product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), eBioscience™
catalog :
14-0083-85
quantity :
500 µg
price :
US 309.00
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
eBioH35-17.2 (H35-17.2)
reactivity :
mouse
application :
immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments
more info or order :
Invitrogen product webpage
citations: 42
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:400; fig s1g
Li A, Herbst R, Canner D, Schenkel J, Smith O, Kim J, et al. IL-33 Signaling Alters Regulatory T Cell Diversity in Support of Tumor Development. Cell Rep. 2019;29:2998-3008.e8 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4c, 5c
Cornelissen L, Blanas A, van der Horst J, Kruijssen L, Zaal A, O Toole T, et al. Disruption of sialic acid metabolism drives tumor growth by augmenting CD8+ T cell apoptosis. Int J Cancer. 2019;144:2290-2302 pubmed publisher
  • flow cytometry; mouse; loading ...; fig S4
Huang A, Peng D, Guo H, Ben Y, Zuo X, Wu F, et al. A human programmed death-ligand 1-expressing mouse tumor model for evaluating the therapeutic efficacy of anti-human PD-L1 antibodies. Sci Rep. 2017;7:42687 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Ma C, Mishra S, Demel E, Liu Y, Zhang N. TGF-? Controls the Formation of Kidney-Resident T Cells via Promoting Effector T Cell Extravasation. J Immunol. 2017;198:749-756 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Hwang S, Cobb D, Bhadra R, Youngblood B, Khan I. Blimp-1-mediated CD4 T cell exhaustion causes CD8 T cell dysfunction during chronic toxoplasmosis. J Exp Med. 2016;213:1799-818 pubmed publisher
  • immunohistochemistry; mouse; 1:75; loading ...; fig 3e
Zhang Y, Velez Delgado A, Mathew E, Li D, Mendez F, Flannagan K, et al. Myeloid cells are required for PD-1/PD-L1 checkpoint activation and the establishment of an immunosuppressive environment in pancreatic cancer. Gut. 2017;66:124-136 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1a
Sujino T, London M, Hoytema van Konijnenburg D, Rendon T, Buch T, Silva H, et al. Tissue adaptation of regulatory and intraepithelial CD4? T cells controls gut inflammation. Science. 2016;352:1581-6 pubmed publisher
  • blocking or activating experiments; mouse; loading ...; fig 3
Azpilikueta A, Agorreta J, Labiano S, Pérez Gracia J, Sánchez Paulete A, Aznar M, et al. Successful Immunotherapy against a Transplantable Mouse Squamous Lung Carcinoma with Anti-PD-1 and Anti-CD137 Monoclonal Antibodies. J Thorac Oncol. 2016;11:524-36 pubmed publisher
  • flow cytometry; mouse
Masuda T, Wang X, Maeda M, Canver M, Sher F, Funnell A, et al. Transcription factors LRF and BCL11A independently repress expression of fetal hemoglobin. Science. 2016;351:285-9 pubmed publisher
  • flow cytometry; mouse; fig 2
Moretto M, Khan I. IL-21 Is Important for Induction of KLRG1+ Effector CD8 T Cells during Acute Intracellular Infection. J Immunol. 2016;196:375-84 pubmed publisher
  • flow cytometry; mouse; fig s2
Wensveen F, Jelenčić V, Valentić S, Šestan M, Wensveen T, Theurich S, et al. NK cells link obesity-induced adipose stress to inflammation and insulin resistance. Nat Immunol. 2015;16:376-85 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 1
LUCAS B, White A, Ulvmar M, Nibbs R, Sitnik K, Agace W, et al. CCRL1/ACKR4 is expressed in key thymic microenvironments but is dispensable for T lymphopoiesis at steady state in adult mice. Eur J Immunol. 2015;45:574-83 pubmed publisher
  • flow cytometry; mouse; fig 2
Harimoto H, Shimizu M, Nakagawa Y, Nakatsuka K, Wakabayashi A, Sakamoto C, et al. Inactivation of tumor-specific CD8? CTLs by tumor-infiltrating tolerogenic dendritic cells. Immunol Cell Biol. 2013;91:545-55 pubmed publisher
  • flow cytometry; mouse; fig 5
