, Lange F, Tarnok A, Loesche A, Emmrich F, et al. Characterization of fibroblasts responsible for cartilage destruction in arthritis. Cytometry A. 2008;73:351-60 pubmed publisher
	Chen X, Esplin B, Garrett K, Welner R, Webb C, Kincade P. Retinoids accelerate B lineage lymphoid differentiation. J Immunol. 2008;180:138-45 pubmed
	Kozono Y, Abe R, Kozono H, Kelly R, Azuma T, Holers V. Cross-linking CD21/CD35 or CD19 increases both B7-1 and B7-2 expression on murine splenic B cells. J Immunol. 1998;160:1565-72 pubmed
	Martin B, Weis J. Murine macrophages lack expression of the Cr2-145 (CR2) and Cr2-190 (CR1) gene products. Eur J Immunol. 1993;23:3037-42 pubmed

product information

Product Type :

Antibody

Product Name :

CD21/CD35 Monoclonal Antibody (eBio8D9 (8D9)), eBioscience™

Catalog # :

14-0211-81

Quantity :

50µg

Price :

US 74.5

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Rat

Reactivity :

Mouse

Applications :

Flow Cytometry: 0.5 µg/test, Functional assay: Assay-Dependent, Western Blot: Assay-Dependent

Species :

Mouse

Clone :

eBio8D9 (8D9)

Isotype :

IgG2a, lambda

Storage :

4° C

Description :

CD21 (complement receptor 2, CR2) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to folicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands. CD35 encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.5 µg/test, Functional assay: Assay-Dependent, Western Blot: Assay-Dependent

Aliases :

C3b/C4b receptor; C3-binding protein; C3DR; CD21; CD35; CD35 antigen; complement C3d receptor; complement receptor 2; complement receptor type 1; complement receptor type 2; Cr1; Cr-1; Cr2; Cr-2; EBV receptor; expressed in B lymphocytes

more info or order :

Invitrogen product webpage