CD20 Monoclonal Antibody (AISB12), eBioscience™ | Invitrogen 14-0201-82 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD20 Monoclonal Antibody (AISB12), eBioscience™

catalog :

14-0201-82

quantity :

100µg

price :

US 113

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

AISB12

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, flow cytometry, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:100	Eberle M, Ebel P, Wegner M, MÃ¤nnich J, Tafferner N, Ferreirós N, et al. Regulation of ceramide synthase 6 in a spontaneous experimental autoimmune encephalomyelitis model is sex dependent. Biochem Pharmacol. 2014;92:326-35 pubmed publisher
flow cytometry; mouse; fig s3	Dai M, Yip Y, Hellstrom I, Hellstrom K. Curing mice with large tumors by locally delivering combinations of immunomodulatory antibodies. Clin Cancer Res. 2015;21:1127-38 pubmed publisher
flow cytometry; human	Kläsener K, Maity P, Hobeika E, Yang J, Reth M. B cell activation involves nanoscale receptor reorganizations and inside-out signaling by Syk. elife. 2014;3:e02069 pubmed publisher
blocking or activating experiments; mouse	Xie Y, Wang L, Freywald A, Qureshi M, Chen Y, Xiang J. A novel T cell-based vaccine capable of stimulating long-term functional CTL memory against B16 melanoma via CD40L signaling. Cell Mol Immunol. 2013;10:72-7 pubmed publisher
	Van Belle K, Herman J, Waer M, Sprangers B, Louat T. OSU-T315 as an Interesting Lead Molecule for Novel B Cell-Specific Therapeutics. J Immunol Res. 2018;2018:2505818 pubmed publisher
	Volkmann C, Brings N, Becker M, Hobeika E, Yang J, Reth M. Molecular requirements of the B-cell antigen receptor for sensing monovalent antigens. EMBO J. 2016;35:2371-2381 pubmed
	Manzur S, Cohen S, Haimovich J, Hollander N. Enhanced therapeutic effect of B cell-depleting anti-CD20 antibodies upon combination with in-situ dendritic cell vaccination in advanced lymphoma. Clin Exp Immunol. 2012;170:291-9 pubmed publisher
	Qian J, Zheng Y, Zheng C, Wang L, Qin H, Hong S, et al. Active vaccination with Dickkopf-1 induces protective and therapeutic antitumor immunity in murine multiple myeloma. Blood. 2012;119:161-9 pubmed publisher
	Wang C, Wang M, Liu Y, Zeng P. Administration of adenovirus encoding anti-CD20 antibody gene induces B-cell deletion and alleviates lupus in the BWF1 mouse model. Int Immunopharmacol. 2011;11:693-7 pubmed publisher
	Mishima Y, Ishihara S, Aziz M, Oka A, Kusunoki R, Otani A, et al. Decreased production of interleukin-10 and transforming growth factor-? in Toll-like receptor-activated intestinal B cells in SAMP1/Yit mice. Immunology. 2010;131:473-87 pubmed publisher
	Uchida J, Hamaguchi Y, Oliver J, Ravetch J, Poe J, Haas K, et al. The innate mononuclear phagocyte network depletes B lymphocytes through Fc receptor-dependent mechanisms during anti-CD20 antibody immunotherapy. J Exp Med. 2004;199:1659-69 pubmed
	Uchida J, Lee Y, Hasegawa M, Liang Y, Bradney A, Oliver J, et al. Mouse CD20 expression and function. Int Immunol. 2004;16:119-29 pubmed
	Tedder T, Klejman G, Disteche C, Adler D, Schlossman S, Saito H. Cloning of a complementary DNA encoding a new mouse B lymphocyte differentiation antigen, homologous to the human B1 (CD20) antigen, and localization of the gene to chromosome 19. J Immunol. 1988;141:4388-94 pubmed

product information

Product Type :

Antibody

Product Name :

CD20 Monoclonal Antibody (AISB12), eBioscience™

Catalog # :

14-0201-82

Quantity :

100µg

Price :

US 113

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Rat

Reactivity :

Mouse, Rat

Applications :

Flow Cytometry: Assay-Dependent, Western Blot: 1:1,000

Species :

Mouse, Rat

Clone :

AISB12

Isotype :

IgG2a

Storage :

4° C

Description :

CD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. CD20 is expressed on mature and most malignant B cells, in a subpopulation of T lymphocytes and follicular dendritic cells. CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation. CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment. Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-Dependent, Western Blot: 1:1,000

Aliases :

AA960661; B1; B-cell differentiation antigen Ly-44; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; BOS_15492; Bp35; Cd20; CD20 antigen; CD20 cell surface protein; CD20 receptor; CVID5; EGK_06167; LEU-16; Leukocyte surface antigen Leu-16; Ly-44; Lymphocyte antigen 44; membrane spanning 4-domains A1; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; membrane-spanning 4-domains, subfamily A, member 2; MGC3969; Ms4a1; Ms4a2; S7

more info or order :

Invitrogen product webpage