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company name :
Invitrogen
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product type :
antibody
product name :
Connexin 26 Monoclonal Antibody (CX-12H10)
catalog :
13-8100
quantity :
100 µg
price :
US 399.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
CX-12H10
reactivity :
common marmoset, African green monkey, human, mouse, rat, dogs
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry knockout validation
more info or order :
Invitrogen product webpage
citations: 68
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry knockout validation; mouse; 1:100; fig 5
Stewart M, Bechberger J, Welch I, Naus C, Laird D. Cx26 knockout predisposes the mammary gland to primary mammary tumors in a DMBA-induced mouse model of breast cancer. Oncotarget. 2015;6:37185-99 pubmed publisher
  • immunohistochemistry; mouse; 1:500; loading ...; fig 5c
Ohgaki R, Ohmori T, Hara S, Nakagomi S, Kanai Azuma M, Kaneda Nakashima K, et al. Essential Roles of L-Type Amino Acid Transporter 1 in Syncytiotrophoblast Development by Presenting Fusogenic 4F2hc. Mol Cell Biol. 2017;37: pubmed publisher
  • immunohistochemistry; human; 1:100; loading ...; fig 1a
  • western blot; human; 1:500; loading ...; fig 6a
Coyle D, Doyle B, Murphy J, O Donnell A, Gillick J, Puri P. Expression of connexin 26 and connexin 43 is reduced in Hirschsprung's disease. J Surg Res. 2016;206:242-251 pubmed publisher
  • immunocytochemistry; human; 1:500; loading ...; fig 1
de Wolf E, van de Wiel J, Cook J, Dale N. Altered CO2 sensitivity of connexin26 mutant hemichannels in vitro. Physiol Rep. 2016;4: pubmed
  • immunohistochemistry; mouse; loading ...; fig 6d
Forsberg D, Horn Z, Tserga E, Smedler E, Silberberg G, Shvarev Y, et al. CO2-evoked release of PGE2 modulates sighs and inspiration as demonstrated in brainstem organotypic culture. elife. 2016;5: pubmed publisher
  • immunohistochemistry; African green monkey; 1:300; loading ...; fig 3a
Suzuki N, Hosoya M, Oishi N, Okano H, Fujioka M, Ogawa K. Expression pattern of wolframin, the WFS1 (Wolfram syndrome-1 gene) product, in common marmoset (Callithrix jacchus) cochlea. Neuroreport. 2016;27:833-6 pubmed publisher
  • immunohistochemistry - frozen section; common marmoset; 1:300; fig 4
Hosoya M, Fujioka M, Kobayashi R, Okano H, Ogawa K. Overlapping expression of anion exchangers in the cochlea of a non-human primate suggests functional compensation. Neurosci Res. 2016;110:1-10 pubmed publisher
  • immunohistochemistry; African green monkey; 1:300; fig 1d
Hosoya M, Fujioka M, Ogawa K, Okano H. Distinct Expression Patterns Of Causative Genes Responsible For Hereditary Progressive Hearing Loss In Non-Human Primate Cochlea. Sci Rep. 2016;6:22250 pubmed publisher
  • immunohistochemistry; mouse; 1:100; fig 3
Palombo R, Porta G, Bruno E, Provero P, Serra V, Neduri K, et al. OTX2 regulates the expression of TAp63 leading to macular and cochlear neuroepithelium development. Aging (Albany NY). 2015;7:928-36 pubmed
  • western blot; human; fig 6
Jiang H, Zhao L, Dong X, He A, Zheng C, Johansson M, et al. Tanshinone IIA enhances bystander cell killing of cancer cells expressing Drosophila melanogaster deoxyribonucleoside kinase in nuclei and mitochondria. Oncol Rep. 2015;34:1487-93 pubmed publisher
  • immunohistochemistry; rat; 1:100; fig 2
Wu X, Wang Y, Sun Y, Chen S, Zhang S, Shen L, et al. Reduced expression of Connexin26 and its DNA promoter hypermethylation in the inner ear of mimetic aging rats induced by d-galactose. Biochem Biophys Res Commun. 2014;452:340-6 pubmed publisher
  • immunohistochemistry; mouse; 1:500
  • western blot; mouse; 1:500
Bosen F, Schütz M, Beinhauer A, Strenzke N, Franz T, Willecke K. The Clouston syndrome mutation connexin30 A88V leads to hyperproliferation of sebaceous glands and hearing impairments in mice. FEBS Lett. 2014;588:1795-801 pubmed publisher
  • immunohistochemistry - frozen section; human; 1:100
Holzer L, Cor A, Holzer G. Expression of gap junction proteins connexins 26, 30, and 43 in Dupuytren's disease. Acta Orthop. 2014;85:97-101 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:250
  • western blot; human; 1:500
Yu J, Boicea A, Barrett K, James C, Bagchi I, Bagchi M, et al. Reduced connexin 43 in eutopic endometrium and cultured endometrial stromal cells from subjects with endometriosis. Mol Hum Reprod. 2014;20:260-70 pubmed publisher
  • western blot; mouse; fig 1
Xiao J, Zhang G, Qiu P, Liu X, Wu Y, Du B, et al. Tanshinone IIA increases the bystander effect of herpes simplex virus thymidine kinase/ganciclovir gene therapy via enhanced gap junctional intercellular communication. PLoS ONE. 2013;8:e67662 pubmed publisher
  • immunocytochemistry; human; fig 2
  • western blot; human; fig 2
  • immunohistochemistry - paraffin section; mouse; fig 1
Terrinoni A, Serra V, Bruno E, Strasser A, Valente E, Flores E, et al. Role of p63 and the Notch pathway in cochlea development and sensorineural deafness. Proc Natl Acad Sci U S A. 2013;110:7300-5 pubmed publisher
  • immunocytochemistry; human; fig s1
Johnson K, Mitra S, Katoch P, Kelsey L, Johnson K, Mehta P. Phosphorylation on Ser-279 and Ser-282 of connexin43 regulates endocytosis and gap junction assembly in pancreatic cancer cells. Mol Biol Cell. 2013;24:715-33 pubmed publisher
  • immunocytochemistry; mouse; 1:500; fig 3
  • western blot; mouse; 1:500
Degen J, Schütz M, Dicke N, Strenzke N, Jokwitz M, Moser T, et al. Connexin32 can restore hearing in connexin26 deficient mice. Eur J Cell Biol. 2011;90:817-24 pubmed publisher
  • immunohistochemistry; mouse; 1:500; fig 5
Schütz M, Auth T, Gehrt A, Bosen F, Körber I, Strenzke N, et al. The connexin26 S17F mouse mutant represents a model for the human hereditary keratitis-ichthyosis-deafness syndrome. Hum Mol Genet. 2011;20:28-39 pubmed publisher
  • western blot; mouse; 1:500; fig 4
Schütz M, Scimemi P, Majumder P, De Siati R, Crispino G, Rodriguez L, et al. The human deafness-associated connexin 30 T5M mutation causes mild hearing loss and reduces biochemical coupling among cochlear non-sensory cells in knock-in mice. Hum Mol Genet. 2010;19:4759-73 pubmed publisher
  • western blot; human; fig 3
Ambrosi C, Gassmann O, Pranskevich J, Boassa D, Smock A, Wang J, et al. Pannexin1 and Pannexin2 channels show quaternary similarities to connexons and different oligomerization numbers from each other. J Biol Chem. 2010;285:24420-31 pubmed publisher
  • immunohistochemistry - paraffin section; human; fig 1
  • western blot; human; 1:500; fig 4
Liu X, Furuya T, Li D, Xu J, Cao X, Li Q, et al. Connexin 26 expression correlates with less aggressive phenotype of intestinal type-gastric carcinomas. Int J Mol Med. 2010;25:709-16 pubmed
  • immunocytochemistry; human; fig s1
del Castillo F, Cohen Salmon M, Charollais A, Caille D, Lampe P, Chavrier P, et al. Consortin, a trans-Golgi network cargo receptor for the plasma membrane targeting and recycling of connexins. Hum Mol Genet. 2010;19:262-75 pubmed publisher
  • western blot; human; 1:500
Serre Beinier V, Bosco D, Zulianello L, Charollais A, Caille D, Charpantier E, et al. Cx36 makes channels coupling human pancreatic beta-cells, and correlates with insulin expression. Hum Mol Genet. 2009;18:428-39 pubmed publisher
  • western blot; human; fig 7
Schalper K, Palacios Prado N, Retamal M, Shoji K, Martinez A, Saez J. Connexin hemichannel composition determines the FGF-1-induced membrane permeability and free [Ca2+]i responses. Mol Biol Cell. 2008;19:3501-13 pubmed publisher
  • immunocytochemistry; human; fig 4
  • western blot; human; fig 3a
Leinonen P, Aaltonen V, Koskela S, Lehenkari P, Korkiamäki T, Peltonen J. Impaired gap junction formation and intercellular calcium signaling in urinary bladder cancer cells can be improved by Gö6976. Cell Commun Adhes. 2007;14:125-36 pubmed
  • immunocytochemistry; dogs
Larre I, Ponce A, Fiorentino R, Shoshani L, Contreras R, Cereijido M. Contacts and cooperation between cells depend on the hormone ouabain. Proc Natl Acad Sci U S A. 2006;103:10911-6 pubmed
  • immunocytochemistry; human; 1:250
  • western blot; human; 1:500
Gemel J, Lin X, Veenstra R, Beyer E. N-terminal residues in Cx43 and Cx40 determine physiological properties of gap junction channels, but do not influence heteromeric assembly with each other or with Cx26. J Cell Sci. 2006;119:2258-68 pubmed
  • immunocytochemistry; rat; 1:100
Gorbe A, Becker D, Dux L, Krenacs L, Krenacs T. In differentiating prefusion myoblasts connexin43 gap junction coupling is upregulated before myoblast alignment then reduced in post-mitotic cells. Histochem Cell Biol. 2006;125:705-16 pubmed
  • immunohistochemistry - paraffin section; human
Haass N, Wladykowski E, Kief S, Moll I, Brandner J. Differential induction of connexins 26 and 30 in skin tumors and their adjacent epidermis. J Histochem Cytochem. 2006;54:171-82 pubmed
  • immunocytochemistry; human; fig 6
Wang M, Martinez A, Berthoud V, Seul K, Gemel J, Valiunas V, et al. Connexin43 with a cytoplasmic loop deletion inhibits the function of several connexins. Biochem Biophys Res Commun. 2005;333:1185-93 pubmed
  • immunohistochemistry - frozen section; rat; 1:100
Gorbe A, Becker D, Dux L, Stelkovics E, Krenacs L, Bagdi E, et al. Transient upregulation of connexin43 gap junctions and synchronized cell cycle control precede myoblast fusion in regenerating skeletal muscle in vivo. Histochem Cell Biol. 2005;123:573-83 pubmed
  • immunocytochemistry; human; 1:500; fig 4
  • western blot; human; 1:500; fig 5
Saito T, Tanaka R, Wataba K, Kudo R, Yamasaki H. Overexpression of estrogen receptor-alpha gene suppresses gap junctional intercellular communication in endometrial carcinoma cells. Oncogene. 2004;23:1109-16 pubmed
  • immunocytochemistry; human; fig 3
Aasen T, Hodgins M, Edward M, Graham S. The relationship between connexins, gap junctions, tissue architecture and tumour invasion, as studied in a novel in vitro model of HPV-16-associated cervical cancer progression. Oncogene. 2003;22:7969-80 pubmed
  • immunohistochemistry - frozen section; rat
Nakashima Y, Kohno H, El Assal O, Dhar D, Ono T, Yamanoi A, et al. Sequential changes of connexin32 and connexin26 in ischemia-reperfusion of the liver in rats. Hepatol Res. 2003;27:67-75 pubmed
  • immunocytochemistry; human; fig 4
Nishimura M, Saito T, Yamasaki H, Kudo R. Suppression of gap junctional intercellular communication via 5' CpG island methylation in promoter region of E-cadherin gene in endometrial cancer cells. Carcinogenesis. 2003;24:1615-23 pubmed
  • immunohistochemistry; rat; 10 ug/ml
Zahs K, Kofuji P, Meier C, Dermietzel R. Connexin immunoreactivity in glial cells of the rat retina. J Comp Neurol. 2003;455:531-46 pubmed
  • immunohistochemistry; human
Rouan F, White T, Brown N, Taylor A, Lucke T, Paul D, et al. trans-dominant inhibition of connexin-43 by mutant connexin-26: implications for dominant connexin disorders affecting epidermal differentiation. J Cell Sci. 2001;114:2105-13 pubmed
  • western blot; human; fig 3
Ko K, Arora P, Lee W, McCulloch C. Biochemical and functional characterization of intercellular adhesion and gap junctions in fibroblasts. Am J Physiol Cell Physiol. 2000;279:C147-57 pubmed
  • immunohistochemistry - paraffin section; human; fig 5
  • immunohistochemistry - paraffin section; mouse
  • western blot; mouse; 1:300; fig 1
Ito A, Katoh F, Kataoka T, Okada M, Tsubota N, Asada H, et al. A role for heterologous gap junctions between melanoma and endothelial cells in metastasis. J Clin Invest. 2000;105:1189-97 pubmed
  • immunohistochemistry - frozen section; rat
Madon J, Hagenbuch B, Landmann L, Meier P, Stieger B. Transport function and hepatocellular localization of mrp6 in rat liver. Mol Pharmacol. 2000;57:634-41 pubmed
Hill E, Dale N, Wall M. Detecting CO2-Sensitive Hemichannels in Neurons in Acute Brain Slices. STAR Protoc. 2020;1:100139 pubmed publisher
Albuloushi A, Lovgren M, Steel A, Yeoh Y, Waters A, Zamiri M, et al. A heterozygous mutation in GJB2 (Cx26F142L) associated with deafness and recurrent skin rashes results in connexin assembly deficiencies. Exp Dermatol. 2020;29:970-979 pubmed publisher
Beach R, Abitbol J, Allman B, Esseltine J, Shao Q, Laird D. GJB2 Mutations Linked to Hearing Loss Exhibit Differential Trafficking and Functional Defects as Revealed in Cochlear-Relevant Cells. Front Cell Dev Biol. 2020;8:215 pubmed publisher
Lee M, Wang H, White T, Brooks T, Pittman A, Halai H, et al. Allele-Specific Small Interfering RNA Corrects Aberrant Cellular Phenotype in Keratitis-Ichthyosis-Deafness Syndrome Keratinocytes. J Invest Dermatol. 2020;140:1035-1044.e7 pubmed publisher
Patodia S, Paradiso B, Ellis M, Somani A, Sisodiya S, Devinsky O, et al. Characterisation of medullary astrocytic populations in respiratory nuclei and alterations in sudden unexpected death in epilepsy. Epilepsy Res. 2019;157:106213 pubmed publisher
Huebner A, Maier H, Maul A, Nietzsche S, Herrmann T, Praetorius J, et al. Early Hearing Loss upon Disruption of Slc4a10 in C57BL/6 Mice. J Assoc Res Otolaryngol. 2019;20:233-245 pubmed publisher
Bazzoun D, Adissu H, Wang L, Urazaev A, Tenvooren I, Fostok S, et al. Connexin 43 maintains tissue polarity and regulates mitotic spindle orientation in the breast epithelium. J Cell Sci. 2019;132: pubmed publisher
Kim E, Bae Y, Choi M, Hong S. Connexin 43 plays an important role in the transformation of cholangiocytes with Clonochis sinensis excretory-secretory protein and N-nitrosodimethylamine. PLoS Negl Trop Dis. 2019;13:e0006843 pubmed publisher
Iikawa N, Yamamoto Y, Kawasaki Y, Nishijima Matsunobu A, Suzuki M, Yamada T, et al. Intrinsic Oncogenic Function of Intracellular Connexin26 Protein in Head and Neck Squamous Cell Carcinoma Cells. Int J Mol Sci. 2018;19: pubmed publisher
Press E, Alaga K, Barr K, Shao Q, Bosen F, Willecke K, et al. Disease-linked connexin26 S17F promotes volar skin abnormalities and mild wound healing defects in mice. Cell Death Dis. 2017;8:e2845 pubmed publisher
Tsao D, Wang S, Lynn B, Nagy J. Immunofluorescence reveals unusual patterns of labelling for connexin43 localized to calbindin-D28K-positive interstitial cells in the pineal gland. Eur J Neurosci. 2017;45:1553-1569 pubmed publisher
Levi L, Lobo G, Doud M, von Lintig J, Seachrist D, Tochtrop G, et al. Genetic ablation of the fatty acid-binding protein FABP5 suppresses HER2-induced mammary tumorigenesis. Cancer Res. 2013;73:4770-80 pubmed publisher
Hostinar C, Stellern S, Schaefer C, Carlson S, Gunnar M. Associations between early life adversity and executive function in children adopted internationally from orphanages. Proc Natl Acad Sci U S A. 2012;109 Suppl 2:17208-12 pubmed publisher
Martinez A, Maripillan J, Acuña R, Minogue P, Berthoud V, Beyer E. Different domains are critical for oligomerization compatibility of different connexins. Biochem J. 2011;436:35-43 pubmed publisher
Nomura S, Maeda K, Noda E, Inoue T, Fukunaga S, Nagahara H, et al. Clinical significance of the expression of connexin26 in colorectal cancer. J Exp Clin Cancer Res. 2010;29:79 pubmed publisher
Sun Y, Tang W, Chang Q, Wang Y, Kong W, Lin X. Connexin30 null and conditional connexin26 null mice display distinct pattern and time course of cellular degeneration in the cochlea. J Comp Neurol. 2009;516:569-79 pubmed publisher
Ezumi K, Yamamoto H, Murata K, Higashiyama M, Damdinsuren B, Nakamura Y, et al. Aberrant expression of connexin 26 is associated with lung metastasis of colorectal cancer. Clin Cancer Res. 2008;14:677-84 pubmed publisher
Horowitz S, Stamper S, Simmons J. Neuronal connexin expression in the cochlear nucleus of big brown bats. Brain Res. 2008;1197:76-84 pubmed publisher
Kandyba E, Hodgins M, Martin P. A murine living skin equivalent amenable to live-cell imaging: analysis of the roles of connexins in the epidermis. J Invest Dermatol. 2008;128:1039-49 pubmed
Ikeda Y, Fry C, Hayashi F, Stolz D, Griffiths D, Kanai A. Role of gap junctions in spontaneous activity of the rat bladder. Am J Physiol Renal Physiol. 2007;293:F1018-25 pubmed
Lahlou H, Fanjul M, Pradayrol L, Susini C, Pyronnet S. Restoration of functional gap junctions through internal ribosome entry site-dependent synthesis of endogenous connexins in density-inhibited cancer cells. Mol Cell Biol. 2005;25:4034-45 pubmed
Gemel J, Valiunas V, Brink P, Beyer E. Connexin43 and connexin26 form gap junctions, but not heteromeric channels in co-expressing cells. J Cell Sci. 2004;117:2469-80 pubmed
Thomas T, Telford D, Laird D. Functional domain mapping and selective trans-dominant effects exhibited by Cx26 disease-causing mutations. J Biol Chem. 2004;279:19157-68 pubmed
Walcott B, Moore L, Birzgalis A, Claros N, Valiunas V, Ott T, et al. Role of gap junctions in fluid secretion of lacrimal glands. Am J Physiol Cell Physiol. 2002;282:C501-7 pubmed
Solomon I, Halat T, El Maghrabi M, O Neal M. Localization of connexin26 and connexin32 in putative CO(2)-chemosensitive brainstem regions in rat. Respir Physiol. 2001;129:101-21 pubmed
Rash J, Yasumura T, Dudek F, Nagy J. Cell-specific expression of connexins and evidence of restricted gap junctional coupling between glial cells and between neurons. J Neurosci. 2001;21:1983-2000 pubmed
Bruzzone S, Guida L, Zocchi E, Franco L, De Flora A -. Connexin 43 hemi channels mediate Ca2+-regulated transmembrane NAD+ fluxes in intact cells. FASEB J. 2001;15:10-12 pubmed
product information
Product Type :
Antibody
Product Name :
Connexin 26 Monoclonal Antibody (CX-12H10)
Catalog # :
13-8100
Quantity :
100 µg
Price :
US 399.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Human, Mouse, Rat
Applications :
ELISA: 0.1-1 µg/mL, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry: 1-2 µg/mL, Western Blot: 1-2 µg/mL
Species :
Human, Mouse, Rat
Clone :
CX-12H10
Isotype :
IgG1, kappa
Storage :
-20°C
Description :
Gap junctions are conduits that allow the direct cell-to-cell passage of small cytoplasmic molecules, including ions, metabolic intermediates, and second messengers, and thereby mediate intercellular metabolic and electrical communication. Gap junction channels consist of connexin protein subunits, which are encoded by a multigene family. GJBs (gap-junction proteins or connexins) play crucial functional roles associated with these channels. Defects in GJB3 have been linked to erythrokeratodermia variables (EKV) is an autosomal dominant genodermatosis characterized by transient figurate red patches or hyperkeratosis. Mutations in GJB2 have also been associated with genetically derived hearing impairments, including autosomal recessive nonsyndromic deafness.
Immunogen :
Synthetic peptide corresponding to a segment of the cytoplasmic loop of rat Connexin-26.
Format :
Liquid
Applications w/Dilutions :
ELISA: 0.1-1 µg/mL, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry: 1-2 µg/mL, Western Blot: 1-2 µg/mL
Aliases :
AI325222; Cnx26; connexin 26; connexin-26; CX26; CXN-26; DFNA3; DFNA3A; DFNB1; DFNB1A; Gap junction beta-2 protein; gap junction channel protein connexin 26; gap junction membrane channel protein beta 2; gap junction protein beta 2; gap junction protein, beta 2; gap junction protein, beta 2, 26kDa; GJB2; Gjb-2; HID; KID; mutant gap junction protein beta 2; NSRD1; PPK
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA