Connexin 32 Monoclonal Antibody (CX-2C2) | Invitrogen 13-8200 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Connexin 32 Monoclonal Antibody (CX-2C2)

catalog :

13-8200

quantity :

100 µg

price :

US 406.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CX-2C2

reactivity :

human, mouse, rat, dogs, domestic sheep

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, blocking or activating experiments, western blot knockout validation, immunocytochemistry knockout validation

more info or order :

Invitrogen product webpage

citations: 35

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry knockout validation; mouse; 1.0 ug/ml; fig 4 western blot knockout validation; mouse; 1:250; fig 2, 3	Fowler S, McLean A, Bennett S. Tissue-specific cross-reactivity of connexin32 antibodies: problems and solutions unique to the central nervous system. Cell Commun Adhes. 2009;16:117-30 pubmed publisher
immunocytochemistry; human; loading ...; fig 7	Mattei G, Magliaro C, Giusti S, Ramachandran S, Heinz S, Braspenning J, et al. On the adhesion-cohesion balance and oxygen consumption characteristics of liver organoids. PLoS ONE. 2017;12:e0173206 pubmed publisher
western blot; mouse; 1:500; fig 2	Ramani M, Mylvaganam S, Krawczyk M, Wang L, Zoidl C, Brien J, et al. Differential expression of astrocytic connexins in a mouse model of prenatal alcohol exposure. Neurobiol Dis. 2016;91:83-93 pubmed publisher
immunocytochemistry; human; fig 4 western blot; human; fig 4	Thuringer D, Berthenet K, Cronier L, Solary E, Garrido C. Primary tumor- and metastasis-derived colon cancer cells differently modulate connexin expression and function in human capillary endothelial cells. Oncotarget. 2015;6:28800-15 pubmed publisher
western blot; human; 0.5 ug/ml	Slusser A, Bathula C, Sens D, Somji S, Sens M, Zhou X, et al. Cadherin expression, vectorial active transport, and metallothionein isoform 3 mediated EMT/MET responses in cultured primary and immortalized human proximal tubule cells. PLoS ONE. 2015;10:e0120132 pubmed publisher
blocking or activating experiments; human	Okamoto T, Akita N, Hayashi T, Shimaoka M, Suzuki K. Endothelial connexin 32 regulates tissue factor expression induced by inflammatory stimulation and direct cell-cell interaction with activated cells. Atherosclerosis. 2014;236:430-7 pubmed publisher
western blot; domestic sheep	Sadowska G, Stonestreet B. Maternal treatment with glucocorticoids modulates gap junction protein expression in the ovine fetal brain. Neuroscience. 2014;275:248-58 pubmed publisher
western blot; human; 1:500	Yu J, Boicea A, Barrett K, James C, Bagchi I, Bagchi M, et al. Reduced connexin 43 in eutopic endometrium and cultured endometrial stromal cells from subjects with endometriosis. Mol Hum Reprod. 2014;20:260-70 pubmed publisher
western blot; mouse; 1:500; fig 1	Mayes D, Rizvi T, Titus Mitchell H, Oberst R, Ciraolo G, Vorhees C, et al. Nf1 loss and Ras hyperactivation in oligodendrocytes induce NOS-driven defects in myelin and vasculature. Cell Rep. 2013;4:1197-212 pubmed publisher
western blot; mouse; fig s1	Fowler S, Akins M, Zhou H, Figeys D, Bennett S. The liver connexin32 interactome is a novel plasma membrane-mitochondrial signaling nexus. J Proteome Res. 2013;12:2597-610 pubmed publisher
western blot; mouse; 1:500; fig 4	Degen J, Schütz M, Dicke N, Strenzke N, Jokwitz M, Moser T, et al. Connexin32 can restore hearing in connexin26 deficient mice. Eur J Cell Biol. 2011;90:817-24 pubmed publisher
immunocytochemistry; rat; 1:100; fig 3	Takeuchi A, Fukazawa S, Chida K, Taguchi M, Shirataka M, Ikeda N. Semi-automatic counting of connexin 32s immunolocalized in cultured fetal rat hepatocytes using image processing. Acta Histochem. 2012;114:318-26 pubmed publisher
immunocytochemistry; human; 1:100; fig 3	Magnotti L, Goodenough D, Paul D. Functional heterotypic interactions between astrocyte and oligodendrocyte connexins. Glia. 2011;59:26-34 pubmed publisher
immunohistochemistry - frozen section; human; 1:200; fig 1	Kawasaki Y, Omori Y, Li Q, Nishikawa Y, Yoshioka T, Yoshida M, et al. Cytoplasmic accumulation of connexin32 expands cancer stem cell population in human HuH7 hepatoma cells by enhancing its self-renewal. Int J Cancer. 2011;128:51-62 pubmed publisher
immunocytochemistry; human; 1:500	Okamoto T, Akiyama M, Takeda M, Gabazza E, Hayashi T, Suzuki K. Connexin32 is expressed in vascular endothelial cells and participates in gap-junction intercellular communication. Biochem Biophys Res Commun. 2009;382:264-8 pubmed publisher
immunocytochemistry; human; fig 4 western blot; human; fig 3a	Leinonen P, Aaltonen V, Koskela S, Lehenkari P, Korkiamäki T, Peltonen J. Impaired gap junction formation and intercellular calcium signaling in urinary bladder cancer cells can be improved by Gö6976. Cell Commun Adhes. 2007;14:125-36 pubmed
immunocytochemistry; human; 1:500 immunohistochemistry; human; 1:200	Li Q, Omori Y, Nishikawa Y, Yoshioka T, Yamamoto Y, Enomoto K. Cytoplasmic accumulation of connexin32 protein enhances motility and metastatic ability of human hepatoma cells in vitro and in vivo. Int J Cancer. 2007;121:536-46 pubmed
western blot; human; 1:500; fig 6	Page J, Johnson M, Olsavsky K, Strom S, Zarbl H, Omiecinski C. Gene expression profiling of extracellular matrix as an effector of human hepatocyte phenotype in primary cell culture. Toxicol Sci. 2007;97:384-97 pubmed
western blot; human; 1:500	Abbaci M, Barberi Heyob M, Stines J, Blondel W, Dumas D, Guillemin F, et al. Gap junctional intercellular communication capacity by gap-FRAP technique: a comparative study. Biotechnol J. 2007;2:50-61 pubmed
immunocytochemistry; dogs western blot; dogs	Larre I, Ponce A, Fiorentino R, Shoshani L, Contreras R, Cereijido M. Contacts and cooperation between cells depend on the hormone ouabain. Proc Natl Acad Sci U S A. 2006;103:10911-6 pubmed
immunohistochemistry - paraffin section; human; 1:500	Kubota T, Sato K, Arishima H, Takeuchi H, Kitai R, Nakagawa T. Astroblastoma: immunohistochemical and ultrastructural study of distinctive epithelial and probable tanycytic differentiation. Neuropathology. 2006;26:72-81 pubmed
immunocytochemistry; human; 1:50; fig 2	Chen Z, Evans W, Pflugfelder S, Li D. Gap junction protein connexin 43 serves as a negative marker for a stem cell-containing population of human limbal epithelial cells. Stem Cells. 2006;24:1265-73 pubmed
immunohistochemistry - frozen section; rat; 1:100	Gorbe A, Becker D, Dux L, Stelkovics E, Krenacs L, Bagdi E, et al. Transient upregulation of connexin43 gap junctions and synchronized cell cycle control precede myoblast fusion in regenerating skeletal muscle in vivo. Histochem Cell Biol. 2005;123:573-83 pubmed
immunocytochemistry; human; 1:500 western blot; human; 1:500; fig 5	Saito T, Tanaka R, Wataba K, Kudo R, Yamasaki H. Overexpression of estrogen receptor-alpha gene suppresses gap junctional intercellular communication in endometrial carcinoma cells. Oncogene. 2004;23:1109-16 pubmed
immunohistochemistry - frozen section; mouse; 1:500 immunohistochemistry; mouse western blot; mouse	Nagy J, Ionescu A, Lynn B, Rash J. Coupling of astrocyte connexins Cx26, Cx30, Cx43 to oligodendrocyte Cx29, Cx32, Cx47: Implications from normal and connexin32 knockout mice. Glia. 2003;44:205-18 pubmed
immunocytochemistry; human; fig 4	Nishimura M, Saito T, Yamasaki H, Kudo R. Suppression of gap junctional intercellular communication via 5' CpG island methylation in promoter region of E-cadherin gene in endometrial cancer cells. Carcinogenesis. 2003;24:1615-23 pubmed
immunohistochemistry; rat; 10 ug/ml	Zahs K, Kofuji P, Meier C, Dermietzel R. Connexin immunoreactivity in glial cells of the rat retina. J Comp Neurol. 2003;455:531-46 pubmed
immunohistochemistry - paraffin section; human; 1:250; fig 2	Aronica E, Gorter J, Jansen G, Leenstra S, Yankaya B, Troost D. Expression of connexin 43 and connexin 32 gap-junction proteins in epilepsy-associated brain tumors and in the perilesional epileptic cortex. Acta Neuropathol. 2001;101:449-59 pubmed
western blot; human; fig 3	Ko K, Arora P, Lee W, McCulloch C. Biochemical and functional characterization of intercellular adhesion and gap junctions in fibroblasts. Am J Physiol Cell Physiol. 2000;279:C147-57 pubmed
	Sebastián Serrano A, de Diego García L, Henshall D, Engel T, Diaz Hernandez M. Haploinsufficient TNAP Mice Display Decreased Extracellular ATP Levels and Expression of Pannexin-1 Channels. Front Pharmacol. 2018;9:170 pubmed publisher
	Horowitz S, Stamper S, Simmons J. Neuronal connexin expression in the cochlear nucleus of big brown bats. Brain Res. 2008;1197:76-84 pubmed publisher
	Lahlou H, Fanjul M, Pradayrol L, Susini C, Pyronnet S. Restoration of functional gap junctions through internal ribosome entry site-dependent synthesis of endogenous connexins in density-inhibited cancer cells. Mol Cell Biol. 2005;25:4034-45 pubmed
	Solomon I, Halat T, El Maghrabi M, O Neal M. Localization of connexin26 and connexin32 in putative CO(2)-chemosensitive brainstem regions in rat. Respir Physiol. 2001;129:101-21 pubmed
	Rash J, Yasumura T, Dudek F, Nagy J. Cell-specific expression of connexins and evidence of restricted gap junctional coupling between glial cells and between neurons. J Neurosci. 2001;21:1983-2000 pubmed
	Rash J, Staines W, Yasumura T, Patel D, Furman C, Stelmack G, et al. Immunogold evidence that neuronal gap junctions in adult rat brain and spinal cord contain connexin-36 but not connexin-32 or connexin-43. Proc Natl Acad Sci U S A. 2000;97:7573-8 pubmed

product information

Product Type :

Antibody

Product Name :

Connexin 32 Monoclonal Antibody (CX-2C2)

Catalog # :

13-8200

Quantity :

100 µg

Price :

US 406.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

ELISA: Assay-dependent, Immunohistochemistry (Frozen): Assay-dependent, Western Blot: Assay-dependent

Species :

Human, Mouse, Rat

Clone :

CX-2C2

Isotype :

IgG1, kappa

Storage :

-20°C

Description :

This gene encodes a member of the gap junction protein family. The gap junction proteins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in this gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Immunogen :

Synthetic peptide corresponding to a segment of the cytoplasmic loop of rat Connexin-32.

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Immunohistochemistry (Frozen): Assay-dependent, Western Blot: Assay-dependent

Aliases :

CMTX; CMTX1; Cnx32; connexin 32; connexin-32; CX32; Cxn-32; GAP junction 28 kDa liver protein; gap junction beta-1 protein; gap junction membrane channel protein beta 1; gap junction protein beta 1; gap junction protein, beta 1; gap junction protein, beta 1, 32kDa; Gjb1

more info or order :

Invitrogen product webpage