Connexin 43 Monoclonal Antibody (CX-1B1) | Invitrogen 13-8300 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Connexin 43 Monoclonal Antibody (CX-1B1)

catalog :

13-8300

quantity :

100 µg

price :

US 482

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CX-1B1

reactivity :

hamsters, human, mouse, rat, dogs, bovine, pigs , domestic sheep, domestic rabbit

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section, blocking or activating experiments, mass cytometry

more info or order :

Invitrogen product webpage

citations: 129

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; rat; 1:100; loading ...; fig 4c	Wen S, Unuma K, Funakoshi T, Aki T, Uemura K. Contraction Band Necrosis with Dephosphorylated Connexin 43 in Rat Myocardium after Daily Cocaine Administration. Int J Mol Sci. 2022;23: pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:250; loading ...; fig 2a	Zhang P, Ishikawa M, Doyle A, Nakamura T, He B, Yamada Y. Pannexin 3 regulates skin development via Epiprofin. Sci Rep. 2021;11:1779 pubmed publisher
immunohistochemistry; rat; 1:200; loading ...; fig 8	Prado N, Muñoz E, Farias Altamirano L, Aguiar F, Ponce Zumino A, Sánchez F, et al. Reperfusion Arrhythmias Increase after Superior Cervical Ganglionectomy Due to Conduction Disorders and Changes in Repolarization. Int J Mol Sci. 2020;21: pubmed publisher
mass cytometry; mouse; loading ...; fig 5s2b	Dosch M, Zindel J, Jebbawi F, Melin N, Sánchez Taltavull D, Stroka D, et al. Connexin-43-dependent ATP release mediates macrophage activation during sepsis. elife. 2019;8: pubmed publisher
immunocytochemistry; mouse; 1:100; loading ...; fig 2a	Bondar C, Ormazábal M, Crivaro A, Ferreyra Compagnucci M, Delpino M, Rozenfeld P, et al. Osteocyte Alterations Induce Osteoclastogenesis in an In Vitro Model of Gaucher Disease. Int J Mol Sci. 2017;18: pubmed publisher
immunohistochemistry; rat; loading ...; fig 1	Dukic A, Haugen L, Pidoux G, Leithe E, Bakke O, Tasken K. A protein kinase A-ezrin complex regulates connexin 43 gap junction communication in liver epithelial cells. Cell Signal. 2017;32:1-11 pubmed publisher
immunohistochemistry - paraffin section; human; 1:2500; loading ...; fig 5c	Hamilton B, Woltjer R, Prola Netto J, Nesbit G, Gahramanov S, Pham T, et al. Ferumoxytol-enhanced MRI differentiation of meningioma from dural metastases: a pilot study with immunohistochemical observations. J Neurooncol. 2016;129:301-9 pubmed publisher
immunohistochemistry; mouse; loading ...; fig 6a	Forsberg D, Horn Z, Tserga E, Smedler E, Silberberg G, Shvarev Y, et al. CO2-evoked release of PGE2 modulates sighs and inspiration as demonstrated in brainstem organotypic culture. elife. 2016;5: pubmed publisher
immunohistochemistry; rat; fig 4	Boulaksil M, Bierhuizen M, Engelen M, Stein M, Kok B, van Amersfoorth S, et al. Spatial Heterogeneity of Cx43 is an Arrhythmogenic Substrate of Polymorphic Ventricular Tachycardias during Compensated Cardiac Hypertrophy in Rats. Front Cardiovasc Med. 2016;3:5 pubmed publisher
immunohistochemistry; mouse; 1:200	Pinet Charvet C, Geller S, Desroziers E, Ottogalli M, Lomet D, Georgelin C, et al. GnRH Episodic Secretion Is Altered by Pharmacological Blockade of Gap Junctions: Possible Involvement of Glial Cells. Endocrinology. 2016;157:304-22 pubmed publisher
western blot; domestic rabbit; fig 4	Zamudio A, Wang Z, Chung S, Wolosin J. Inhibition of TGFÎ² cell signaling for limbal explant culture in serumless, defined xeno-free conditions. Exp Eye Res. 2016;145:48-57 pubmed publisher
immunoprecipitation; human; fig 3 immunocytochemistry; human; fig 3 western blot; human; fig 3	Thuringer D, Berthenet K, Cronier L, Solary E, Garrido C. Primary tumor- and metastasis-derived colon cancer cells differently modulate connexin expression and function in human capillary endothelial cells. Oncotarget. 2015;6:28800-15 pubmed publisher
immunocytochemistry; human; 1:200; fig st1	Jones A, Gokhale P, Allison T, Sampson B, Athwal S, Grant S, et al. Evidence for bystander signalling between human trophoblast cells and human embryonic stem cells. Sci Rep. 2015;5:11694 pubmed publisher
immunohistochemistry; rat western blot; rat	Kandasamy K, Escue R, Manna J, Adebiyi A, Parthasarathi K. Changes in endothelial connexin 43 expression inversely correlate with microvessel permeability and VE-cadherin expression in endotoxin-challenged lungs. Am J Physiol Lung Cell Mol Physiol. 2015;309:L584-92 pubmed publisher
immunocytochemistry; mouse	Yang J, Kaur K, Ong L, Eisenberg C, Eisenberg L. Inhibition of G9a Histone Methyltransferase Converts Bone Marrow Mesenchymal Stem Cells to Cardiac Competent Progenitors. Stem Cells Int. 2015;2015:270428 pubmed publisher
western blot; human; 1:1000	Best M, Wu J, Pauli S, KANE M, Pierzchalski K, Session D, et al. A role for retinoids in human oocyte fertilization: regulation of connexin 43 by retinoic acid in cumulus granulosa cells. Mol Hum Reprod. 2015;21:527-34 pubmed publisher
immunocytochemistry; human; 1:100; fig 1	Rajnai H, Teleki I, Kiszner G, MeggyeshÃ¡zi N, Balla P, Vancsik T, et al. Connexin 43 communication channels in follicular dendritic cell development and in follicular lymphomas. J Immunol Res. 2015;2015:528098 pubmed publisher
immunohistochemistry; human	Lee J, Shahidullah M, Hotchkiss A, Coca Prados M, Delamere N, Pelis R. A renal-like organic anion transport system in the ciliary epithelium of the bovine and human eye. Mol Pharmacol. 2015;87:697-705 pubmed publisher
western blot; mouse; 1:500; fig s1	Chang K, Nayak R, Roy S, Perumbeti A, Wellendorf A, Bezold K, et al. Vasculopathy-associated hyperangiotensinemia mobilizes haematopoietic stem cells/progenitors through endothelial ATâ‚‚R and cytoskeletal dysregulation. Nat Commun. 2015;6:5914 pubmed publisher
western blot; human	Shriver M, Stroka K, Vitolo M, Martin S, Huso D, Konstantopoulos K, et al. Loss of giant obscurins from breast epithelium promotes epithelial-to-mesenchymal transition, tumorigenicity and metastasis. Oncogene. 2015;34:4248-59 pubmed publisher
western blot; rat; 1:1000	Kinjo E, Higa G, Morya E, Valle A, Kihara A, Britto L. Reciprocal regulation of epileptiform neuronal oscillations and electrical synapses in the rat hippocampus. PLoS ONE. 2014;9:e109149 pubmed publisher
blocking or activating experiments; human	Okamoto T, Akita N, Hayashi T, Shimaoka M, Suzuki K. Endothelial connexin 32 regulates tissue factor expression induced by inflammatory stimulation and direct cell-cell interaction with activated cells. Atherosclerosis. 2014;236:430-7 pubmed publisher
western blot; human; 0.5 ug/ml	Pidoux G, Gerbaud P, Dompierre J, Lygren B, Solstad T, Evain Brion D, et al. A PKA-ezrin-Cx43 signaling complex controls gap junction communication and thereby trophoblast cell fusion. J Cell Sci. 2014;127:4172-85 pubmed publisher
western blot; domestic sheep	Sadowska G, Stonestreet B. Maternal treatment with glucocorticoids modulates gap junction protein expression in the ovine fetal brain. Neuroscience. 2014;275:248-58 pubmed publisher
immunohistochemistry; human; 1:400; loading ...; fig 5c	Gevaert T, Vanstreels E, Daelemans D, Franken J, Van Der Aa F, Roskams T, et al. Identification of different phenotypes of interstitial cells in the upper and deep lamina propria of the human bladder dome. J Urol. 2014;192:1555-63 pubmed publisher
western blot; rat; loading ...; fig 6b	Kudo Sakamoto Y, Akazawa H, Ito K, Takano J, Yano M, Yabumoto C, et al. Calpain-dependent cleavage of N-cadherin is involved in the progression of post-myocardial infarction remodeling. J Biol Chem. 2014;289:19408-19 pubmed publisher
immunohistochemistry - frozen section; human; fig 4b western blot; human; fig 6a	Förster T, Rausch V, Zhang Y, Isayev O, Heilmann K, Schoensiegel F, et al. Sulforaphane counteracts aggressiveness of pancreatic cancer driven by dysregulated Cx43-mediated gap junctional intercellular communication. Oncotarget. 2014;5:1621-34 pubmed
western blot; mouse; 1:500	Bejarano E, Yuste A, Patel B, Stout R, Spray D, Cuervo A. Connexins modulate autophagosome biogenesis. Nat Cell Biol. 2014;16:401-14 pubmed publisher
immunocytochemistry; mouse western blot; mouse	Solan J, Lampe P. Specific Cx43 phosphorylation events regulate gap junction turnover in vivo. FEBS Lett. 2014;588:1423-9 pubmed publisher
immunocytochemistry; mouse immunocytochemistry; rat	Bautista W, Rash J, Vanderpool K, Yasumura T, Nagy J. Re-evaluation of connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus. Eur J Neurosci. 2014;39:757-70 pubmed publisher
western blot; human; 1:1000	Yu J, Boicea A, Barrett K, James C, Bagchi I, Bagchi M, et al. Reduced connexin 43 in eutopic endometrium and cultured endometrial stromal cells from subjects with endometriosis. Mol Hum Reprod. 2014;20:260-70 pubmed publisher
immunocytochemistry; human; 1:200	Glenske K, Wagner A, Hanke T, Cavalcanti Adam E, Heinemann S, Heinemann C, et al. Bioactivity of xerogels as modulators of osteoclastogenesis mediated by connexin 43. Biomaterials. 2014;35:1487-95 pubmed publisher
immunohistochemistry; human; 1:100	Liu W, Glueckert R, Linthicum F, Rieger G, Blumer M, Bitsche M, et al. Possible role of gap junction intercellular channels and connexin 43 in satellite glial cells (SGCs) for preservation of human spiral ganglion neurons : A comparative study with clinical implications. Cell Tissue Res. 2014;355:267-78 pubmed publisher
western blot; human; 1:2000; fig 2	Fong J, Kells R, Falk M. Two tyrosine-based sorting signals in the Cx43 C-terminus cooperate to mediate gap junction endocytosis. Mol Biol Cell. 2013;24:2834-48 pubmed publisher
western blot; rat; 3 ug/ml; fig 2	Novo R, Freire C, Felisbino S, Minicucci M, Azevedo P, Zornoff L, et al. Smoking is associated with remodeling of gap junction in the rat heart: smoker's paradox explanation?. Arq Bras Cardiol. 2013;100:274-80 pubmed
immunohistochemistry - frozen section; human; 1:50; fig 6	Takahashi M, Tajika Y, Khairani A, Ueno H, Murakami T, Yorifuji H. The localization of VAMP5 in skeletal and cardiac muscle. Histochem Cell Biol. 2013;139:573-82 pubmed publisher
western blot; human; 1:1000; fig 1	Wu J, Taylor R, Sidell N. Retinoic acid regulates gap junction intercellular communication in human endometrial stromal cells through modulation of the phosphorylation status of connexin 43. J Cell Physiol. 2013;228:903-10 pubmed publisher
western blot; rat; fig 1	Wu W, Li Y, Lu Z, Hu X. Increased susceptibility to ischemia-induced ventricular tachyarrhythmias in depressed rats: Involvement of reduction of connexin 43. Exp Ther Med. 2012;3:192-194 pubmed
western blot; mouse; fig 1	Swope D, Cheng L, Gao E, Li J, Radice G. Loss of cadherin-binding proteins ?-catenin and plakoglobin in the heart leads to gap junction remodeling and arrhythmogenesis. Mol Cell Biol. 2012;32:1056-67 pubmed publisher
western blot; mouse; fig 1	Marquez Rosado L, Solan J, Dunn C, Norris R, Lampe P. Connexin43 phosphorylation in brain, cardiac, endothelial and epithelial tissues. Biochim Biophys Acta. 2012;1818:1985-92 pubmed publisher
western blot; rat; fig 3	Stauffer B, Sobus R, Sucharov C. Sex differences in cardiomyocyte connexin43 expression. J Cardiovasc Pharmacol. 2011;58:32-9 pubmed publisher
western blot; human; 1:250; fig 4	Luckprom P, Kanjanamekanant K, Pavasant P. Role of connexin43 hemichannels in mechanical stress-induced ATP release in human periodontal ligament cells. J Periodontal Res. 2011;46:607-15 pubmed publisher
immunohistochemistry; human; 1:100; tbl 3	Chui J, Coroneo M, Tat L, Crouch R, Wakefield D, Di Girolamo N. Ophthalmic pterygium: a stem cell disorder with premalignant features. Am J Pathol. 2011;178:817-27 pubmed publisher
immunocytochemistry; human; 1:100; fig 3	Magnotti L, Goodenough D, Paul D. Functional heterotypic interactions between astrocyte and oligodendrocyte connexins. Glia. 2011;59:26-34 pubmed publisher
immunohistochemistry; dogs; 1:500; fig 4	Hussain W, Patel P, Chowdhury R, Cabo C, Ciaccio E, Lab M, et al. The Renin-Angiotensin system mediates the effects of stretch on conduction velocity, connexin43 expression, and redistribution in intact ventricle. J Cardiovasc Electrophysiol. 2010;21:1276-83 pubmed publisher
immunohistochemistry - frozen section; rat; 10 ug/ml; fig 1	Mühlfeld C, Cetegen C, Freese S, Volkmann R, Hellige G, Vetterlein F. Phosphorylation of extrajunctional Cx43 in ischemic-preconditioned rat hearts. J Surg Res. 2010;162:e1-8 pubmed publisher
flow cytometry; human; fig 2	Oviedo Orta E, Perreau M, Evans W, Potolicchio I. Control of the proliferation of activated CD4+ T cells by connexins. J Leukoc Biol. 2010;88:79-86 pubmed publisher
western blot; rat; fig 2	Girao H, Catarino S, Pereira P. Eps15 interacts with ubiquitinated Cx43 and mediates its internalization. Exp Cell Res. 2009;315:3587-97 pubmed publisher
western blot; rat; fig 1	Shintani Ishida K, Unuma K, Yoshida K. Ischemia enhances translocation of connexin43 and gap junction intercellular communication, thereby propagating contraction band necrosis after reperfusion. Circ J. 2009;73:1661-8 pubmed
immunohistochemistry - frozen section; human; fig 7b western blot; human; loading ...; fig 1c immunohistochemistry - frozen section; mouse; loading ...; fig 5 western blot; mouse; loading ...; fig 7a	Ey B, Eyking A, Gerken G, Podolsky D, Cario E. TLR2 mediates gap junctional intercellular communication through connexin-43 in intestinal epithelial barrier injury. J Biol Chem. 2009;284:22332-43 pubmed publisher
immunohistochemistry - frozen section; rat; 1:500; fig 1	Beraneck M, Uno A, Vassias I, Idoux E, de Waele C, Vidal P, et al. Evidence against a role of gap junctions in vestibular compensation. Neurosci Lett. 2009;450:97-101 pubmed publisher
immunohistochemistry - frozen section; bovine; 1:100; fig 5	Berisha B, Bridger P, Toth A, Kliem H, Meyer H, Schams D, et al. Expression and localization of gap junctional connexins 26 and 43 in bovine periovulatory follicles and in corpus luteum during different functional stages of oestrous cycle and pregnancy. Reprod Domest Anim. 2009;44:295-302 pubmed publisher
immunocytochemistry; rat	de Diego C, Pai R, Chen F, Xie L, de Leeuw J, Weiss J, et al. Electrophysiological consequences of acute regional ischemia/reperfusion in neonatal rat ventricular myocyte monolayers. Circulation. 2008;118:2330-7 pubmed publisher
western blot; human; 1:500	Serre Beinier V, Bosco D, Zulianello L, Charollais A, Caille D, Charpantier E, et al. Cx36 makes channels coupling human pancreatic beta-cells, and correlates with insulin expression. Hum Mol Genet. 2009;18:428-39 pubmed publisher
flow cytometry; human; fig 2 western blot; human; fig 1	Li Z, Zhou Z, Welch D, Donahue H. Expressing connexin 43 in breast cancer cells reduces their metastasis to lungs. Clin Exp Metastasis. 2008;25:893-901 pubmed publisher
immunohistochemistry - paraffin section; pigs ; 1:100; fig 2 western blot; pigs ; 1:200; fig 3a	Totzeck A, Boengler K, van De Sand A, Konietzka I, Gres P, Garcia Dorado D, et al. No impact of protein phosphatases on connexin 43 phosphorylation in ischemic preconditioning. Am J Physiol Heart Circ Physiol. 2008;295:H2106-12 pubmed publisher
immunohistochemistry - frozen section; human; 1:100; fig 2	Hutchings G, Gevaert T, Deprest J, Roskams T, Van Lommel A, Nilius B, et al. Immunohistochemistry using an antibody to unphosphorylated connexin 43 to identify human myometrial interstitial cells. Reprod Biol Endocrinol. 2008;6:43 pubmed publisher
immunocytochemistry; rat; 1:50; fig 5	Liang J, Wang S, Chung T, Yang S, Wu J. Effects of 18-glycyrrhetinic acid on serine 368 phosphorylation of connexin43 in rat neonatal cardiomyocytes. Cell Biol Int. 2008;32:1371-9 pubmed publisher
western blot; mouse; fig 6	ElZarrad M, Haroon A, Willecke K, Dobrowolski R, Gillespie M, Al Mehdi A. Connexin-43 upregulation in micrometastases and tumor vasculature and its role in tumor cell attachment to pulmonary endothelium. BMC Med. 2008;6:20 pubmed publisher
immunocytochemistry; rat; fig 2	Kang J, Kang N, Lovatt D, Torres A, Zhao Z, Lin J, et al. Connexin 43 hemichannels are permeable to ATP. J Neurosci. 2008;28:4702-11 pubmed publisher
western blot; mouse; fig 1	Li J, Levin M, Xiong Y, Petrenko N, Patel V, Radice G. N-cadherin haploinsufficiency affects cardiac gap junctions and arrhythmic susceptibility. J Mol Cell Cardiol. 2008;44:597-606 pubmed publisher
western blot; rat; fig 2	Sundset R, Ytrehus K, Zhang Y, Saffitz J, Yamada K. Repeated simulated ischemia and protection against gap junctional uncoupling. Cell Commun Adhes. 2007;14:239-49 pubmed
immunocytochemistry; human; fig 4 western blot; human; fig 3a	Leinonen P, Aaltonen V, Koskela S, Lehenkari P, Korkiamäki T, Peltonen J. Impaired gap junction formation and intercellular calcium signaling in urinary bladder cancer cells can be improved by Gö6976. Cell Commun Adhes. 2007;14:125-36 pubmed
immunocytochemistry; human; fig 2 western blot; human; fig 2	Meilleur M, Akpovi C, Pelletier R, Vitale M. Tumor necrosis factor-alpha-induced anterior pituitary folliculostellate TtT/GF cell uncoupling is mediated by connexin 43 dephosphorylation. Endocrinology. 2007;148:5913-24 pubmed
immunocytochemistry; rat; 1:500	Zhang S, Liu Y, Huang G, Liu L. Aconitine alters connexin43 phosphorylation status and [Ca2+] oscillation patterns in cultured ventricular myocytes of neonatal rats. Toxicol In Vitro. 2007;21:1476-85 pubmed
immunohistochemistry - frozen section; rat; 1:100 western blot; rat; 1:1000	Wang R, Zhang C, Ruan Y, Liu N, Wang L. Changes in phosphorylation of connexin43 in rats during acute myocardial hypoxia and effects of antiarrhythmic peptide on the phosphorylation. J Huazhong Univ Sci Technolog Med Sci. 2007;27:241-4 pubmed
immunocytochemistry; human; fig st1 western blot; human; 1:500; fig st1	Malone P, Miao H, Parker A, Juarez S, Hernandez M. Pressure induces loss of gap junction communication and redistribution of connexin 43 in astrocytes. Glia. 2007;55:1085-98 pubmed
western blot; human western blot; rat	Girao H, Pereira P. The proteasome regulates the interaction between Cx43 and ZO-1. J Cell Biochem. 2007;102:719-28 pubmed
immunohistochemistry; rat; 1:200; fig 1 western blot; rat; 1:1000; fig 1	Goubaeva F, Mikami M, Giardina S, Ding B, Abe J, Yang J. Cardiac mitochondrial connexin 43 regulates apoptosis. Biochem Biophys Res Commun. 2007;352:97-103 pubmed
immunohistochemistry; rat; 1:50; fig 2 western blot; rat; 1:250; fig 3	Chung T, Wang S, Chang Y, Chen Y, Wu J. 18beta-glycyrrhetinic acid promotes src interaction with connexin43 in rat cardiomyocytes. J Cell Biochem. 2007;100:653-64 pubmed
immunohistochemistry; human; 1:10; fig 1 western blot; human; 1:20; fig 1 immunohistochemistry; rat; 1:10; fig 2 western blot; rat; 1:20; fig 2	Rucker Martin C, Milliez P, Tan S, Decrouy X, Recouvreur M, Vranckx R, et al. Chronic hemodynamic overload of the atria is an important factor for gap junction remodeling in human and rat hearts. Cardiovasc Res. 2006;72:69-79 pubmed
immunocytochemistry; dogs	Larre I, Ponce A, Fiorentino R, Shoshani L, Contreras R, Cereijido M. Contacts and cooperation between cells depend on the hormone ouabain. Proc Natl Acad Sci U S A. 2006;103:10911-6 pubmed
western blot; rat; fig 1	Choi Y, Stamm C, Hammer P, Kwaku K, Marler J, Friehs I, et al. Cardiac conduction through engineered tissue. Am J Pathol. 2006;169:72-85 pubmed
immunocytochemistry; mouse; 1:500; fig 5 western blot; mouse; 1:250; fig 5	Rouach N, Pebay A, Meme W, Cordier J, Ezan P, Etienne E, et al. S1P inhibits gap junctions in astrocytes: involvement of G and Rho GTPase/ROCK. Eur J Neurosci. 2006;23:1453-64 pubmed
immunohistochemistry - paraffin section; mouse; loading ...; fig 1m	Lee M, Kim J, Lee S, Sasaki H, Lunny D, Lane E, et al. Association of Shh and Ptc with keratin localization in the initiation of the formation of circumvallate papilla and von Ebner's gland. Cell Tissue Res. 2006;325:253-61 pubmed
immunohistochemistry - frozen section; domestic rabbit; 1:200	Ripplinger C, Krinsky V, Nikolski V, Efimov I. Mechanisms of unpinning and termination of ventricular tachycardia. Am J Physiol Heart Circ Physiol. 2006;291:H184-92 pubmed
flow cytometry; human; fig 2 immunocytochemistry; human; 1:50; fig 1	Chen Z, Evans W, Pflugfelder S, Li D. Gap junction protein connexin 43 serves as a negative marker for a stem cell-containing population of human limbal epithelial cells. Stem Cells. 2006;24:1265-73 pubmed
immunocytochemistry; rat; 1:200; loading ...; fig 1e western blot; rat; loading ...; fig 2a	Gorbe A, Becker D, Dux L, Krenacs L, Krenacs T. In differentiating prefusion myoblasts connexin43 gap junction coupling is upregulated before myoblast alignment then reduced in post-mitotic cells. Histochem Cell Biol. 2006;125:705-16 pubmed
western blot; rat; fig 5	Sundset R, Cooper M, Mikalsen S, Ytrehus K. Ischemic preconditioning protects against gap junctional uncoupling in cardiac myofibroblasts. Cell Commun Adhes. 2004;11:51-66 pubmed
immunohistochemistry - paraffin section; mouse	King T, Lampe P. Temporal regulation of connexin phosphorylation in embryonic and adult tissues. Biochim Biophys Acta. 2005;1719:24-35 pubmed
immunohistochemistry - frozen section; rat; 1:100; fig 1	Gorbe A, Becker D, Dux L, Stelkovics E, Krenacs L, Bagdi E, et al. Transient upregulation of connexin43 gap junctions and synchronized cell cycle control precede myoblast fusion in regenerating skeletal muscle in vivo. Histochem Cell Biol. 2005;123:573-83 pubmed
immunohistochemistry - paraffin section; mouse	Kim J, Cho S, Lee M, Hwang H, Lee J, Lee S, et al. Inhibition of connexin 43 alters Shh and Bmp-2 expression patterns in embryonic mouse tongue. Cell Tissue Res. 2005;320:409-15 pubmed
western blot; rat; 1:500	Leite A, Carvalho C, Furtado A, Barbosa H, Boschero A, Collares Buzato C. Co-expression and regulation of connexins 36 and 43 in cultured neonatal rat pancreatic islets. Can J Physiol Pharmacol. 2005;83:142-51 pubmed
immunocytochemistry; mouse; fig 2 western blot; mouse; fig 1	Li W, Hertzberg E, Spray D. Regulation of connexin43-protein binding in astrocytes in response to chemical ischemia/hypoxia. J Biol Chem. 2005;280:7941-8 pubmed
immunocytochemistry; rat; 1:50 western blot; rat; 1:250	Chung T, Wang S, Wu J. 17beta-estradiol reduces the effect of metabolic inhibition on gap junction intercellular communication in rat cardiomyocytes via the estrogen receptor. J Mol Cell Cardiol. 2004;37:1013-22 pubmed
immunohistochemistry - frozen section; mouse; 1:100	Altevogt B, Paul D. Four classes of intercellular channels between glial cells in the CNS. J Neurosci. 2004;24:4313-23 pubmed
immunocytochemistry; hamsters; 1:100; fig 9	Cruciani V, Leithe E, Mikalsen S. Ilimaquinone inhibits gap-junctional communication prior to Golgi fragmentation and block in protein transport. Exp Cell Res. 2003;287:130-42 pubmed
immunohistochemistry; rat; 10 ug/ml	Zahs K, Kofuji P, Meier C, Dermietzel R. Connexin immunoreactivity in glial cells of the rat retina. J Comp Neurol. 2003;455:531-46 pubmed
western blot; rat; 1:600	Jahromi S, Wentlandt K, Piran S, Carlen P. Anticonvulsant actions of gap junctional blockers in an in vitro seizure model. J Neurophysiol. 2002;88:1893-902 pubmed
immunocytochemistry; human; 1:10; fig 6	Rucker Martin C, Pecker F, Godreau D, Hatem S. Dedifferentiation of atrial myocytes during atrial fibrillation: role of fibroblast proliferation in vitro. Cardiovasc Res. 2002;55:38-52 pubmed
immunocytochemistry; mouse western blot; mouse	Bruzzone S, Franco L, Guida L, Zocchi E, Contini P, Bisso A, et al. A self-restricted CD38-connexin 43 cross-talk affects NAD+ and cyclic ADP-ribose metabolism and regulates intracellular calcium in 3T3 fibroblasts. J Biol Chem. 2001;276:48300-8 pubmed
western blot; rat; fig 2	Verderio C, Bruzzone S, Zocchi E, Fedele E, Schenk U, De Flora A, et al. Evidence of a role for cyclic ADP-ribose in calcium signalling and neurotransmitter release in cultured astrocytes. J Neurochem. 2001;78:646-57 pubmed
immunohistochemistry - frozen section; human; 1:100	Hentula M, Peltonen J, Peltonen S. Expression profiles of cell-cell and cell-matrix junction proteins in developing human epidermis. Arch Dermatol Res. 2001;293:259-67 pubmed
immunocytochemistry; rat; 1:1000; fig 4	Mercier F, Hatton G. Connexin 26 and basic fibroblast growth factor are expressed primarily in the subpial and subependymal layers in adult brain parenchyma: roles in stem cell proliferation and morphological plasticity?. J Comp Neurol. 2001;431:88-104 pubmed
immunohistochemistry - free floating section; rat; fig 2	Nagy J, Li W. A brain slice model for in vitro analyses of astrocytic gap junction and connexin43 regulation: actions of ischemia, glutamate and elevated potassium. Eur J Neurosci. 2000;12:4567-72 pubmed
immunocytochemistry; rat; 1:500 western blot; rat; 1:750	Li W, Nagy J. Connexin43 phosphorylation state and intercellular communication in cultured astrocytes following hypoxia and protein phosphatase inhibition. Eur J Neurosci. 2000;12:2644-50 pubmed
immunohistochemistry; rat; 1:500; fig 1 western blot; rat; 1:750; fig 7	Li W, Nagy J. Activation of fibres in rat sciatic nerve alters phosphorylation state of connexin-43 at astrocytic gap junctions in spinal cord: evidence for junction regulation by neuronal-glial interactions. Neuroscience. 2000;97:113-23 pubmed
western blot; mouse; fig 1	Cruciani V, Mikalsen S. Stimulated phosphorylation of intracellular connexin43. Exp Cell Res. 1999;251:285-98 pubmed
immunohistochemistry - frozen section; rat; 1:500; fig 15	Nagy J, Patel D, Ochalski P, Stelmack G. Connexin30 in rodent, cat and human brain: selective expression in gray matter astrocytes, co-localization with connexin43 at gap junctions and late developmental appearance. Neuroscience. 1999;88:447-68 pubmed
immunohistochemistry - frozen section; human	Krenacs T, Rosendaal M. Gap-junction communication pathways in germinal center reactions. Dev Immunol. 1998;6:111-8 pubmed
	Chang J, Cha M, Seo J, Kim H, Park S, Kim B, et al. Feasibility study on stereotactic radiotherapy for total pulmonary vein isolation in a canine model. Sci Rep. 2021;11:12369 pubmed publisher
	Gasiūnienė M, Valatkaitė E, Navakauskaitė A, Navakauskiene R. The Effect of Angiotensin II, Retinoic Acid, EGCG, and Vitamin C on the Cardiomyogenic Differentiation Induction of Human Amniotic Fluid-Derived Mesenchymal Stem Cells. Int J Mol Sci. 2020;21: pubmed publisher
	Yu J, Berga S, Zou W, Taylor R. Interleukin-1β inhibits estrogen receptor-α, progesterone receptors A and B and biomarkers of human endometrial stromal cell differentiation: implications for endometriosis. Mol Hum Reprod. 2019;25:625-637 pubmed publisher
	Fan X, Bialecka M, Moustakas I, Lam E, Torrens Juaneda V, Borggreven N, et al. Single-cell reconstruction of follicular remodeling in the human adult ovary. Nat Commun. 2019;10:3164 pubmed publisher
	Kim E, Bae Y, Choi M, Hong S. Connexin 43 plays an important role in the transformation of cholangiocytes with Clonochis sinensis excretory-secretory protein and N-nitrosodimethylamine. PLoS Negl Trop Dis. 2019;13:e0006843 pubmed publisher
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product information

Product Type :

Antibody

Product Name :

Connexin 43 Monoclonal Antibody (CX-1B1)

Catalog # :

13-8300

Quantity :

100 µg

Price :

US 482

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Canine, Human, Mouse, Rat

Applications :

ELISA: 0.1-1 µg/mL, Immunohistochemistry (Paraffin): 1:10-1:100, Immunomicroscopy: Assay-dependent, Western Blot: 1-3 µg/mL

Species :

Canine, Human, Mouse, Rat

Clone :

CX-1B1

Isotype :

IgG1

Storage :

-20°C

Description :

Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication. GFAP are membrane-spanning proteins that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is also targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5. Mutations in the GFAP gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy, oculodentodigital dysplasia and heart malformations. Alternatively spliced transcript variants of GFAP have been found.

Immunogen :

Synthetic peptide corresponding to a cytoplasmic sequence located near the C-terminus of rat Connexin 43.

Format :

Liquid

Applications w/Dilutions :

ELISA: 0.1-1 µg/mL, Immunohistochemistry (Paraffin): 1:10-1:100, Immunomicroscopy: Assay-dependent, Western Blot: 1-3 µg/mL

Aliases :

alpha 1 connexin; AU042049; AVSD3; AW546267; CMDR; Cnx43; connexin 43; connexin43; connexin-43; cx43; Cx-43; cx43 protein; cx43a1; Cx43alpha1; CXA1; Cxn-43; DFNB38; EKVP; etID309742.9; gap junction 43 kDa heart protein; Gap junction alpha-1 protein; gap junction membrane channel protein; gap junction membrane channel protein alpha 1; gap junction protein; gap junction protein alpha 1; gap junction protein connexin 43; gap junction protein, alpha 1; gap junction protein, alpha 1, 43 kD (connexin 43); gap junction protein, alpha 1, 43kDa; gap junction protein, alpha 1, 43kDa (connexin 43); gja1; Gja-1; GJAL; HLHS1; HSS; Npm1; ODD; ODDD; ODOD; PPKCA; SDTY3; shf; Short fin protein; sof; stopsel; stp; Syndactyly type III; vascular smooth muscle connexin43; Vascular smooth muscle connexin-43; zfCx43.3

more info or order :

Invitrogen product webpage