Yan S, Wang L, Liu N, Wang Y, Chu Y. Critical role of interleukin-17/interleukin-17 receptor axis in mediating Con A-induced hepatitis. Immunol Cell Biol. 2012;90:421-8 pubmed publisher
  • flow cytometry; mouse
Ellestad K, Tsutsui S, Noorbakhsh F, Warren K, Yong V, Pittman Q, et al. Early life exposure to lipopolysaccharide suppresses experimental autoimmune encephalomyelitis by promoting tolerogenic dendritic cells and regulatory T cells. J Immunol. 2009;183:298-309 pubmed publisher
Wang W, Shahzad K, Li M, Zhang A, Zhang L, Xu T, et al. An Antigen-Presenting and Apoptosis-Inducing Polymer Microparticle Prolongs Alloskin Graft Survival by Selectively and Markedly Depleting Alloreactive CD8+ T Cells. Front Immunol. 2017;8:657 pubmed publisher
Overacre Delgoffe A, Chikina M, Dadey R, Yano H, Brunazzi E, Shayan G, et al. Interferon-? Drives Treg Fragility to Promote Anti-tumor Immunity. Cell. 2017;169:1130-1141.e11 pubmed publisher
Macpherson K, Sompol P, Kannarkat G, Chang J, Sniffen L, Wildner M, et al. Peripheral administration of the soluble TNF inhibitor XPro1595 modifies brain immune cell profiles, decreases beta-amyloid plaque load, and rescues impaired long-term potentiation in 5xFAD mice. Neurobiol Dis. 2017;102:81-95 pubmed publisher
Zamarin D, Holmgaard R, Ricca J, Plitt T, Palese P, Sharma P, et al. Intratumoral modulation of the inducible co-stimulator ICOS by recombinant oncolytic virus promotes systemic anti-tumour immunity. Nat Commun. 2017;8:14340 pubmed publisher
Vicetti Miguel R, Quispe Calla N, Pavelko S, Cherpes T. Intravaginal Chlamydia trachomatis Challenge Infection Elicits TH1 and TH17 Immune Responses in Mice That Promote Pathogen Clearance and Genital Tract Damage. PLoS ONE. 2016;11:e0162445 pubmed publisher
Cao W, Guo J, Wen X, Miao L, Lin F, Xu G, et al. CXXC finger protein 1 is critical for T-cell intrathymic development through regulating H3K4 trimethylation. Nat Commun. 2016;7:11687 pubmed publisher
Polansky J, Bahri R, Divivier M, Duitman E, Vock C, Goyeneche Patino D, et al. High dose CD11c-driven IL15 is sufficient to drive NK cell maturation and anti-tumor activity in a trans-presentation independent manner. Sci Rep. 2016;6:19699 pubmed publisher
Sell S, Guest I, McKinstry K, Strutt T, Kohlmeier J, Brincks E, et al. Intraepithelial T-cell cytotoxicity, induced bronchus-associated lymphoid tissue, and proliferation of pneumocytes in experimental mouse models of influenza. Viral Immunol. 2014;27:484-96 pubmed publisher
Chen L, Gibbons D, Goswami S, Cortez M, Ahn Y, Byers L, et al. Metastasis is regulated via microRNA-200/ZEB1 axis control of tumour cell PD-L1 expression and intratumoral immunosuppression. Nat Commun. 2014;5:5241 pubmed publisher
Bhadra R, Moretto M, Castillo J, Petrovas C, Ferrando Martinez S, Shokal U, et al. Intrinsic TGF-? signaling promotes age-dependent CD8+ T cell polyfunctionality attrition. J Clin Invest. 2014;124:2441-55 pubmed publisher
Eckhardt J, Kreiser S, Döbbeler M, Nicolette C, DeBenedette M, Tcherepanova I, et al. Soluble CD83 ameliorates experimental colitis in mice. Mucosal Immunol. 2014;7:1006-18 pubmed publisher
Pioli P, Dahlem T, Weis J, Weis J. Deletion of Snai2 and Snai3 results in impaired physical development compounded by lymphocyte deficiency. PLoS ONE. 2013;8:e69216 pubmed publisher
Bhadra R, Cobb D, Khan I. Donor CD8+ T cells prevent Toxoplasma gondii de-encystation but fail to rescue the exhausted endogenous CD8+ T cell population. Infect Immun. 2013;81:3414-25 pubmed publisher
Koni P, Bolduc A, Takezaki M, Ametani Y, Huang L, Lee J, et al. Constitutively CD40-activated B cells regulate CD8 T cell inflammatory response by IL-10 induction. J Immunol. 2013;190:3189-96 pubmed publisher
van der Touw W, Burrell B, Lal G, Bromberg J. NK cells are required for costimulatory blockade induced tolerance to vascularized allografts. Transplantation. 2012;94:575-84 pubmed
