alpha Catenin Monoclonal Antibody (alpha-CAT-7A4) | Invitrogen 13-9700 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

alpha Catenin Monoclonal Antibody (alpha-CAT-7A4)

catalog :

13-9700

quantity :

100 µg

price :

US 449.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

alpha-CAT-7A4

reactivity :

human, mouse, rat, chicken

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 32

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:250; loading ...; fig 4b	Wijshake T, Zou Z, Chen B, Zhong L, Xiao G, Xie Y, et al. Tumor-suppressor function of Beclin 1 in breast cancer cells requires E-cadherin. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
immunohistochemistry; rat; 1:100	Li N, Mruk D, Wong C, Lee W, Han D, Cheng C. Actin-bundling protein plastin 3 is a regulator of ectoplasmic specialization dynamics during spermatogenesis in the rat testis. FASEB J. 2015;29:3788-805 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100	Figueira A, Gomes C, Vilhena H, Miranda S, Carvalheira J, de Matos A, et al. Characterization of Î±-, Î²- and p120-Catenin Expression in Feline Mammary Tissues and their Relation with E- and P-Cadherin. Anticancer Res. 2015;35:3361-9 pubmed
immunohistochemistry - paraffin section; human; 1:100	da Silva S, Morand G, Alobaid F, Hier M, Mlynarek A, Alaoui Jamali M, et al. Epithelial-mesenchymal transition (EMT) markers have prognostic impact in multiple primary oral squamous cell carcinoma. Clin Exp Metastasis. 2015;32:55-63 pubmed publisher
immunohistochemistry; rat; 1:100	Qian X, Mruk D, Cheng C. Rai14 (retinoic acid induced protein 14) is involved in regulating f-actin dynamics at the ectoplasmic specialization in the rat testis*. PLoS ONE. 2013;8:e60656 pubmed publisher
immunocytochemistry; mouse; 1:100; loading ...; fig s5 immunohistochemistry; mouse; 1:100; loading ...; fig s3h	Gladden A, Hebert A, Schneeberger E, McClatchey A. The NF2 tumor suppressor, Merlin, regulates epidermal development through the establishment of a junctional polarity complex. Dev Cell. 2010;19:727-39 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100	Patriarca C, Colombo P, Pio Taronna A, Wesseling J, Franchi G, Guddo F, et al. Cell discohesion and multifocality of carcinoma in situ of the bladder: new insight from the adhesion molecule profile (e-cadherin, Ep-CAM, and MUC1). Int J Surg Pathol. 2009;17:99-106 pubmed publisher
immunohistochemistry - frozen section; rat	Uehara K, Uehara A. Localization of claudin-5 and ZO-1 in rat spleen sinus endothelial cells. Histochem Cell Biol. 2008;129:95-103 pubmed
immunohistochemistry - paraffin section; human; 1:100; fig 1	Canavese G, Bernardi A, Candelaresi G, Lovadina P, Amerio S, Rossetti V, et al. Expression of the E-cadherin-catenins complex in sentinel node is related to tumor morphology but not to spread to nonsentinel nodes. Pathol Res Pract. 2007;203:517-23 pubmed
immunohistochemistry - frozen section; mouse; 1:100	Akins M, Greer C. Axon behavior in the olfactory nerve reflects the involvement of catenin-cadherin mediated adhesion. J Comp Neurol. 2006;499:979-89 pubmed
immunocytochemistry; mouse; fig 1 western blot; mouse; fig 1	Kim Y, Sauer C, Testa K, Wahl J, Svoboda R, Johnson K, et al. Modulating the strength of cadherin adhesion: evidence for a novel adhesion complex. J Cell Sci. 2005;118:3883-94 pubmed
immunohistochemistry - frozen section; human	Kaplan S, Gard J, Protonotarios N, Tsatsopoulou A, Spiliopoulou C, Anastasakis A, et al. Remodeling of myocyte gap junctions in arrhythmogenic right ventricular cardiomyopathy due to a deletion in plakoglobin (Naxos disease). Heart Rhythm. 2004;1:3-11 pubmed
immunohistochemistry - paraffin section; human; 1:50; tbl 1	Marques F, Fonsechi Carvasan G, de Angelo Andrade L, Bottcher Luiz F. Immunohistochemical patterns for alpha- and beta-catenin, E- and N-cadherin expression in ovarian epithelial tumors. Gynecol Oncol. 2004;94:16-24 pubmed
immunohistochemistry - paraffin section; human; 1:100; tbl 4	Song S, Kim S, Kim D, Son H, Rhee J, Kim Y. Abnormal expression of E-cadherin in early gastric carcinoma: its relationship with macroscopic growth patterns and catenin alpha and beta. J Clin Gastroenterol. 2004;38:252-9 pubmed
western blot; mouse; fig 8	Carothers A, Melstrom K, Mueller J, Weyant M, Bertagnolli M. Progressive changes in adherens junction structure during intestinal adenoma formation in Apc mutant mice. J Biol Chem. 2001;276:39094-102 pubmed
immunocytochemistry; human	Allport J, Muller W, Luscinskas F. Monocytes induce reversible focal changes in vascular endothelial cadherin complex during transendothelial migration under flow. J Cell Biol. 2000;148:203-16 pubmed
immunohistochemistry - frozen section; human	Spirin K, Ljubimov A, Castellon R, Wiedoeft O, Marano M, Sheppard D, et al. Analysis of gene expression in human bullous keratopathy corneas containing limiting amounts of RNA. Invest Ophthalmol Vis Sci. 1999;40:3108-15 pubmed
western blot; human	de Boer C, van Dorst E, van Krieken H, Jansen van Rhijn C, Warnaar S, Fleuren G, et al. Changing roles of cadherins and catenins during progression of squamous intraepithelial lesions in the uterine cervix. Am J Pathol. 1999;155:505-15 pubmed
	Ouyang X, Dong C, Ubogu E. In situ molecular characterization of endoneurial microvessels that form the blood-nerve barrier in normal human adult peripheral nerves. J Peripher Nerv Syst. 2019;24:195-206 pubmed publisher
	Anchan A, Kalogirou Baldwin P, Johnson R, Kho D, Joseph W, Hucklesby J, et al. Real-Time Measurement of Melanoma Cell-Mediated Human Brain Endothelial Barrier Disruption Using Electric Cell-Substrate Impedance Sensing Technology. Biosensors (Basel). 2019;9: pubmed publisher
	Domke L, Franke W. The cell-cell junctions of mammalian testes: II. The lamellar smooth muscle monolayer cells of the peritubular wall are laterally connected by vertical adherens junctions-a novel architectonic cell-cell junction system. Cell Tissue Res. 2019;375:451-482 pubmed publisher
	Chen H, Lu R, Zhang Y, Sun J. Vitamin D Receptor Deletion Leads to the Destruction of Tight and Adherens Junctions in Lungs. Tissue Barriers. 2018;6:1-13 pubmed publisher
	Robilliard L, Kho D, Johnson R, Anchan A, O Carroll S, Graham E. The Importance of Multifrequency Impedance Sensing of Endothelial Barrier Formation Using ECIS Technology for the Generation of a Strong and Durable Paracellular Barrier. Biosensors (Basel). 2018;8: pubmed publisher
	Vite A, Zhang C, Yi R, Emms S, Radice G. α-Catenin-dependent cytoskeletal tension controls Yap activity in the heart. Development. 2018;145: pubmed publisher
	Palladino S, Helton E, Jain P, Dong C, Crowley M, Crossman D, et al. The Human Blood-Nerve Barrier Transcriptome. Sci Rep. 2017;7:17477 pubmed publisher
	Advedissian T, Proux Gillardeaux V, Nkosi R, Peyret G, Nguyen T, Poirier F, et al. E-cadherin dynamics is regulated by galectin-7 at epithelial cell surface. Sci Rep. 2017;7:17086 pubmed publisher
	Jenkins E, Caputo M, Angelini G, Ghorbel M. Chronic hypoxia down-regulates tight junction protein ZO-2 expression in children with cyanotic congenital heart defect. ESC Heart Fail. 2016;3:131-137 pubmed
	Qian X, Mruk D, Wong E, Lie P, Cheng C. Palladin is a regulator of actin filament bundles at the ectoplasmic specialization in adult rat testes. Endocrinology. 2013;154:1907-20 pubmed publisher
	Troyanovsky R, Klingelhofer J, Troyanovsky S. ?-Catenin contributes to the strength of E-cadherin-p120 interactions. Mol Biol Cell. 2011;22:4247-55 pubmed publisher
	Gould Rothberg B, Berger A, Molinaro A, Subtil A, Krauthammer M, Camp R, et al. Melanoma prognostic model using tissue microarrays and genetic algorithms. J Clin Oncol. 2009;27:5772-80 pubmed publisher
	Jacquemier J, Ginestier C, Rougemont J, Bardou V, Charafe Jauffret E, Geneix J, et al. Protein expression profiling identifies subclasses of breast cancer and predicts prognosis. Cancer Res. 2005;65:767-79 pubmed
	Scheffers M, Le H, van der Bent P, Leonhard W, Prins F, Spruit L, et al. Distinct subcellular expression of endogenous polycystin-2 in the plasma membrane and Golgi apparatus of MDCK cells. Hum Mol Genet. 2002;11:59-67 pubmed

product information

Product Type :

Antibody

Product Name :

alpha Catenin Monoclonal Antibody (alpha-CAT-7A4)

Catalog # :

13-9700

Quantity :

100 µg

Price :

US 449.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Chicken, Human, Mouse, Rat, Xenopus laevis

Applications :

ELISA: Assay-dependent, Flow Cytometry: 3-5 µg/1x10^6 cells, Immunocytochemistry: 1:250, Immunohistochemistry: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:250

Species :

Chicken, Human, Mouse, Rat, Xenopus laevis

Clone :

alpha-CAT-7A4

Isotype :

IgG1, kappa

Storage :

-20°C

Description :

Alpha catenin is a member of the b-catenin family of cytosolic proteins. Alpha catenin interacts with N-cadherin and b-catenin on the cytosolic side of adherins junctions to mediate the interaction with the actin cytoskeleton. Alpha catenin is a homodimer in solution, but dissociates to form a 1:1 heterodimer with b-catenin in order to interact with cadherins. It also regulates morphological plasticity of synapses and cerebellar and hippocampal lamination during development. Alpha catenin also functions in the control of the startle modulation.

Immunogen :

Synthetic peptide derived from the C-terminus of mouse alpha-catenin.

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Flow Cytometry: 3-5 µg/1x10^6 cells, Immunocytochemistry: 1:250, Immunohistochemistry: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:250

Aliases :

102 kDa cadherin-associated protein; 2010010M04Rik; AA517462; AI481747; AI988031; alpha E catenin; alpha E-catenin; Alpha N-catenin; alpha(E)-catenin; alpha(N)-catenin; alpha-catenin; alpha-catenin-related protein; alpha-E-catenin; alphaE-catenin; alpha-N-catenin; alphaN-catenin; cadherin associated protein; Cadherin-associated protein; cadherin-associated protein, related; cancer/testis antigen 114; CAP102; CAPR; CAP-R; catenin; catenin (cadherin associated protein), alpha 1; catenin (cadherin associated protein), alpha 2; catenin (cadherin-associated protein), alpha 1, 102kDa; catenin (cadherin-associated protein), alpha 2; catenin alpha 1; catenin alpha 2; Catenin alpha1; catenin alpha-1; Catenin alpha2; Catenin alpha-2; catenin, alpha 1; Catenin-alpha1; Catenin-alpha2; Catna; Catna1; Catna2; cdf; cerebellar deficient folia; chp; CT114; CTNNA1; Ctnna2; CTNR; FLJ36832; FLJ52416; HGNC:2509; MDPT2; Neural alpha-catenin; NY-REN-13 antigen; renal carcinoma antigen NY-REN-13

more info or order :

Invitrogen product webpage