Bcl-2 Monoclonal Antibody (Bcl-2-100) | Invitrogen 13-8800 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Bcl-2 Monoclonal Antibody (Bcl-2-100)

catalog :

13-8800

quantity :

100 µg

price :

US 398.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Bcl-2-100

reactivity :

human, rat, chicken

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 51

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:250; loading ...; fig 1b	Ranjan K, Pathak C. Expression of FADD and cFLIPL balances mitochondrial integrity and redox signaling to substantiate apoptotic cell death. Mol Cell Biochem. 2016;422:135-150 pubmed
western blot; human; 1:500; fig 4f	Ranjan K, Pathak C. FADD regulates NF-ÎºB activation and promotes ubiquitination of cFLIPL to induce apoptosis. Sci Rep. 2016;6:22787 pubmed publisher
western blot; human; 1:1000; fig 3	Ranjan K, Pathak C. Expression of cFLIPL Determines the Basal Interaction of Bcl-2 With Beclin-1 and Regulates p53 Dependent Ubiquitination of Beclin-1 During Autophagic Stress. J Cell Biochem. 2016;117:1757-68 pubmed publisher
immunohistochemistry; human; 1:80; fig 5	Ertoy Baydar D, Kosemehmetoglu K, Aydin O, Bridge J, Buyukeren B, Aki F. Primary sclerosing epithelioid fibrosarcoma of kidney with variant histomorphologic features: report of 2 cases and review of the literature. Diagn Pathol. 2015;10:186 pubmed publisher
western blot; chicken; 1:1000; fig 7a	Luna Acosta J, Alba Betancourt C, MartÃnez Moreno C, RamÃrez C, Carranza M, Luna M, et al. Direct antiapoptotic effects of growth hormone are mediated by PI3K/Akt pathway in the chicken bursa of Fabricius. Gen Comp Endocrinol. 2015;224:148-59 pubmed publisher
immunohistochemistry - paraffin section; human; 1:200	Song M, Chung J, Lee J, Yang D, Kim I, Shin D, et al. High Ki-67 expression in involved bone marrow predicts worse clinical outcome in diffuse large B cell lymphoma patients treated with R-CHOP therapy. Int J Hematol. 2015;101:140-7 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100	Choi J, Kang S, Lee S, Bae Y. Prognostic significance of Bcl-2 expression in non-basal triple-negative breast cancer patients treated with anthracycline-based chemotherapy. Tumour Biol. 2014;35:12255-63 pubmed publisher
immunohistochemistry - paraffin section; human; 1:700	Nicholson A, Guo X, Sullivan C, Cha C. Automated quantitative analysis of tissue microarray of 443 patients with colorectal adenocarcinoma: low expression of Bcl-2 predicts poor survival. J Am Coll Surg. 2014;219:977-87 pubmed publisher
immunohistochemistry; rat; 1:200	Biaggio V, Alvarez Olmedo D, Pérez Chaca M, Salvetti N, Valdez S, Fanelli M, et al. Cytoprotective mechanisms in rats lung parenchyma with zinc deprivation. Biometals. 2014;27:305-15 pubmed publisher
immunohistochemistry - paraffin section; human; 1:50; fig 1	Razavi S, Hasheminia D, Mehdizade M, Movahedian B, Keshani F. The relation of pericoronal third molar follicle dimension and bcl-2/ki-67 expression: An immunohistochemical study. Dent Res J (Isfahan). 2012;9:S26-31 pubmed publisher
immunoprecipitation; human; fig 2 western blot; human; fig 2	Goraczniak R, Wall B, Behlke M, Lennox K, Ho E, Zaphiros N, et al. U1 Adaptor Oligonucleotides Targeting BCL2 and GRM1 Suppress Growth of Human Melanoma Xenografts In Vivo. Mol Ther Nucleic Acids. 2013;2:e92 pubmed publisher
immunoprecipitation; human; fig 9	Verma Y, Raghav P, Raj H, Tripathi R, Gangenahalli G. Enhanced heterodimerization of Bax by Bcl-2 mutants improves irradiated cell survival. Apoptosis. 2013;18:212-25 pubmed publisher
immunohistochemistry - paraffin section; human; fig 2	Giaginis C, Politi E, Alexandrou P, Sfiniadakis J, Kouraklis G, Theocharis S. Expression of peroxisome proliferator activated receptor-gamma (PPAR-?) in human non-small cell lung carcinoma: correlation with clinicopathological parameters, proliferation and apoptosis related molecules and patients' survival. Pathol Oncol Res. 2012;18:875-83 pubmed
western blot; human; 1:500	LÃºcio K, Rocha G, MonÃ§Ã£o Ribeiro L, Fernandes J, Takiya C, Gattass C. Oleanolic acid initiates apoptosis in non-small cell lung cancer cell lines and reduces metastasis of a B16F10 melanoma model in vivo. PLoS ONE. 2011;6:e28596 pubmed publisher
immunohistochemistry - paraffin section; human; 1:500; fig 1, 2, 3	Cheuk W, Tam F, Chan A, Luk I, Yuen A, Chan W, et al. Idiopathic cervical fibrosis--a new member of IgG4-related sclerosing diseases: report of 4 cases, 1 complicated by composite lymphoma. Am J Surg Pathol. 2010;34:1678-85 pubmed publisher
western blot; human; fig 4	Yang Z, von Ballmoos M, Faessler D, Voelzmann J, Ortmann J, Diehm N, et al. Paracrine factors secreted by endothelial progenitor cells prevent oxidative stress-induced apoptosis of mature endothelial cells. Atherosclerosis. 2010;211:103-9 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100; tbl 2	Jung C, Choi Y, Lee K, Bae J, Kim H, Yoon S, et al. The cytological, clinical, and pathological features of the cribriform-morular variant of papillary thyroid carcinoma and mutation analysis of CTNNB1 and BRAF genes. Thyroid. 2009;19:905-13 pubmed publisher
western blot; human	Thomson S, Cox A, Cuddihy S, Pullar J, Hampton M. Inhibition of receptor-mediated apoptosis upon Bcl-2 overexpression is not associated with increased antioxidant status. Biochem Biophys Res Commun. 2008;375:145-50 pubmed publisher
immunohistochemistry - paraffin section; human; 1:500	Cheuk W, Chan J, Nuovo G, Chan M, Fok M. Regression of gastric large B-Cell lymphoma accompanied by a florid lymphoma-like T-cell reaction: immunomodulatory effect of Ganoderma lucidum (Lingzhi)?. Int J Surg Pathol. 2007;15:180-6 pubmed
immunohistochemistry - paraffin section; human	Yoo J, Jung J, Lee M, Seo K, Shim B, Kim S, et al. Immunohistochemical analysis of non-small cell lung cancer: correlation with clinical parameters and prognosis. J Korean Med Sci. 2007;22:318-25 pubmed
immunohistochemistry - paraffin section; human; fig 2	Lawson J, Tran D. Localised breast cancers may have systemic influences on skin and hair. J Clin Pathol. 2007;60:180-4 pubmed
immunohistochemistry - paraffin section; human	Luo H, Yu S, Li T. Differential expression of apoptosis-related proteins in various cellular components of ameloblastomas. Int J Oral Maxillofac Surg. 2006;35:750-5 pubmed
immunohistochemistry - paraffin section; human; 1:200	Sun B, Sun Y, Wang J, Zhao X, Wang X, Hao X. Extent, relationship and prognostic significance of apoptosis and cell proliferation in synovial sarcoma. Eur J Cancer Prev. 2006;15:258-65 pubmed
immunocytochemistry; human; tbl 1	Rivadeneyra Espinoza L, Ruiz Argüelles A. Cell-penetrating anti-native DNA antibodies trigger apoptosis through both the neglect and programmed pathways. J Autoimmun. 2006;26:52-6 pubmed
immunohistochemistry; rat; tbl 1	Damron T, Horton J, Naqvi A, Margulies B, Strauss J, Grant W, et al. Decreased proliferation precedes growth factor changes after physeal irradiation. Clin Orthop Relat Res. 2004;:233-42 pubmed
western blot; human; 1:1000; fig 3	Sandalova E, Wei C, Masucci M, Levitsky V. Regulation of expression of Bcl-2 protein family member Bim by T cell receptor triggering. Proc Natl Acad Sci U S A. 2004;101:3011-6 pubmed
immunohistochemistry; rat; 1:100; fig 3	Damron T, Mathur S, Horton J, Strauss J, Margulies B, Grant W, et al. Temporal changes in PTHrP, Bcl-2, Bax, caspase, TGF-beta, and FGF-2 expression following growth plate irradiation with or without radioprotectant. J Histochem Cytochem. 2004;52:157-67 pubmed
immunohistochemistry - paraffin section; human; tbl 1	Ng J, Han A, Edelson M, Rosenblum N. Oncoprotein profiles of primary peritoneal malignant mixed müllerian tumors. Int J Gynecol Cancer. 2003;13:870-4 pubmed
immunohistochemistry - paraffin section; human; 1:50; tbl 2	Halperin R, Zehavi S, Hadas E, Habler L, Bukovsky I, Schneider D. Simultaneous carcinoma of the endometrium and ovary vs endometrial carcinoma with ovarian metastases: a clinical and immunohistochemical determination. Int J Gynecol Cancer. 2003;13:32-7 pubmed
immunohistochemistry - paraffin section; human	Tran D, Lawson J. Microcysts and breast cancer: a study of biological markers in archival biopsy material. Breast Cancer Res Treat. 2002;75:213-20 pubmed
immunohistochemistry; human; 1:20; tbl 3	Solomides C, Miller A, Christman R, Talwar J, Simpkins H. Lymphomas of the oral cavity: histology, immunologic type, and incidence of Epstein-Barr virus infection. Hum Pathol. 2002;33:153-7 pubmed
western blot; human; fig 5	Desai B, Myers B, Schreiber S. FKBP12-rapamycin-associated protein associates with mitochondria and senses osmotic stress via mitochondrial dysfunction. Proc Natl Acad Sci U S A. 2002;99:4319-24 pubmed
flow cytometry; human	Tanaka Y, Nomi M, Fujii K, Hubscher S, Maruo A, Matsumoto S, et al. Intercellular adhesion molecule 1 underlies the functional heterogeneity of synovial cells in patients with rheumatoid arthritis: involvement of cell cycle machinery. Arthritis Rheum. 2000;43:2513-22 pubmed
immunohistochemistry - paraffin section; human; 1:20; fig 2, 3, 4	Reis Filho J, Faoro L, Carrilho C, Bleggi Torres L, Schmitt F. Evaluation of cell proliferation, epidermal growth factor receptor, and bcl-2 immunoexpression as prognostic factors for patients with World Health Organization grade 2 oligodendroglioma. Cancer. 2000;88:862-9 pubmed
	Baltazar Lara R, Ávila Mendoza J, Martínez Moreno C, Carranza M, Pech Pool S, Vázquez Martínez O, et al. Neuroprotective Effects of Growth Hormone (GH) and Insulin-Like Growth Factor Type 1 (IGF-1) after Hypoxic-Ischemic Injury in Chicken Cerebellar Cell Cultures. Int J Mol Sci. 2020;22: pubmed publisher
	Masilamani A, Dettmer Monaco V, Monaco G, Cathomen T, Kuckuck I, Schultze Seemann S, et al. An Anti-PSMA Immunotoxin Reduces Mcl-1 and Bcl2A1 and Specifically Induces in Combination with the BAD-Like BH3 Mimetic ABT-737 Apoptosis in Prostate Cancer Cells. Cancers (Basel). 2020;12: pubmed publisher
	Li N, Cheng C, Wang T. MiR-181c-5p Mitigates Tumorigenesis in Cervical Squamous Cell Carcinoma via Targeting Glycogen Synthase Kinase 3β Interaction Protein (GSKIP). Onco Targets Ther. 2020;13:4495-4505 pubmed publisher
	Ji T, Feng W, Zhang X, Zang K, Zhu X, Shang F. HDAC inhibitors promote pancreatic stellate cell apoptosis and relieve pancreatic fibrosis by upregulating miR-15/16 in chronic pancreatitis. Hum Cell. 2020;33:1006-1016 pubmed publisher
	Ha Y, Fang Y, Romecin Duran P, Tolosa E, Moser C, Fernandez Zapico M, et al. Induction of Lysosome-associated Protein Transmembrane 4 Beta via Sulfatase 2 Enhances Autophagic Flux in Liver Cancer Cells. Hepatol Commun. 2019;3:1520-1543 pubmed publisher
	Kang Y, Anderson A, Jackson T, Palmer C, De Souza D, Fujihara K, et al. Function of hTim8a in complex IV assembly in neuronal cells provides insight into pathomechanism underlying Mohr-Tranebjærg syndrome. elife. 2019;8: pubmed publisher
	Ishikawa K, Kawano Y, Arihara Y, Kubo T, Takada K, Murase K, et al. BH3 profiling discriminates the anti‑apoptotic status of 5‑fluorouracil‑resistant colon cancer cells. Oncol Rep. 2019;42:2416-2425 pubmed publisher
	Lin J, Shen J, Yue H, Cao Z. miRNA‑183‑5p.1 promotes the migration and invasion of gastric cancer AGS cells by targeting TPM1. Oncol Rep. 2019;42:2371-2381 pubmed publisher
	Martínez Moreno C, Epardo D, Balderas Márquez J, Fleming T, Carranza M, Luna M, et al. Regenerative Effect of Growth Hormone (GH) in the Retina after Kainic Acid Excitotoxic Damage. Int J Mol Sci. 2019;20: pubmed publisher
	Saunders I, Mir H, Kapur N, Singh S. Emodin inhibits colon cancer by altering BCL-2 family proteins and cell survival pathways. Cancer Cell Int. 2019;19:98 pubmed publisher
	Mooney R, Majid A, Mota D, He A, Aramburo S, Flores L, et al. Bcl-2 Overexpression Improves Survival and Efficacy of Neural Stem Cell-Mediated Enzyme Prodrug Therapy. Stem Cells Int. 2018;2018:7047496 pubmed publisher
	Klein S, Abraham M, Bulvik B, Dery E, Weiss I, Barashi N, et al. CXCR4 Promotes Neuroblastoma Growth and Therapeutic Resistance through miR-15a/16-1-Mediated ERK and BCL2/Cyclin D1 Pathways. Cancer Res. 2018;78:1471-1483 pubmed publisher
	Cummins N, Sainski Nguyen A, Natesampillai S, Aboulnasr F, Kaufmann S, Badley A. Maintenance of the HIV Reservoir Is Antagonized by Selective BCL2 Inhibition. J Virol. 2017;91: pubmed publisher
	Shan T, Shan T, Liu F, Zheng H, Li G. Effects of Lycium barbarum polysaccharides on the damage to human endometrial stromal cells induced by hydrogen peroxide. Mol Med Rep. 2017;15:879-884 pubmed publisher
	Zhang S, Li Y, He X, Dong S, Huang Y, Li X, et al. Photothermolysis mediated by gold nanorods modified with EGFR monoclonal antibody induces Hep-2 cells apoptosis in vitro and in vivo. Int J Nanomedicine. 2014;9:1931-46 pubmed publisher
	Lecuit M, Nelson D, Smith S, Khun H, Huerre M, Vacher Lavenu M, et al. Targeting and crossing of the human maternofetal barrier by Listeria monocytogenes: role of internalin interaction with trophoblast E-cadherin. Proc Natl Acad Sci U S A. 2004;101:6152-7 pubmed
	Pullar J, Thomson S, King M, Turnbull C, Midwinter R, Hampton M. The chemopreventive agent phenethyl isothiocyanate sensitizes cells to Fas-mediated apoptosis. Carcinogenesis. 2004;25:765-72 pubmed

product information

Product Type :

Antibody

Product Name :

Bcl-2 Monoclonal Antibody (Bcl-2-100)

Catalog # :

13-8800

Quantity :

100 µg

Price :

US 398.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: Assay-dependent, Immunohistochemistry: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1 µg/mL

Species :

Human

Clone :

Bcl-2-100

Isotype :

IgG1, kappa

Storage :

-20°C

Description :

BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

Immunogen :

Synthetic peptide corresponding to residues 41-54 of the human Bcl-2 protein. This sequence is not shared by the mouse Bcl-2 protein.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-dependent, Immunohistochemistry: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1 µg/mL

Aliases :

apoptosis regulator Bcl-2; AW986256; B cell leukemia/lymphoma 2; B cell lymphoma 2 associated oncogene; B-cell CLL/lymphoma 2; B-cell leukemia/lymphoma 2; Bcl 2; BCL2; bcl-2; BCL2 apoptosis regulator; Bcl-2 protein; BCL2, apoptosis regulator; Bcl2-like protein; C430015F12Rik; D630044D05Rik; D830018M01Rik; OTTHUMP00000163680; PPP1R50; protein phosphatase 1, regulatory subunit 50

more info or order :

Invitrogen product webpage