CD11b (activation epitope) Monoclonal Antibody (CBRM1/5), eBioscience™ | Invitrogen 14-0113-81 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD11b (activation epitope) Monoclonal Antibody (CBRM1/5), eBioscience™

catalog :

14-0113-81

quantity :

50 µg

price :

US 309.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CBRM1/5

reactivity :

human

application :

neutralization, immunoprecipitation, flow cytometry, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 24

Published Application/Species/Sample/Dilution	Reference
blocking or activating experiments; human; loading ...; fig 5c	Yeap W, Wong K, Shimasaki N, Teo E, Quek J, Yong H, et al. CD16 is indispensable for antibody-dependent cellular cytotoxicity by human monocytes. Sci Rep. 2016;6:34310 pubmed publisher
flow cytometry; human; 1:5; loading ...	Lu X, Chen Q, Rong Y, Yang G, Li C, Xu N, et al. LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells. Nat Commun. 2016;7:12719 pubmed publisher
flow cytometry; human; fig 6	Nakayama H, Yoshizaki F, Prinetti A, Sonnino S, Mauri L, Takamori K, et al. Lyn-coupled LacCer-enriched lipid rafts are required for CD11b/CD18-mediated neutrophil phagocytosis of nonopsonized microorganisms. J Leukoc Biol. 2008;83:728-41 pubmed
flow cytometry; human	Sendide K, Reiner N, Lee J, Bourgoin S, Talal A, Hmama Z. Cross-talk between CD14 and complement receptor 3 promotes phagocytosis of mycobacteria: regulation by phosphatidylinositol 3-kinase and cytohesin-1. J Immunol. 2005;174:4210-9 pubmed
	He L, Fu Y, Deng J, Shen Y, Wang Y, Yu F, et al. Deficiency of FAM3D (Family With Sequence Similarity 3, Member D), A Novel Chemokine, Attenuates Neutrophil Recruitment and Ameliorates Abdominal Aortic Aneurysm Development. Arterioscler Thromb Vasc Biol. 2018;38:1616-1631 pubmed publisher
	Niemann S, Bertling A, Brodde M, Fender A, Van de Vyver H, Hussain M, et al. Panton-Valentine Leukocidin associated with S. aureus osteomyelitis activates platelets via neutrophil secretion products. Sci Rep. 2018;8:2185 pubmed publisher
	Zhu N, Wang H, Wang B, Wei J, Shan W, Feng J, et al. A Member of the Nuclear Receptor Superfamily, Designated as NR2F2, Supports the Self-Renewal Capacity and Pluripotency of Human Bone Marrow-Derived Mesenchymal Stem Cells. Stem Cells Int. 2016;2016:5687589 pubmed publisher
	Zeng C, Chen Z, Zhang X, Han B, Lin K, Li X, et al. MIR125B1 represses the degradation of the PML-RARA oncoprotein by an autophagy-lysosomal pathway in acute promyelocytic leukemia. Autophagy. 2014;10:1726-37 pubmed publisher
	Brancaleone V, Mitidieri E, Flower R, Cirino G, Perretti M. Annexin A1 mediates hydrogen sulfide properties in the control of inflammation. J Pharmacol Exp Ther. 2014;351:96-104 pubmed publisher
	Zhou Y, Wu J, Kucik D, White N, Redden D, Szalai A, et al. Multiple lupus-associated ITGAM variants alter Mac-1 functions on neutrophils. Arthritis Rheum. 2013;65:2907-16 pubmed publisher
	Futosi K, Nemeth T, Pick R, Vantus T, Walzog B, Mocsai A. Dasatinib inhibits proinflammatory functions of mature human neutrophils. Blood. 2012;119:4981-91 pubmed publisher
	Yan T, Li Q, Zhou H, Zhao Y, Yu S, Xu G, et al. Gu-4 suppresses affinity and avidity modulation of CD11b and improves the outcome of mice with endotoxemia and sepsis. PLoS ONE. 2012;7:e30110 pubmed publisher
	Acharya S, Kaplan R, Macdonald D, Fabiyi O, DiMichele D, Lyden D. Neoangiogenesis contributes to the development of hemophilic synovitis. Blood. 2011;117:2484-93 pubmed publisher
	Hajishengallis G, Wang M, Liang S. Induction of distinct TLR2-mediated proinflammatory and proadhesive signaling pathways in response to Porphyromonas gingivalis fimbriae. J Immunol. 2009;182:6690-6 pubmed publisher
	Patel P, Harrison R. Membrane ruffles capture C3bi-opsonized particles in activated macrophages. Mol Biol Cell. 2008;19:4628-39 pubmed publisher
	Meliton A, Munoz N, Leff A. Blockade of avidity and focal clustering of beta 2-integrin by cysteinyl leukotriene antagonism attenuates eosinophil adhesion. J Allergy Clin Immunol. 2007;120:1316-23 pubmed
	Lishko V, Burke T, Ugarova T. Antiadhesive effect of fibrinogen: a safeguard for thrombus stability. Blood. 2007;109:1541-9 pubmed
	de Boer H, Verseyden C, Ulfman L, Zwaginga J, Bot I, Biessen E, et al. Fibrin and activated platelets cooperatively guide stem cells to a vascular injury and promote differentiation towards an endothelial cell phenotype. Arterioscler Thromb Vasc Biol. 2006;26:1653-9 pubmed
	Lomakina E, Waugh R. Micromechanical tests of adhesion dynamics between neutrophils and immobilized ICAM-1. Biophys J. 2004;86:1223-33 pubmed
	Cox D, Dale B, Kashiwada M, Helgason C, Greenberg S. A regulatory role for Src homology 2 domain-containing inositol 5'-phosphatase (SHIP) in phagocytosis mediated by Fc gamma receptors and complement receptor 3 (alpha(M)beta(2); CD11b/CD18). J Exp Med. 2001;193:61-71 pubmed
	Whitlock B, Gardai S, Fadok V, Bratton D, Henson P. Differential roles for alpha(M)beta(2) integrin clustering or activation in the control of apoptosis via regulation of akt and ERK survival mechanisms. J Cell Biol. 2000;151:1305-20 pubmed
	Oxvig C, Lu C, Springer T. Conformational changes in tertiary structure near the ligand binding site of an integrin I domain. Proc Natl Acad Sci U S A. 1999;96:2215-20 pubmed
	Weber C, Kitayama J, Springer T. Differential regulation of beta 1 and beta 2 integrin avidity by chemoattractants in eosinophils. Proc Natl Acad Sci U S A. 1996;93:10939-44 pubmed
	Diamond M, Springer T. A subpopulation of Mac-1 (CD11b/CD18) molecules mediates neutrophil adhesion to ICAM-1 and fibrinogen. J Cell Biol. 1993;120:545-56 pubmed

product information

Product Type :

Antibody

Product Name :

CD11b (activation epitope) Monoclonal Antibody (CBRM1/5), eBioscience™

Catalog # :

14-0113-81

Quantity :

50 µg

Price :

US 309.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: 1 µg/test, Functional assay: Assay-Dependent, Immunoprecipitation: Assay-Dependent, Neutralization: Assay-Dependent

Species :

Human

Clone :

CBRM1/5

Isotype :

IgG1, kappa

Storage :

4° C

Description :

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1 µg/test, Functional assay: Assay-Dependent, Immunoprecipitation: Assay-Dependent, Neutralization: Assay-Dependent

Aliases :

antigen CD11b (p170); antigen CD11b (p170); macrophage antigen alpha polypeptide; CD11 antigen-like family member B; CD11b; CD11B (p170); CD11b/CD18; cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; complement component receptor 3 alpha-a; complement receptor type 3; CR3; CR-3 alpha chain; CR3A; F730045J24Rik; integrin alpha M; integrin alpha-M; integrin subunit alpha M; integrin, alpha M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; leukocyte integrin alpha-M chain; LOC100351865; Ly-40; MAC1; Mac-1; Mac-1 alpha; Mac-1 alpha (Mac1A); MAC-1 alpha subunit; MAC1A; Mac-1a; macrophage antigen alpha; macrophage antigen alpha polypeptide; MGC117044; MO1A; Neutrophil adherence receptor; neutrophil adherence receptor alpha-M subunit; SLEB6; unnamed protein product

more info or order :

Invitrogen product webpage