IL-10 Monoclonal Antibody (JES3-12G8), Biotin, eBioscience | Invitrogen 13-7109-85 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

IL-10 Monoclonal Antibody (JES3-12G8), Biotin, eBioscience

catalog :

13-7109-85

quantity :

500 µg

price :

US 368

clonality :

monoclonal

host :

rat

conjugate :

biotin

clone name :

JES3-12G8

reactivity :

human

application :

ELISA

more info or order :

Invitrogen product webpage

citations: 14

Reference
Carestia A, Mena H, Olexen C, Ortiz Wilczyñski J, Negrotto S, Errasti A, et al. Platelets Promote Macrophage Polarization toward Pro-inflammatory Phenotype and Increase Survival of Septic Mice. Cell Rep. 2019;28:896-908.e5 pubmed publisher
Kim J, Bae S, Song S, Chung K, Kwon S. Fiber composite slices for multiplexed immunoassays. Biomicrofluidics. 2015;9:044109 pubmed publisher
Lavoy E, Bosch J, Lowder T, Simpson R. Acute aerobic exercise in humans increases cytokine expression in CD27(-) but not CD27(+) CD8(+) T-cells. Brain Behav Immun. 2013;27:54-62 pubmed publisher
Gellatly S, Needham B, Madera L, Trent M, Hancock R. The Pseudomonas aeruginosa PhoP-PhoQ two-component regulatory system is induced upon interaction with epithelial cells and controls cytotoxicity and inflammation. Infect Immun. 2012;80:3122-31 pubmed publisher
Vohra P, Poxton I. Induction of cytokines in a macrophage cell line by proteins of Clostridium difficile. FEMS Immunol Med Microbiol. 2012;65:96-104 pubmed publisher
Sauer U, Domnanich P, Preininger C. Protein chip for the parallel quantification of high and low abundant biomarkers for sepsis. Anal Biochem. 2011;419:46-52 pubmed publisher
James E, Van Haren S, Ettinger R, Fijnvandraat K, Liberman J, Kwok W, et al. T-cell responses in two unrelated hemophilia A inhibitor subjects include an epitope at the factor VIII R593C missense site. J Thromb Haemost. 2011;9:689-99 pubmed publisher
Domnanich P, Peña D, Preininger C. Xanthan/chitosan gold chip for metal enhanced protein biomarker detection. Biosens Bioelectron. 2011;26:2559-65 pubmed publisher
Yadav M, Liebau E, Haldar C, Rathaur S. Identification of major antigenic peptide of filarial glutathione-S-transferase. Vaccine. 2011;29:1297-303 pubmed publisher
Shi Z, Okuno Y, Rifa i M, Endharti A, Akane K, Isobe K, et al. Human CD8+CXCR3+ T cells have the same function as murine CD8+CD122+ Treg. Eur J Immunol. 2009;39:2106-19 pubmed publisher
Reske A, Pollara G, Krummenacher C, Katz D, Chain B. Glycoprotein-dependent and TLR2-independent innate immune recognition of herpes simplex virus-1 by dendritic cells. J Immunol. 2008;180:7525-36 pubmed
Nowacki T, Kuerten S, Zhang W, Shive C, Kreher C, Boehm B, et al. Granzyme B production distinguishes recently activated CD8(+) memory cells from resting memory cells. Cell Immunol. 2007;247:36-48 pubmed
Quast S, Zhang W, Shive C, Kovalovski D, Ott P, Herzog B, et al. IL-2 absorption affects IFN-gamma and IL-5, but not IL-4 producing memory T cells in double color cytokine ELISPOT assays. Cell Immunol. 2005;237:28-36 pubmed
Abrams J, Roncarolo M, Yssel H, Andersson U, Gleich G, Silver J. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992;127:5-24 pubmed

product information

Product Type :

Antibody

Product Name :

IL-10 Monoclonal Antibody (JES3-12G8), Biotin, eBioscience

Catalog # :

13-7109-85

Quantity :

500 µg

Price :

US 368

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Rat

Reactivity :

Human

Applications :

ELISA: 0.5-2 µg/mL

Species :

Human

Clone :

JES3-12G8

Isotype :

IgG2a, kappa

Storage :

4° C, store in dark, DO NOT FREEZE!

Description :

Interleukin 10 (IL-10, CSIF) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 is an 18.6 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein (BCRFI). The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells, enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells. Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). IL-10 inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Knockout studies in mice suggest the function of this cytokine as an essential immunoregulator in the intestinal tract.

Format :

Liquid

Applications w/Dilutions :

ELISA: 0.5-2 µg/mL

Aliases :

CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF

more info or order :

Invitrogen product webpage