Diaconu I, Cerullo V, Hirvinen M, Escutenaire S, Ugolini M, Pesonen S, et al. Immune response is an important aspect of the antitumor effect produced by a CD40L-encoding oncolytic adenovirus. Cancer Res. 2012;72:2327-38 pubmed publisher
Morello S, Sorrentino R, Montinaro A, Luciano A, Maiolino P, Ngkelo A, et al. NK1.1 cells and CD8 T cells mediate the antitumor activity of Cl-IB-MECA in a mouse melanoma model. Neoplasia. 2011;13:365-73 pubmed
de Sousa A, Reis R, Bento Torres J, Trévia N, Lins N, Passos A, et al. Influence of enriched environment on viral encephalitis outcomes: behavioral and neuropathological changes in albino Swiss mice. PLoS ONE. 2011;6:e15597 pubmed publisher
Liu H, Chi A, Arnett K, Chiang M, Xu L, Shestova O, et al. Notch dimerization is required for leukemogenesis and T-cell development. Genes Dev. 2010;24:2395-407 pubmed publisher
Findlay E, Greig R, Stumhofer J, Hafalla J, de Souza J, Saris C, et al. Essential role for IL-27 receptor signaling in prevention of Th1-mediated immunopathology during malaria infection. J Immunol. 2010;185:2482-92 pubmed publisher
Shang L, Thirunarayanan N, Viejo Borbolla A, Martin A, Bogunovic M, Marchesi F, et al. Expression of the chemokine binding protein M3 promotes marked changes in the accumulation of specific leukocytes subsets within the intestine. Gastroenterology. 2009;137:1006-18, 1018.e1-3 pubmed publisher
Archambaud C, Sansoni A, Mingueneau M, Devilard E, Delsol G, Malissen B, et al. STAT6 deletion converts the Th2 inflammatory pathology afflicting Lat(Y136F) mice into a lymphoproliferative disorder involving Th1 and CD8 effector T cells. J Immunol. 2009;182:2680-9 pubmed publisher
Thoma Uszynski S, Emoto M, Kaufmann S. CD8alphaalpha T cells in lesions of Listeria monocytogenes-infected beta2m-deficient mice. Microb Pathog. 1997;23:101-6 pubmed
Tarleton R, Sun J, Zhang L, Postan M. Depletion of T-cell subpopulations results in exacerbation of myocarditis and parasitism in experimental Chagas' disease. Infect Immun. 1994;62:1820-9 pubmed
Golstein P, Goridis C, Schmitt Verhulst A, Hayot B, Pierres A, van Agthoven A, et al. Lymphoid cell surface interaction structures detected using cytolysis-inhibiting monoclonal antibodies. Immunol Rev. 1982;68:5-42 pubmed
Ledbetter J, Seaman W, Tsu T, Herzenberg L. Lyt-2 and lyt-3 antigens are on two different polypeptide subunits linked by disulfide bonds. Relationship of subunits to T cell cytolytic activity. J Exp Med. 1981;153:1503-16 pubmed
Lefrancois L. Phenotypic complexity of intraepithelial lymphocytes of the small intestine. J Immunol. 1991;147:1746-51 pubmed
product information
Product Type :
Antibody
Product Name :
CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), eBioscience™
Catalog # :
14-0083-85
Quantity :
500 µg
Price :
US 309.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.5 µg/test, Immunohistochemistry (Frozen): Assay-Dependent, Immunoprecipitation: Assay-Dependent
Species :
Mouse
Clone :
eBioH35-17.2 (H35-17.2)
Isotype :
IgG2b, kappa
Storage :
4° C
Description :
The CD8B antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.5 µg/test, Immunohistochemistry (Frozen): Assay-Dependent, Immunoprecipitation: Assay-Dependent
Aliases :
CD8; CD8 antigen 37 kDa chain; CD8 antigen beta polypeptide; CD8 antigen beta polypeptide precursor; CD8 antigen beta-chain; CD8 antigen, beta chain; CD8 antigen, beta chain 1; CD8 antigen, beta polypeptide 1 (p37); CD8 antigen, beta-chain; CD8 beta; CD8 beta chain; CD8a; CD8alpha; CD8B; CD8b antigen; CD8b molecule; CD8b molecule pseudogene; CD8B1; CD8beta; CD8BP; LEU2; Leu-2; LY3; Ly-3; Ly-C; Lymphocyte antigen 3; Lyt3; Lyt-3; MAL; OX-8 membrane antigen; p32; P37; T lymphocyte surface glycoprotein beta chain; T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-3
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA