This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
GFP Monoclonal Antibody (5F12.4), Biotin, eBioscience
catalog :
13-6498-82
quantity :
100 ug
price :
US 395.00
clonality :
monoclonal
host :
rat
conjugate :
biotin
clone name :
5F12.4
application :
flow cytometry
citations: 4
Reference
Graham M, Abram C, Hu Y, Lowell C. Expression of the TEL-Syk fusion protein in hematopoietic stem cells leads to rapidly fatal myelofibrosis in mice. PLoS ONE. 2013;8:e77542 pubmed publisher
Ebe T. [Green fluorescent protein as a marker gene expression]. Tanpakushitsu Kakusan Koso. 2007;52:1766-7 pubmed
Cormack B, Valdivia R, Falkow S. FACS-optimized mutants of the green fluorescent protein (GFP). Gene. 1996;173:33-8 pubmed
Rizzuto R, Brini M, De Giorgi F, Rossi R, Heim R, Tsien R, et al. Double labelling of subcellular structures with organelle-targeted GFP mutants in vivo. Curr Biol. 1996;6:183-8 pubmed
product information
Product Type :
Antibody
Product Name :
GFP Monoclonal Antibody (5F12.4), Biotin, eBioscience
Catalog # :
13-6498-82
Quantity :
100 ug
Price :
US 395.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Tag
Applications :
Flow Cytometry: 0.5 ug/test
Species :
Tag
Clone :
5F12.4
Isotype :
IgG2a, kappa
Storage :
4 C, store in dark, DO NOT FREEZE!
Description :
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.5 ug/test
Aliases :
GFP; GFP tag; GFP2; green fluorescence; green fluorescent; Turbo eGFP
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA