STAT3 Monoclonal Antibody (ST3-5G7) | Invitrogen 13-7000 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

STAT3 Monoclonal Antibody (ST3-5G7)

catalog :

13-7000

quantity :

100µg

price :

US 331

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

ST3-5G7

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section, ChIP-Seq, western blot knockout validation

citations: 19

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; fig S1	Schlessinger K, Levy D. Malignant transformation but not normal cell growth depends on signal transducer and activator of transcription 3. Cancer Res. 2005;65:5828-34 pubmed
ChIP-Seq; mouse; loading ...; fig 1c immunoprecipitation; mouse; loading ...; fig 2f	Cheung K, Zhang F, Jaganathan A, Sharma R, Zhang Q, Konuma T, et al. Distinct Roles of Brd2 and Brd4 in Potentiating the Transcriptional Program for Th17 Cell Differentiation. Mol Cell. 2017;65:1068-1080.e5 pubmed publisher
western blot; human; loading ...; fig 3c	Govender U, Corre B, Bourdache Y, Pellegrini S, Michel F. Type I interferon-enhanced IL-10 expression in human CD4 T cells is regulated by STAT3, STAT2, and BATF transcription factors. J Leukoc Biol. 2017;101:1181-1190 pubmed publisher
ChIP-Seq; mouse; fig 3	Wan C, Li P, Spolski R, Oh J, Andraski A, Du N, et al. IL-21-mediated non-canonical pathway for IL-1β production in conventional dendritic cells. Nat Commun. 2015;6:7988 pubmed publisher
EMSA; human; 1 ug	Chan S, Rickert C, Vermi W, Sheehan K, Arthur C, Allen J, et al. Dysregulated STAT1-SOCS1 control of JAK2 promotes mammary luminal progenitor cell survival and drives ER?(+) tumorigenesis. Cell Death Differ. 2014;21:234-46 pubmed publisher
western blot; human	Barnes N, Stephenson S, Cocco M, Tooze R, Doody G. BLIMP-1 and STAT3 counterregulate microRNA-21 during plasma cell differentiation. J Immunol. 2012;189:253-60 pubmed publisher
EMSA; mouse	Mermel C, McLemore M, Liu F, Pereira S, Woloszynek J, Lowell C, et al. Src family kinases are important negative regulators of G-CSF-dependent granulopoiesis. Blood. 2006;108:2562-8 pubmed
western blot; human	Heinrich M, McArthur G, Demetri G, Joensuu H, Bono P, Herrmann R, et al. Clinical and molecular studies of the effect of imatinib on advanced aggressive fibromatosis (desmoid tumor). J Clin Oncol. 2006;24:1195-203 pubmed
western blot; human	Piva R, Chiarle R, Manazza A, Taulli R, Simmons W, Ambrogio C, et al. Ablation of oncogenic ALK is a viable therapeutic approach for anaplastic large-cell lymphomas. Blood. 2006;107:689-97 pubmed
chromatin immunoprecipitation; human; fig 4 western blot; human; fig 4	Ghosh M, Sharma P, Harbor P, Rahaman S, Haque S. PI3K-AKT pathway negatively controls EGFR-dependent DNA-binding activity of Stat3 in glioblastoma multiforme cells. Oncogene. 2005;24:7290-300 pubmed
immunoprecipitation; mouse immunocytochemistry; mouse; 1:200; fig 3 western blot; mouse; 1:1000 immunohistochemistry - paraffin section; human; 1:200; fig 1 immunocytochemistry; human; 1:200 western blot; human; 1:1000	Silver D, Naora H, Liu J, Cheng W, Montell D. Activated signal transducer and activator of transcription (STAT) 3: localization in focal adhesions and function in ovarian cancer cell motility. Cancer Res. 2004;64:3550-8 pubmed
western blot; human; fig 2	Duensing A, Medeiros F, McConarty B, Joseph N, Panigrahy D, Singer S, et al. Mechanisms of oncogenic KIT signal transduction in primary gastrointestinal stromal tumors (GISTs). Oncogene. 2004;23:3999-4006 pubmed
immunoprecipitation; human; fig 2	Nepomuceno R, Snow A, Robert Beatty P, Krams S, Martinez O. Constitutive activation of Jak/STAT proteins in Epstein-Barr virus-infected B-cell lines from patients with posttransplant lymphoproliferative disorder. Transplantation. 2002;74:396-402 pubmed
EMSA; human	Dumoutier L, Van Roost E, Colau D, Renauld J. Human interleukin-10-related T cell-derived inducible factor: molecular cloning and functional characterization as an hepatocyte-stimulating factor. Proc Natl Acad Sci U S A. 2000;97:10144-9 pubmed
EMSA; mouse; fig 8, 9	Dumoutier L, Louahed J, Renauld J. Cloning and characterization of IL-10-related T cell-derived inducible factor (IL-TIF), a novel cytokine structurally related to IL-10 and inducible by IL-9. J Immunol. 2000;164:1814-9 pubmed
western blot; human; fig 1	Fernandes A, Hamburger A, Gerwin B. ErbB-2 kinase is required for constitutive stat 3 activation in malignant human lung epithelial cells. Int J Cancer. 1999;83:564-70 pubmed
EMSA; mouse; fig 2	Demoulin J, Van Roost E, Stevens M, Groner B, Renauld J. Distinct roles for STAT1, STAT3, and STAT5 in differentiation gene induction and apoptosis inhibition by interleukin-9. J Biol Chem. 1999;274:25855-61 pubmed
EMSA; mouse; fig 5a immunoprecipitation; mouse; fig 5b western blot; human; fig 5b	Raz R, Lee C, Cannizzaro L, D EUSTACHIO P, Levy D. Essential role of STAT3 for embryonic stem cell pluripotency. Proc Natl Acad Sci U S A. 1999;96:2846-51 pubmed
	Wan C, Andraski A, Spolski R, Li P, Kazemian M, Oh J, et al. Opposing roles of STAT1 and STAT3 in IL-21 function in CD4+ T cells. Proc Natl Acad Sci U S A. 2015;112:9394-9 pubmed publisher

product information

Product Type :

Antibody

Product Name :

STAT3 Monoclonal Antibody (ST3-5G7)

Catalog # :

13-7000

Quantity :

100µg

Price :

US 331

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

ChIP assay: Assay Dependent, ELISA: Assay Dependent, Gel Shift: Assay Dependent, Immunocytochemistry: 1:250, Immunofluorescence: 1:250, Immunohistochemistry (Paraffin): 1:20-1:500, Immunoprecipitation: Assay Dependent, Western Blot: 2 µg/mL

Species :

Human, Mouse, Rat

Clone :

ST3-5G7

Isotype :

IgG1

Storage :

-20°C

Description :

STAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the nucleus, and act as transcription factors in response to cytokines and growth factors. Coactivators such as CREB-binding protein are required for the transcriptional activation by STAT3. STAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6. Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity. In humans, the STAT3 gene is located on the q arm of chromosome 17. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3 while the PIAS3 protein is a specific inhibitor of STAT3. Three alternatively spliced transcript variants encoding distinct isoforms of STAT3 have been described. Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.

Immunogen :

A GST-fusion protein containing the unique carboxy terminal domain of the murine STAT3 protein.

Format :

Liquid

Applications w/Dilutions :

Aliases :

Signal transducer and activator of transcription 3, Acute-phase response factor, STAT3, APRF