CD2 Monoclonal Antibody (RPA-2.10), eBioscience | Invitrogen 14-0029-82 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD2 Monoclonal Antibody (RPA-2.10), eBioscience

catalog :

14-0029-82

quantity :

100 µg

price :

US 133

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

RPA-2.10

reactivity :

crab eating macaque, baboon , human, mouse, chimpanzee, pigs , rhesus macaque

application :

western blot, immunohistochemistry, neutralization, flow cytometry, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 22

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; fig 7	Venkatasubramanian S, Tripathi D, Tucker T, Paidipally P, Cheekatla S, Welch E, et al. Tissue factor expression by myeloid cells contributes to protective immune response against Mycobacterium tuberculosis infection. Eur J Immunol. 2016;46:464-79 pubmed publisher
flow cytometry; mouse; loading ...	Okoye Okafor U, Bartholdy B, Cartier J, Gao E, Pietrak B, Rendina A, et al. New IDH1 mutant inhibitors for treatment of acute myeloid leukemia. Nat Chem Biol. 2015;11:878-86 pubmed publisher
flow cytometry; human	Grieco A, Billett H, Green N, Driscoll M, Bouhassira E. Variation in Gamma-Globin Expression before and after Induction with Hydroxyurea Associated with BCL11A, KLF1 and TAL1. PLoS ONE. 2015;10:e0129431 pubmed publisher
flow cytometry; human	Wilson E, Singh A, Hullsiek K, Gibson D, Henry W, Lichtenstein K, et al. Monocyte-activation phenotypes are associated with biomarkers of inflammation and coagulation in chronic HIV infection. J Infect Dis. 2014;210:1396-406 pubmed publisher
flow cytometry; human	Sereti I, Estes J, Thompson W, Morcock D, Fischl M, Croughs T, et al. Decreases in colonic and systemic inflammation in chronic HIV infection after IL-7 administration. PLoS Pathog. 2014;10:e1003890 pubmed publisher
flow cytometry; mouse	Timblin G, Schlissel M. Ebf1 and c-Myb repress rag transcription downstream of Stat5 during early B cell development. J Immunol. 2013;191:4676-87 pubmed publisher
immunohistochemistry; human	Liang H, Reinhardt R, Bando J, Sullivan B, Ho I, Locksley R. Divergent expression patterns of IL-4 and IL-13 define unique functions in allergic immunity. Nat Immunol. 2011;13:58-66 pubmed publisher
flow cytometry; human	Park C, Majeti R, Weissman I. In vivo evaluation of human hematopoiesis through xenotransplantation of purified hematopoietic stem cells from umbilical cord blood. Nat Protoc. 2008;3:1932-40 pubmed publisher
	Moriyama S, Anraku Y, Taminishi S, Adachi Y, Kuroda D, Kita S, et al. Structural delineation and computational design of SARS-CoV-2-neutralizing antibodies against Omicron subvariants. Nat Commun. 2023;14:4198 pubmed publisher
	Lim S, Kim J, Jeon S, Shin M, Kwon J, Kim T, et al. Defective Localization With Impaired Tumor Cytotoxicity Contributes to the Immune Escape of NK Cells in Pancreatic Cancer Patients. Front Immunol. 2019;10:496 pubmed publisher
	Cho J, Xu Z, Parthasarathy U, Drashansky T, Helm E, Zuniga A, et al. Hectd3 promotes pathogenic Th17 lineage through Stat3 activation and Malt1 signaling in neuroinflammation. Nat Commun. 2019;10:701 pubmed publisher
	Denton A, Innocentin S, Carr E, Bradford B, Lafouresse F, Mabbott N, et al. Type I interferon induces CXCL13 to support ectopic germinal center formation. J Exp Med. 2019;216:621-637 pubmed publisher
	Felce J, MacRae A, Davis S. Constraints on GPCR Heterodimerization Revealed by the Type-4 Induced-Association BRET Assay. Biophys J. 2019;116:31-41 pubmed publisher
	Seeholzer T, Kurz S, Schlauderer F, Woods S, Gehring T, Widmann S, et al. BCL10-CARD11 Fusion Mimics an Active CARD11 Seed That Triggers Constitutive BCL10 Oligomerization and Lymphocyte Activation. Front Immunol. 2018;9:2695 pubmed publisher
	Baumgartner C, Toifl S, Farlik M, Halbritter F, Scheicher R, Fischer I, et al. An ERK-Dependent Feedback Mechanism Prevents Hematopoietic Stem Cell Exhaustion. Cell Stem Cell. 2018;22:879-892.e6 pubmed publisher
	Kostrzewski T, Borg A, Meng Y, Filipovic I, Male V, Wack A, et al. Multiple Levels of Control Determine How E4bp4/Nfil3 Regulates NK Cell Development. J Immunol. 2018;200:1370-1381 pubmed publisher
	Wang Y, Ma C, Ling Y, Bousfiha A, Camcioglu Y, Jacquot S, et al. Dual T cell- and B cell-intrinsic deficiency in humans with biallelic RLTPR mutations. J Exp Med. 2016;213:2413-2435 pubmed
	Male V, Nisoli I, Kostrzewski T, Allan D, Carlyle J, Lord G, et al. The transcription factor E4bp4/Nfil3 controls commitment to the NK lineage and directly regulates Eomes and Id2 expression. J Exp Med. 2014;211:635-42 pubmed publisher
	Lim S, Kim T, Lee J, Sonn C, Kim K, Kim J, et al. Ex vivo expansion of highly cytotoxic human NK cells by cocultivation with irradiated tumor cells for adoptive immunotherapy. Cancer Res. 2013;73:2598-607 pubmed publisher
	De Gobbi M, Garrick D, Lynch M, Vernimmen D, Hughes J, Goardon N, et al. Generation of bivalent chromatin domains during cell fate decisions. Epigenetics Chromatin. 2011;4:9 pubmed publisher
	Kap Y, van Meurs M, van Driel N, Koopman G, Melief M, Brok H, et al. A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates. J Histochem Cytochem. 2009;57:1159-67 pubmed publisher
	Aversa G, Bishop G, Suranyi M, Hall B. RPA-2.10: an anti-CD2 monoclonal antibody that inhibits alloimmune responses and monitors T cell activation. Transplant Proc. 1987;19:277-8 pubmed

product information

Product Type :

Antibody

Product Name :

CD2 Monoclonal Antibody (RPA-2.10), eBioscience

Catalog # :

14-0029-82

Quantity :

100 µg

Price :

US 133

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Baboon, Chimpanzee, Cynomolgus Monkey, Human, Non-human primate, Porcine, Rhesus Monkey

Applications :

Flow Cytometry: 0.5 µg/test, Functional Assay: Assay-Dependent, Immunohistochemistry (Frozen): Assay-Dependent, Neutralization: Assay-Dependent, Western Blot: Assay-Dependent

Species :

Baboon, Chimpanzee, Cynomolgus Monkey, Human, Non-human primate, Porcine, Rhesus Monkey

Clone :

RPA-2.10

Isotype :

IgG1, kappa

Storage :

4° C

Description :

CD2 (LFA-2) is a monomeric surface antigen (MW range 45-58 kDa) of the human T-lymphocyte lineage that is expressed on all peripheral blood T cells. CD2 is one of the earliest T-cell markers, being present on more than 95% of thymocytes and it is also found on some natural killer cells, but not on B lymphocytes. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it. The interaction between CD2 and CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection. Diseases associated with CD2 dysfunction include penis squamous cell carcinoma and immune deficiency due to the absence of the thymus.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.5 µg/test, Functional Assay: Assay-Dependent, Immunohistochemistry (Frozen): Assay-Dependent, Neutralization: Assay-Dependent, Western Blot: Assay-Dependent

Aliases :

Cd2; CD2 antigen; CD2 antigen (p50), sheep red blood cell receptor; CD2 molecule; CD2R; cluster of differentiation 2; Erythrocyte receptor; FLJ46032; LFA2; LFA-2; LFA-3 receptor; Ly37; Ly-37; lymphocyte antigen 37; OX34; OX-34; OX-34 antigen; OX-45 surface antigen homolog to human T lymphocyte CD2 antigen; Rosette receptor; SRBC; surface immunoglobulin superfamily molecule; T11; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5

more info or order :

Invitrogen product webpage